A versatile biosensing platform coupling CRISPR–Cas12a and aptamers for detection of diverse analytes
[Display omitted] Rapid and sensitive detection of various analytes is in high demand. Apart from its application in genome editing, CRISPR–Cas also shows promises in nucleic acid detection applications. To further exploit the potential of CRISPR–Cas for detection of diverse analytes, we present a v...
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| Veröffentlicht in: | Science bulletin 2021-01, Vol.66 (1), p.69-77 |
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| creator | Zhao, Xiangxiang Li, Shanshan Liu, Guang Wang, Zhong Yang, Zhiheng Zhang, Quanwei Liang, Mindong Liu, Jiakun Li, Zilong Tong, Yaojun Zhu, Guoliang Wang, Xinye Jiang, Lan Wang, Weishan Tan, Gao-Yi Zhang, Lixin |
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Rapid and sensitive detection of various analytes is in high demand. Apart from its application in genome editing, CRISPR–Cas also shows promises in nucleic acid detection applications. To further exploit the potential of CRISPR–Cas for detection of diverse analytes, we present a versatile biosensing platform that couples the excellent affinity of aptamers for broad-range analytes with the collateral single-strand DNA cleavage activity of CRISPR–Cas12a. We demonstrated that the biosensors developed by this platform can be used to detect protein and small molecule in human serum with a complicated background, i.e., the tumor marker alpha fetoprotein and cocaine with the detection limits of 0.07 fmol/L and 0.34 μmol/L, respectively, highlighting the advantages of simplicity, sensitivity, short detection time, and low cost compared with the state-of-the-art biosensing approaches. Altogether, this biosensing platform with plug-and-play design show great potential in the detection of diverse analytes. |
| doi_str_mv | 10.1016/j.scib.2020.09.004 |
| format | Article |
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Rapid and sensitive detection of various analytes is in high demand. Apart from its application in genome editing, CRISPR–Cas also shows promises in nucleic acid detection applications. To further exploit the potential of CRISPR–Cas for detection of diverse analytes, we present a versatile biosensing platform that couples the excellent affinity of aptamers for broad-range analytes with the collateral single-strand DNA cleavage activity of CRISPR–Cas12a. We demonstrated that the biosensors developed by this platform can be used to detect protein and small molecule in human serum with a complicated background, i.e., the tumor marker alpha fetoprotein and cocaine with the detection limits of 0.07 fmol/L and 0.34 μmol/L, respectively, highlighting the advantages of simplicity, sensitivity, short detection time, and low cost compared with the state-of-the-art biosensing approaches. Altogether, this biosensing platform with plug-and-play design show great potential in the detection of diverse analytes.</description><identifier>ISSN: 2095-9273</identifier><identifier>EISSN: 2095-9281</identifier><identifier>DOI: 10.1016/j.scib.2020.09.004</identifier><identifier>PMID: 36654316</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Alpha fetoprotein detection ; Aptamer ; Biosensing platform ; Cocaine detection ; CRISPR–Cas12a ; Diverse analyte</subject><ispartof>Science bulletin, 2021-01, Vol.66 (1), p.69-77</ispartof><rights>2020 Science China Press</rights><rights>Copyright © 2020 Science China Press. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-e438f506e43e21e8bd7cf35bf0e4ebce02f2823e53e5cbfc1ffa8d4231eb876b3</citedby><cites>FETCH-LOGICAL-c356t-e438f506e43e21e8bd7cf35bf0e4ebce02f2823e53e5cbfc1ffa8d4231eb876b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36654316$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Xiangxiang</creatorcontrib><creatorcontrib>Li, Shanshan</creatorcontrib><creatorcontrib>Liu, Guang</creatorcontrib><creatorcontrib>Wang, Zhong</creatorcontrib><creatorcontrib>Yang, Zhiheng</creatorcontrib><creatorcontrib>Zhang, Quanwei</creatorcontrib><creatorcontrib>Liang, Mindong</creatorcontrib><creatorcontrib>Liu, Jiakun</creatorcontrib><creatorcontrib>Li, Zilong</creatorcontrib><creatorcontrib>Tong, Yaojun</creatorcontrib><creatorcontrib>Zhu, Guoliang</creatorcontrib><creatorcontrib>Wang, Xinye</creatorcontrib><creatorcontrib>Jiang, Lan</creatorcontrib><creatorcontrib>Wang, Weishan</creatorcontrib><creatorcontrib>Tan, Gao-Yi</creatorcontrib><creatorcontrib>Zhang, Lixin</creatorcontrib><title>A versatile biosensing platform coupling CRISPR–Cas12a and aptamers for detection of diverse analytes</title><title>Science bulletin</title><addtitle>Sci Bull (Beijing)</addtitle><description>[Display omitted]
Rapid and sensitive detection of various analytes is in high demand. Apart from its application in genome editing, CRISPR–Cas also shows promises in nucleic acid detection applications. To further exploit the potential of CRISPR–Cas for detection of diverse analytes, we present a versatile biosensing platform that couples the excellent affinity of aptamers for broad-range analytes with the collateral single-strand DNA cleavage activity of CRISPR–Cas12a. We demonstrated that the biosensors developed by this platform can be used to detect protein and small molecule in human serum with a complicated background, i.e., the tumor marker alpha fetoprotein and cocaine with the detection limits of 0.07 fmol/L and 0.34 μmol/L, respectively, highlighting the advantages of simplicity, sensitivity, short detection time, and low cost compared with the state-of-the-art biosensing approaches. Altogether, this biosensing platform with plug-and-play design show great potential in the detection of diverse analytes.</description><subject>Alpha fetoprotein detection</subject><subject>Aptamer</subject><subject>Biosensing platform</subject><subject>Cocaine detection</subject><subject>CRISPR–Cas12a</subject><subject>Diverse analyte</subject><issn>2095-9273</issn><issn>2095-9281</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kM1qGzEURkVJaYybF-iiaJmNp1fS_EI3wSRpwNCStmshaa6CzMxoIo0N3uUd-oZ9kmiw42Xgwr2I832gQ8gXBhkDVn7bZtE4nXHgkEGTAeQfyIJDU6waXrOL812JS3IV4xYAWN7wHKpP5FKUZZELVi7I0w3dY4hqch1S7XzEIbrhiY6dmqwPPTV-N3bzy_rx4fevx_8v_9YqMq6oGlqqxkn1KU4TSluc0EzOD9Rb2rq5FhOlusOE8TP5aFUX8eq0l-Tv3e2f9Y_V5uf9w_pmszKiKKcV5qK2BZRpI2dY67YyVhTaAuaoDQK3vOYCizRGW8OsVXWbc8FQ11WpxZJcH3vH4J93GCfZu2iw69SAfhclr8qKVVCwJqH8iJrgYwxo5Rhcr8JBMpCzY7mVs2M5O5bQyOQ4hb6e-ne6x_YceTOagO9HANMv9w7D3IGDwdaFpEe23r3X_wqHq49X</recordid><startdate>20210115</startdate><enddate>20210115</enddate><creator>Zhao, Xiangxiang</creator><creator>Li, Shanshan</creator><creator>Liu, Guang</creator><creator>Wang, Zhong</creator><creator>Yang, Zhiheng</creator><creator>Zhang, Quanwei</creator><creator>Liang, Mindong</creator><creator>Liu, Jiakun</creator><creator>Li, Zilong</creator><creator>Tong, Yaojun</creator><creator>Zhu, Guoliang</creator><creator>Wang, Xinye</creator><creator>Jiang, Lan</creator><creator>Wang, Weishan</creator><creator>Tan, Gao-Yi</creator><creator>Zhang, Lixin</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20210115</creationdate><title>A versatile biosensing platform coupling CRISPR–Cas12a and aptamers for detection of diverse analytes</title><author>Zhao, Xiangxiang ; Li, Shanshan ; Liu, Guang ; Wang, Zhong ; Yang, Zhiheng ; Zhang, Quanwei ; Liang, Mindong ; Liu, Jiakun ; Li, Zilong ; Tong, Yaojun ; Zhu, Guoliang ; Wang, Xinye ; Jiang, Lan ; Wang, Weishan ; Tan, Gao-Yi ; Zhang, Lixin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-e438f506e43e21e8bd7cf35bf0e4ebce02f2823e53e5cbfc1ffa8d4231eb876b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Alpha fetoprotein detection</topic><topic>Aptamer</topic><topic>Biosensing platform</topic><topic>Cocaine detection</topic><topic>CRISPR–Cas12a</topic><topic>Diverse analyte</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Xiangxiang</creatorcontrib><creatorcontrib>Li, Shanshan</creatorcontrib><creatorcontrib>Liu, Guang</creatorcontrib><creatorcontrib>Wang, Zhong</creatorcontrib><creatorcontrib>Yang, Zhiheng</creatorcontrib><creatorcontrib>Zhang, Quanwei</creatorcontrib><creatorcontrib>Liang, Mindong</creatorcontrib><creatorcontrib>Liu, Jiakun</creatorcontrib><creatorcontrib>Li, Zilong</creatorcontrib><creatorcontrib>Tong, Yaojun</creatorcontrib><creatorcontrib>Zhu, Guoliang</creatorcontrib><creatorcontrib>Wang, Xinye</creatorcontrib><creatorcontrib>Jiang, Lan</creatorcontrib><creatorcontrib>Wang, Weishan</creatorcontrib><creatorcontrib>Tan, Gao-Yi</creatorcontrib><creatorcontrib>Zhang, Lixin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Science bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Xiangxiang</au><au>Li, Shanshan</au><au>Liu, Guang</au><au>Wang, Zhong</au><au>Yang, Zhiheng</au><au>Zhang, Quanwei</au><au>Liang, Mindong</au><au>Liu, Jiakun</au><au>Li, Zilong</au><au>Tong, Yaojun</au><au>Zhu, Guoliang</au><au>Wang, Xinye</au><au>Jiang, Lan</au><au>Wang, Weishan</au><au>Tan, Gao-Yi</au><au>Zhang, Lixin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A versatile biosensing platform coupling CRISPR–Cas12a and aptamers for detection of diverse analytes</atitle><jtitle>Science bulletin</jtitle><addtitle>Sci Bull (Beijing)</addtitle><date>2021-01-15</date><risdate>2021</risdate><volume>66</volume><issue>1</issue><spage>69</spage><epage>77</epage><pages>69-77</pages><issn>2095-9273</issn><eissn>2095-9281</eissn><abstract>[Display omitted]
Rapid and sensitive detection of various analytes is in high demand. Apart from its application in genome editing, CRISPR–Cas also shows promises in nucleic acid detection applications. To further exploit the potential of CRISPR–Cas for detection of diverse analytes, we present a versatile biosensing platform that couples the excellent affinity of aptamers for broad-range analytes with the collateral single-strand DNA cleavage activity of CRISPR–Cas12a. We demonstrated that the biosensors developed by this platform can be used to detect protein and small molecule in human serum with a complicated background, i.e., the tumor marker alpha fetoprotein and cocaine with the detection limits of 0.07 fmol/L and 0.34 μmol/L, respectively, highlighting the advantages of simplicity, sensitivity, short detection time, and low cost compared with the state-of-the-art biosensing approaches. Altogether, this biosensing platform with plug-and-play design show great potential in the detection of diverse analytes.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>36654316</pmid><doi>10.1016/j.scib.2020.09.004</doi><tpages>9</tpages></addata></record> |
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| source | Alma/SFX Local Collection |
| subjects | Alpha fetoprotein detection Aptamer Biosensing platform Cocaine detection CRISPR–Cas12a Diverse analyte |
| title | A versatile biosensing platform coupling CRISPR–Cas12a and aptamers for detection of diverse analytes |
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