Indirect co‐culture of islet cells in 3D biocompatible collagen/laminin scaffold with angiomiRs transfected mesenchymal stem cells
Diabetes is an autoimmune disease in which the pancreatic islets produce insufficient insulin. One of the treatment strategies is islet isolation, which may damage these cells as they lack vasculature. Biocompatible scaffolds are one of the efficient techniques for dealing with this issue. The curre...
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| Veröffentlicht in: | Cell biochemistry and function 2023-04, Vol.41 (3), p.296-308 |
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| creator | Sabet Sarvestani, Fatemeh Tamaddon, Ali‐Mohammad Yaghoobi, Ramin Geramizadeh, Bita Abolmaali, Samira Sadat Kaviani, Maryam Keshtkar, Somayeh Pakbaz, Sara Azarpira, Negar |
| description | Diabetes is an autoimmune disease in which the pancreatic islets produce insufficient insulin. One of the treatment strategies is islet isolation, which may damage these cells as they lack vasculature. Biocompatible scaffolds are one of the efficient techniques for dealing with this issue. The current study is aimed to determine the effect of transfected BM‐MSCS with angiomiR‐126 and ‐210 on the survival and functionality of islets loaded into a 3D scaffold via laminin (LMN). AngiomiRs/Poly Ethylenimine polyplexes were transfected into bone marrow‐mesenchymal stem cells (BM‐MSCs), followed by 3‐day indirect co‐culturing with islets laden in collagen (Col)‐based hydrogel scaffolds containing LMN. Islet proliferation and viability were significantly increased in LMN‐containing scaffolds, particularly in the miRNA‐126 treated group. Insulin gene expression was superior in Col scaffolds, especially, in the BM‐MSCs/miRNA‐126 treated group. VEGF was upregulated in the LMN‐containing scaffolds in both miRNA‐treated groups, specifically in the miRNA‐210, leading to VEGF secretion. MiRNAs' target genes showed no downregulation in LMN‐free scaffolds; while a drastic downregulation was seen in the LMN‐containing scaffolds. The highest insulin secretion was recorded in the Oxidized dextran (Odex)/ColLMN+ group with miRNA‐126. LMN‐containing biocompatible scaffolds, once combined with angiomiRs and their downstream effectors, promote islets survival and restore function, leading to enhanced angiogenesis and glycemic status.
Significance statement
This study investigated the synergy effect of LMN and angiomiRs on islet cells. It was shown that LMN‐containing scaffolds, once combined with miRNAs‐126 and ‐210 might help islets survive and restore function, thereby enhancing angiogenesis and glycemic status. These data could help to improve islets transplantation state in diabetic conditions. |
| doi_str_mv | 10.1002/cbf.3781 |
| format | Article |
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Significance statement
This study investigated the synergy effect of LMN and angiomiRs on islet cells. It was shown that LMN‐containing scaffolds, once combined with miRNAs‐126 and ‐210 might help islets survive and restore function, thereby enhancing angiogenesis and glycemic status. These data could help to improve islets transplantation state in diabetic conditions.</description><identifier>ISSN: 0263-6484</identifier><identifier>EISSN: 1099-0844</identifier><identifier>DOI: 10.1002/cbf.3781</identifier><identifier>PMID: 36815688</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Angiogenesis ; Autoimmune diseases ; Biocompatibility ; Bone marrow ; bone marrow mesenchymal stem cells ; Cell culture ; Coculture Techniques ; Collagen ; Collagen - metabolism ; Dextran ; Dextrans ; Diabetes mellitus ; Gene expression ; Hydrogels ; Insulin ; Insulin - metabolism ; Insulin secretion ; Islet cells ; Islets of Langerhans ; Laminin ; Laminin - metabolism ; Laminin - pharmacology ; Mesenchymal stem cells ; Mesenchymal Stem Cells - metabolism ; MicroRNAs ; MicroRNAs - metabolism ; miRNA ; pancreatic islet ; Recovery of function ; Ribonucleic acid ; RNA ; Scaffolds ; Secretion ; Stem cells ; Survival ; Tissue Scaffolds ; Transplantation ; Vascular endothelial growth factor ; Vascular Endothelial Growth Factor A - genetics ; Vascular Endothelial Growth Factor A - metabolism</subject><ispartof>Cell biochemistry and function, 2023-04, Vol.41 (3), p.296-308</ispartof><rights>2023 John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3491-aa3c2e98abb5dec8bda8c2d5f30ee08d4f90ceb17616e728dcba2d35901236183</citedby><cites>FETCH-LOGICAL-c3491-aa3c2e98abb5dec8bda8c2d5f30ee08d4f90ceb17616e728dcba2d35901236183</cites><orcidid>0000-0002-5737-0323 ; 0000-0002-1518-1583</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbf.3781$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbf.3781$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36815688$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sabet Sarvestani, Fatemeh</creatorcontrib><creatorcontrib>Tamaddon, Ali‐Mohammad</creatorcontrib><creatorcontrib>Yaghoobi, Ramin</creatorcontrib><creatorcontrib>Geramizadeh, Bita</creatorcontrib><creatorcontrib>Abolmaali, Samira Sadat</creatorcontrib><creatorcontrib>Kaviani, Maryam</creatorcontrib><creatorcontrib>Keshtkar, Somayeh</creatorcontrib><creatorcontrib>Pakbaz, Sara</creatorcontrib><creatorcontrib>Azarpira, Negar</creatorcontrib><title>Indirect co‐culture of islet cells in 3D biocompatible collagen/laminin scaffold with angiomiRs transfected mesenchymal stem cells</title><title>Cell biochemistry and function</title><addtitle>Cell Biochem Funct</addtitle><description>Diabetes is an autoimmune disease in which the pancreatic islets produce insufficient insulin. One of the treatment strategies is islet isolation, which may damage these cells as they lack vasculature. Biocompatible scaffolds are one of the efficient techniques for dealing with this issue. The current study is aimed to determine the effect of transfected BM‐MSCS with angiomiR‐126 and ‐210 on the survival and functionality of islets loaded into a 3D scaffold via laminin (LMN). AngiomiRs/Poly Ethylenimine polyplexes were transfected into bone marrow‐mesenchymal stem cells (BM‐MSCs), followed by 3‐day indirect co‐culturing with islets laden in collagen (Col)‐based hydrogel scaffolds containing LMN. Islet proliferation and viability were significantly increased in LMN‐containing scaffolds, particularly in the miRNA‐126 treated group. Insulin gene expression was superior in Col scaffolds, especially, in the BM‐MSCs/miRNA‐126 treated group. VEGF was upregulated in the LMN‐containing scaffolds in both miRNA‐treated groups, specifically in the miRNA‐210, leading to VEGF secretion. MiRNAs' target genes showed no downregulation in LMN‐free scaffolds; while a drastic downregulation was seen in the LMN‐containing scaffolds. The highest insulin secretion was recorded in the Oxidized dextran (Odex)/ColLMN+ group with miRNA‐126. LMN‐containing biocompatible scaffolds, once combined with angiomiRs and their downstream effectors, promote islets survival and restore function, leading to enhanced angiogenesis and glycemic status.
Significance statement
This study investigated the synergy effect of LMN and angiomiRs on islet cells. It was shown that LMN‐containing scaffolds, once combined with miRNAs‐126 and ‐210 might help islets survive and restore function, thereby enhancing angiogenesis and glycemic status. These data could help to improve islets transplantation state in diabetic conditions.</description><subject>Angiogenesis</subject><subject>Autoimmune diseases</subject><subject>Biocompatibility</subject><subject>Bone marrow</subject><subject>bone marrow mesenchymal stem cells</subject><subject>Cell culture</subject><subject>Coculture Techniques</subject><subject>Collagen</subject><subject>Collagen - metabolism</subject><subject>Dextran</subject><subject>Dextrans</subject><subject>Diabetes mellitus</subject><subject>Gene expression</subject><subject>Hydrogels</subject><subject>Insulin</subject><subject>Insulin - metabolism</subject><subject>Insulin secretion</subject><subject>Islet cells</subject><subject>Islets of Langerhans</subject><subject>Laminin</subject><subject>Laminin - metabolism</subject><subject>Laminin - pharmacology</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stem Cells - metabolism</subject><subject>MicroRNAs</subject><subject>MicroRNAs - metabolism</subject><subject>miRNA</subject><subject>pancreatic islet</subject><subject>Recovery of function</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Scaffolds</subject><subject>Secretion</subject><subject>Stem cells</subject><subject>Survival</subject><subject>Tissue Scaffolds</subject><subject>Transplantation</subject><subject>Vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><issn>0263-6484</issn><issn>1099-0844</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctqFEEUQAsxmMko-AVS4MZNJ_XoR_VSR6OBQEB03dTjVlKhHmNVN2F2WfgBfqNfkhonigiuLlwO5144CL2k5JQSws60sqd8EPQJWlEyjg0RbfsUrQjredO3oj1GJ6XcEkLGnpNn6Jj3gna9ECv0_SIal0HPWKef9z_04uclA04Wu-KhbsH7gl3E_D1WLukUtnJ2ykPlvZfXEM-8DC5WomhpbfIG37n5Bst47VJwnwues4zF1hNgcIACUd_sgvS4zBAO_ufoyEpf4MXjXKOv5x--bD41l1cfLzZvLxvN25E2UnLNYBRSqc6AFspIoZnpLCcARJjWjkSDokNPexiYMFpJZng3Esp4TwVfozcH7zanbwuUeQqu7D-QEdJSJjYMI--YqPgavf4HvU1LjvW7So2iKtvuL6HOqZQMdtpmF2TeTZRM-zJTLTPty1T01aNwUQHMH_B3igo0B-DOedj9VzRt3p3_Ej4An7maQg</recordid><startdate>202304</startdate><enddate>202304</enddate><creator>Sabet Sarvestani, Fatemeh</creator><creator>Tamaddon, Ali‐Mohammad</creator><creator>Yaghoobi, Ramin</creator><creator>Geramizadeh, Bita</creator><creator>Abolmaali, Samira Sadat</creator><creator>Kaviani, Maryam</creator><creator>Keshtkar, Somayeh</creator><creator>Pakbaz, Sara</creator><creator>Azarpira, Negar</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5737-0323</orcidid><orcidid>https://orcid.org/0000-0002-1518-1583</orcidid></search><sort><creationdate>202304</creationdate><title>Indirect co‐culture of islet cells in 3D biocompatible collagen/laminin scaffold with angiomiRs transfected mesenchymal stem cells</title><author>Sabet Sarvestani, Fatemeh ; 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One of the treatment strategies is islet isolation, which may damage these cells as they lack vasculature. Biocompatible scaffolds are one of the efficient techniques for dealing with this issue. The current study is aimed to determine the effect of transfected BM‐MSCS with angiomiR‐126 and ‐210 on the survival and functionality of islets loaded into a 3D scaffold via laminin (LMN). AngiomiRs/Poly Ethylenimine polyplexes were transfected into bone marrow‐mesenchymal stem cells (BM‐MSCs), followed by 3‐day indirect co‐culturing with islets laden in collagen (Col)‐based hydrogel scaffolds containing LMN. Islet proliferation and viability were significantly increased in LMN‐containing scaffolds, particularly in the miRNA‐126 treated group. Insulin gene expression was superior in Col scaffolds, especially, in the BM‐MSCs/miRNA‐126 treated group. VEGF was upregulated in the LMN‐containing scaffolds in both miRNA‐treated groups, specifically in the miRNA‐210, leading to VEGF secretion. MiRNAs' target genes showed no downregulation in LMN‐free scaffolds; while a drastic downregulation was seen in the LMN‐containing scaffolds. The highest insulin secretion was recorded in the Oxidized dextran (Odex)/ColLMN+ group with miRNA‐126. LMN‐containing biocompatible scaffolds, once combined with angiomiRs and their downstream effectors, promote islets survival and restore function, leading to enhanced angiogenesis and glycemic status.
Significance statement
This study investigated the synergy effect of LMN and angiomiRs on islet cells. It was shown that LMN‐containing scaffolds, once combined with miRNAs‐126 and ‐210 might help islets survive and restore function, thereby enhancing angiogenesis and glycemic status. These data could help to improve islets transplantation state in diabetic conditions.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>36815688</pmid><doi>10.1002/cbf.3781</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-5737-0323</orcidid><orcidid>https://orcid.org/0000-0002-1518-1583</orcidid></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Angiogenesis Autoimmune diseases Biocompatibility Bone marrow bone marrow mesenchymal stem cells Cell culture Coculture Techniques Collagen Collagen - metabolism Dextran Dextrans Diabetes mellitus Gene expression Hydrogels Insulin Insulin - metabolism Insulin secretion Islet cells Islets of Langerhans Laminin Laminin - metabolism Laminin - pharmacology Mesenchymal stem cells Mesenchymal Stem Cells - metabolism MicroRNAs MicroRNAs - metabolism miRNA pancreatic islet Recovery of function Ribonucleic acid RNA Scaffolds Secretion Stem cells Survival Tissue Scaffolds Transplantation Vascular endothelial growth factor Vascular Endothelial Growth Factor A - genetics Vascular Endothelial Growth Factor A - metabolism |
| title | Indirect co‐culture of islet cells in 3D biocompatible collagen/laminin scaffold with angiomiRs transfected mesenchymal stem cells |
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