Polyampholyte‐Based Polymer Hydrogels for the Long‐Term Storage, Protection and Delivery of Therapeutic Proteins
Protein storage and delivery are crucial for biomedical applications such as protein therapeutics and recombinant proteins. Lack of proper protocols results in the denaturation of proteins, rendering them inactive and manifesting undesired side effects. In this study, polyampholyte‐based (succinylat...
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| Veröffentlicht in: | Advanced healthcare materials 2023-07, Vol.12 (17), p.e2203253-n/a |
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| creator | Rajan, Robin Kumar, Nishant Zhao, Dandan Dai, Xianda Kawamoto, Keiko Matsumura, Kazuaki |
| description | Protein storage and delivery are crucial for biomedical applications such as protein therapeutics and recombinant proteins. Lack of proper protocols results in the denaturation of proteins, rendering them inactive and manifesting undesired side effects. In this study, polyampholyte‐based (succinylated ε‐poly‐l‐lysine) hydrogels containing polyvinyl alcohol and polyethylene glycol polymer matrices to stabilize proteins are developed. These hydrogels facilitated the loading and release of therapeutic amounts of proteins and withstood thermal and freezing stress (15 freeze–thaw cycles and temperatures of −80 °C and 37 °C), without resulting in protein denaturation and aggregation. To the best of our knowledge, this strategy has not been applied to the design of hydrogels constituting polymers, (in particular, polyampholyte‐based polymers) which have inherent efficiency to stabilize proteins and protect them from denaturation. Our findings can open up new avenues in protein biopharmaceutics for the design of materials that can store therapeutic proteins long‐term under severe stress and safely deliver them.
Polyampholyte‐based hydrogels are prepared using protein‐stabilizing polymers to encapsulate and release therapeutic proteins. The hydrogels ameliorate thermal and freezing stress‐induced denaturation and facilitate the retention of their native higher‐order structure and functions. This promotes a new dimension in therapeutic protein storage and delivery. |
| doi_str_mv | 10.1002/adhm.202203253 |
| format | Article |
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Polyampholyte‐based hydrogels are prepared using protein‐stabilizing polymers to encapsulate and release therapeutic proteins. The hydrogels ameliorate thermal and freezing stress‐induced denaturation and facilitate the retention of their native higher‐order structure and functions. This promotes a new dimension in therapeutic protein storage and delivery.</description><identifier>ISSN: 2192-2640</identifier><identifier>EISSN: 2192-2659</identifier><identifier>DOI: 10.1002/adhm.202203253</identifier><identifier>PMID: 36815203</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Biomedical materials ; Biopolymer denaturation ; Freeze thaw cycles ; Freeze-thawing ; hydrogel ; Hydrogels ; ice recrystallization inhibition ; Lysine ; Polyampholytes ; Polyethylene glycol ; Polymers ; Polyvinyl alcohol ; Protein denaturation ; protein therapeutics ; Proteins ; Side effects</subject><ispartof>Advanced healthcare materials, 2023-07, Vol.12 (17), p.e2203253-n/a</ispartof><rights>2023 Wiley‐VCH GmbH</rights><rights>2023 Wiley-VCH GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4393-8826e9f5a2dbc9e21534c49c415ef919548debe29c05ae12957b33544caa71913</citedby><cites>FETCH-LOGICAL-c4393-8826e9f5a2dbc9e21534c49c415ef919548debe29c05ae12957b33544caa71913</cites><orcidid>0000-0001-9484-3073 ; 0000-0002-6610-9661</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fadhm.202203253$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fadhm.202203253$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36815203$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rajan, Robin</creatorcontrib><creatorcontrib>Kumar, Nishant</creatorcontrib><creatorcontrib>Zhao, Dandan</creatorcontrib><creatorcontrib>Dai, Xianda</creatorcontrib><creatorcontrib>Kawamoto, Keiko</creatorcontrib><creatorcontrib>Matsumura, Kazuaki</creatorcontrib><title>Polyampholyte‐Based Polymer Hydrogels for the Long‐Term Storage, Protection and Delivery of Therapeutic Proteins</title><title>Advanced healthcare materials</title><addtitle>Adv Healthc Mater</addtitle><description>Protein storage and delivery are crucial for biomedical applications such as protein therapeutics and recombinant proteins. Lack of proper protocols results in the denaturation of proteins, rendering them inactive and manifesting undesired side effects. In this study, polyampholyte‐based (succinylated ε‐poly‐l‐lysine) hydrogels containing polyvinyl alcohol and polyethylene glycol polymer matrices to stabilize proteins are developed. These hydrogels facilitated the loading and release of therapeutic amounts of proteins and withstood thermal and freezing stress (15 freeze–thaw cycles and temperatures of −80 °C and 37 °C), without resulting in protein denaturation and aggregation. To the best of our knowledge, this strategy has not been applied to the design of hydrogels constituting polymers, (in particular, polyampholyte‐based polymers) which have inherent efficiency to stabilize proteins and protect them from denaturation. Our findings can open up new avenues in protein biopharmaceutics for the design of materials that can store therapeutic proteins long‐term under severe stress and safely deliver them.
Polyampholyte‐based hydrogels are prepared using protein‐stabilizing polymers to encapsulate and release therapeutic proteins. The hydrogels ameliorate thermal and freezing stress‐induced denaturation and facilitate the retention of their native higher‐order structure and functions. This promotes a new dimension in therapeutic protein storage and delivery.</description><subject>Biomedical materials</subject><subject>Biopolymer denaturation</subject><subject>Freeze thaw cycles</subject><subject>Freeze-thawing</subject><subject>hydrogel</subject><subject>Hydrogels</subject><subject>ice recrystallization inhibition</subject><subject>Lysine</subject><subject>Polyampholytes</subject><subject>Polyethylene glycol</subject><subject>Polymers</subject><subject>Polyvinyl alcohol</subject><subject>Protein denaturation</subject><subject>protein therapeutics</subject><subject>Proteins</subject><subject>Side effects</subject><issn>2192-2640</issn><issn>2192-2659</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNqFkc1OGzEURi1EBShlyxJZYsOCpPa1nYyX_LWplAokwtpyPHeSQTPjYM-AZscj9Bn7JHUUGiQ2vZvvyjr3yNJHyAlnI84YfLP5qh4BA2AClNgjR8A1DGGs9P5ul-yQHMf4xNKMFR9n_IAcihQqXR2R9t5Xva3XqxQt_nn7fWUj5nTzWmOg0z4PfolVpIUPtF0hnflmmbA5hpo-tD7YJV7Q--BbdG3pG2qbnN5gVb5g6Kkv6HyFwa6xa0u3xcomfiVfCltFPH7PAXn8fju_ng5ndz9-Xl_Ohk4KLYZZBmPUhbKQL5xG4EpIJ7WTXGGhuVYyy3GBoB1TFjloNVkIoaR01k645mJAzrfedfDPHcbW1GV0WFW2Qd9FA5OJFgok0wk9-4Q--S406XcGsiTlHFIOyGhLueBjDFiYdShrG3rDmdlUYjaVmF0l6eD0Xdstasx3-L8CEqC3wGtZYf8fnbm8mf76kP8F2TKZnQ</recordid><startdate>20230701</startdate><enddate>20230701</enddate><creator>Rajan, Robin</creator><creator>Kumar, Nishant</creator><creator>Zhao, Dandan</creator><creator>Dai, Xianda</creator><creator>Kawamoto, Keiko</creator><creator>Matsumura, Kazuaki</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T5</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7TO</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9484-3073</orcidid><orcidid>https://orcid.org/0000-0002-6610-9661</orcidid></search><sort><creationdate>20230701</creationdate><title>Polyampholyte‐Based Polymer Hydrogels for the Long‐Term Storage, Protection and Delivery of Therapeutic Proteins</title><author>Rajan, Robin ; Kumar, Nishant ; Zhao, Dandan ; Dai, Xianda ; Kawamoto, Keiko ; Matsumura, Kazuaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4393-8826e9f5a2dbc9e21534c49c415ef919548debe29c05ae12957b33544caa71913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Biomedical materials</topic><topic>Biopolymer denaturation</topic><topic>Freeze thaw cycles</topic><topic>Freeze-thawing</topic><topic>hydrogel</topic><topic>Hydrogels</topic><topic>ice recrystallization inhibition</topic><topic>Lysine</topic><topic>Polyampholytes</topic><topic>Polyethylene glycol</topic><topic>Polymers</topic><topic>Polyvinyl alcohol</topic><topic>Protein denaturation</topic><topic>protein therapeutics</topic><topic>Proteins</topic><topic>Side effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rajan, Robin</creatorcontrib><creatorcontrib>Kumar, Nishant</creatorcontrib><creatorcontrib>Zhao, Dandan</creatorcontrib><creatorcontrib>Dai, Xianda</creatorcontrib><creatorcontrib>Kawamoto, Keiko</creatorcontrib><creatorcontrib>Matsumura, Kazuaki</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Immunology Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Advanced healthcare materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rajan, Robin</au><au>Kumar, Nishant</au><au>Zhao, Dandan</au><au>Dai, Xianda</au><au>Kawamoto, Keiko</au><au>Matsumura, Kazuaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polyampholyte‐Based Polymer Hydrogels for the Long‐Term Storage, Protection and Delivery of Therapeutic Proteins</atitle><jtitle>Advanced healthcare materials</jtitle><addtitle>Adv Healthc Mater</addtitle><date>2023-07-01</date><risdate>2023</risdate><volume>12</volume><issue>17</issue><spage>e2203253</spage><epage>n/a</epage><pages>e2203253-n/a</pages><issn>2192-2640</issn><eissn>2192-2659</eissn><abstract>Protein storage and delivery are crucial for biomedical applications such as protein therapeutics and recombinant proteins. Lack of proper protocols results in the denaturation of proteins, rendering them inactive and manifesting undesired side effects. In this study, polyampholyte‐based (succinylated ε‐poly‐l‐lysine) hydrogels containing polyvinyl alcohol and polyethylene glycol polymer matrices to stabilize proteins are developed. These hydrogels facilitated the loading and release of therapeutic amounts of proteins and withstood thermal and freezing stress (15 freeze–thaw cycles and temperatures of −80 °C and 37 °C), without resulting in protein denaturation and aggregation. To the best of our knowledge, this strategy has not been applied to the design of hydrogels constituting polymers, (in particular, polyampholyte‐based polymers) which have inherent efficiency to stabilize proteins and protect them from denaturation. Our findings can open up new avenues in protein biopharmaceutics for the design of materials that can store therapeutic proteins long‐term under severe stress and safely deliver them.
Polyampholyte‐based hydrogels are prepared using protein‐stabilizing polymers to encapsulate and release therapeutic proteins. The hydrogels ameliorate thermal and freezing stress‐induced denaturation and facilitate the retention of their native higher‐order structure and functions. This promotes a new dimension in therapeutic protein storage and delivery.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>36815203</pmid><doi>10.1002/adhm.202203253</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-9484-3073</orcidid><orcidid>https://orcid.org/0000-0002-6610-9661</orcidid></addata></record> |
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| subjects | Biomedical materials Biopolymer denaturation Freeze thaw cycles Freeze-thawing hydrogel Hydrogels ice recrystallization inhibition Lysine Polyampholytes Polyethylene glycol Polymers Polyvinyl alcohol Protein denaturation protein therapeutics Proteins Side effects |
| title | Polyampholyte‐Based Polymer Hydrogels for the Long‐Term Storage, Protection and Delivery of Therapeutic Proteins |
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