Agarose native gel electrophoresis analysis of thermal aggregation controlled by Hofmeister series

The effects of salting-in and salting-out salts defined by Hofmeister series on the solution state of bovine serum albumin (BSA) in 50 mM Tris-HCl buffer at pH 7.4 before and after thermal unfolding at 80 °C for 5 min were examined using agarose native gel electrophoresis and mass photometry. Gel el...

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Veröffentlicht in:	Biophysical chemistry 2023-05, Vol.296, p.106977-106977, Article 106977
Hauptverfasser:	Tomioka, Yui, Sato, Ryo, Takahashi, Ryo, Nagatoishi, Satoru, Shiba, Kohei, Tsumoto, Kouhei, Arakawa, Tsutomu, Akuta, Teruo
Format:	Artikel
Sprache:	eng
Schlagworte:	Agarose gel Electrophoresis, Agar Gel - methods Hofmeister Mass photometry Native electrophoresis Sepharose Serum Albumin, Bovine - chemistry Sodium Chloride - chemistry Thiocyanates Tromethamine
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