Oligofructose, 2'fucosyllactose and β-glucan in combination induce specific changes in microbial composition and short-chain fatty acid production compared to sole supplementation
In this study, we explored the effects that the prebiotic inulin-type fructans, and prebiotic candidates: 2'fucosyllactose and β-glucan from barley, singular and in combination had on microbial load, microbiome profile, and short-chain fatty acid production. This was carried out as a prescreeni...
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| Veröffentlicht in: | Journal of applied microbiology 2023-02, Vol.134 (2) |
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| creator | Jackson, Peter Philip James Wijeyesekera, Anisha van Harsselaar, Jessica Theis, Stephan Rastall, Robert Adrian |
| description | In this study, we explored the effects that the prebiotic inulin-type fructans, and prebiotic candidates: 2'fucosyllactose and β-glucan from barley, singular and in combination had on microbial load, microbiome profile, and short-chain fatty acid production. This was carried out as a prescreening tool to determine combinations that could be taken forward for use in a human intervention trial.
Effects of inulin-type fructans, 2'fucosyllactose and β-glucan from barley in singular and combination on microbial load and profile and short-chain fatty acid production (SCFA) was conducted using in vitro batch culture fermentation over 48 h. Changes in microbial load and profile were assessed by fluorescence in situ hybridization flow cytometry (FISH-FLOW) and 16S rRNA sequencing, and changes in SCFA via gas chromatography. All substrates generated changes in microbial load and profile, achieving peak microbial load at 8 h fermentation with the largest changes in profile across all substrates in Bifidobacterium (Q |
| doi_str_mv | 10.1093/jambio/lxac069 |
| format | Article |
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Effects of inulin-type fructans, 2'fucosyllactose and β-glucan from barley in singular and combination on microbial load and profile and short-chain fatty acid production (SCFA) was conducted using in vitro batch culture fermentation over 48 h. Changes in microbial load and profile were assessed by fluorescence in situ hybridization flow cytometry (FISH-FLOW) and 16S rRNA sequencing, and changes in SCFA via gas chromatography. All substrates generated changes in microbial load and profile, achieving peak microbial load at 8 h fermentation with the largest changes in profile across all substrates in Bifidobacterium (Q < 0.05). This coincided with significant increases in acetate observed throughout fermentation (Q < 0.05). In comparison to sole supplementation combinations of oligofructose, β-glucan and 2'fuscosyllactose induced significant increases in both propionate and butyrate producing bacteria (Roseburia and Faecalibacterium praunitzii), and concentrations of propionate and butyrate, the latter being maintained until the end of fermentation (all Q < 0.05).
Combinations of oligofructose, with β-glucan and 2'fucosyllactose induced selective changes in microbial combination and SCFA namely Roseburia, F. praunitzii, propionate and butyrate compared to sole supplementation.</description><identifier>ISSN: 1365-2672</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1093/jambio/lxac069</identifier><identifier>PMID: 36724263</identifier><language>eng</language><publisher>England</publisher><subject>beta-Glucans ; Butyrates ; Fatty Acids, Volatile ; Feces - microbiology ; Fermentation ; Fructans - pharmacology ; Hordeum - genetics ; Hordeum - metabolism ; Humans ; In Situ Hybridization, Fluorescence ; Inulin - metabolism ; Inulin - pharmacology ; Prebiotics ; Propionates ; RNA, Ribosomal, 16S - genetics</subject><ispartof>Journal of applied microbiology, 2023-02, Vol.134 (2)</ispartof><rights>The Author(s) 2022. Published by Oxford University Press on behalf of Applied Microbiology International.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c335t-60d3361f456f6c6e2cb728c026f92cd4b7efc9dc881ad1b93025b4c25919ecab3</citedby><cites>FETCH-LOGICAL-c335t-60d3361f456f6c6e2cb728c026f92cd4b7efc9dc881ad1b93025b4c25919ecab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36724263$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jackson, Peter Philip James</creatorcontrib><creatorcontrib>Wijeyesekera, Anisha</creatorcontrib><creatorcontrib>van Harsselaar, Jessica</creatorcontrib><creatorcontrib>Theis, Stephan</creatorcontrib><creatorcontrib>Rastall, Robert Adrian</creatorcontrib><title>Oligofructose, 2'fucosyllactose and β-glucan in combination induce specific changes in microbial composition and short-chain fatty acid production compared to sole supplementation</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>In this study, we explored the effects that the prebiotic inulin-type fructans, and prebiotic candidates: 2'fucosyllactose and β-glucan from barley, singular and in combination had on microbial load, microbiome profile, and short-chain fatty acid production. This was carried out as a prescreening tool to determine combinations that could be taken forward for use in a human intervention trial.
Effects of inulin-type fructans, 2'fucosyllactose and β-glucan from barley in singular and combination on microbial load and profile and short-chain fatty acid production (SCFA) was conducted using in vitro batch culture fermentation over 48 h. Changes in microbial load and profile were assessed by fluorescence in situ hybridization flow cytometry (FISH-FLOW) and 16S rRNA sequencing, and changes in SCFA via gas chromatography. All substrates generated changes in microbial load and profile, achieving peak microbial load at 8 h fermentation with the largest changes in profile across all substrates in Bifidobacterium (Q < 0.05). This coincided with significant increases in acetate observed throughout fermentation (Q < 0.05). In comparison to sole supplementation combinations of oligofructose, β-glucan and 2'fuscosyllactose induced significant increases in both propionate and butyrate producing bacteria (Roseburia and Faecalibacterium praunitzii), and concentrations of propionate and butyrate, the latter being maintained until the end of fermentation (all Q < 0.05).
Combinations of oligofructose, with β-glucan and 2'fucosyllactose induced selective changes in microbial combination and SCFA namely Roseburia, F. praunitzii, propionate and butyrate compared to sole supplementation.</description><subject>beta-Glucans</subject><subject>Butyrates</subject><subject>Fatty Acids, Volatile</subject><subject>Feces - microbiology</subject><subject>Fermentation</subject><subject>Fructans - pharmacology</subject><subject>Hordeum - genetics</subject><subject>Hordeum - metabolism</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Inulin - metabolism</subject><subject>Inulin - pharmacology</subject><subject>Prebiotics</subject><subject>Propionates</subject><subject>RNA, Ribosomal, 16S - genetics</subject><issn>1365-2672</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkc1u3CAUhVGVqDNJu-2yYpcu4gk_Nh6W1ahJK0WaTbK28AUmRNi4Bkud14r6HHmm4plplBVw9Z1zgIPQF0pWlEh-86y61oUb_0cBEfIDWlIuqoKJmp292y_QRYzPhFBOKvERLXielUzwJfq79W4X7DhBCtFcY3ZlJwhx7706TLDqNX59KXZ-AtVj12MIObFXyYX5qCcwOA4GnHWA4Un1OxNnrHMwhtYpPwuGEN1BMLvFpzCmIqOZsiqlPVbgNB7GkM0O1KxQo9E4BRyDzwHTMHjTmT4dcj-hc6t8NJ9P6yV6vP3xsPlZ3G_vfm2-3xfAeZUKQTTngtqyElaAMAzamq2BMGElA122tbEgNazXVGnaSk5Y1ZbAKkmlAdXyS_Tt6Jvv9nsyMTWdi2Dy3_QmTLFhdU1lyZisMro6ovnVMY7GNsPoOjXuG0qauanm2FRzaioLvp68p7Yz-g3_Xw3_B_83mA0</recordid><startdate>20230216</startdate><enddate>20230216</enddate><creator>Jackson, Peter Philip James</creator><creator>Wijeyesekera, Anisha</creator><creator>van Harsselaar, Jessica</creator><creator>Theis, Stephan</creator><creator>Rastall, Robert Adrian</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20230216</creationdate><title>Oligofructose, 2'fucosyllactose and β-glucan in combination induce specific changes in microbial composition and short-chain fatty acid production compared to sole supplementation</title><author>Jackson, Peter Philip James ; Wijeyesekera, Anisha ; van Harsselaar, Jessica ; Theis, Stephan ; Rastall, Robert Adrian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c335t-60d3361f456f6c6e2cb728c026f92cd4b7efc9dc881ad1b93025b4c25919ecab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>beta-Glucans</topic><topic>Butyrates</topic><topic>Fatty Acids, Volatile</topic><topic>Feces - microbiology</topic><topic>Fermentation</topic><topic>Fructans - pharmacology</topic><topic>Hordeum - genetics</topic><topic>Hordeum - metabolism</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Inulin - metabolism</topic><topic>Inulin - pharmacology</topic><topic>Prebiotics</topic><topic>Propionates</topic><topic>RNA, Ribosomal, 16S - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jackson, Peter Philip James</creatorcontrib><creatorcontrib>Wijeyesekera, Anisha</creatorcontrib><creatorcontrib>van Harsselaar, Jessica</creatorcontrib><creatorcontrib>Theis, Stephan</creatorcontrib><creatorcontrib>Rastall, Robert Adrian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jackson, Peter Philip James</au><au>Wijeyesekera, Anisha</au><au>van Harsselaar, Jessica</au><au>Theis, Stephan</au><au>Rastall, Robert Adrian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oligofructose, 2'fucosyllactose and β-glucan in combination induce specific changes in microbial composition and short-chain fatty acid production compared to sole supplementation</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2023-02-16</date><risdate>2023</risdate><volume>134</volume><issue>2</issue><issn>1365-2672</issn><eissn>1365-2672</eissn><abstract>In this study, we explored the effects that the prebiotic inulin-type fructans, and prebiotic candidates: 2'fucosyllactose and β-glucan from barley, singular and in combination had on microbial load, microbiome profile, and short-chain fatty acid production. This was carried out as a prescreening tool to determine combinations that could be taken forward for use in a human intervention trial.
Effects of inulin-type fructans, 2'fucosyllactose and β-glucan from barley in singular and combination on microbial load and profile and short-chain fatty acid production (SCFA) was conducted using in vitro batch culture fermentation over 48 h. Changes in microbial load and profile were assessed by fluorescence in situ hybridization flow cytometry (FISH-FLOW) and 16S rRNA sequencing, and changes in SCFA via gas chromatography. All substrates generated changes in microbial load and profile, achieving peak microbial load at 8 h fermentation with the largest changes in profile across all substrates in Bifidobacterium (Q < 0.05). This coincided with significant increases in acetate observed throughout fermentation (Q < 0.05). In comparison to sole supplementation combinations of oligofructose, β-glucan and 2'fuscosyllactose induced significant increases in both propionate and butyrate producing bacteria (Roseburia and Faecalibacterium praunitzii), and concentrations of propionate and butyrate, the latter being maintained until the end of fermentation (all Q < 0.05).
Combinations of oligofructose, with β-glucan and 2'fucosyllactose induced selective changes in microbial combination and SCFA namely Roseburia, F. praunitzii, propionate and butyrate compared to sole supplementation.</abstract><cop>England</cop><pmid>36724263</pmid><doi>10.1093/jambio/lxac069</doi><oa>free_for_read</oa></addata></record> |
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| subjects | beta-Glucans Butyrates Fatty Acids, Volatile Feces - microbiology Fermentation Fructans - pharmacology Hordeum - genetics Hordeum - metabolism Humans In Situ Hybridization, Fluorescence Inulin - metabolism Inulin - pharmacology Prebiotics Propionates RNA, Ribosomal, 16S - genetics |
| title | Oligofructose, 2'fucosyllactose and β-glucan in combination induce specific changes in microbial composition and short-chain fatty acid production compared to sole supplementation |
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