Corynebacterium megadyptis sp. nov. with two subspecies, Corynebacterium megadyptis subsp. megadyptis subsp. nov. and Corynebacterium megadyptis subsp. dunedinense subsp. nov. isolated from yellow-eyed penguins
Novel strains, 3B and 7B , were isolated from the oral cavities of young chicks of yellow-eyed penguins (hoiho), . A polyphasic taxonomic characterization of these strains revealed chemotaxonomic, biochemical and morphological features that are consistent with those of the genus . The 16S rRNA gene...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2023-02, Vol.73 (2) |
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| creator | Nouioui, Imen Saunderson, Sarah C Midwinter, Anne C Young, Melanie J McInnes, Kate M Watts, Jim Sangal, Vartul |
| description | Novel
strains, 3B
and 7B
, were isolated from the oral cavities of young chicks of yellow-eyed penguins (hoiho),
. A polyphasic taxonomic characterization of these strains revealed chemotaxonomic, biochemical and morphological features that are consistent with those of the genus
. The 16S rRNA gene sequence similarity values between the strains and their closest phylogenetic neighbour,
CCUG 47525
were 99.07 %, values that are in line with their phylogenomic positions within the evolutionary radiation of the genus
. Digital DNA-DNA hybridization values and average nucleotide identities between the genome sequences of the two strains and related
species were well below the defined threshold values (70 and 95-96 %, respectively) for prokaryotic species delineation. The genome size of these strains varied between 2.45-2.46 Mb with G+C content 62.7-62.9 mol%. Strains 3B
and 7B
were Gram-stain positive bacilli that were able to grow in presence of 0-10 % (w/v) NaCl and at temperature ranging between 20-37 °C. The major fatty acids (>15 %) were C
and C
9
, and the mycolic acid profile included 32-36 carbon atoms. We propose that these strains represent a novel species,
sp. nov. with 3B
(=DSM 111184
=NZRM 4755
) as the type strain. Phylogenomically, strains 3B
and 7B
belong to two lineages with subtle differences in MALDI-TOF spectra, chemotaxonomic profiles and phenotypic properties. The fatty acid profile of strain 3B
contains C
as a predominant type (>15 %), which is a minor component in strain 7B
. Strain 7B
can oxidize
-acetyl-d-glucosamine, l-serine, α-hydroxy-butyric acid, l-malic acid, l-glutamic acid, bromo-succinic acid and l-lactic acid, characteristics not observed in strain 3B
. Therefore, we propose that these strains represent two subspecies, namely
subsp.
subsp. nov. (type strain, 3B
=DSM 111184
=NZRM 4755
) and
subsp.
subsp. nov. (type strain, 7B
=DSM 111183
=NZRM 4756
). |
| doi_str_mv | 10.1099/ijsem.0.005713 |
| format | Article |
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strains, 3B
and 7B
, were isolated from the oral cavities of young chicks of yellow-eyed penguins (hoiho),
. A polyphasic taxonomic characterization of these strains revealed chemotaxonomic, biochemical and morphological features that are consistent with those of the genus
. The 16S rRNA gene sequence similarity values between the strains and their closest phylogenetic neighbour,
CCUG 47525
were 99.07 %, values that are in line with their phylogenomic positions within the evolutionary radiation of the genus
. Digital DNA-DNA hybridization values and average nucleotide identities between the genome sequences of the two strains and related
species were well below the defined threshold values (70 and 95-96 %, respectively) for prokaryotic species delineation. The genome size of these strains varied between 2.45-2.46 Mb with G+C content 62.7-62.9 mol%. Strains 3B
and 7B
were Gram-stain positive bacilli that were able to grow in presence of 0-10 % (w/v) NaCl and at temperature ranging between 20-37 °C. The major fatty acids (>15 %) were C
and C
9
, and the mycolic acid profile included 32-36 carbon atoms. We propose that these strains represent a novel species,
sp. nov. with 3B
(=DSM 111184
=NZRM 4755
) as the type strain. Phylogenomically, strains 3B
and 7B
belong to two lineages with subtle differences in MALDI-TOF spectra, chemotaxonomic profiles and phenotypic properties. The fatty acid profile of strain 3B
contains C
as a predominant type (>15 %), which is a minor component in strain 7B
. Strain 7B
can oxidize
-acetyl-d-glucosamine, l-serine, α-hydroxy-butyric acid, l-malic acid, l-glutamic acid, bromo-succinic acid and l-lactic acid, characteristics not observed in strain 3B
. Therefore, we propose that these strains represent two subspecies, namely
subsp.
subsp. nov. (type strain, 3B
=DSM 111184
=NZRM 4755
) and
subsp.
subsp. nov. (type strain, 7B
=DSM 111183
=NZRM 4756
).</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijsem.0.005713</identifier><identifier>PMID: 36749700</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Bacterial Typing Techniques ; Base Composition ; Corynebacterium ; DNA, Bacterial - genetics ; Fatty Acids - chemistry ; Nucleic Acid Hybridization ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Spheniscidae</subject><ispartof>International journal of systematic and evolutionary microbiology, 2023-02, Vol.73 (2)</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c335t-db7afc38ca5288452ed0366ffd64735239b93a5a88af7efabe4b94b9c2de99cf3</citedby><cites>FETCH-LOGICAL-c335t-db7afc38ca5288452ed0366ffd64735239b93a5a88af7efabe4b94b9c2de99cf3</cites><orcidid>0000-0002-7405-8446</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,3748,27931,27932</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36749700$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nouioui, Imen</creatorcontrib><creatorcontrib>Saunderson, Sarah C</creatorcontrib><creatorcontrib>Midwinter, Anne C</creatorcontrib><creatorcontrib>Young, Melanie J</creatorcontrib><creatorcontrib>McInnes, Kate M</creatorcontrib><creatorcontrib>Watts, Jim</creatorcontrib><creatorcontrib>Sangal, Vartul</creatorcontrib><title>Corynebacterium megadyptis sp. nov. with two subspecies, Corynebacterium megadyptis subsp. megadyptis subsp. nov. and Corynebacterium megadyptis subsp. dunedinense subsp. nov. isolated from yellow-eyed penguins</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>Novel
strains, 3B
and 7B
, were isolated from the oral cavities of young chicks of yellow-eyed penguins (hoiho),
. A polyphasic taxonomic characterization of these strains revealed chemotaxonomic, biochemical and morphological features that are consistent with those of the genus
. The 16S rRNA gene sequence similarity values between the strains and their closest phylogenetic neighbour,
CCUG 47525
were 99.07 %, values that are in line with their phylogenomic positions within the evolutionary radiation of the genus
. Digital DNA-DNA hybridization values and average nucleotide identities between the genome sequences of the two strains and related
species were well below the defined threshold values (70 and 95-96 %, respectively) for prokaryotic species delineation. The genome size of these strains varied between 2.45-2.46 Mb with G+C content 62.7-62.9 mol%. Strains 3B
and 7B
were Gram-stain positive bacilli that were able to grow in presence of 0-10 % (w/v) NaCl and at temperature ranging between 20-37 °C. The major fatty acids (>15 %) were C
and C
9
, and the mycolic acid profile included 32-36 carbon atoms. We propose that these strains represent a novel species,
sp. nov. with 3B
(=DSM 111184
=NZRM 4755
) as the type strain. Phylogenomically, strains 3B
and 7B
belong to two lineages with subtle differences in MALDI-TOF spectra, chemotaxonomic profiles and phenotypic properties. The fatty acid profile of strain 3B
contains C
as a predominant type (>15 %), which is a minor component in strain 7B
. Strain 7B
can oxidize
-acetyl-d-glucosamine, l-serine, α-hydroxy-butyric acid, l-malic acid, l-glutamic acid, bromo-succinic acid and l-lactic acid, characteristics not observed in strain 3B
. Therefore, we propose that these strains represent two subspecies, namely
subsp.
subsp. nov. (type strain, 3B
=DSM 111184
=NZRM 4755
) and
subsp.
subsp. nov. (type strain, 7B
=DSM 111183
=NZRM 4756
).</description><subject>Animals</subject><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>Corynebacterium</subject><subject>DNA, Bacterial - genetics</subject><subject>Fatty Acids - chemistry</subject><subject>Nucleic Acid Hybridization</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Spheniscidae</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtLxDAUhYMozji6dSlZurA1bfpcyuALBtzouqTJzZihTWpv69C_6S-y81AQRIQLuRzOd7jkEHIeMD9geX5tVgi1z3zG4jTgB2QaREnixYxHh997mEzICeKKsVFg7JhMeJJGecrYlHzMXTtYKIXsoDV9TWtYCjU0nUGKjU-te_fp2nSvtFs7in2JDUgDeEX_Ajc2_xdlmyas-gesegvKWLAIP2iDrhIdKKpbV9MBqsqtPRhGoQG77I3FU3KkRYVwtn9n5OXu9nn-4C2e7h_nNwtPch53nipToSXPpIjDLIviEBTjSaK1SqKUxyHPy5yLWGSZ0CloUUJU5uPIUEGeS81n5HKX27TurQfsitqgHA8SFlyPRZimUZhk46-PVn9nla1DbEEXTWtq0Q5FwIpNj8W2x4IVux5H4GKf3Zc1qG_7V3H8EyicoXI</recordid><startdate>202302</startdate><enddate>202302</enddate><creator>Nouioui, Imen</creator><creator>Saunderson, Sarah C</creator><creator>Midwinter, Anne C</creator><creator>Young, Melanie J</creator><creator>McInnes, Kate M</creator><creator>Watts, Jim</creator><creator>Sangal, Vartul</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7405-8446</orcidid></search><sort><creationdate>202302</creationdate><title>Corynebacterium megadyptis sp. nov. with two subspecies, Corynebacterium megadyptis subsp. megadyptis subsp. nov. and Corynebacterium megadyptis subsp. dunedinense subsp. nov. isolated from yellow-eyed penguins</title><author>Nouioui, Imen ; Saunderson, Sarah C ; Midwinter, Anne C ; Young, Melanie J ; McInnes, Kate M ; Watts, Jim ; Sangal, Vartul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c335t-db7afc38ca5288452ed0366ffd64735239b93a5a88af7efabe4b94b9c2de99cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Bacterial Typing Techniques</topic><topic>Base Composition</topic><topic>Corynebacterium</topic><topic>DNA, Bacterial - genetics</topic><topic>Fatty Acids - chemistry</topic><topic>Nucleic Acid Hybridization</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Spheniscidae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nouioui, Imen</creatorcontrib><creatorcontrib>Saunderson, Sarah C</creatorcontrib><creatorcontrib>Midwinter, Anne C</creatorcontrib><creatorcontrib>Young, Melanie J</creatorcontrib><creatorcontrib>McInnes, Kate M</creatorcontrib><creatorcontrib>Watts, Jim</creatorcontrib><creatorcontrib>Sangal, Vartul</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nouioui, Imen</au><au>Saunderson, Sarah C</au><au>Midwinter, Anne C</au><au>Young, Melanie J</au><au>McInnes, Kate M</au><au>Watts, Jim</au><au>Sangal, Vartul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Corynebacterium megadyptis sp. nov. with two subspecies, Corynebacterium megadyptis subsp. megadyptis subsp. nov. and Corynebacterium megadyptis subsp. dunedinense subsp. nov. isolated from yellow-eyed penguins</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2023-02</date><risdate>2023</risdate><volume>73</volume><issue>2</issue><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>Novel
strains, 3B
and 7B
, were isolated from the oral cavities of young chicks of yellow-eyed penguins (hoiho),
. A polyphasic taxonomic characterization of these strains revealed chemotaxonomic, biochemical and morphological features that are consistent with those of the genus
. The 16S rRNA gene sequence similarity values between the strains and their closest phylogenetic neighbour,
CCUG 47525
were 99.07 %, values that are in line with their phylogenomic positions within the evolutionary radiation of the genus
. Digital DNA-DNA hybridization values and average nucleotide identities between the genome sequences of the two strains and related
species were well below the defined threshold values (70 and 95-96 %, respectively) for prokaryotic species delineation. The genome size of these strains varied between 2.45-2.46 Mb with G+C content 62.7-62.9 mol%. Strains 3B
and 7B
were Gram-stain positive bacilli that were able to grow in presence of 0-10 % (w/v) NaCl and at temperature ranging between 20-37 °C. The major fatty acids (>15 %) were C
and C
9
, and the mycolic acid profile included 32-36 carbon atoms. We propose that these strains represent a novel species,
sp. nov. with 3B
(=DSM 111184
=NZRM 4755
) as the type strain. Phylogenomically, strains 3B
and 7B
belong to two lineages with subtle differences in MALDI-TOF spectra, chemotaxonomic profiles and phenotypic properties. The fatty acid profile of strain 3B
contains C
as a predominant type (>15 %), which is a minor component in strain 7B
. Strain 7B
can oxidize
-acetyl-d-glucosamine, l-serine, α-hydroxy-butyric acid, l-malic acid, l-glutamic acid, bromo-succinic acid and l-lactic acid, characteristics not observed in strain 3B
. Therefore, we propose that these strains represent two subspecies, namely
subsp.
subsp. nov. (type strain, 3B
=DSM 111184
=NZRM 4755
) and
subsp.
subsp. nov. (type strain, 7B
=DSM 111183
=NZRM 4756
).</abstract><cop>England</cop><pmid>36749700</pmid><doi>10.1099/ijsem.0.005713</doi><orcidid>https://orcid.org/0000-0002-7405-8446</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1466-5026 |
| ispartof | International journal of systematic and evolutionary microbiology, 2023-02, Vol.73 (2) |
| issn | 1466-5026 1466-5034 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2774268001 |
| source | MEDLINE; Microbiology Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Bacterial Typing Techniques Base Composition Corynebacterium DNA, Bacterial - genetics Fatty Acids - chemistry Nucleic Acid Hybridization Phylogeny RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Spheniscidae |
| title | Corynebacterium megadyptis sp. nov. with two subspecies, Corynebacterium megadyptis subsp. megadyptis subsp. nov. and Corynebacterium megadyptis subsp. dunedinense subsp. nov. isolated from yellow-eyed penguins |
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