Decreased frequency of regulatory T cells and level of helios gene expression in secondary progressive multiple sclerosis patients: Evidence about the development of multiple sclerosis
[Display omitted] •Helios gene expression decreased in SPMS patients.•The percentage of Helios+ Tregs was low in the peripheral blood of SPMS patients.•One of the immunological mechanisms involved in SPMS may be a deficiency in Tregs.•The percentage of CD4+CD25+ T lymphocytes did not show a signific...
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| Veröffentlicht in: | International immunopharmacology 2023-03, Vol.116, p.109797-109797, Article 109797 |
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| creator | Tarighi, Mona Shahbazi, Mehdi Saadat, Payam Daraei, Abdolreza Alizadeh khatir, Ali Rahimifard, Kimiya Mohammadnia-Afrouzi, Mousa |
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•Helios gene expression decreased in SPMS patients.•The percentage of Helios+ Tregs was low in the peripheral blood of SPMS patients.•One of the immunological mechanisms involved in SPMS may be a deficiency in Tregs.•The percentage of CD4+CD25+ T lymphocytes did not show a significant difference.
Multiple sclerosis (MS) is an aggressive disease characterized by central nervous system (CNS) inflammatory and demyelinating lesions. Tolerance failure is implicated in the development of several autoimmune disorders, including MS. Due to their involvement in maintaining environmental tolerance, regulatory T cells (Tregs) are regarded as efficient immune cells. We examined the frequency of Tregs in this study using CD4/CD25/forkhead box protein P3 (FOXP3)/Helios markers.
Fifty participants, including 25 patients with secondary progressive MS (SPMS) and 25 healthy controls (HCs), were enrolled in this study, and their demographic characteristics were recorded. Peripheral blood samples ranging from 5 to 6 mL were obtained, and the Ficoll technique was used to extract peripheral blood mononuclear cells (PBMCs). Then, the percentage of CD4+CD25+FOXP3+Helios+ regulatory T lymphocytes was examined by flow cytometry in the study groups. Real-time polymerase chain reaction (PCR) was also used to assess the Helios gene expression level.
This study showed that the percentage of Tregs with CD4 and CD25 markers did not reveal a significant difference compared with HCs despite the decrease in SPMS patients (P = 0.6). However, lymphocytes with CD4/CD25/FOXP3/Helios markers were significantly reduced in the patients (P = 0.01). Additionally, SPMS patients had statistically significantly lower Helios gene expression levels (P = 0.002).
In SPMS patients, a decrease in the frequency of the CD4+CD25+FOXP3+Helios+ Treg population can result in an imbalanced immune system. In other words, one of the immunological mechanisms involved in this disease may be a deficiency in Tregs. Helios gene expression was also decreased in these patients, which may exacerbate functional defects in Tregs. |
| doi_str_mv | 10.1016/j.intimp.2023.109797 |
| format | Article |
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•Helios gene expression decreased in SPMS patients.•The percentage of Helios+ Tregs was low in the peripheral blood of SPMS patients.•One of the immunological mechanisms involved in SPMS may be a deficiency in Tregs.•The percentage of CD4+CD25+ T lymphocytes did not show a significant difference.
Multiple sclerosis (MS) is an aggressive disease characterized by central nervous system (CNS) inflammatory and demyelinating lesions. Tolerance failure is implicated in the development of several autoimmune disorders, including MS. Due to their involvement in maintaining environmental tolerance, regulatory T cells (Tregs) are regarded as efficient immune cells. We examined the frequency of Tregs in this study using CD4/CD25/forkhead box protein P3 (FOXP3)/Helios markers.
Fifty participants, including 25 patients with secondary progressive MS (SPMS) and 25 healthy controls (HCs), were enrolled in this study, and their demographic characteristics were recorded. Peripheral blood samples ranging from 5 to 6 mL were obtained, and the Ficoll technique was used to extract peripheral blood mononuclear cells (PBMCs). Then, the percentage of CD4+CD25+FOXP3+Helios+ regulatory T lymphocytes was examined by flow cytometry in the study groups. Real-time polymerase chain reaction (PCR) was also used to assess the Helios gene expression level.
This study showed that the percentage of Tregs with CD4 and CD25 markers did not reveal a significant difference compared with HCs despite the decrease in SPMS patients (P = 0.6). However, lymphocytes with CD4/CD25/FOXP3/Helios markers were significantly reduced in the patients (P = 0.01). Additionally, SPMS patients had statistically significantly lower Helios gene expression levels (P = 0.002).
In SPMS patients, a decrease in the frequency of the CD4+CD25+FOXP3+Helios+ Treg population can result in an imbalanced immune system. In other words, one of the immunological mechanisms involved in this disease may be a deficiency in Tregs. Helios gene expression was also decreased in these patients, which may exacerbate functional defects in Tregs.</description><identifier>ISSN: 1567-5769</identifier><identifier>EISSN: 1878-1705</identifier><identifier>DOI: 10.1016/j.intimp.2023.109797</identifier><identifier>PMID: 36738680</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Forkhead Transcription Factors - genetics ; Forkhead Transcription Factors - metabolism ; FOXP3 ; Gene Expression ; Helios ; Humans ; Leukocytes, Mononuclear - metabolism ; Lymphocyte ; Multiple sclerosis ; Multiple Sclerosis - genetics ; Multiple Sclerosis - metabolism ; Multiple Sclerosis, Chronic Progressive - genetics ; Multiple Sclerosis, Chronic Progressive - metabolism ; Regulatory T cells ; T-Lymphocytes, Regulatory</subject><ispartof>International immunopharmacology, 2023-03, Vol.116, p.109797-109797, Article 109797</ispartof><rights>2023 Elsevier B.V.</rights><rights>Copyright © 2023 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-4d2f8d06fd491ea3f8f7baf2ab2ed4a4d0bc1d6804673fb97267358cc96f94393</citedby><cites>FETCH-LOGICAL-c362t-4d2f8d06fd491ea3f8f7baf2ab2ed4a4d0bc1d6804673fb97267358cc96f94393</cites><orcidid>0000-0003-2083-7425</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.intimp.2023.109797$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36738680$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tarighi, Mona</creatorcontrib><creatorcontrib>Shahbazi, Mehdi</creatorcontrib><creatorcontrib>Saadat, Payam</creatorcontrib><creatorcontrib>Daraei, Abdolreza</creatorcontrib><creatorcontrib>Alizadeh khatir, Ali</creatorcontrib><creatorcontrib>Rahimifard, Kimiya</creatorcontrib><creatorcontrib>Mohammadnia-Afrouzi, Mousa</creatorcontrib><title>Decreased frequency of regulatory T cells and level of helios gene expression in secondary progressive multiple sclerosis patients: Evidence about the development of multiple sclerosis</title><title>International immunopharmacology</title><addtitle>Int Immunopharmacol</addtitle><description>[Display omitted]
•Helios gene expression decreased in SPMS patients.•The percentage of Helios+ Tregs was low in the peripheral blood of SPMS patients.•One of the immunological mechanisms involved in SPMS may be a deficiency in Tregs.•The percentage of CD4+CD25+ T lymphocytes did not show a significant difference.
Multiple sclerosis (MS) is an aggressive disease characterized by central nervous system (CNS) inflammatory and demyelinating lesions. Tolerance failure is implicated in the development of several autoimmune disorders, including MS. Due to their involvement in maintaining environmental tolerance, regulatory T cells (Tregs) are regarded as efficient immune cells. We examined the frequency of Tregs in this study using CD4/CD25/forkhead box protein P3 (FOXP3)/Helios markers.
Fifty participants, including 25 patients with secondary progressive MS (SPMS) and 25 healthy controls (HCs), were enrolled in this study, and their demographic characteristics were recorded. Peripheral blood samples ranging from 5 to 6 mL were obtained, and the Ficoll technique was used to extract peripheral blood mononuclear cells (PBMCs). Then, the percentage of CD4+CD25+FOXP3+Helios+ regulatory T lymphocytes was examined by flow cytometry in the study groups. Real-time polymerase chain reaction (PCR) was also used to assess the Helios gene expression level.
This study showed that the percentage of Tregs with CD4 and CD25 markers did not reveal a significant difference compared with HCs despite the decrease in SPMS patients (P = 0.6). However, lymphocytes with CD4/CD25/FOXP3/Helios markers were significantly reduced in the patients (P = 0.01). Additionally, SPMS patients had statistically significantly lower Helios gene expression levels (P = 0.002).
In SPMS patients, a decrease in the frequency of the CD4+CD25+FOXP3+Helios+ Treg population can result in an imbalanced immune system. In other words, one of the immunological mechanisms involved in this disease may be a deficiency in Tregs. Helios gene expression was also decreased in these patients, which may exacerbate functional defects in Tregs.</description><subject>Forkhead Transcription Factors - genetics</subject><subject>Forkhead Transcription Factors - metabolism</subject><subject>FOXP3</subject><subject>Gene Expression</subject><subject>Helios</subject><subject>Humans</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Lymphocyte</subject><subject>Multiple sclerosis</subject><subject>Multiple Sclerosis - genetics</subject><subject>Multiple Sclerosis - metabolism</subject><subject>Multiple Sclerosis, Chronic Progressive - genetics</subject><subject>Multiple Sclerosis, Chronic Progressive - metabolism</subject><subject>Regulatory T cells</subject><subject>T-Lymphocytes, Regulatory</subject><issn>1567-5769</issn><issn>1878-1705</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UcFu1TAQjBCIlsIfIOQjlzzsOLETDkiotIBUqZf2bDn2-tVPjh1s54n-GZ-H0xQulXpaa3fGsztTVe8J3hFM2KfDzvpsp3nX4IaW1sAH_qI6JT3va8Jx97K8O8brjrPhpHqT0gHj0m_J6-qEMk571uPT6s83UBFkAo1MhF8LeHWPgkER9ouTOcR7dIMUOJeQ9Bo5OIJb53fgbEhoDx4Q_J4jpGSDR9ajBCp4LQtxjmH_MDgCmhaX7ewAJeUghmQTmmW24HP6jC6OVhdhQHIMS0b5DpBehcI8FcAq95T-tnplpEvw7rGeVbeXFzfnP-qr6-8_z79e1YqyJtetbkyvMTO6HQhIanrDR2kaOTagW9lqPCqiixVtscSMA29K7XqlBmaGlg70rPq4_VuuKfakLCabVkOkh7Ak0XBOCaEMdwXablBVNkwRjJijnYoTgmCxZiYOYstMrJmJLbNC-_CosIwT6P-kfyEVwJcNAOXOo4UokrKrX9pGUFnoYJ9X-AthkbAo</recordid><startdate>202303</startdate><enddate>202303</enddate><creator>Tarighi, Mona</creator><creator>Shahbazi, Mehdi</creator><creator>Saadat, Payam</creator><creator>Daraei, Abdolreza</creator><creator>Alizadeh khatir, Ali</creator><creator>Rahimifard, Kimiya</creator><creator>Mohammadnia-Afrouzi, Mousa</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2083-7425</orcidid></search><sort><creationdate>202303</creationdate><title>Decreased frequency of regulatory T cells and level of helios gene expression in secondary progressive multiple sclerosis patients: Evidence about the development of multiple sclerosis</title><author>Tarighi, Mona ; Shahbazi, Mehdi ; Saadat, Payam ; Daraei, Abdolreza ; Alizadeh khatir, Ali ; Rahimifard, Kimiya ; Mohammadnia-Afrouzi, Mousa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-4d2f8d06fd491ea3f8f7baf2ab2ed4a4d0bc1d6804673fb97267358cc96f94393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Forkhead Transcription Factors - genetics</topic><topic>Forkhead Transcription Factors - metabolism</topic><topic>FOXP3</topic><topic>Gene Expression</topic><topic>Helios</topic><topic>Humans</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Lymphocyte</topic><topic>Multiple sclerosis</topic><topic>Multiple Sclerosis - genetics</topic><topic>Multiple Sclerosis - metabolism</topic><topic>Multiple Sclerosis, Chronic Progressive - genetics</topic><topic>Multiple Sclerosis, Chronic Progressive - metabolism</topic><topic>Regulatory T cells</topic><topic>T-Lymphocytes, Regulatory</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tarighi, Mona</creatorcontrib><creatorcontrib>Shahbazi, Mehdi</creatorcontrib><creatorcontrib>Saadat, Payam</creatorcontrib><creatorcontrib>Daraei, Abdolreza</creatorcontrib><creatorcontrib>Alizadeh khatir, Ali</creatorcontrib><creatorcontrib>Rahimifard, Kimiya</creatorcontrib><creatorcontrib>Mohammadnia-Afrouzi, Mousa</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International immunopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tarighi, Mona</au><au>Shahbazi, Mehdi</au><au>Saadat, Payam</au><au>Daraei, Abdolreza</au><au>Alizadeh khatir, Ali</au><au>Rahimifard, Kimiya</au><au>Mohammadnia-Afrouzi, Mousa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decreased frequency of regulatory T cells and level of helios gene expression in secondary progressive multiple sclerosis patients: Evidence about the development of multiple sclerosis</atitle><jtitle>International immunopharmacology</jtitle><addtitle>Int Immunopharmacol</addtitle><date>2023-03</date><risdate>2023</risdate><volume>116</volume><spage>109797</spage><epage>109797</epage><pages>109797-109797</pages><artnum>109797</artnum><issn>1567-5769</issn><eissn>1878-1705</eissn><abstract>[Display omitted]
•Helios gene expression decreased in SPMS patients.•The percentage of Helios+ Tregs was low in the peripheral blood of SPMS patients.•One of the immunological mechanisms involved in SPMS may be a deficiency in Tregs.•The percentage of CD4+CD25+ T lymphocytes did not show a significant difference.
Multiple sclerosis (MS) is an aggressive disease characterized by central nervous system (CNS) inflammatory and demyelinating lesions. Tolerance failure is implicated in the development of several autoimmune disorders, including MS. Due to their involvement in maintaining environmental tolerance, regulatory T cells (Tregs) are regarded as efficient immune cells. We examined the frequency of Tregs in this study using CD4/CD25/forkhead box protein P3 (FOXP3)/Helios markers.
Fifty participants, including 25 patients with secondary progressive MS (SPMS) and 25 healthy controls (HCs), were enrolled in this study, and their demographic characteristics were recorded. Peripheral blood samples ranging from 5 to 6 mL were obtained, and the Ficoll technique was used to extract peripheral blood mononuclear cells (PBMCs). Then, the percentage of CD4+CD25+FOXP3+Helios+ regulatory T lymphocytes was examined by flow cytometry in the study groups. Real-time polymerase chain reaction (PCR) was also used to assess the Helios gene expression level.
This study showed that the percentage of Tregs with CD4 and CD25 markers did not reveal a significant difference compared with HCs despite the decrease in SPMS patients (P = 0.6). However, lymphocytes with CD4/CD25/FOXP3/Helios markers were significantly reduced in the patients (P = 0.01). Additionally, SPMS patients had statistically significantly lower Helios gene expression levels (P = 0.002).
In SPMS patients, a decrease in the frequency of the CD4+CD25+FOXP3+Helios+ Treg population can result in an imbalanced immune system. In other words, one of the immunological mechanisms involved in this disease may be a deficiency in Tregs. Helios gene expression was also decreased in these patients, which may exacerbate functional defects in Tregs.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>36738680</pmid><doi>10.1016/j.intimp.2023.109797</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-2083-7425</orcidid></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Forkhead Transcription Factors - genetics Forkhead Transcription Factors - metabolism FOXP3 Gene Expression Helios Humans Leukocytes, Mononuclear - metabolism Lymphocyte Multiple sclerosis Multiple Sclerosis - genetics Multiple Sclerosis - metabolism Multiple Sclerosis, Chronic Progressive - genetics Multiple Sclerosis, Chronic Progressive - metabolism Regulatory T cells T-Lymphocytes, Regulatory |
| title | Decreased frequency of regulatory T cells and level of helios gene expression in secondary progressive multiple sclerosis patients: Evidence about the development of multiple sclerosis |
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