A flow cytometry-based protocol for syngenic isolation of neurovascular unit cells from mouse and human tissues

The neurovascular unit (NVU), composed of endothelial cells, pericytes, juxtaposed astrocytes and microglia together with neurons, is essential for proper central nervous system functioning. The NVU critically regulates blood–brain barrier (BBB) function, which is impaired in several neurological di...

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Veröffentlicht in:	Nature protocols 2023-05, Vol.18 (5), p.1510-1542
Hauptverfasser:	Spitzer, Daniel, Khel, Maryam I., Pütz, Tim, Zinke, Jenny, Jia, Xiaoxiong, Sommer, Kathleen, Filipski, Katharina, Thorsen, Frits, Freiman, Thomas M., Günther, Stefan, Plate, Karl H., Harter, Patrick N., Liebner, Stefan, Reiss, Yvonne, Di Tacchio, Mariangela, Guérit, Sylvaine, Devraj, Kavi
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1407/1492 631/378/1341 692/308/53/2421 692/617/375/1370/534 692/699/375/1922 Analytical Chemistry Animals Astrocytes Biological Techniques Biomedical and Life Sciences Blood-brain barrier Blood-Brain Barrier - metabolism Cell culture Central nervous system Computational Biology/Bioinformatics Endothelial cells Endothelial Cells - physiology Flow Cytometry Human tissues Humans Immune system Life Sciences Mice Microarrays Microglia Neurological diseases Neurons Organic Chemistry Pericytes Proteomics Protocol Therapeutic targets Transcriptomics
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