Online micro solid phase extraction coupled with ultra‐performance liquid chromatography–tandem mass spectrometry for trace analysis of endogenous plant hormones in Ulva linza
Objective Ulva linza (L.) is a species of green algae widely distributed in China. We aimed to establish a sensitive online analytical method for quantification of endogenous phytohormones in fresh minute seaweed samples. Method The method for quantification of endogenous plant hormones in fresh min...
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| Veröffentlicht in: | Phytochemical analysis 2023-04, Vol.34 (3), p.363-371 |
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| creator | Li, Yuxuan Duan, Chunfeng Ning, Haijing Ni, Lanxiu Li, Jiamin Gao, Yan Ding, Kun Guan, Yafeng |
| description | Objective
Ulva linza (L.) is a species of green algae widely distributed in China. We aimed to establish a sensitive online analytical method for quantification of endogenous phytohormones in fresh minute seaweed samples.
Method
The method for quantification of endogenous plant hormones in fresh minute samples was developed based on a homemade online micro solid phase extraction (m‐SPE) system coupled with an ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC‐MS/MS) platform. The online m‐SPE instrument injected the eluent of m‐SPE directly onto the LC separation column, improving the utilization of samples and saving time. The m‐SPE column, of which the effective size was 9.6 × 2 mm i.d., was filled with 19 mg of C18 (10 μm).
Results
Under optimized conditions, the limits of detection were 0.002–0.060 ng ml−1 for five plant hormones. The actual sample recoveries of phytohormones were 76.4–103.4% and the coefficients of variance were below 14.1%. The temporal distribution of these endogenous plant hormones of U. linza during different growth periods is described.
Conclusion
The proposed online m‐SPE method was successfully applied to quantification of endogenous acidic and alkaline plant hormones in U. linza. It provides important information for the further study of the physiological and ecological effects of plant hormones in lower algal species.
A sensitive online analytical method was established for quantification of endogenous phytohormones in fresh minute seaweed samples based on a homemade online m‐SPE instrument coupled with UPLC‐MS/MS. The limits of detection were 0.002–0.060 ng mL−1 for five plant hormones. The actual sample recoveries of phytohormones were 76.4%–103.4%, and the coefficient of variance were below 14.1%. The method is used to determine the content of endogenous plant hormones in Ulva linza (L.) and its temporal distribution in different growing period. |
| doi_str_mv | 10.1002/pca.3215 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2780063730</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2780063730</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3495-9945f0dfb8fbb5dda7d09a3794590f1d5e415b6cecb1edc58650fdf071eb11b33</originalsourceid><addsrcrecordid>eNp1kc1qFTEUx4Mo9loFn0ACbrqZmtxMZibLcvELCnVh10MmOemkZJI0mbGOqz5CwUfxjfok5tqqILg6cM7v_M_HH6GXlBxTQrZvopLHbEv5I7ShRIiK8oY_RhsieFcRVtcH6FnOl4SUmmieogPWdFvWcrZBP868sx7wZFUKOAdnNY6jzIDh65ykmm3wWIUlOtD42s4jXlzJ393cRkgmpEl6BdjZq6U0qjGFSc7hIsk4rnc332fpNUx4kjnjHEHNpQ5zWnHpxHt5wNJLt2abcTAYvA4X4MOScXTSz3gsA4KHjK3H5-6LLIP8N_kcPTHSZXjxEA_R-bu3n3cfqtOz9x93J6eVYrXglRA1N0SboTPDwLWWrSZCsrakBTFUc6gpHxoFaqCgFe8aTow2pKUwUDowdoiO7nVjClcL5LmfbFbgympQduy3bUdIw1pGCvr6H_QyLKmctqcEE-X1LfkrWH6dcwLTx2Qnmdaekn5vZF-M7PdGFvTVg-AyTKD_gL-dK0B1D1xbB-t_hfpPu5Nfgj8BJOiuag</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2793900070</pqid></control><display><type>article</type><title>Online micro solid phase extraction coupled with ultra‐performance liquid chromatography–tandem mass spectrometry for trace analysis of endogenous plant hormones in Ulva linza</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Li, Yuxuan ; Duan, Chunfeng ; Ning, Haijing ; Ni, Lanxiu ; Li, Jiamin ; Gao, Yan ; Ding, Kun ; Guan, Yafeng</creator><creatorcontrib>Li, Yuxuan ; Duan, Chunfeng ; Ning, Haijing ; Ni, Lanxiu ; Li, Jiamin ; Gao, Yan ; Ding, Kun ; Guan, Yafeng</creatorcontrib><description>Objective
Ulva linza (L.) is a species of green algae widely distributed in China. We aimed to establish a sensitive online analytical method for quantification of endogenous phytohormones in fresh minute seaweed samples.
Method
The method for quantification of endogenous plant hormones in fresh minute samples was developed based on a homemade online micro solid phase extraction (m‐SPE) system coupled with an ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC‐MS/MS) platform. The online m‐SPE instrument injected the eluent of m‐SPE directly onto the LC separation column, improving the utilization of samples and saving time. The m‐SPE column, of which the effective size was 9.6 × 2 mm i.d., was filled with 19 mg of C18 (10 μm).
Results
Under optimized conditions, the limits of detection were 0.002–0.060 ng ml−1 for five plant hormones. The actual sample recoveries of phytohormones were 76.4–103.4% and the coefficients of variance were below 14.1%. The temporal distribution of these endogenous plant hormones of U. linza during different growth periods is described.
Conclusion
The proposed online m‐SPE method was successfully applied to quantification of endogenous acidic and alkaline plant hormones in U. linza. It provides important information for the further study of the physiological and ecological effects of plant hormones in lower algal species.
A sensitive online analytical method was established for quantification of endogenous phytohormones in fresh minute seaweed samples based on a homemade online m‐SPE instrument coupled with UPLC‐MS/MS. The limits of detection were 0.002–0.060 ng mL−1 for five plant hormones. The actual sample recoveries of phytohormones were 76.4%–103.4%, and the coefficient of variance were below 14.1%. The method is used to determine the content of endogenous plant hormones in Ulva linza (L.) and its temporal distribution in different growing period.</description><identifier>ISSN: 0958-0344</identifier><identifier>EISSN: 1099-1565</identifier><identifier>DOI: 10.1002/pca.3215</identifier><identifier>PMID: 36823753</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Algae ; Aquatic plants ; Chromatography ; Chromatography, High Pressure Liquid - methods ; Chromatography, Liquid - methods ; Ecological effects ; endogenous plant hormones ; Hormones ; Internet ; Liquid chromatography ; Mass spectrometry ; Mass spectroscopy ; online m‐SPE ; Physiological effects ; Phytohormones ; Plant Growth Regulators ; Plant hormones ; Scientific imaging ; Seaweeds ; Solid Phase Extraction - methods ; Solid phases ; Tandem Mass Spectrometry - methods ; Temporal distribution ; Ulva ; Ulva linza</subject><ispartof>Phytochemical analysis, 2023-04, Vol.34 (3), p.363-371</ispartof><rights>2023 John Wiley & Sons Ltd.</rights><rights>2023 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3495-9945f0dfb8fbb5dda7d09a3794590f1d5e415b6cecb1edc58650fdf071eb11b33</citedby><cites>FETCH-LOGICAL-c3495-9945f0dfb8fbb5dda7d09a3794590f1d5e415b6cecb1edc58650fdf071eb11b33</cites><orcidid>0000-0002-5424-927X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpca.3215$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpca.3215$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36823753$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Yuxuan</creatorcontrib><creatorcontrib>Duan, Chunfeng</creatorcontrib><creatorcontrib>Ning, Haijing</creatorcontrib><creatorcontrib>Ni, Lanxiu</creatorcontrib><creatorcontrib>Li, Jiamin</creatorcontrib><creatorcontrib>Gao, Yan</creatorcontrib><creatorcontrib>Ding, Kun</creatorcontrib><creatorcontrib>Guan, Yafeng</creatorcontrib><title>Online micro solid phase extraction coupled with ultra‐performance liquid chromatography–tandem mass spectrometry for trace analysis of endogenous plant hormones in Ulva linza</title><title>Phytochemical analysis</title><addtitle>Phytochem Anal</addtitle><description>Objective
Ulva linza (L.) is a species of green algae widely distributed in China. We aimed to establish a sensitive online analytical method for quantification of endogenous phytohormones in fresh minute seaweed samples.
Method
The method for quantification of endogenous plant hormones in fresh minute samples was developed based on a homemade online micro solid phase extraction (m‐SPE) system coupled with an ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC‐MS/MS) platform. The online m‐SPE instrument injected the eluent of m‐SPE directly onto the LC separation column, improving the utilization of samples and saving time. The m‐SPE column, of which the effective size was 9.6 × 2 mm i.d., was filled with 19 mg of C18 (10 μm).
Results
Under optimized conditions, the limits of detection were 0.002–0.060 ng ml−1 for five plant hormones. The actual sample recoveries of phytohormones were 76.4–103.4% and the coefficients of variance were below 14.1%. The temporal distribution of these endogenous plant hormones of U. linza during different growth periods is described.
Conclusion
The proposed online m‐SPE method was successfully applied to quantification of endogenous acidic and alkaline plant hormones in U. linza. It provides important information for the further study of the physiological and ecological effects of plant hormones in lower algal species.
A sensitive online analytical method was established for quantification of endogenous phytohormones in fresh minute seaweed samples based on a homemade online m‐SPE instrument coupled with UPLC‐MS/MS. The limits of detection were 0.002–0.060 ng mL−1 for five plant hormones. The actual sample recoveries of phytohormones were 76.4%–103.4%, and the coefficient of variance were below 14.1%. The method is used to determine the content of endogenous plant hormones in Ulva linza (L.) and its temporal distribution in different growing period.</description><subject>Algae</subject><subject>Aquatic plants</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Chromatography, Liquid - methods</subject><subject>Ecological effects</subject><subject>endogenous plant hormones</subject><subject>Hormones</subject><subject>Internet</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>online m‐SPE</subject><subject>Physiological effects</subject><subject>Phytohormones</subject><subject>Plant Growth Regulators</subject><subject>Plant hormones</subject><subject>Scientific imaging</subject><subject>Seaweeds</subject><subject>Solid Phase Extraction - methods</subject><subject>Solid phases</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Temporal distribution</subject><subject>Ulva</subject><subject>Ulva linza</subject><issn>0958-0344</issn><issn>1099-1565</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1qFTEUx4Mo9loFn0ACbrqZmtxMZibLcvELCnVh10MmOemkZJI0mbGOqz5CwUfxjfok5tqqILg6cM7v_M_HH6GXlBxTQrZvopLHbEv5I7ShRIiK8oY_RhsieFcRVtcH6FnOl4SUmmieogPWdFvWcrZBP868sx7wZFUKOAdnNY6jzIDh65ykmm3wWIUlOtD42s4jXlzJ393cRkgmpEl6BdjZq6U0qjGFSc7hIsk4rnc332fpNUx4kjnjHEHNpQ5zWnHpxHt5wNJLt2abcTAYvA4X4MOScXTSz3gsA4KHjK3H5-6LLIP8N_kcPTHSZXjxEA_R-bu3n3cfqtOz9x93J6eVYrXglRA1N0SboTPDwLWWrSZCsrakBTFUc6gpHxoFaqCgFe8aTow2pKUwUDowdoiO7nVjClcL5LmfbFbgympQduy3bUdIw1pGCvr6H_QyLKmctqcEE-X1LfkrWH6dcwLTx2Qnmdaekn5vZF-M7PdGFvTVg-AyTKD_gL-dK0B1D1xbB-t_hfpPu5Nfgj8BJOiuag</recordid><startdate>202304</startdate><enddate>202304</enddate><creator>Li, Yuxuan</creator><creator>Duan, Chunfeng</creator><creator>Ning, Haijing</creator><creator>Ni, Lanxiu</creator><creator>Li, Jiamin</creator><creator>Gao, Yan</creator><creator>Ding, Kun</creator><creator>Guan, Yafeng</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5424-927X</orcidid></search><sort><creationdate>202304</creationdate><title>Online micro solid phase extraction coupled with ultra‐performance liquid chromatography–tandem mass spectrometry for trace analysis of endogenous plant hormones in Ulva linza</title><author>Li, Yuxuan ; Duan, Chunfeng ; Ning, Haijing ; Ni, Lanxiu ; Li, Jiamin ; Gao, Yan ; Ding, Kun ; Guan, Yafeng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3495-9945f0dfb8fbb5dda7d09a3794590f1d5e415b6cecb1edc58650fdf071eb11b33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Algae</topic><topic>Aquatic plants</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Chromatography, Liquid - methods</topic><topic>Ecological effects</topic><topic>endogenous plant hormones</topic><topic>Hormones</topic><topic>Internet</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>online m‐SPE</topic><topic>Physiological effects</topic><topic>Phytohormones</topic><topic>Plant Growth Regulators</topic><topic>Plant hormones</topic><topic>Scientific imaging</topic><topic>Seaweeds</topic><topic>Solid Phase Extraction - methods</topic><topic>Solid phases</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Temporal distribution</topic><topic>Ulva</topic><topic>Ulva linza</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Yuxuan</creatorcontrib><creatorcontrib>Duan, Chunfeng</creatorcontrib><creatorcontrib>Ning, Haijing</creatorcontrib><creatorcontrib>Ni, Lanxiu</creatorcontrib><creatorcontrib>Li, Jiamin</creatorcontrib><creatorcontrib>Gao, Yan</creatorcontrib><creatorcontrib>Ding, Kun</creatorcontrib><creatorcontrib>Guan, Yafeng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Phytochemical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Yuxuan</au><au>Duan, Chunfeng</au><au>Ning, Haijing</au><au>Ni, Lanxiu</au><au>Li, Jiamin</au><au>Gao, Yan</au><au>Ding, Kun</au><au>Guan, Yafeng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Online micro solid phase extraction coupled with ultra‐performance liquid chromatography–tandem mass spectrometry for trace analysis of endogenous plant hormones in Ulva linza</atitle><jtitle>Phytochemical analysis</jtitle><addtitle>Phytochem Anal</addtitle><date>2023-04</date><risdate>2023</risdate><volume>34</volume><issue>3</issue><spage>363</spage><epage>371</epage><pages>363-371</pages><issn>0958-0344</issn><eissn>1099-1565</eissn><abstract>Objective
Ulva linza (L.) is a species of green algae widely distributed in China. We aimed to establish a sensitive online analytical method for quantification of endogenous phytohormones in fresh minute seaweed samples.
Method
The method for quantification of endogenous plant hormones in fresh minute samples was developed based on a homemade online micro solid phase extraction (m‐SPE) system coupled with an ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC‐MS/MS) platform. The online m‐SPE instrument injected the eluent of m‐SPE directly onto the LC separation column, improving the utilization of samples and saving time. The m‐SPE column, of which the effective size was 9.6 × 2 mm i.d., was filled with 19 mg of C18 (10 μm).
Results
Under optimized conditions, the limits of detection were 0.002–0.060 ng ml−1 for five plant hormones. The actual sample recoveries of phytohormones were 76.4–103.4% and the coefficients of variance were below 14.1%. The temporal distribution of these endogenous plant hormones of U. linza during different growth periods is described.
Conclusion
The proposed online m‐SPE method was successfully applied to quantification of endogenous acidic and alkaline plant hormones in U. linza. It provides important information for the further study of the physiological and ecological effects of plant hormones in lower algal species.
A sensitive online analytical method was established for quantification of endogenous phytohormones in fresh minute seaweed samples based on a homemade online m‐SPE instrument coupled with UPLC‐MS/MS. The limits of detection were 0.002–0.060 ng mL−1 for five plant hormones. The actual sample recoveries of phytohormones were 76.4%–103.4%, and the coefficient of variance were below 14.1%. The method is used to determine the content of endogenous plant hormones in Ulva linza (L.) and its temporal distribution in different growing period.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>36823753</pmid><doi>10.1002/pca.3215</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-5424-927X</orcidid></addata></record> |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Algae Aquatic plants Chromatography Chromatography, High Pressure Liquid - methods Chromatography, Liquid - methods Ecological effects endogenous plant hormones Hormones Internet Liquid chromatography Mass spectrometry Mass spectroscopy online m‐SPE Physiological effects Phytohormones Plant Growth Regulators Plant hormones Scientific imaging Seaweeds Solid Phase Extraction - methods Solid phases Tandem Mass Spectrometry - methods Temporal distribution Ulva Ulva linza |
| title | Online micro solid phase extraction coupled with ultra‐performance liquid chromatography–tandem mass spectrometry for trace analysis of endogenous plant hormones in Ulva linza |
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