Preparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/chitosan-gelatin network composite scaffolds
A novel biodegradable hydroxyapatite/chitosan-gelatin network (HA/CS-Gel) composite of similar composition to that of normal human bone was prepared as a three-dimensional biomimetic scaffold by phase separation method for bone tissue engineering. Changing the solid content and the compositional var...
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| Veröffentlicht in: | Biomaterials 2002-08, Vol.23 (15), p.3227-3234 |
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| creator | Zhao, Feng Yin, Yuji Lu, William W. Leong, J.Chiyan Zhang, Wenyi Zhang, Jingyu Zhang, Mingfang Yao, Kangde |
| description | A novel biodegradable hydroxyapatite/chitosan-gelatin network (HA/CS-Gel) composite of similar composition to that of normal human bone was prepared as a three-dimensional biomimetic scaffold by phase separation method for bone tissue engineering. Changing the solid content and the compositional variables of the original mixtures allowed control of the porosities and densities of the scaffolds. The HA granules were dispersed uniformly in the organic network with intimate interface contact via pulverizing and ultrasonically treating commercial available HA particles. Scaffolds of 90.6% porosity were used to examine the proliferation and functions of the cells in this three-dimensional microenvironment by culturing neonatal rat caldaria osteoblasts. Histological and immunohistochemical staining and scanning electron microscopy observation indicated that the osteoblasts attached to and proliferated on the scaffolds. Extracellular matrices including collagen I and proteoglycan-like substrate were synthesized, while osteoid and bone-like tissue formed during the culture period. Furthermore, the cell/scaffold constructs had good biomineralization effect after 3 weeks in culture. |
| doi_str_mv | 10.1016/S0142-9612(02)00077-7 |
| format | Article |
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Changing the solid content and the compositional variables of the original mixtures allowed control of the porosities and densities of the scaffolds. The HA granules were dispersed uniformly in the organic network with intimate interface contact via pulverizing and ultrasonically treating commercial available HA particles. Scaffolds of 90.6% porosity were used to examine the proliferation and functions of the cells in this three-dimensional microenvironment by culturing neonatal rat caldaria osteoblasts. Histological and immunohistochemical staining and scanning electron microscopy observation indicated that the osteoblasts attached to and proliferated on the scaffolds. Extracellular matrices including collagen I and proteoglycan-like substrate were synthesized, while osteoid and bone-like tissue formed during the culture period. Furthermore, the cell/scaffold constructs had good biomineralization effect after 3 weeks in culture.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/S0142-9612(02)00077-7</identifier><identifier>PMID: 12102194</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Animals ; Biocompatible Materials - isolation & purification ; Biodegradation, Environmental ; Biomimetic ; Bone tissue engineering ; Cells, Cultured ; Chitin - analogs & derivatives ; Chitin - isolation & purification ; Chitosan ; Durapatite - isolation & purification ; Gelatin ; Gelatin - isolation & purification ; Humans ; Hydroxyapatite ; Materials Testing ; Microscopy, Electron, Scanning ; Osteoblasts ; Osteoblasts - cytology ; Osteoblasts - metabolism ; Rats ; Scaffolds ; Tissue Engineering</subject><ispartof>Biomaterials, 2002-08, Vol.23 (15), p.3227-3234</ispartof><rights>2002 Elsevier Science Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c541t-c74d67ffa69f445dcd61a82b0cd5a6cc4e2768a41445a12e4137567c9df730663</citedby><cites>FETCH-LOGICAL-c541t-c74d67ffa69f445dcd61a82b0cd5a6cc4e2768a41445a12e4137567c9df730663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0142-9612(02)00077-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12102194$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Feng</creatorcontrib><creatorcontrib>Yin, Yuji</creatorcontrib><creatorcontrib>Lu, William W.</creatorcontrib><creatorcontrib>Leong, J.Chiyan</creatorcontrib><creatorcontrib>Zhang, Wenyi</creatorcontrib><creatorcontrib>Zhang, Jingyu</creatorcontrib><creatorcontrib>Zhang, Mingfang</creatorcontrib><creatorcontrib>Yao, Kangde</creatorcontrib><title>Preparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/chitosan-gelatin network composite scaffolds</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>A novel biodegradable hydroxyapatite/chitosan-gelatin network (HA/CS-Gel) composite of similar composition to that of normal human bone was prepared as a three-dimensional biomimetic scaffold by phase separation method for bone tissue engineering. Changing the solid content and the compositional variables of the original mixtures allowed control of the porosities and densities of the scaffolds. The HA granules were dispersed uniformly in the organic network with intimate interface contact via pulverizing and ultrasonically treating commercial available HA particles. Scaffolds of 90.6% porosity were used to examine the proliferation and functions of the cells in this three-dimensional microenvironment by culturing neonatal rat caldaria osteoblasts. Histological and immunohistochemical staining and scanning electron microscopy observation indicated that the osteoblasts attached to and proliferated on the scaffolds. Extracellular matrices including collagen I and proteoglycan-like substrate were synthesized, while osteoid and bone-like tissue formed during the culture period. Furthermore, the cell/scaffold constructs had good biomineralization effect after 3 weeks in culture.</description><subject>Animals</subject><subject>Biocompatible Materials - isolation & purification</subject><subject>Biodegradation, Environmental</subject><subject>Biomimetic</subject><subject>Bone tissue engineering</subject><subject>Cells, Cultured</subject><subject>Chitin - analogs & derivatives</subject><subject>Chitin - isolation & purification</subject><subject>Chitosan</subject><subject>Durapatite - isolation & purification</subject><subject>Gelatin</subject><subject>Gelatin - isolation & purification</subject><subject>Humans</subject><subject>Hydroxyapatite</subject><subject>Materials Testing</subject><subject>Microscopy, Electron, Scanning</subject><subject>Osteoblasts</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - metabolism</subject><subject>Rats</subject><subject>Scaffolds</subject><subject>Tissue Engineering</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFvEzEQhS0EomngJ1DtCdHDNrbjtXdPVVXRFqkSSMDZcsbjxnR3vbWdQs78cZwmokeQRhqN3_dmJD9C3jF6xiiTi6-UCV53kvEPlJ9SSpWq1QsyY61q66ajzUsy-4sckeOUftAyU8FfkyPGGeWsEzPy-0vEyUSTfRgrM9pq7VMOfbjzYPoKH02_2WvBVSsfBj9g9lDldUSsbZnGVNSCrrc2hl9bMxU84wLWPodkxvoO-_IyViPmnyHeVxCGKaSCVAmMc6G36Q155Uyf8O2hz8n3q4_fLm_q28_Xny4vbmtoBMs1KGGlcs7IzgnRWLCSmZavKNjGSACBXMnWCFZEwzgKtlSNVNBZp5ZUyuWcvN_vnWJ42GDKevAJsO_NiGGTdLEXsKX_CS6bAjZ7EGJIKaLTU_SDiVvNqN7FpJ9i0rsMNC21i0mr4js5HNisBrTPrkMuBTjfA1j-49Fj1Ak8joDWR4SsbfD_OPEH7JWlrw</recordid><startdate>20020801</startdate><enddate>20020801</enddate><creator>Zhao, Feng</creator><creator>Yin, Yuji</creator><creator>Lu, William W.</creator><creator>Leong, J.Chiyan</creator><creator>Zhang, Wenyi</creator><creator>Zhang, Jingyu</creator><creator>Zhang, Mingfang</creator><creator>Yao, Kangde</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QQ</scope><scope>8FD</scope><scope>JG9</scope><scope>F28</scope><scope>FR3</scope></search><sort><creationdate>20020801</creationdate><title>Preparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/chitosan-gelatin network composite scaffolds</title><author>Zhao, Feng ; Yin, Yuji ; Lu, William W. ; Leong, J.Chiyan ; Zhang, Wenyi ; Zhang, Jingyu ; Zhang, Mingfang ; Yao, Kangde</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c541t-c74d67ffa69f445dcd61a82b0cd5a6cc4e2768a41445a12e4137567c9df730663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Biocompatible Materials - isolation & purification</topic><topic>Biodegradation, Environmental</topic><topic>Biomimetic</topic><topic>Bone tissue engineering</topic><topic>Cells, Cultured</topic><topic>Chitin - analogs & derivatives</topic><topic>Chitin - isolation & purification</topic><topic>Chitosan</topic><topic>Durapatite - isolation & purification</topic><topic>Gelatin</topic><topic>Gelatin - isolation & purification</topic><topic>Humans</topic><topic>Hydroxyapatite</topic><topic>Materials Testing</topic><topic>Microscopy, Electron, Scanning</topic><topic>Osteoblasts</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - metabolism</topic><topic>Rats</topic><topic>Scaffolds</topic><topic>Tissue Engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Feng</creatorcontrib><creatorcontrib>Yin, Yuji</creatorcontrib><creatorcontrib>Lu, William W.</creatorcontrib><creatorcontrib>Leong, J.Chiyan</creatorcontrib><creatorcontrib>Zhang, Wenyi</creatorcontrib><creatorcontrib>Zhang, Jingyu</creatorcontrib><creatorcontrib>Zhang, Mingfang</creatorcontrib><creatorcontrib>Yao, Kangde</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Ceramic Abstracts</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Feng</au><au>Yin, Yuji</au><au>Lu, William W.</au><au>Leong, J.Chiyan</au><au>Zhang, Wenyi</au><au>Zhang, Jingyu</au><au>Zhang, Mingfang</au><au>Yao, Kangde</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/chitosan-gelatin network composite scaffolds</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2002-08-01</date><risdate>2002</risdate><volume>23</volume><issue>15</issue><spage>3227</spage><epage>3234</epage><pages>3227-3234</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>A novel biodegradable hydroxyapatite/chitosan-gelatin network (HA/CS-Gel) composite of similar composition to that of normal human bone was prepared as a three-dimensional biomimetic scaffold by phase separation method for bone tissue engineering. Changing the solid content and the compositional variables of the original mixtures allowed control of the porosities and densities of the scaffolds. The HA granules were dispersed uniformly in the organic network with intimate interface contact via pulverizing and ultrasonically treating commercial available HA particles. Scaffolds of 90.6% porosity were used to examine the proliferation and functions of the cells in this three-dimensional microenvironment by culturing neonatal rat caldaria osteoblasts. Histological and immunohistochemical staining and scanning electron microscopy observation indicated that the osteoblasts attached to and proliferated on the scaffolds. Extracellular matrices including collagen I and proteoglycan-like substrate were synthesized, while osteoid and bone-like tissue formed during the culture period. Furthermore, the cell/scaffold constructs had good biomineralization effect after 3 weeks in culture.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>12102194</pmid><doi>10.1016/S0142-9612(02)00077-7</doi><tpages>8</tpages></addata></record> |
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| issn | 0142-9612 1878-5905 |
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| subjects | Animals Biocompatible Materials - isolation & purification Biodegradation, Environmental Biomimetic Bone tissue engineering Cells, Cultured Chitin - analogs & derivatives Chitin - isolation & purification Chitosan Durapatite - isolation & purification Gelatin Gelatin - isolation & purification Humans Hydroxyapatite Materials Testing Microscopy, Electron, Scanning Osteoblasts Osteoblasts - cytology Osteoblasts - metabolism Rats Scaffolds Tissue Engineering |
| title | Preparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/chitosan-gelatin network composite scaffolds |
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