Mass Nanotags Mediate Parallel Amplifications on Nanointerfaces for Multiplexed Profiling of RNAs

Mass Nanotags Mediate Parallel Amplifications on Nanointerfaces for Multiplexed Profiling of RNAs

Multiplexed profiling of RNAs aids in a comprehensive understanding of multiparameter-defined cellular processes and pathological states. We herein present a mass nanotags-enabled interfacial assembly system (MNTs-AS) with parallel amplification motors for simultaneous assaying of multiple RNAs in b...

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Veröffentlicht in:Nano letters 2023-03, Vol.23 (5), p.1820-1829
Hauptverfasser: Zhang, Zhenzhen, Xu, Hongmei, Fan, Yinyin, Zhang, Xue, Wang, Wei, Zhu, Jun-Jie, Min, Qianhao
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MicroRNAs - genetics
RNA, Messenger
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container_end_page 1829
container_issue 5
container_start_page 1820
container_title Nano letters
container_volume 23
creator Zhang, Zhenzhen
Xu, Hongmei
Fan, Yinyin
Zhang, Xue
Wang, Wei
Zhu, Jun-Jie
Min, Qianhao
description Multiplexed profiling of RNAs aids in a comprehensive understanding of multiparameter-defined cellular processes and pathological states. We herein present a mass nanotags-enabled interfacial assembly system (MNTs-AS) with parallel amplification motors for simultaneous assaying of multiple RNAs in biosystems by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Four kinds of MNTs encoding corresponding RNA can be cyclically assembled on magnetic beads by target-triggered catalytic hairpin assembly (CHA) machineries on nanointerfaces, generating multiplexed and amplified characteristic ion signals assigned to target RNAs upon MALDI MS interrogation. By virtue of high sensitivity and multiplexing capability, the MNTs-AS-based MS assay allows precision subtyping of diverse breast cancer cells and their exosomes by multiplexed profiling of miRNA-21, miRNA-373, miRNA-155, and manganese superoxide dismutase mRNA via a single MS inquiry. This method provides a promising tool for unraveling multiple RNA-involved biological events in fundamental research and distinguishing different cancer subtypes in clinical practice.
doi_str_mv 10.1021/acs.nanolett.2c04690
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subjects MicroRNAs - genetics
RNA, Messenger
title Mass Nanotags Mediate Parallel Amplifications on Nanointerfaces for Multiplexed Profiling of RNAs
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