Engineering the Substrate Specificity of Toluene Degrading Enzyme XylM Using Biosensor XylS and Machine Learning

Enzyme engineering using machine learning has been developed in recent years. However, to obtain a large amount of data on enzyme activities for training data, it is necessary to develop a high-throughput and accurate method for evaluating enzyme activities. Here, we examined whether a biosensor-bas...

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Veröffentlicht in:	ACS synthetic biology 2023-02, Vol.12 (2), p.572-582
Hauptverfasser:	Ogawa, Yuki, Saito, Yutaka, Yamaguchi, Hideki, Katsuyama, Yohei, Ohnishi, Yasuo
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Bacterial Proteins - metabolism Biosensing Techniques Machine Learning Plasmids Pseudomonas putida - genetics Pseudomonas putida - metabolism Substrate Specificity Toluene - metabolism
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