Use of the Comet assay to assess DNA damage in hemocytes and digestive gland cells of mussels and clams exposed to water contaminated with petroleum hydrocarbons
Oil spills can result in the deposition of large quantities of petroleum hydrocarbons into intertidal and shallow waters seriously impacting bivalve populations. Petroleum hydrocarbons are enriched in polycyclic aromatic hydrocarbons (PAH) and PAH analogs many of which may have potential to damage D...
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| Veröffentlicht in: | Marine environmental research 2002-09, Vol.54 (3-5), p.471-474 |
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| creator | Hamoutene, D Payne, J.F Rahimtula, A Lee, K |
| description | Oil spills can result in the deposition of large quantities of petroleum hydrocarbons into intertidal and shallow waters seriously impacting bivalve populations. Petroleum hydrocarbons are enriched in polycyclic aromatic hydrocarbons (PAH) and PAH analogs many of which may have potential to damage DNA. The Comet assay is useful for assessing DNA damage and has been used to a limited degree with aquatic organisms, but mostly with studies in vitro. We have carried out studies with the Comet assay to assess the DNA damaging potential of complex mixtures of petroleum hydrocarbons for bivalves. Experiments were carried out with mussels (Mytilus edulis) and clams (Mya arenaria) with dispersions and water soluble fractions of an Arabian crude oil which was also chemically characterized in detail by GC–MS. Pilot studies were first conducted to evaluate test performance and reproducibility. An interindividual coefficient of variation ranging from 17 to 30% was established for the assay with hemocytes and digestive gland cells of both species. Exposure to hydrocarbon fractions had no significant impact on clams. However, an increase in DNA damage was observed at P |
| doi_str_mv | 10.1016/S0141-1136(02)00162-9 |
| format | Article |
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Petroleum hydrocarbons are enriched in polycyclic aromatic hydrocarbons (PAH) and PAH analogs many of which may have potential to damage DNA. The Comet assay is useful for assessing DNA damage and has been used to a limited degree with aquatic organisms, but mostly with studies in vitro. We have carried out studies with the Comet assay to assess the DNA damaging potential of complex mixtures of petroleum hydrocarbons for bivalves. Experiments were carried out with mussels (Mytilus edulis) and clams (Mya arenaria) with dispersions and water soluble fractions of an Arabian crude oil which was also chemically characterized in detail by GC–MS. Pilot studies were first conducted to evaluate test performance and reproducibility. An interindividual coefficient of variation ranging from 17 to 30% was established for the assay with hemocytes and digestive gland cells of both species. Exposure to hydrocarbon fractions had no significant impact on clams. However, an increase in DNA damage was observed at P<0.1 with digestive gland cells of mussels exposed to aqueous fractions of a light crude oil. These studies have demonstrated a potential for DNA damage in bivalves exposed to oil spills in inshore waters as well as potential for interspecies sensitivity.</description><identifier>ISSN: 0141-1136</identifier><identifier>EISSN: 1879-0291</identifier><identifier>DOI: 10.1016/S0141-1136(02)00162-9</identifier><identifier>PMID: 12408603</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animal, plant and microbial ecology ; Animals ; Applied ecology ; Biological and medical sciences ; Bivalves ; Bivalvia ; Bivalvia - genetics ; Bivalvia - physiology ; Comet Assay ; Digestive System - cytology ; DNA ; DNA Damage ; Ecotoxicology, biological effects of pollution ; Effects of pollution and side effects of pesticides on protozoa and invertebrates ; Environmental Exposure ; Fundamental and applied biological sciences. Psychology ; Gas Chromatography-Mass Spectrometry ; Hemocytes ; Hydrocarbons ; Marine ; Mya arenaria ; Mytilus edulis ; Petroleum ; Petroleum - adverse effects ; Reproducibility of Results ; Tissue Distribution ; Water Pollutants, Chemical - adverse effects</subject><ispartof>Marine environmental research, 2002-09, Vol.54 (3-5), p.471-474</ispartof><rights>2002 Elsevier Science Ltd</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-9298f6ed1fbe6c8f6d2c2320608b0224850c972a341ffd84c252946b12ccc2263</citedby><cites>FETCH-LOGICAL-c484t-9298f6ed1fbe6c8f6d2c2320608b0224850c972a341ffd84c252946b12ccc2263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0141113602001629$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,3537,23909,23910,25118,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13939445$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12408603$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hamoutene, D</creatorcontrib><creatorcontrib>Payne, J.F</creatorcontrib><creatorcontrib>Rahimtula, A</creatorcontrib><creatorcontrib>Lee, K</creatorcontrib><title>Use of the Comet assay to assess DNA damage in hemocytes and digestive gland cells of mussels and clams exposed to water contaminated with petroleum hydrocarbons</title><title>Marine environmental research</title><addtitle>Mar Environ Res</addtitle><description>Oil spills can result in the deposition of large quantities of petroleum hydrocarbons into intertidal and shallow waters seriously impacting bivalve populations. Petroleum hydrocarbons are enriched in polycyclic aromatic hydrocarbons (PAH) and PAH analogs many of which may have potential to damage DNA. The Comet assay is useful for assessing DNA damage and has been used to a limited degree with aquatic organisms, but mostly with studies in vitro. We have carried out studies with the Comet assay to assess the DNA damaging potential of complex mixtures of petroleum hydrocarbons for bivalves. Experiments were carried out with mussels (Mytilus edulis) and clams (Mya arenaria) with dispersions and water soluble fractions of an Arabian crude oil which was also chemically characterized in detail by GC–MS. Pilot studies were first conducted to evaluate test performance and reproducibility. An interindividual coefficient of variation ranging from 17 to 30% was established for the assay with hemocytes and digestive gland cells of both species. Exposure to hydrocarbon fractions had no significant impact on clams. However, an increase in DNA damage was observed at P<0.1 with digestive gland cells of mussels exposed to aqueous fractions of a light crude oil. These studies have demonstrated a potential for DNA damage in bivalves exposed to oil spills in inshore waters as well as potential for interspecies sensitivity.</description><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Applied ecology</subject><subject>Biological and medical sciences</subject><subject>Bivalves</subject><subject>Bivalvia</subject><subject>Bivalvia - genetics</subject><subject>Bivalvia - physiology</subject><subject>Comet Assay</subject><subject>Digestive System - cytology</subject><subject>DNA</subject><subject>DNA Damage</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Effects of pollution and side effects of pesticides on protozoa and invertebrates</subject><subject>Environmental Exposure</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Hemocytes</subject><subject>Hydrocarbons</subject><subject>Marine</subject><subject>Mya arenaria</subject><subject>Mytilus edulis</subject><subject>Petroleum</subject><subject>Petroleum - adverse effects</subject><subject>Reproducibility of Results</subject><subject>Tissue Distribution</subject><subject>Water Pollutants, Chemical - adverse effects</subject><issn>0141-1136</issn><issn>1879-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhiMEokvhEUC-gOghYE8cJz6haqGAVMEBera89mTXKIkXj7dlH4c3Jemu6HFPnrG-_58Z_UXxUvB3ggv1_gcXUpRCVOothws-fUGpHxUL0Ta65KDF42LxHzkrnhH94pzXjaifFmcCJG8VrxbF3xtCFjuWN8iWccDMLJHdsxznAonYx2-XzNvBrpGFkW1wiG6fkZgdPfNhjZTDLbJ1P_cO-55mu2E3ifsD5Ho7EMM_20joZ-M7mzExF8dshzBOjWd3IW_YFnOKPe4Gttn7FJ1NqzjS8-JJZ3vCF8f3vLi5-vRz-aW8_v756_LyunSylbnUoNtOoRfdCpWbSg8OKuCKtysOINuaO92AraToOt9KBzVoqVYCnHMAqjov3hx8tyn-3k1nmSHQfJAdMe7IQKO0AC5OgkLXDahGngalqhRUegLrA-hSJErYmW0Kg017I7iZ0zb3aZs5SsPB3KdtZt2r44DdakD_oDrGOwGvj4AlZ_su2dEFeuCm2VrKeuI-HLgpM7wNmAy5gKNDHxK6bHwMJ1b5B8Nnx8c</recordid><startdate>20020901</startdate><enddate>20020901</enddate><creator>Hamoutene, D</creator><creator>Payne, J.F</creator><creator>Rahimtula, A</creator><creator>Lee, K</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7QH</scope><scope>7TN</scope><scope>7TV</scope><scope>7U7</scope><scope>7UA</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>8FD</scope><scope>FR3</scope><scope>KR7</scope></search><sort><creationdate>20020901</creationdate><title>Use of the Comet assay to assess DNA damage in hemocytes and digestive gland cells of mussels and clams exposed to water contaminated with petroleum hydrocarbons</title><author>Hamoutene, D ; Payne, J.F ; Rahimtula, A ; Lee, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-9298f6ed1fbe6c8f6d2c2320608b0224850c972a341ffd84c252946b12ccc2263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Applied ecology</topic><topic>Biological and medical sciences</topic><topic>Bivalves</topic><topic>Bivalvia</topic><topic>Bivalvia - genetics</topic><topic>Bivalvia - physiology</topic><topic>Comet Assay</topic><topic>Digestive System - cytology</topic><topic>DNA</topic><topic>DNA Damage</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Effects of pollution and side effects of pesticides on protozoa and invertebrates</topic><topic>Environmental Exposure</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Hemocytes</topic><topic>Hydrocarbons</topic><topic>Marine</topic><topic>Mya arenaria</topic><topic>Mytilus edulis</topic><topic>Petroleum</topic><topic>Petroleum - adverse effects</topic><topic>Reproducibility of Results</topic><topic>Tissue Distribution</topic><topic>Water Pollutants, Chemical - adverse effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hamoutene, D</creatorcontrib><creatorcontrib>Payne, J.F</creatorcontrib><creatorcontrib>Rahimtula, A</creatorcontrib><creatorcontrib>Lee, K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Aqualine</collection><collection>Oceanic Abstracts</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><jtitle>Marine environmental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hamoutene, D</au><au>Payne, J.F</au><au>Rahimtula, A</au><au>Lee, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of the Comet assay to assess DNA damage in hemocytes and digestive gland cells of mussels and clams exposed to water contaminated with petroleum hydrocarbons</atitle><jtitle>Marine environmental research</jtitle><addtitle>Mar Environ Res</addtitle><date>2002-09-01</date><risdate>2002</risdate><volume>54</volume><issue>3-5</issue><spage>471</spage><epage>474</epage><pages>471-474</pages><issn>0141-1136</issn><eissn>1879-0291</eissn><abstract>Oil spills can result in the deposition of large quantities of petroleum hydrocarbons into intertidal and shallow waters seriously impacting bivalve populations. Petroleum hydrocarbons are enriched in polycyclic aromatic hydrocarbons (PAH) and PAH analogs many of which may have potential to damage DNA. The Comet assay is useful for assessing DNA damage and has been used to a limited degree with aquatic organisms, but mostly with studies in vitro. We have carried out studies with the Comet assay to assess the DNA damaging potential of complex mixtures of petroleum hydrocarbons for bivalves. Experiments were carried out with mussels (Mytilus edulis) and clams (Mya arenaria) with dispersions and water soluble fractions of an Arabian crude oil which was also chemically characterized in detail by GC–MS. Pilot studies were first conducted to evaluate test performance and reproducibility. An interindividual coefficient of variation ranging from 17 to 30% was established for the assay with hemocytes and digestive gland cells of both species. Exposure to hydrocarbon fractions had no significant impact on clams. 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| ispartof | Marine environmental research, 2002-09, Vol.54 (3-5), p.471-474 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animal, plant and microbial ecology Animals Applied ecology Biological and medical sciences Bivalves Bivalvia Bivalvia - genetics Bivalvia - physiology Comet Assay Digestive System - cytology DNA DNA Damage Ecotoxicology, biological effects of pollution Effects of pollution and side effects of pesticides on protozoa and invertebrates Environmental Exposure Fundamental and applied biological sciences. Psychology Gas Chromatography-Mass Spectrometry Hemocytes Hydrocarbons Marine Mya arenaria Mytilus edulis Petroleum Petroleum - adverse effects Reproducibility of Results Tissue Distribution Water Pollutants, Chemical - adverse effects |
| title | Use of the Comet assay to assess DNA damage in hemocytes and digestive gland cells of mussels and clams exposed to water contaminated with petroleum hydrocarbons |
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