Effects of Iron on Efficacy of Photodynamic Therapy Using Photolon in a Mouse Model of CT26 Colon Cancer
Background: Photodynamic therapy (PDT) -a minimally invasive anti-cancer therapy-is undergoing experimental studies to increase its anti-cancer effects. This study investigated the influence of iron on the anti-cancer effects of PDT. Methods: PDT was performed in a cancer-bearing mouse model, which...
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| Veröffentlicht in: | Journal of Nippon Medical School 2023/02/25, Vol.90(1), pp.41-49 |
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| creator | Lee, Jung In Ahn, Tae-Gyu Choi, Ji Hyun |
| description | Background: Photodynamic therapy (PDT) -a minimally invasive anti-cancer therapy-is undergoing experimental studies to increase its anti-cancer effects. This study investigated the influence of iron on the anti-cancer effects of PDT. Methods: PDT was performed in a cancer-bearing mouse model, which was created by using a murine colon carcinoma (CT26) cell line after administration of Photolon and iron. Tumor volume and the results of TdT-mediated dUTP-biotin nick end labeling (TUNEL), 8-OHdG, and TBARS assays were used to measure anti-cancer effect. Results: On day 14, tumor volume had increased by 49% in the PDT group and decreased by 72% in the iron+PDT group. The percentage of TUNEL-positive cells in tumor tissues was 45% in the PDT group and 69% in the iron+PDT group, suggesting that the proportion of TUNEL-positive cells had increased in the iron+PDT group. The 8-OHdG content in tumor tissues was 33% higher in the iron+PDT group than in the PDT group. The TBARS content in tumor tissues was 46% higher in the iron+PDT group than in the PDT group. Conclusions: Iron enhances the anti-cancer effect of PDT using Photolon, most likely by increasing oxidative damage. |
| doi_str_mv | 10.1272/jnms.JNMS.2023_90-108 |
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This study investigated the influence of iron on the anti-cancer effects of PDT. Methods: PDT was performed in a cancer-bearing mouse model, which was created by using a murine colon carcinoma (CT26) cell line after administration of Photolon and iron. Tumor volume and the results of TdT-mediated dUTP-biotin nick end labeling (TUNEL), 8-OHdG, and TBARS assays were used to measure anti-cancer effect. Results: On day 14, tumor volume had increased by 49% in the PDT group and decreased by 72% in the iron+PDT group. The percentage of TUNEL-positive cells in tumor tissues was 45% in the PDT group and 69% in the iron+PDT group, suggesting that the proportion of TUNEL-positive cells had increased in the iron+PDT group. The 8-OHdG content in tumor tissues was 33% higher in the iron+PDT group than in the PDT group. The TBARS content in tumor tissues was 46% higher in the iron+PDT group than in the PDT group. Conclusions: Iron enhances the anti-cancer effect of PDT using Photolon, most likely by increasing oxidative damage.</description><identifier>ISSN: 1345-4676</identifier><identifier>EISSN: 1347-3409</identifier><identifier>DOI: 10.1272/jnms.JNMS.2023_90-108</identifier><identifier>PMID: 36908127</identifier><language>eng</language><publisher>Japan: The Medical Association of Nippon Medical School</publisher><subject>Animals ; anti-cancer ; Apoptosis ; Cell Line, Tumor ; Colonic Neoplasms ; Disease Models, Animal ; Iron ; Mice ; Photochemotherapy ; photodynamic therapy ; Photosensitizing Agents - pharmacology ; Photosensitizing Agents - therapeutic use ; Porphyrins - pharmacology ; Thiobarbituric Acid Reactive Substances - pharmacology</subject><ispartof>Journal of Nippon Medical School, 2023/02/25, Vol.90(1), pp.41-49</ispartof><rights>2023 by the Medical Association of Nippon Medical School</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c635t-5b96db582b9f888a6ae3974ac141d085d06e8fd311e921f9f1a36e3f2a2eda6e3</citedby><cites>FETCH-LOGICAL-c635t-5b96db582b9f888a6ae3974ac141d085d06e8fd311e921f9f1a36e3f2a2eda6e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36908127$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Jung In</creatorcontrib><creatorcontrib>Ahn, Tae-Gyu</creatorcontrib><creatorcontrib>Choi, Ji Hyun</creatorcontrib><creatorcontrib>Department of Obstetrics and Gynecology</creatorcontrib><creatorcontrib>Chosun University College of Medicine</creatorcontrib><creatorcontrib>Department of Internal Medicine</creatorcontrib><title>Effects of Iron on Efficacy of Photodynamic Therapy Using Photolon in a Mouse Model of CT26 Colon Cancer</title><title>Journal of Nippon Medical School</title><addtitle>J Nippon Med Sch</addtitle><description>Background: Photodynamic therapy (PDT) -a minimally invasive anti-cancer therapy-is undergoing experimental studies to increase its anti-cancer effects. This study investigated the influence of iron on the anti-cancer effects of PDT. Methods: PDT was performed in a cancer-bearing mouse model, which was created by using a murine colon carcinoma (CT26) cell line after administration of Photolon and iron. Tumor volume and the results of TdT-mediated dUTP-biotin nick end labeling (TUNEL), 8-OHdG, and TBARS assays were used to measure anti-cancer effect. Results: On day 14, tumor volume had increased by 49% in the PDT group and decreased by 72% in the iron+PDT group. The percentage of TUNEL-positive cells in tumor tissues was 45% in the PDT group and 69% in the iron+PDT group, suggesting that the proportion of TUNEL-positive cells had increased in the iron+PDT group. The 8-OHdG content in tumor tissues was 33% higher in the iron+PDT group than in the PDT group. The TBARS content in tumor tissues was 46% higher in the iron+PDT group than in the PDT group. Conclusions: Iron enhances the anti-cancer effect of PDT using Photolon, most likely by increasing oxidative damage.</description><subject>Animals</subject><subject>anti-cancer</subject><subject>Apoptosis</subject><subject>Cell Line, Tumor</subject><subject>Colonic Neoplasms</subject><subject>Disease Models, Animal</subject><subject>Iron</subject><subject>Mice</subject><subject>Photochemotherapy</subject><subject>photodynamic therapy</subject><subject>Photosensitizing Agents - pharmacology</subject><subject>Photosensitizing Agents - therapeutic use</subject><subject>Porphyrins - pharmacology</subject><subject>Thiobarbituric Acid Reactive Substances - pharmacology</subject><issn>1345-4676</issn><issn>1347-3409</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdUE2P0zAQtRCIXRZ-AihHLin-iB37iKLdZb8Aie7Zcp3xNlHiFDsV6r_HbkoPSLZnNPPmzfND6CPBK0Jr-qX3Y1zdf3_6taKYMq1wSbB8hS4Jq-qSVVi9Pua8rEQtLtC7GHuMGeNcvEUXTCgsE80l2l47B3aOxeSKuzD5Ip1U6qyxh1z7uZ3mqT14M3a2WG8hmN2heI6df1laQ8J3vjDF07SPkN4WhjzXrKkommO7Md5CeI_eODNE-HCKV-j55nrdfCsff9zeNV8fSysYn0u-UaLdcEk3ykkpjTDAVF0ZSyrSYslbLEC6lhECihKnHDFMAHPUUGhNyq7Q54V3F6bfe4izHrtoYRiMhyRR01oKTghWJEH5ArVhijGA07vQjSYcNME6m6yzyTqbrE8mp45Mc59OK_abEdrz1D9XE-B2AaRucjKZMHQedD_tg09_1_YP6adMfWTFWGFMUhDpVjmpVEW4kJVKTA8LUx9n8wLnVSbMnR1gEZhl5ed_oWeU3ZqgwbO_kMeqGA</recordid><startdate>20230225</startdate><enddate>20230225</enddate><creator>Lee, Jung In</creator><creator>Ahn, Tae-Gyu</creator><creator>Choi, Ji Hyun</creator><general>The Medical Association of Nippon Medical School</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20230225</creationdate><title>Effects of Iron on Efficacy of Photodynamic Therapy Using Photolon in a Mouse Model of CT26 Colon Cancer</title><author>Lee, Jung In ; Ahn, Tae-Gyu ; Choi, Ji Hyun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c635t-5b96db582b9f888a6ae3974ac141d085d06e8fd311e921f9f1a36e3f2a2eda6e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>anti-cancer</topic><topic>Apoptosis</topic><topic>Cell Line, Tumor</topic><topic>Colonic Neoplasms</topic><topic>Disease Models, Animal</topic><topic>Iron</topic><topic>Mice</topic><topic>Photochemotherapy</topic><topic>photodynamic therapy</topic><topic>Photosensitizing Agents - pharmacology</topic><topic>Photosensitizing Agents - therapeutic use</topic><topic>Porphyrins - pharmacology</topic><topic>Thiobarbituric Acid Reactive Substances - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Jung In</creatorcontrib><creatorcontrib>Ahn, Tae-Gyu</creatorcontrib><creatorcontrib>Choi, Ji Hyun</creatorcontrib><creatorcontrib>Department of Obstetrics and Gynecology</creatorcontrib><creatorcontrib>Chosun University College of Medicine</creatorcontrib><creatorcontrib>Department of Internal Medicine</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Nippon Medical School</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Jung In</au><au>Ahn, Tae-Gyu</au><au>Choi, Ji Hyun</au><aucorp>Department of Obstetrics and Gynecology</aucorp><aucorp>Chosun University College of Medicine</aucorp><aucorp>Department of Internal Medicine</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Iron on Efficacy of Photodynamic Therapy Using Photolon in a Mouse Model of CT26 Colon Cancer</atitle><jtitle>Journal of Nippon Medical School</jtitle><addtitle>J Nippon Med Sch</addtitle><date>2023-02-25</date><risdate>2023</risdate><volume>90</volume><issue>1</issue><spage>41</spage><epage>49</epage><pages>41-49</pages><artnum>JNMS.2023_90-108</artnum><issn>1345-4676</issn><eissn>1347-3409</eissn><abstract>Background: Photodynamic therapy (PDT) -a minimally invasive anti-cancer therapy-is undergoing experimental studies to increase its anti-cancer effects. This study investigated the influence of iron on the anti-cancer effects of PDT. Methods: PDT was performed in a cancer-bearing mouse model, which was created by using a murine colon carcinoma (CT26) cell line after administration of Photolon and iron. Tumor volume and the results of TdT-mediated dUTP-biotin nick end labeling (TUNEL), 8-OHdG, and TBARS assays were used to measure anti-cancer effect. Results: On day 14, tumor volume had increased by 49% in the PDT group and decreased by 72% in the iron+PDT group. The percentage of TUNEL-positive cells in tumor tissues was 45% in the PDT group and 69% in the iron+PDT group, suggesting that the proportion of TUNEL-positive cells had increased in the iron+PDT group. The 8-OHdG content in tumor tissues was 33% higher in the iron+PDT group than in the PDT group. The TBARS content in tumor tissues was 46% higher in the iron+PDT group than in the PDT group. Conclusions: Iron enhances the anti-cancer effect of PDT using Photolon, most likely by increasing oxidative damage.</abstract><cop>Japan</cop><pub>The Medical Association of Nippon Medical School</pub><pmid>36908127</pmid><doi>10.1272/jnms.JNMS.2023_90-108</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese |
| subjects | Animals anti-cancer Apoptosis Cell Line, Tumor Colonic Neoplasms Disease Models, Animal Iron Mice Photochemotherapy photodynamic therapy Photosensitizing Agents - pharmacology Photosensitizing Agents - therapeutic use Porphyrins - pharmacology Thiobarbituric Acid Reactive Substances - pharmacology |
| title | Effects of Iron on Efficacy of Photodynamic Therapy Using Photolon in a Mouse Model of CT26 Colon Cancer |
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