Transcriptome investigation on the multicellular behavior of Bacillus velezensis Bs916 anchoring surfactin
To provide valuable information for a comprehensive understanding of the multicellular behavior of Bacillus velezensis Bs916 regulated by surfactin and other natural signals by Transcriptome. Transcriptomics revealed a distinct effect on gene expression alterations caused by disruption of the surfac...
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| Veröffentlicht in: | Journal of applied microbiology 2023-01, Vol.134 (1) |
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| container_title | Journal of applied microbiology |
| container_volume | 134 |
| creator | Dong, Fei Ren, Shiying Liu, Jiachen Bilal, Muhammad Li, Bin Chen, Xin Liu, Xuehui Zhong, Haijing Zang, Shanshan Yuan, Mengxian Luo, Chuping Jiang, Jihong |
| description | To provide valuable information for a comprehensive understanding of the multicellular behavior of Bacillus velezensis Bs916 regulated by surfactin and other natural signals by Transcriptome.
Transcriptomics revealed a distinct effect on gene expression alterations caused by disruption of the surfactin gene cluster(Δsrf) and 100 µg/ml surfactin addition(Δsrf + SRF). A total of 1573 differential expression genes were identified among Bs916, Δsrf, and Δsrf + SRF and grouped into eight categories based on their expression profiles. RT-qPCR analysis of 30 candidate genes showed high consistency with those of transcriptome. Additionally, the expression of eight candidate genes regulated by surfactin in a dose-dependent manner was revealed by lacZ fusion. Based on the above evidence, we proposed that surfactin can act as an extracellular signal for monitoring biofilm formation in Bs916 by directly regulating the expression of AbrB, DegS-degU, and SinI-SinR, and indirectly regulating the phosphorylation of ComA and Spo0A.
The biofilm of Δsrf was unable to restore significantly by surfactin addition, combined inclusion of surfactin (SRF), exopolysaccharide (EPS), and γ-poly-dl-glutamic acid (γ-PGA), results in significant restoration of Δsrf biofilm formation, thereby a preliminary model was presented about the molecular mechanism by which the signaling molecule surfactin regulates Bs916 multicellular behavior. |
| doi_str_mv | 10.1093/jambio/lxac016 |
| format | Article |
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Transcriptomics revealed a distinct effect on gene expression alterations caused by disruption of the surfactin gene cluster(Δsrf) and 100 µg/ml surfactin addition(Δsrf + SRF). A total of 1573 differential expression genes were identified among Bs916, Δsrf, and Δsrf + SRF and grouped into eight categories based on their expression profiles. RT-qPCR analysis of 30 candidate genes showed high consistency with those of transcriptome. Additionally, the expression of eight candidate genes regulated by surfactin in a dose-dependent manner was revealed by lacZ fusion. Based on the above evidence, we proposed that surfactin can act as an extracellular signal for monitoring biofilm formation in Bs916 by directly regulating the expression of AbrB, DegS-degU, and SinI-SinR, and indirectly regulating the phosphorylation of ComA and Spo0A.
The biofilm of Δsrf was unable to restore significantly by surfactin addition, combined inclusion of surfactin (SRF), exopolysaccharide (EPS), and γ-poly-dl-glutamic acid (γ-PGA), results in significant restoration of Δsrf biofilm formation, thereby a preliminary model was presented about the molecular mechanism by which the signaling molecule surfactin regulates Bs916 multicellular behavior.</description><identifier>ISSN: 1365-2672</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1093/jambio/lxac016</identifier><identifier>PMID: 36626730</identifier><language>eng</language><publisher>England</publisher><subject>Bacillus - physiology ; Bacillus subtilis - metabolism ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biofilms ; Gene Expression Profiling ; Gene Expression Regulation, Bacterial ; Lipopeptides - metabolism ; Lipopeptides - pharmacology ; Multigene Family ; Transcriptome</subject><ispartof>Journal of applied microbiology, 2023-01, Vol.134 (1)</ispartof><rights>The Author(s) 2022. Published by Oxford University Press on behalf of Applied Microbiology International.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c295t-7ae45bb82c466815558a1d61d1b2d31fbc06fd528b8022b0d3092d4c566892653</citedby><cites>FETCH-LOGICAL-c295t-7ae45bb82c466815558a1d61d1b2d31fbc06fd528b8022b0d3092d4c566892653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36626730$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dong, Fei</creatorcontrib><creatorcontrib>Ren, Shiying</creatorcontrib><creatorcontrib>Liu, Jiachen</creatorcontrib><creatorcontrib>Bilal, Muhammad</creatorcontrib><creatorcontrib>Li, Bin</creatorcontrib><creatorcontrib>Chen, Xin</creatorcontrib><creatorcontrib>Liu, Xuehui</creatorcontrib><creatorcontrib>Zhong, Haijing</creatorcontrib><creatorcontrib>Zang, Shanshan</creatorcontrib><creatorcontrib>Yuan, Mengxian</creatorcontrib><creatorcontrib>Luo, Chuping</creatorcontrib><creatorcontrib>Jiang, Jihong</creatorcontrib><title>Transcriptome investigation on the multicellular behavior of Bacillus velezensis Bs916 anchoring surfactin</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>To provide valuable information for a comprehensive understanding of the multicellular behavior of Bacillus velezensis Bs916 regulated by surfactin and other natural signals by Transcriptome.
Transcriptomics revealed a distinct effect on gene expression alterations caused by disruption of the surfactin gene cluster(Δsrf) and 100 µg/ml surfactin addition(Δsrf + SRF). A total of 1573 differential expression genes were identified among Bs916, Δsrf, and Δsrf + SRF and grouped into eight categories based on their expression profiles. RT-qPCR analysis of 30 candidate genes showed high consistency with those of transcriptome. Additionally, the expression of eight candidate genes regulated by surfactin in a dose-dependent manner was revealed by lacZ fusion. Based on the above evidence, we proposed that surfactin can act as an extracellular signal for monitoring biofilm formation in Bs916 by directly regulating the expression of AbrB, DegS-degU, and SinI-SinR, and indirectly regulating the phosphorylation of ComA and Spo0A.
The biofilm of Δsrf was unable to restore significantly by surfactin addition, combined inclusion of surfactin (SRF), exopolysaccharide (EPS), and γ-poly-dl-glutamic acid (γ-PGA), results in significant restoration of Δsrf biofilm formation, thereby a preliminary model was presented about the molecular mechanism by which the signaling molecule surfactin regulates Bs916 multicellular behavior.</description><subject>Bacillus - physiology</subject><subject>Bacillus subtilis - metabolism</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biofilms</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Lipopeptides - metabolism</subject><subject>Lipopeptides - pharmacology</subject><subject>Multigene Family</subject><subject>Transcriptome</subject><issn>1365-2672</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkM1LAzEQxYMotlavHiVHL9vmY5PdPdriFxS81HNIstk2ZTepSbaof71bWkUYmOHxm8fMA-AWoylGFZ1tZaesn7WfUiPMz8AYU84ywgty_m8egasYtwhhihi_BCPK-SBTNAbbVZAu6mB3yXcGWrc3Mdm1TNY7OFTaGNj1bbLatG3fygCV2ci99QH6Bs6ltoMc4d605tu4aCOcxwpzKJ3e-GDdGsY-NFIn667BRSPbaG5OfQLenx5Xi5ds-fb8unhYZppULGWFNDlTqiQ657zEjLFS4prjGitSU9wojXhTM1KqEhGiUE1RRepcs4GuCGd0Au6PvrvgP_rhHdHZeDhfOuP7KEhR0AKXNKcDOj2iOvgYg2nELthOhi-BkTjkK475ilO-w8LdybtXnan_8N9A6Q9lbnoh</recordid><startdate>20230123</startdate><enddate>20230123</enddate><creator>Dong, Fei</creator><creator>Ren, Shiying</creator><creator>Liu, Jiachen</creator><creator>Bilal, Muhammad</creator><creator>Li, Bin</creator><creator>Chen, Xin</creator><creator>Liu, Xuehui</creator><creator>Zhong, Haijing</creator><creator>Zang, Shanshan</creator><creator>Yuan, Mengxian</creator><creator>Luo, Chuping</creator><creator>Jiang, Jihong</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20230123</creationdate><title>Transcriptome investigation on the multicellular behavior of Bacillus velezensis Bs916 anchoring surfactin</title><author>Dong, Fei ; Ren, Shiying ; Liu, Jiachen ; Bilal, Muhammad ; Li, Bin ; Chen, Xin ; Liu, Xuehui ; Zhong, Haijing ; Zang, Shanshan ; Yuan, Mengxian ; Luo, Chuping ; Jiang, Jihong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c295t-7ae45bb82c466815558a1d61d1b2d31fbc06fd528b8022b0d3092d4c566892653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Bacillus - physiology</topic><topic>Bacillus subtilis - metabolism</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biofilms</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Lipopeptides - metabolism</topic><topic>Lipopeptides - pharmacology</topic><topic>Multigene Family</topic><topic>Transcriptome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dong, Fei</creatorcontrib><creatorcontrib>Ren, Shiying</creatorcontrib><creatorcontrib>Liu, Jiachen</creatorcontrib><creatorcontrib>Bilal, Muhammad</creatorcontrib><creatorcontrib>Li, Bin</creatorcontrib><creatorcontrib>Chen, Xin</creatorcontrib><creatorcontrib>Liu, Xuehui</creatorcontrib><creatorcontrib>Zhong, Haijing</creatorcontrib><creatorcontrib>Zang, Shanshan</creatorcontrib><creatorcontrib>Yuan, Mengxian</creatorcontrib><creatorcontrib>Luo, Chuping</creatorcontrib><creatorcontrib>Jiang, Jihong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dong, Fei</au><au>Ren, Shiying</au><au>Liu, Jiachen</au><au>Bilal, Muhammad</au><au>Li, Bin</au><au>Chen, Xin</au><au>Liu, Xuehui</au><au>Zhong, Haijing</au><au>Zang, Shanshan</au><au>Yuan, Mengxian</au><au>Luo, Chuping</au><au>Jiang, Jihong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptome investigation on the multicellular behavior of Bacillus velezensis Bs916 anchoring surfactin</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2023-01-23</date><risdate>2023</risdate><volume>134</volume><issue>1</issue><issn>1365-2672</issn><eissn>1365-2672</eissn><abstract>To provide valuable information for a comprehensive understanding of the multicellular behavior of Bacillus velezensis Bs916 regulated by surfactin and other natural signals by Transcriptome.
Transcriptomics revealed a distinct effect on gene expression alterations caused by disruption of the surfactin gene cluster(Δsrf) and 100 µg/ml surfactin addition(Δsrf + SRF). A total of 1573 differential expression genes were identified among Bs916, Δsrf, and Δsrf + SRF and grouped into eight categories based on their expression profiles. RT-qPCR analysis of 30 candidate genes showed high consistency with those of transcriptome. Additionally, the expression of eight candidate genes regulated by surfactin in a dose-dependent manner was revealed by lacZ fusion. Based on the above evidence, we proposed that surfactin can act as an extracellular signal for monitoring biofilm formation in Bs916 by directly regulating the expression of AbrB, DegS-degU, and SinI-SinR, and indirectly regulating the phosphorylation of ComA and Spo0A.
The biofilm of Δsrf was unable to restore significantly by surfactin addition, combined inclusion of surfactin (SRF), exopolysaccharide (EPS), and γ-poly-dl-glutamic acid (γ-PGA), results in significant restoration of Δsrf biofilm formation, thereby a preliminary model was presented about the molecular mechanism by which the signaling molecule surfactin regulates Bs916 multicellular behavior.</abstract><cop>England</cop><pmid>36626730</pmid><doi>10.1093/jambio/lxac016</doi></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Bacillus - physiology Bacillus subtilis - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Biofilms Gene Expression Profiling Gene Expression Regulation, Bacterial Lipopeptides - metabolism Lipopeptides - pharmacology Multigene Family Transcriptome |
| title | Transcriptome investigation on the multicellular behavior of Bacillus velezensis Bs916 anchoring surfactin |
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