Analysis of the in vitro function and internalization ability of a humanized EGFR antibody AE01 expressed by Chinese hamster ovary cells

The primary objective of this study was to obtain humanized EGFR antibody and to study it in vitro binding and endocytosis to A431 epidermoid carcinoma cells overexpressing EGFR. Firstly, humanized anti-EGFR AE01 was stably expressed in CHO system. The expression of AE01 was detected by SDS-PAGE and...

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Veröffentlicht in:	Protein expression and purification 2023-06, Vol.206, p.106243-106243, Article 106243
Hauptverfasser:	Wu, Hao, Ding, Xuefeng, Chen, Yun, Cai, Yanfei, Yang, Zhaoqi, Xu, Qinghe, Jin, Jian
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies Antibody Cell Line, Tumor Chinese hamster ovary cells CHO Cells Cricetinae Cricetulus Epidermal growth factor receptor ErbB Receptors Expression Humanized Internalization Mice
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