Efficient resolution of 4‐chlormandelic acid enantiomers using lipase@UiO‐67(Zr) zirconium–organic frameworks in organic solvent

A new biocatalyst PCL@UiO‐67(Zr) was successfully synthesized by immobilized lipases on metal–organic frameworks (MOFs) materials. Compare with free lipases, zirconium foundation organic framework material UiO‐67(Zr) modification on immobilized lipases Pseudomonas cepacia lipase (PCL) great boosts their enantioselectivity in the kinetic resolution racemic 4‐chloro‐mandelic acid (4‐ClMA) on the organic solvent. The acquired bio‐composite PCL@UiO‐67(Zr) was fully characterized by powder X‐ray diffraction (PXRD), Fourier transform infrared (FT‐IR) spectroscopy, N2 adsorption–desorption isotherm and aperture distribution map, and scanning electron microscopy (SEM). The catalytic performance of PCL@UiO‐67(Zr), such as temperature, reaction time, and lipase quantity, were deeply explored. The experiment results showed resolution racemic 4‐ClMA optimum conditions that 20 mmol/L of (R, S)‐4‐chloromandelic acid, 120 mmol/L vinyl acetate, 30‐mg immobilized lipases PCL@UiO‐67(Zr), 2 mL of MTBE, 500 rpm, and under the 55°C reaction 18 h. In this optimum conditions, c and eep could reach up to 47.6% and 98.7%, respectively. A new biocatalyst PCL@UiO‐67(Zr) was successfully synthesized and efficient enantioselectivity in the kinetic resolution racemic 4‐chloro‐mandelic acid (4‐ClMA) on the organic solvent.