The rapid high-throughput screening of ω-transaminases via a colorimetric method using aliphatic α-diketones as amino acceptors

ω-Transaminases (ω-TAs) are widely available for the production of chiral amines and unnatural amino acids. Herein, a rapid spectrophotometric method was developed for screening ω-TAs based on the colored products that can be generated from transamination reactions between aliphatic α-diketones and...

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Veröffentlicht in:Analytical and bioanalytical chemistry 2023-04, Vol.415 (9), p.1733-1740
Hauptverfasser: Tang, Kexin, Dong, Jiacheng, Zheng, Zhengheng, Zhang, Ting, Pan, Huayi, Jia, Honghua, Li, Yan, Wei, Ping
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Sprache:eng
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