Molecular phenotypes of bovine blastocyst derived from in vitro-matured oocyte supplemented with PAPP-A
Insulin-like growth factor-1 (IGF-1) regulates cellular lipid content, whereas pregnancy-associated plasma protein-A (PAPP-A) increases IGF-1 bioavailability. Using in vitro -matured cumulus-oocyte complexes, we aimed to evaluate the impact of PAPP-A on the blastocyst lipid content, embryo cryotoler...
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| Veröffentlicht in: | Veterinary research communications 2023-09, Vol.47 (3), p.1263-1272 |
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| creator | Dellaqua, Thaisy Tino Franchi, Fernanda Fagali dos Santos, Priscila Helena Giroto, Alan Brunholi Nunes, Sarah Gomes de Lima, Victor Augusto Vieira Guilherme, Vitória Bertogna Fontes, Patrícia Kubo Sudano, Mateus José de Souza Castilho, Anthony César |
| description | Insulin-like growth factor-1 (IGF-1) regulates cellular lipid content, whereas pregnancy-associated plasma protein-A (PAPP-A) increases IGF-1 bioavailability. Using
in vitro
-matured cumulus-oocyte complexes, we aimed to evaluate the impact of PAPP-A on the blastocyst lipid content, embryo cryotolerance and embryonic transcriptional profile. We determined that PAPP-A did not affect the lipid content of oocytes, blastocysts, or blastocyst yield (
P
> 0.05). However, PAPP-A modulated the embryo transcriptional profiles by downregulating
PPARGC1A
and
AKR1B1
, which are related to lipid metabolism;
CASP9
, a pro-apoptotic gene; and
IFN-τ
, a marker of embryo quality (
P
|
| doi_str_mv | 10.1007/s11259-023-10072-7 |
| format | Article |
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in vitro
-matured cumulus-oocyte complexes, we aimed to evaluate the impact of PAPP-A on the blastocyst lipid content, embryo cryotolerance and embryonic transcriptional profile. We determined that PAPP-A did not affect the lipid content of oocytes, blastocysts, or blastocyst yield (
P
> 0.05). However, PAPP-A modulated the embryo transcriptional profiles by downregulating
PPARGC1A
and
AKR1B1
, which are related to lipid metabolism;
CASP9
, a pro-apoptotic gene; and
IFN-τ
, a marker of embryo quality (
P
< 0.05). Furthermore, the use of PAPP-A improved blastocyst re-expansion in the first 3 h of culture after vitrification (
P
< 0.05). Although PAPP-A did not affect the blastocyst lipid content or embryo production, we suggest that embryonic transcriptional modulation could contribute to maintain the balance in embryo lipid metabolism. Furthermore, PAPP-A’s approach seems to control key intracellular pathways that improve post-cryopreservation development of blastocysts.</description><identifier>ISSN: 0165-7380</identifier><identifier>EISSN: 1573-7446</identifier><identifier>DOI: 10.1007/s11259-023-10072-7</identifier><identifier>PMID: 36653723</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Apoptosis ; Bioavailability ; Biology ; Biomedical and Life Sciences ; Blastocysts ; Cattle ; Cell culture ; Cold tolerance ; Cryopreservation ; Embryos ; Energy ; Fatty acids ; Gene expression ; In vitro fertilization ; Insulin ; Insulin-like growth factor I ; Insulin-like growth factors ; Life Sciences ; Lipid metabolism ; Lipids ; Metabolism ; Oocytes ; Phenotypes ; Pregnancy ; Proteins ; Sperm ; Transcription ; Triglycerides ; Veterinary medicine ; Veterinary Medicine/Veterinary Science ; Vitrification ; Zoology</subject><ispartof>Veterinary research communications, 2023-09, Vol.47 (3), p.1263-1272</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-7fb57301d659e470f1ed8e26fd0252c53774dd2ad80cd19b38dc98b86a5f2da13</cites><orcidid>0000-0002-7879-6534 ; 0000-0002-9859-0576 ; 0000-0003-1666-7021</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11259-023-10072-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11259-023-10072-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36653723$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dellaqua, Thaisy Tino</creatorcontrib><creatorcontrib>Franchi, Fernanda Fagali</creatorcontrib><creatorcontrib>dos Santos, Priscila Helena</creatorcontrib><creatorcontrib>Giroto, Alan Brunholi</creatorcontrib><creatorcontrib>Nunes, Sarah Gomes</creatorcontrib><creatorcontrib>de Lima, Victor Augusto Vieira</creatorcontrib><creatorcontrib>Guilherme, Vitória Bertogna</creatorcontrib><creatorcontrib>Fontes, Patrícia Kubo</creatorcontrib><creatorcontrib>Sudano, Mateus José</creatorcontrib><creatorcontrib>de Souza Castilho, Anthony César</creatorcontrib><title>Molecular phenotypes of bovine blastocyst derived from in vitro-matured oocyte supplemented with PAPP-A</title><title>Veterinary research communications</title><addtitle>Vet Res Commun</addtitle><addtitle>Vet Res Commun</addtitle><description>Insulin-like growth factor-1 (IGF-1) regulates cellular lipid content, whereas pregnancy-associated plasma protein-A (PAPP-A) increases IGF-1 bioavailability. Using
in vitro
-matured cumulus-oocyte complexes, we aimed to evaluate the impact of PAPP-A on the blastocyst lipid content, embryo cryotolerance and embryonic transcriptional profile. We determined that PAPP-A did not affect the lipid content of oocytes, blastocysts, or blastocyst yield (
P
> 0.05). However, PAPP-A modulated the embryo transcriptional profiles by downregulating
PPARGC1A
and
AKR1B1
, which are related to lipid metabolism;
CASP9
, a pro-apoptotic gene; and
IFN-τ
, a marker of embryo quality (
P
< 0.05). Furthermore, the use of PAPP-A improved blastocyst re-expansion in the first 3 h of culture after vitrification (
P
< 0.05). Although PAPP-A did not affect the blastocyst lipid content or embryo production, we suggest that embryonic transcriptional modulation could contribute to maintain the balance in embryo lipid metabolism. Furthermore, PAPP-A’s approach seems to control key intracellular pathways that improve post-cryopreservation development of blastocysts.</description><subject>Apoptosis</subject><subject>Bioavailability</subject><subject>Biology</subject><subject>Biomedical and Life Sciences</subject><subject>Blastocysts</subject><subject>Cattle</subject><subject>Cell culture</subject><subject>Cold tolerance</subject><subject>Cryopreservation</subject><subject>Embryos</subject><subject>Energy</subject><subject>Fatty acids</subject><subject>Gene expression</subject><subject>In vitro fertilization</subject><subject>Insulin</subject><subject>Insulin-like growth factor I</subject><subject>Insulin-like growth factors</subject><subject>Life Sciences</subject><subject>Lipid metabolism</subject><subject>Lipids</subject><subject>Metabolism</subject><subject>Oocytes</subject><subject>Phenotypes</subject><subject>Pregnancy</subject><subject>Proteins</subject><subject>Sperm</subject><subject>Transcription</subject><subject>Triglycerides</subject><subject>Veterinary medicine</subject><subject>Veterinary Medicine/Veterinary 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fertilization</topic><topic>Insulin</topic><topic>Insulin-like growth factor I</topic><topic>Insulin-like growth factors</topic><topic>Life Sciences</topic><topic>Lipid metabolism</topic><topic>Lipids</topic><topic>Metabolism</topic><topic>Oocytes</topic><topic>Phenotypes</topic><topic>Pregnancy</topic><topic>Proteins</topic><topic>Sperm</topic><topic>Transcription</topic><topic>Triglycerides</topic><topic>Veterinary medicine</topic><topic>Veterinary Medicine/Veterinary Science</topic><topic>Vitrification</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dellaqua, Thaisy Tino</creatorcontrib><creatorcontrib>Franchi, Fernanda Fagali</creatorcontrib><creatorcontrib>dos Santos, Priscila Helena</creatorcontrib><creatorcontrib>Giroto, Alan Brunholi</creatorcontrib><creatorcontrib>Nunes, Sarah Gomes</creatorcontrib><creatorcontrib>de Lima, Victor Augusto Vieira</creatorcontrib><creatorcontrib>Guilherme, Vitória 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Commun</stitle><addtitle>Vet Res Commun</addtitle><date>2023-09-01</date><risdate>2023</risdate><volume>47</volume><issue>3</issue><spage>1263</spage><epage>1272</epage><pages>1263-1272</pages><issn>0165-7380</issn><eissn>1573-7446</eissn><abstract>Insulin-like growth factor-1 (IGF-1) regulates cellular lipid content, whereas pregnancy-associated plasma protein-A (PAPP-A) increases IGF-1 bioavailability. Using
in vitro
-matured cumulus-oocyte complexes, we aimed to evaluate the impact of PAPP-A on the blastocyst lipid content, embryo cryotolerance and embryonic transcriptional profile. We determined that PAPP-A did not affect the lipid content of oocytes, blastocysts, or blastocyst yield (
P
> 0.05). However, PAPP-A modulated the embryo transcriptional profiles by downregulating
PPARGC1A
and
AKR1B1
, which are related to lipid metabolism;
CASP9
, a pro-apoptotic gene; and
IFN-τ
, a marker of embryo quality (
P
< 0.05). Furthermore, the use of PAPP-A improved blastocyst re-expansion in the first 3 h of culture after vitrification (
P
< 0.05). Although PAPP-A did not affect the blastocyst lipid content or embryo production, we suggest that embryonic transcriptional modulation could contribute to maintain the balance in embryo lipid metabolism. Furthermore, PAPP-A’s approach seems to control key intracellular pathways that improve post-cryopreservation development of blastocysts.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>36653723</pmid><doi>10.1007/s11259-023-10072-7</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-7879-6534</orcidid><orcidid>https://orcid.org/0000-0002-9859-0576</orcidid><orcidid>https://orcid.org/0000-0003-1666-7021</orcidid></addata></record> |
| fulltext | fulltext |
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| ispartof | Veterinary research communications, 2023-09, Vol.47 (3), p.1263-1272 |
| issn | 0165-7380 1573-7446 |
| language | eng |
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| source | SpringerLink Journals |
| subjects | Apoptosis Bioavailability Biology Biomedical and Life Sciences Blastocysts Cattle Cell culture Cold tolerance Cryopreservation Embryos Energy Fatty acids Gene expression In vitro fertilization Insulin Insulin-like growth factor I Insulin-like growth factors Life Sciences Lipid metabolism Lipids Metabolism Oocytes Phenotypes Pregnancy Proteins Sperm Transcription Triglycerides Veterinary medicine Veterinary Medicine/Veterinary Science Vitrification Zoology |
| title | Molecular phenotypes of bovine blastocyst derived from in vitro-matured oocyte supplemented with PAPP-A |
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