Wound healing by transplantation of mesenchymal stromal cells loaded on polyethylene terephthalate scaffold: Implications for skin injury treatment

•Cutaneous wound healing continues being a serious medical problem.•Cell therapy based on mesenchymal stromal cells (MSC) has emerged as a promising therapeutic alternative to treat skin wounds.•Here we show that MSCs loaded on polyethylene terephthalate (PET) scaffold induce a rapid re-epithelializ...

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Veröffentlicht in:Injury 2023-04, Vol.54 (4), p.1071-1081
Hauptverfasser: Pereira, Betzabeth, Duque, Kharelys, Ramos-Gonzalez, Giselle, Díaz-Solano, Dylana, Wittig, Olga, Zamora, Mariela, Gledhill, Teresa, Cardier, José E.
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container_end_page 1081
container_issue 4
container_start_page 1071
container_title Injury
container_volume 54
creator Pereira, Betzabeth
Duque, Kharelys
Ramos-Gonzalez, Giselle
Díaz-Solano, Dylana
Wittig, Olga
Zamora, Mariela
Gledhill, Teresa
Cardier, José E.
description •Cutaneous wound healing continues being a serious medical problem.•Cell therapy based on mesenchymal stromal cells (MSC) has emerged as a promising therapeutic alternative to treat skin wounds.•Here we show that MSCs loaded on polyethylene terephthalate (PET) scaffold induce a rapid re-epithelialization of cutaneous wounds. Several clinical studies have shown that cellular therapy based on mesenchymal stromal cells (MSCs) transplantation may accelerate wound healing. One major challenge is the delivery system used for MSCs transplantation. In this work, we evaluated the capacity of a scaffold based on polyethylene terephthalate (PET) to maintain the viability and biological functions of MSCs, in vitro. We examined the capacity of MSCs loaded on PET (MSCs/PET) to induce wound healing in an experimental model of full-thickness wound. Human MSCs were seeded and cultured on PET membranes at 37 °C for 48 h. Adhesion, viability, proliferation, migration, multipotential differentiation and chemokine production were evaluated in cultures of MSCs/PET. The possible therapeutic effect of MSCs/PET on the re-epithelialization of full thickness wounds was examined at day 3 post-wounding in C57BL/6 mice. Histological and immunohistochemical (IH) studies were performed to evaluate wound re-epithelialization and the presence of epithelial progenitor cells (EPC). As controls, wounds without treatment or treated with PET were established. We observed MSCs adhered to PET membranes and maintained their viability, proliferation and migration. They preserved their multipotential capacity of differentiation and ability of chemokine production. MSCs/PET implants promoted an accelerated wound re-epithelialization, after three days post-wounding. It was associated with the presence of EPC Lgr6+ and K6+. Our results show that MSCs/PET implants induce a rapid re-epithelialization of deep- and full-thickness wounds. MSCs/PET implants constitute a potential clinical therapy for treating cutaneous wounds.
doi_str_mv 10.1016/j.injury.2023.02.024
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Several clinical studies have shown that cellular therapy based on mesenchymal stromal cells (MSCs) transplantation may accelerate wound healing. One major challenge is the delivery system used for MSCs transplantation. In this work, we evaluated the capacity of a scaffold based on polyethylene terephthalate (PET) to maintain the viability and biological functions of MSCs, in vitro. We examined the capacity of MSCs loaded on PET (MSCs/PET) to induce wound healing in an experimental model of full-thickness wound. Human MSCs were seeded and cultured on PET membranes at 37 °C for 48 h. Adhesion, viability, proliferation, migration, multipotential differentiation and chemokine production were evaluated in cultures of MSCs/PET. The possible therapeutic effect of MSCs/PET on the re-epithelialization of full thickness wounds was examined at day 3 post-wounding in C57BL/6 mice. Histological and immunohistochemical (IH) studies were performed to evaluate wound re-epithelialization and the presence of epithelial progenitor cells (EPC). As controls, wounds without treatment or treated with PET were established. We observed MSCs adhered to PET membranes and maintained their viability, proliferation and migration. They preserved their multipotential capacity of differentiation and ability of chemokine production. MSCs/PET implants promoted an accelerated wound re-epithelialization, after three days post-wounding. It was associated with the presence of EPC Lgr6+ and K6+. Our results show that MSCs/PET implants induce a rapid re-epithelialization of deep- and full-thickness wounds. 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identifier ISSN: 0020-1383
ispartof Injury, 2023-04, Vol.54 (4), p.1071-1081
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subjects Animals
Chemokines - pharmacology
Humans
Mesenchymal Stem Cell Transplantation - methods
Mesenchymal Stem Cells
Mesenchymal stromal cells
Mice
Mice, Inbred C57BL
MSCs
PET scaffold
Polyethylene Terephthalates - pharmacology
Re-epithelialization
Skin - injuries
Skin injury
Wound Healing
title Wound healing by transplantation of mesenchymal stromal cells loaded on polyethylene terephthalate scaffold: Implications for skin injury treatment
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