Drought‐induced CsMYB6 interacts with CsbHLH111 to regulate anthocyanin biosynthesis in Chaenomeles speciosa
Chaenomeles speciosa is a plant with high ornamental value, and the color of its petals deepens obviously under drought stress. To understand the mechanism of drought‐induced reddening of C. speciosa petal color, the metabolites and transcriptomics of petals from 4% PEG‐8000‐treated and control cutt...
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| Veröffentlicht in: | Physiologia plantarum 2023-01, Vol.175 (1), p.e13859-n/a |
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| creator | Zhang, Shuangyu Ren, Yanshen Zhao, Qianyi Wu, Yang Zhuo, Yue Li, Houhua |
| description | Chaenomeles speciosa is a plant with high ornamental value, and the color of its petals deepens obviously under drought stress. To understand the mechanism of drought‐induced reddening of C. speciosa petal color, the metabolites and transcriptomics of petals from 4% PEG‐8000‐treated and control cuttings were analyzed. In this study, the analysis of metabolites revealed the accumulation of anthocyanins in petals of PEG‐treated cuttings, indicating anthocyanins might be the reason for the deepening of petal color. By using transcriptomics, we identified CsMYB6 as an overexpressed transcription factor in PEG‐treated samples. Transient overexpression and suppression of CsMYB6 revealed that it is a key transcription factor for anthocyanin synthesis. We identified genes related to anthocyanin biosynthesis and constructed a network of drought‐ and anthocyanin‐related genes (such as CsMYB6, CsbHLH111, CsANS, CsDFR, and CsUFGT). Further experiments indicated that CsMYB6 directly interacted with CsbHLH111, and this interaction increased the binding ability of CsMYB6 to the promoter regions of three structural genes of anthocyanin biosynthesis: CsANS, CsDFR, and CsUFGT. Our findings provide a molecular basis and new insight into drought‐induced anthocyanin biosynthesis in C. speciosa. |
| doi_str_mv | 10.1111/ppl.13859 |
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To understand the mechanism of drought‐induced reddening of C. speciosa petal color, the metabolites and transcriptomics of petals from 4% PEG‐8000‐treated and control cuttings were analyzed. In this study, the analysis of metabolites revealed the accumulation of anthocyanins in petals of PEG‐treated cuttings, indicating anthocyanins might be the reason for the deepening of petal color. By using transcriptomics, we identified CsMYB6 as an overexpressed transcription factor in PEG‐treated samples. Transient overexpression and suppression of CsMYB6 revealed that it is a key transcription factor for anthocyanin synthesis. We identified genes related to anthocyanin biosynthesis and constructed a network of drought‐ and anthocyanin‐related genes (such as CsMYB6, CsbHLH111, CsANS, CsDFR, and CsUFGT). Further experiments indicated that CsMYB6 directly interacted with CsbHLH111, and this interaction increased the binding ability of CsMYB6 to the promoter regions of three structural genes of anthocyanin biosynthesis: CsANS, CsDFR, and CsUFGT. Our findings provide a molecular basis and new insight into drought‐induced anthocyanin biosynthesis in C. speciosa.</description><identifier>ISSN: 0031-9317</identifier><identifier>EISSN: 1399-3054</identifier><identifier>DOI: 10.1111/ppl.13859</identifier><identifier>PMID: 36688571</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Anthocyanins ; Anthocyanins - metabolism ; Biosynthesis ; Chaenomeles speciosa ; Color ; Cuttings ; Drought ; Droughts ; Flowers - genetics ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Genes ; Metabolites ; Ornamental plants ; Petals ; Plant Proteins - metabolism ; Rosaceae - genetics ; Rosaceae - metabolism ; Transcription factors ; Transcription Factors - metabolism ; Transcriptomics</subject><ispartof>Physiologia plantarum, 2023-01, Vol.175 (1), p.e13859-n/a</ispartof><rights>2023 Scandinavian Plant Physiology Society.</rights><rights>2023 Scandinavian Plant Physiology Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2839-923f26da022291903effd26277ce4af6b866c92ff3d72fbf763b9a31b2397a2f3</citedby><cites>FETCH-LOGICAL-c2839-923f26da022291903effd26277ce4af6b866c92ff3d72fbf763b9a31b2397a2f3</cites><orcidid>0000-0001-5247-5508</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fppl.13859$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fppl.13859$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36688571$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Shuangyu</creatorcontrib><creatorcontrib>Ren, Yanshen</creatorcontrib><creatorcontrib>Zhao, Qianyi</creatorcontrib><creatorcontrib>Wu, Yang</creatorcontrib><creatorcontrib>Zhuo, Yue</creatorcontrib><creatorcontrib>Li, Houhua</creatorcontrib><title>Drought‐induced CsMYB6 interacts with CsbHLH111 to regulate anthocyanin biosynthesis in Chaenomeles speciosa</title><title>Physiologia plantarum</title><addtitle>Physiol Plant</addtitle><description>Chaenomeles speciosa is a plant with high ornamental value, and the color of its petals deepens obviously under drought stress. To understand the mechanism of drought‐induced reddening of C. speciosa petal color, the metabolites and transcriptomics of petals from 4% PEG‐8000‐treated and control cuttings were analyzed. In this study, the analysis of metabolites revealed the accumulation of anthocyanins in petals of PEG‐treated cuttings, indicating anthocyanins might be the reason for the deepening of petal color. By using transcriptomics, we identified CsMYB6 as an overexpressed transcription factor in PEG‐treated samples. Transient overexpression and suppression of CsMYB6 revealed that it is a key transcription factor for anthocyanin synthesis. We identified genes related to anthocyanin biosynthesis and constructed a network of drought‐ and anthocyanin‐related genes (such as CsMYB6, CsbHLH111, CsANS, CsDFR, and CsUFGT). Further experiments indicated that CsMYB6 directly interacted with CsbHLH111, and this interaction increased the binding ability of CsMYB6 to the promoter regions of three structural genes of anthocyanin biosynthesis: CsANS, CsDFR, and CsUFGT. Our findings provide a molecular basis and new insight into drought‐induced anthocyanin biosynthesis in C. speciosa.</description><subject>Anthocyanins</subject><subject>Anthocyanins - metabolism</subject><subject>Biosynthesis</subject><subject>Chaenomeles speciosa</subject><subject>Color</subject><subject>Cuttings</subject><subject>Drought</subject><subject>Droughts</subject><subject>Flowers - genetics</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Metabolites</subject><subject>Ornamental plants</subject><subject>Petals</subject><subject>Plant Proteins - metabolism</subject><subject>Rosaceae - genetics</subject><subject>Rosaceae - metabolism</subject><subject>Transcription factors</subject><subject>Transcription Factors - metabolism</subject><subject>Transcriptomics</subject><issn>0031-9317</issn><issn>1399-3054</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1OGzEUha0K1KShi75AZYlNWQz4h9jjZUlpg5SqWcCC1cjjuSaOJvbUnlGUHY_AM_IkuAS6QOJurHv9-VzrHIS-UHJKc511XXtKeTlVH9CYcqUKTqbnB2hMCKeF4lSO0KeU1oRQISj7iEZciLKcSjpG_kcMw92qf7x_cL4ZDDR4ln7fXgjsfA9Rmz7hretXeVrPF_O8DfcBR7gbWt0D1r5fBbPT3nlcu5B2uYfkUn6NZysNPmyghYRTByZf6yN0aHWb4PPLOUE3Py-vZ_Ni8efX1ez7ojCs5KpQjFsmGk0YY4oqwsHahgkmpYFzbUVdCmEUs5Y3ktnaSsFrpTmtGVdSM8sn6Ntet4vh7wCprzYuGWhb7SEMqWIyG0C5mNKMHr9B12GIPv8uUzLbxCkpM3Wyp0wMKUWwVRfdRsddRUn1L4Qqh1A9h5DZry-KQ72B5j_56noGzvbA1rWwe1-pWi4Xe8kntyqRqg</recordid><startdate>202301</startdate><enddate>202301</enddate><creator>Zhang, Shuangyu</creator><creator>Ren, Yanshen</creator><creator>Zhao, Qianyi</creator><creator>Wu, Yang</creator><creator>Zhuo, Yue</creator><creator>Li, Houhua</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SN</scope><scope>7ST</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5247-5508</orcidid></search><sort><creationdate>202301</creationdate><title>Drought‐induced CsMYB6 interacts with CsbHLH111 to regulate anthocyanin biosynthesis in Chaenomeles speciosa</title><author>Zhang, Shuangyu ; Ren, Yanshen ; Zhao, Qianyi ; Wu, Yang ; Zhuo, Yue ; Li, Houhua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2839-923f26da022291903effd26277ce4af6b866c92ff3d72fbf763b9a31b2397a2f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Anthocyanins</topic><topic>Anthocyanins - metabolism</topic><topic>Biosynthesis</topic><topic>Chaenomeles speciosa</topic><topic>Color</topic><topic>Cuttings</topic><topic>Drought</topic><topic>Droughts</topic><topic>Flowers - genetics</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Metabolites</topic><topic>Ornamental plants</topic><topic>Petals</topic><topic>Plant Proteins - metabolism</topic><topic>Rosaceae - genetics</topic><topic>Rosaceae - metabolism</topic><topic>Transcription factors</topic><topic>Transcription Factors - metabolism</topic><topic>Transcriptomics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Shuangyu</creatorcontrib><creatorcontrib>Ren, Yanshen</creatorcontrib><creatorcontrib>Zhao, Qianyi</creatorcontrib><creatorcontrib>Wu, Yang</creatorcontrib><creatorcontrib>Zhuo, Yue</creatorcontrib><creatorcontrib>Li, Houhua</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Physiologia plantarum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Shuangyu</au><au>Ren, Yanshen</au><au>Zhao, Qianyi</au><au>Wu, Yang</au><au>Zhuo, Yue</au><au>Li, Houhua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Drought‐induced CsMYB6 interacts with CsbHLH111 to regulate anthocyanin biosynthesis in Chaenomeles speciosa</atitle><jtitle>Physiologia plantarum</jtitle><addtitle>Physiol Plant</addtitle><date>2023-01</date><risdate>2023</risdate><volume>175</volume><issue>1</issue><spage>e13859</spage><epage>n/a</epage><pages>e13859-n/a</pages><issn>0031-9317</issn><eissn>1399-3054</eissn><abstract>Chaenomeles speciosa is a plant with high ornamental value, and the color of its petals deepens obviously under drought stress. To understand the mechanism of drought‐induced reddening of C. speciosa petal color, the metabolites and transcriptomics of petals from 4% PEG‐8000‐treated and control cuttings were analyzed. In this study, the analysis of metabolites revealed the accumulation of anthocyanins in petals of PEG‐treated cuttings, indicating anthocyanins might be the reason for the deepening of petal color. By using transcriptomics, we identified CsMYB6 as an overexpressed transcription factor in PEG‐treated samples. Transient overexpression and suppression of CsMYB6 revealed that it is a key transcription factor for anthocyanin synthesis. We identified genes related to anthocyanin biosynthesis and constructed a network of drought‐ and anthocyanin‐related genes (such as CsMYB6, CsbHLH111, CsANS, CsDFR, and CsUFGT). Further experiments indicated that CsMYB6 directly interacted with CsbHLH111, and this interaction increased the binding ability of CsMYB6 to the promoter regions of three structural genes of anthocyanin biosynthesis: CsANS, CsDFR, and CsUFGT. Our findings provide a molecular basis and new insight into drought‐induced anthocyanin biosynthesis in C. speciosa.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>36688571</pmid><doi>10.1111/ppl.13859</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-5247-5508</orcidid></addata></record> |
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| subjects | Anthocyanins Anthocyanins - metabolism Biosynthesis Chaenomeles speciosa Color Cuttings Drought Droughts Flowers - genetics Gene Expression Profiling Gene Expression Regulation, Plant Genes Metabolites Ornamental plants Petals Plant Proteins - metabolism Rosaceae - genetics Rosaceae - metabolism Transcription factors Transcription Factors - metabolism Transcriptomics |
| title | Drought‐induced CsMYB6 interacts with CsbHLH111 to regulate anthocyanin biosynthesis in Chaenomeles speciosa |
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