In vitro complete differentiation of human spermatogonial stem cells to morphologic spermatozoa using a hybrid hydrogel of agarose and laminin
Spermatogenesis refers to the differentiation of the spermatogonial stem cells (SSCs) located in the base seminiferous tubules into haploid spermatozoa. Prerequisites for in vitro spermatogenesis include an extracellular matrix (ECM), paracrine factors, and testicular somatic cells which play a supp...
Gespeichert in:
| Veröffentlicht in: | International journal of biological macromolecules 2023-04, Vol.235, p.123801-123801, Article 123801 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 123801 |
|---|---|
| container_issue | |
| container_start_page | 123801 |
| container_title | International journal of biological macromolecules |
| container_volume | 235 |
| creator | Jabari, Ayob Gholami, Keykavos Khadivi, Farnaz Koruji, Morteza Amidi, Fardin Gilani, Mohammad Ali Sadighi Mahabadi, Vahid Pirhajati Nikmahzar, Aghbibi Salem, Maryam Movassagh, Sepideh Ashouri Feizollahi, Narjes Abbasi, Mehdi |
| description | Spermatogenesis refers to the differentiation of the spermatogonial stem cells (SSCs) located in the base seminiferous tubules into haploid spermatozoa. Prerequisites for in vitro spermatogenesis include an extracellular matrix (ECM), paracrine factors, and testicular somatic cells which play a supporting role for SSCs. Thus, the present study evaluated the potential of co-culturing Sertoli cells and SSCs embedded in a hybrid hydrogel of agarose and laminin, the main components of the ECM. Following the three–week conventional culture of human testicular cells, the cells were cultured in agarose hydrogel or agarose/laminin one (hybrid) for 74 days. Then, immunocytochemistry, real-time PCR, electron microscopy, and morphological staining methods were applied to analyze the presence of SSCs, as well as the other cells of the different stages of spermatogenesis. Based on the results, the colonies with positive spermatogenesis markers were observed in both culture systems. The existence of the cells of all three phases of spermatogenesis (spermatogonia, meiosis, and spermiogenesis) was confirmed in the two groups, while morphological spermatozoa were detected only in the hybrid hydrogel group. Finally, a biologically improved 3D matrix can support all the physiological activities of SSCs such as survival, proliferation, and differentiation. |
| doi_str_mv | 10.1016/j.ijbiomac.2023.123801 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2780487190</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0141813023006955</els_id><sourcerecordid>2834212173</sourcerecordid><originalsourceid>FETCH-LOGICAL-c449t-3f03b164f3c39d16f09d93f94fd28e96c1081d7dc1b959c24e15a558d263a5fc3</originalsourceid><addsrcrecordid>eNqFkU1r3DAQhkVpabZp_0LQsRdv9WVbvrWEfgQCubRnIUsjrxZLciU5kP6I_uZ42STXnF4Ynplh5kHoipI9JbT7ctz74-hT0GbPCON7yrgk9A3aUdkPDSGEv0U7QgVtJOXkAn0o5bhVu5bK9-iCd1KwXpAd-n8T8b2vOWGTwjJDBWy9c5AhVq-rTxEnhw9r0BGXBXLQNU0pej3jUiFgA_NccE04pLwc0pwmb17Af0njtfg4YY0PD2P2dgub0wTzaaqedE4FsI4Wzzr46ONH9M7pucCnp7xEf358_339q7m9-3lz_e22MUIMteGO8JF2wnHDB0s7RwY7cDcIZ5mEoTOUSGp7a-g4tINhAmir21Za1nHdOsMv0efz3CWnvyuUqoIvp1t0hLQWxSQXjDLa89fRXhIhezqQDe3OqNnuKhmcWrIPOj8oStRJmzqqZ23qpE2dtW2NV0871jGAfWl79rQBX88AbE-595BVMR6iAeszmKps8q_teAQhn67i</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2780487190</pqid></control><display><type>article</type><title>In vitro complete differentiation of human spermatogonial stem cells to morphologic spermatozoa using a hybrid hydrogel of agarose and laminin</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Jabari, Ayob ; Gholami, Keykavos ; Khadivi, Farnaz ; Koruji, Morteza ; Amidi, Fardin ; Gilani, Mohammad Ali Sadighi ; Mahabadi, Vahid Pirhajati ; Nikmahzar, Aghbibi ; Salem, Maryam ; Movassagh, Sepideh Ashouri ; Feizollahi, Narjes ; Abbasi, Mehdi</creator><creatorcontrib>Jabari, Ayob ; Gholami, Keykavos ; Khadivi, Farnaz ; Koruji, Morteza ; Amidi, Fardin ; Gilani, Mohammad Ali Sadighi ; Mahabadi, Vahid Pirhajati ; Nikmahzar, Aghbibi ; Salem, Maryam ; Movassagh, Sepideh Ashouri ; Feizollahi, Narjes ; Abbasi, Mehdi</creatorcontrib><description>Spermatogenesis refers to the differentiation of the spermatogonial stem cells (SSCs) located in the base seminiferous tubules into haploid spermatozoa. Prerequisites for in vitro spermatogenesis include an extracellular matrix (ECM), paracrine factors, and testicular somatic cells which play a supporting role for SSCs. Thus, the present study evaluated the potential of co-culturing Sertoli cells and SSCs embedded in a hybrid hydrogel of agarose and laminin, the main components of the ECM. Following the three–week conventional culture of human testicular cells, the cells were cultured in agarose hydrogel or agarose/laminin one (hybrid) for 74 days. Then, immunocytochemistry, real-time PCR, electron microscopy, and morphological staining methods were applied to analyze the presence of SSCs, as well as the other cells of the different stages of spermatogenesis. Based on the results, the colonies with positive spermatogenesis markers were observed in both culture systems. The existence of the cells of all three phases of spermatogenesis (spermatogonia, meiosis, and spermiogenesis) was confirmed in the two groups, while morphological spermatozoa were detected only in the hybrid hydrogel group. Finally, a biologically improved 3D matrix can support all the physiological activities of SSCs such as survival, proliferation, and differentiation.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2023.123801</identifier><identifier>PMID: 36842740</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Agarose ; Cell Differentiation - physiology ; coculture ; electron microscopy ; extracellular matrix ; haploidy ; Humans ; Hybrid hydrogel ; hydrogels ; Hydrogels - pharmacology ; immunocytochemistry ; In vitro spermatogenesis ; Laminin ; Laminin - pharmacology ; Male ; meiosis ; quantitative polymerase chain reaction ; Sepharose ; Spermatogenesis ; spermatogonia ; Spermatogonial stem cells ; Spermatozoa ; spermiogenesis ; Stem Cells ; testes</subject><ispartof>International journal of biological macromolecules, 2023-04, Vol.235, p.123801-123801, Article 123801</ispartof><rights>2023 Elsevier B.V.</rights><rights>Copyright © 2023 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-3f03b164f3c39d16f09d93f94fd28e96c1081d7dc1b959c24e15a558d263a5fc3</citedby><cites>FETCH-LOGICAL-c449t-3f03b164f3c39d16f09d93f94fd28e96c1081d7dc1b959c24e15a558d263a5fc3</cites><orcidid>0000-0002-4310-0381</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0141813023006955$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36842740$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jabari, Ayob</creatorcontrib><creatorcontrib>Gholami, Keykavos</creatorcontrib><creatorcontrib>Khadivi, Farnaz</creatorcontrib><creatorcontrib>Koruji, Morteza</creatorcontrib><creatorcontrib>Amidi, Fardin</creatorcontrib><creatorcontrib>Gilani, Mohammad Ali Sadighi</creatorcontrib><creatorcontrib>Mahabadi, Vahid Pirhajati</creatorcontrib><creatorcontrib>Nikmahzar, Aghbibi</creatorcontrib><creatorcontrib>Salem, Maryam</creatorcontrib><creatorcontrib>Movassagh, Sepideh Ashouri</creatorcontrib><creatorcontrib>Feizollahi, Narjes</creatorcontrib><creatorcontrib>Abbasi, Mehdi</creatorcontrib><title>In vitro complete differentiation of human spermatogonial stem cells to morphologic spermatozoa using a hybrid hydrogel of agarose and laminin</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Spermatogenesis refers to the differentiation of the spermatogonial stem cells (SSCs) located in the base seminiferous tubules into haploid spermatozoa. Prerequisites for in vitro spermatogenesis include an extracellular matrix (ECM), paracrine factors, and testicular somatic cells which play a supporting role for SSCs. Thus, the present study evaluated the potential of co-culturing Sertoli cells and SSCs embedded in a hybrid hydrogel of agarose and laminin, the main components of the ECM. Following the three–week conventional culture of human testicular cells, the cells were cultured in agarose hydrogel or agarose/laminin one (hybrid) for 74 days. Then, immunocytochemistry, real-time PCR, electron microscopy, and morphological staining methods were applied to analyze the presence of SSCs, as well as the other cells of the different stages of spermatogenesis. Based on the results, the colonies with positive spermatogenesis markers were observed in both culture systems. The existence of the cells of all three phases of spermatogenesis (spermatogonia, meiosis, and spermiogenesis) was confirmed in the two groups, while morphological spermatozoa were detected only in the hybrid hydrogel group. Finally, a biologically improved 3D matrix can support all the physiological activities of SSCs such as survival, proliferation, and differentiation.</description><subject>Agarose</subject><subject>Cell Differentiation - physiology</subject><subject>coculture</subject><subject>electron microscopy</subject><subject>extracellular matrix</subject><subject>haploidy</subject><subject>Humans</subject><subject>Hybrid hydrogel</subject><subject>hydrogels</subject><subject>Hydrogels - pharmacology</subject><subject>immunocytochemistry</subject><subject>In vitro spermatogenesis</subject><subject>Laminin</subject><subject>Laminin - pharmacology</subject><subject>Male</subject><subject>meiosis</subject><subject>quantitative polymerase chain reaction</subject><subject>Sepharose</subject><subject>Spermatogenesis</subject><subject>spermatogonia</subject><subject>Spermatogonial stem cells</subject><subject>Spermatozoa</subject><subject>spermiogenesis</subject><subject>Stem Cells</subject><subject>testes</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1r3DAQhkVpabZp_0LQsRdv9WVbvrWEfgQCubRnIUsjrxZLciU5kP6I_uZ42STXnF4Ynplh5kHoipI9JbT7ctz74-hT0GbPCON7yrgk9A3aUdkPDSGEv0U7QgVtJOXkAn0o5bhVu5bK9-iCd1KwXpAd-n8T8b2vOWGTwjJDBWy9c5AhVq-rTxEnhw9r0BGXBXLQNU0pej3jUiFgA_NccE04pLwc0pwmb17Af0njtfg4YY0PD2P2dgub0wTzaaqedE4FsI4Wzzr46ONH9M7pucCnp7xEf358_339q7m9-3lz_e22MUIMteGO8JF2wnHDB0s7RwY7cDcIZ5mEoTOUSGp7a-g4tINhAmir21Za1nHdOsMv0efz3CWnvyuUqoIvp1t0hLQWxSQXjDLa89fRXhIhezqQDe3OqNnuKhmcWrIPOj8oStRJmzqqZ23qpE2dtW2NV0871jGAfWl79rQBX88AbE-595BVMR6iAeszmKps8q_teAQhn67i</recordid><startdate>20230430</startdate><enddate>20230430</enddate><creator>Jabari, Ayob</creator><creator>Gholami, Keykavos</creator><creator>Khadivi, Farnaz</creator><creator>Koruji, Morteza</creator><creator>Amidi, Fardin</creator><creator>Gilani, Mohammad Ali Sadighi</creator><creator>Mahabadi, Vahid Pirhajati</creator><creator>Nikmahzar, Aghbibi</creator><creator>Salem, Maryam</creator><creator>Movassagh, Sepideh Ashouri</creator><creator>Feizollahi, Narjes</creator><creator>Abbasi, Mehdi</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-4310-0381</orcidid></search><sort><creationdate>20230430</creationdate><title>In vitro complete differentiation of human spermatogonial stem cells to morphologic spermatozoa using a hybrid hydrogel of agarose and laminin</title><author>Jabari, Ayob ; Gholami, Keykavos ; Khadivi, Farnaz ; Koruji, Morteza ; Amidi, Fardin ; Gilani, Mohammad Ali Sadighi ; Mahabadi, Vahid Pirhajati ; Nikmahzar, Aghbibi ; Salem, Maryam ; Movassagh, Sepideh Ashouri ; Feizollahi, Narjes ; Abbasi, Mehdi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-3f03b164f3c39d16f09d93f94fd28e96c1081d7dc1b959c24e15a558d263a5fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Agarose</topic><topic>Cell Differentiation - physiology</topic><topic>coculture</topic><topic>electron microscopy</topic><topic>extracellular matrix</topic><topic>haploidy</topic><topic>Humans</topic><topic>Hybrid hydrogel</topic><topic>hydrogels</topic><topic>Hydrogels - pharmacology</topic><topic>immunocytochemistry</topic><topic>In vitro spermatogenesis</topic><topic>Laminin</topic><topic>Laminin - pharmacology</topic><topic>Male</topic><topic>meiosis</topic><topic>quantitative polymerase chain reaction</topic><topic>Sepharose</topic><topic>Spermatogenesis</topic><topic>spermatogonia</topic><topic>Spermatogonial stem cells</topic><topic>Spermatozoa</topic><topic>spermiogenesis</topic><topic>Stem Cells</topic><topic>testes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jabari, Ayob</creatorcontrib><creatorcontrib>Gholami, Keykavos</creatorcontrib><creatorcontrib>Khadivi, Farnaz</creatorcontrib><creatorcontrib>Koruji, Morteza</creatorcontrib><creatorcontrib>Amidi, Fardin</creatorcontrib><creatorcontrib>Gilani, Mohammad Ali Sadighi</creatorcontrib><creatorcontrib>Mahabadi, Vahid Pirhajati</creatorcontrib><creatorcontrib>Nikmahzar, Aghbibi</creatorcontrib><creatorcontrib>Salem, Maryam</creatorcontrib><creatorcontrib>Movassagh, Sepideh Ashouri</creatorcontrib><creatorcontrib>Feizollahi, Narjes</creatorcontrib><creatorcontrib>Abbasi, Mehdi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jabari, Ayob</au><au>Gholami, Keykavos</au><au>Khadivi, Farnaz</au><au>Koruji, Morteza</au><au>Amidi, Fardin</au><au>Gilani, Mohammad Ali Sadighi</au><au>Mahabadi, Vahid Pirhajati</au><au>Nikmahzar, Aghbibi</au><au>Salem, Maryam</au><au>Movassagh, Sepideh Ashouri</au><au>Feizollahi, Narjes</au><au>Abbasi, Mehdi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro complete differentiation of human spermatogonial stem cells to morphologic spermatozoa using a hybrid hydrogel of agarose and laminin</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2023-04-30</date><risdate>2023</risdate><volume>235</volume><spage>123801</spage><epage>123801</epage><pages>123801-123801</pages><artnum>123801</artnum><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>Spermatogenesis refers to the differentiation of the spermatogonial stem cells (SSCs) located in the base seminiferous tubules into haploid spermatozoa. Prerequisites for in vitro spermatogenesis include an extracellular matrix (ECM), paracrine factors, and testicular somatic cells which play a supporting role for SSCs. Thus, the present study evaluated the potential of co-culturing Sertoli cells and SSCs embedded in a hybrid hydrogel of agarose and laminin, the main components of the ECM. Following the three–week conventional culture of human testicular cells, the cells were cultured in agarose hydrogel or agarose/laminin one (hybrid) for 74 days. Then, immunocytochemistry, real-time PCR, electron microscopy, and morphological staining methods were applied to analyze the presence of SSCs, as well as the other cells of the different stages of spermatogenesis. Based on the results, the colonies with positive spermatogenesis markers were observed in both culture systems. The existence of the cells of all three phases of spermatogenesis (spermatogonia, meiosis, and spermiogenesis) was confirmed in the two groups, while morphological spermatozoa were detected only in the hybrid hydrogel group. Finally, a biologically improved 3D matrix can support all the physiological activities of SSCs such as survival, proliferation, and differentiation.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>36842740</pmid><doi>10.1016/j.ijbiomac.2023.123801</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-4310-0381</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-8130 |
| ispartof | International journal of biological macromolecules, 2023-04, Vol.235, p.123801-123801, Article 123801 |
| issn | 0141-8130 1879-0003 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2780487190 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Agarose Cell Differentiation - physiology coculture electron microscopy extracellular matrix haploidy Humans Hybrid hydrogel hydrogels Hydrogels - pharmacology immunocytochemistry In vitro spermatogenesis Laminin Laminin - pharmacology Male meiosis quantitative polymerase chain reaction Sepharose Spermatogenesis spermatogonia Spermatogonial stem cells Spermatozoa spermiogenesis Stem Cells testes |
| title | In vitro complete differentiation of human spermatogonial stem cells to morphologic spermatozoa using a hybrid hydrogel of agarose and laminin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T07%3A04%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20vitro%20complete%20differentiation%20of%20human%20spermatogonial%20stem%20cells%20to%20morphologic%20spermatozoa%20using%20a%20hybrid%20hydrogel%20of%20agarose%20and%20laminin&rft.jtitle=International%20journal%20of%20biological%20macromolecules&rft.au=Jabari,%20Ayob&rft.date=2023-04-30&rft.volume=235&rft.spage=123801&rft.epage=123801&rft.pages=123801-123801&rft.artnum=123801&rft.issn=0141-8130&rft.eissn=1879-0003&rft_id=info:doi/10.1016/j.ijbiomac.2023.123801&rft_dat=%3Cproquest_cross%3E2834212173%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2780487190&rft_id=info:pmid/36842740&rft_els_id=S0141813023006955&rfr_iscdi=true |