Small non-coding RNAome changes during human chondrocyte senescence as potential epigenetic targets in age-related osteoarthritis
Chondrocyte senescence is a decisive component of age-related osteoarthritis, however, the function of small noncoding RNAs (sncRNAs) in chondrocyte senescence remains underexplored. Human hip joint cartilage chondrocytes were cultivated up to passage 4 to induce senescence. RNA samples were extract...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 2023-03, Vol.115 (2), p.110574-110574, Article 110574 |
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| container_title | Genomics (San Diego, Calif.) |
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| creator | Zhang, Qian-Yi Zhou, Hao Zhou, Xiao-Xiao Yu, Feng-Bin Liu, Yu-Yi Chen, Zhi-Yang Ma, Yi-Qun Li, Xi-Lei Tian, Bo |
| description | Chondrocyte senescence is a decisive component of age-related osteoarthritis, however, the function of small noncoding RNAs (sncRNAs) in chondrocyte senescence remains underexplored. Human hip joint cartilage chondrocytes were cultivated up to passage 4 to induce senescence. RNA samples were extracted and then analyzed using small RNA sequencing and qPCR. β-galactosidase staining was used to detect the effect of sncRNA on chondrocyte aging. Results of small RNA sequencing showed that 279 miRNAs, 136 snoRNAs, 30 snRNAs, 102 piRNAs, and 5 rasiRNAs were differentially expressed in senescent chondrocytes. The differential expression of 150 sncRNAs was further validated by qPCR. Transfection of sncRNAs and β-galactosidase staining were also performed to further revealed that hsa-miR-135b-5p, SNORA80B-201, and RNU5E-1-201 have the function to restrain chondrocyte senescence, while has-piR-019102 has the function to promote chondrocyte senescence. Our data suggest that sncRNAs have therapeutic potential as novel epigenetic targets in age-related osteoarthritis. |
| doi_str_mv | 10.1016/j.ygeno.2023.110574 |
| format | Article |
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Human hip joint cartilage chondrocytes were cultivated up to passage 4 to induce senescence. RNA samples were extracted and then analyzed using small RNA sequencing and qPCR. β-galactosidase staining was used to detect the effect of sncRNA on chondrocyte aging. Results of small RNA sequencing showed that 279 miRNAs, 136 snoRNAs, 30 snRNAs, 102 piRNAs, and 5 rasiRNAs were differentially expressed in senescent chondrocytes. The differential expression of 150 sncRNAs was further validated by qPCR. Transfection of sncRNAs and β-galactosidase staining were also performed to further revealed that hsa-miR-135b-5p, SNORA80B-201, and RNU5E-1-201 have the function to restrain chondrocyte senescence, while has-piR-019102 has the function to promote chondrocyte senescence. Our data suggest that sncRNAs have therapeutic potential as novel epigenetic targets in age-related osteoarthritis.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1016/j.ygeno.2023.110574</identifier><identifier>PMID: 36758878</identifier><language>eng</language><publisher>United States</publisher><subject>beta-Galactosidase - genetics ; beta-Galactosidase - metabolism ; Cellular Senescence ; Chondrocytes - metabolism ; Epigenesis, Genetic ; Humans ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Osteoarthritis - genetics ; RNA, Small Untranslated - metabolism</subject><ispartof>Genomics (San Diego, Calif.), 2023-03, Vol.115 (2), p.110574-110574, Article 110574</ispartof><rights>Copyright © 2023. Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2654-63adc2e1864be162b440089ecdeefb9d5257df7e2f539f4fdcbfa94a6c24e1eb3</citedby><cites>FETCH-LOGICAL-c2654-63adc2e1864be162b440089ecdeefb9d5257df7e2f539f4fdcbfa94a6c24e1eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36758878$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Qian-Yi</creatorcontrib><creatorcontrib>Zhou, Hao</creatorcontrib><creatorcontrib>Zhou, Xiao-Xiao</creatorcontrib><creatorcontrib>Yu, Feng-Bin</creatorcontrib><creatorcontrib>Liu, Yu-Yi</creatorcontrib><creatorcontrib>Chen, Zhi-Yang</creatorcontrib><creatorcontrib>Ma, Yi-Qun</creatorcontrib><creatorcontrib>Li, Xi-Lei</creatorcontrib><creatorcontrib>Tian, Bo</creatorcontrib><title>Small non-coding RNAome changes during human chondrocyte senescence as potential epigenetic targets in age-related osteoarthritis</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>Chondrocyte senescence is a decisive component of age-related osteoarthritis, however, the function of small noncoding RNAs (sncRNAs) in chondrocyte senescence remains underexplored. Human hip joint cartilage chondrocytes were cultivated up to passage 4 to induce senescence. RNA samples were extracted and then analyzed using small RNA sequencing and qPCR. β-galactosidase staining was used to detect the effect of sncRNA on chondrocyte aging. Results of small RNA sequencing showed that 279 miRNAs, 136 snoRNAs, 30 snRNAs, 102 piRNAs, and 5 rasiRNAs were differentially expressed in senescent chondrocytes. The differential expression of 150 sncRNAs was further validated by qPCR. Transfection of sncRNAs and β-galactosidase staining were also performed to further revealed that hsa-miR-135b-5p, SNORA80B-201, and RNU5E-1-201 have the function to restrain chondrocyte senescence, while has-piR-019102 has the function to promote chondrocyte senescence. Our data suggest that sncRNAs have therapeutic potential as novel epigenetic targets in age-related osteoarthritis.</description><subject>beta-Galactosidase - genetics</subject><subject>beta-Galactosidase - metabolism</subject><subject>Cellular Senescence</subject><subject>Chondrocytes - metabolism</subject><subject>Epigenesis, Genetic</subject><subject>Humans</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Osteoarthritis - genetics</subject><subject>RNA, Small Untranslated - metabolism</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtv2zAQhIkiRe2k_QUFAh5zkcuXKOpoBEkbIEiBPs4ERa5kGhLpkNTBx_7zyHWS0wKDmd3ZD6GvlGwoofLbfnMcIMQNI4xvKCV1Iz6gNSWqrZQU8gKtiVKqamrBV-gy5z0hpOWKfUIrLptaqUat0b_fkxlHHGKobHQ-DPjX0zZOgO3OhAEydnM6qbt5MmERY3Ap2mMBnCFAthAsYJPxIRYIxZsRw8EvtaB4i4tJA5SMfcBmgCrBaAo4HHOBaFLZJV98_ow-9mbM8OV1XqG_93d_bn9Ujz-_P9xuHyvLZC0qyY2zDOjyWgdUsk4IsrwK1gH0XetqVjeub4D1NW970Tvb9aYVRlomgELHr9DNee8hxecZctGTX_qPowkQ56xZ09SSspa1i5WfrTbFnBP0-pD8ZNJRU6JP7PVe_2evT-z1mf2Sun49MHcTuPfMG2z-AjMahYs</recordid><startdate>202303</startdate><enddate>202303</enddate><creator>Zhang, Qian-Yi</creator><creator>Zhou, Hao</creator><creator>Zhou, Xiao-Xiao</creator><creator>Yu, Feng-Bin</creator><creator>Liu, Yu-Yi</creator><creator>Chen, Zhi-Yang</creator><creator>Ma, Yi-Qun</creator><creator>Li, Xi-Lei</creator><creator>Tian, Bo</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202303</creationdate><title>Small non-coding RNAome changes during human chondrocyte senescence as potential epigenetic targets in age-related osteoarthritis</title><author>Zhang, Qian-Yi ; Zhou, Hao ; Zhou, Xiao-Xiao ; Yu, Feng-Bin ; Liu, Yu-Yi ; Chen, Zhi-Yang ; Ma, Yi-Qun ; Li, Xi-Lei ; Tian, Bo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2654-63adc2e1864be162b440089ecdeefb9d5257df7e2f539f4fdcbfa94a6c24e1eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>beta-Galactosidase - genetics</topic><topic>beta-Galactosidase - metabolism</topic><topic>Cellular Senescence</topic><topic>Chondrocytes - metabolism</topic><topic>Epigenesis, Genetic</topic><topic>Humans</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Osteoarthritis - genetics</topic><topic>RNA, Small Untranslated - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Qian-Yi</creatorcontrib><creatorcontrib>Zhou, Hao</creatorcontrib><creatorcontrib>Zhou, Xiao-Xiao</creatorcontrib><creatorcontrib>Yu, Feng-Bin</creatorcontrib><creatorcontrib>Liu, Yu-Yi</creatorcontrib><creatorcontrib>Chen, Zhi-Yang</creatorcontrib><creatorcontrib>Ma, Yi-Qun</creatorcontrib><creatorcontrib>Li, Xi-Lei</creatorcontrib><creatorcontrib>Tian, Bo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Qian-Yi</au><au>Zhou, Hao</au><au>Zhou, Xiao-Xiao</au><au>Yu, Feng-Bin</au><au>Liu, Yu-Yi</au><au>Chen, Zhi-Yang</au><au>Ma, Yi-Qun</au><au>Li, Xi-Lei</au><au>Tian, Bo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Small non-coding RNAome changes during human chondrocyte senescence as potential epigenetic targets in age-related osteoarthritis</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>2023-03</date><risdate>2023</risdate><volume>115</volume><issue>2</issue><spage>110574</spage><epage>110574</epage><pages>110574-110574</pages><artnum>110574</artnum><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>Chondrocyte senescence is a decisive component of age-related osteoarthritis, however, the function of small noncoding RNAs (sncRNAs) in chondrocyte senescence remains underexplored. Human hip joint cartilage chondrocytes were cultivated up to passage 4 to induce senescence. RNA samples were extracted and then analyzed using small RNA sequencing and qPCR. β-galactosidase staining was used to detect the effect of sncRNA on chondrocyte aging. Results of small RNA sequencing showed that 279 miRNAs, 136 snoRNAs, 30 snRNAs, 102 piRNAs, and 5 rasiRNAs were differentially expressed in senescent chondrocytes. The differential expression of 150 sncRNAs was further validated by qPCR. Transfection of sncRNAs and β-galactosidase staining were also performed to further revealed that hsa-miR-135b-5p, SNORA80B-201, and RNU5E-1-201 have the function to restrain chondrocyte senescence, while has-piR-019102 has the function to promote chondrocyte senescence. Our data suggest that sncRNAs have therapeutic potential as novel epigenetic targets in age-related osteoarthritis.</abstract><cop>United States</cop><pmid>36758878</pmid><doi>10.1016/j.ygeno.2023.110574</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | beta-Galactosidase - genetics beta-Galactosidase - metabolism Cellular Senescence Chondrocytes - metabolism Epigenesis, Genetic Humans MicroRNAs - genetics MicroRNAs - metabolism Osteoarthritis - genetics RNA, Small Untranslated - metabolism |
| title | Small non-coding RNAome changes during human chondrocyte senescence as potential epigenetic targets in age-related osteoarthritis |
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