Establishment of Korean Pediatric Reference Intervals for Estradiol using Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry
•First established reference intervals for estradiol using a UHPLC-MS/MS-based method in South Korea.•Serum samples from 634 pediatric patients, including neonates, infants, children, and adolescents.•The highly analytical sensitive UHPLC-MS/MS method was useful for estradiol determination in childr...
Gespeichert in:
| Veröffentlicht in: | Clinical biochemistry 2023-03, Vol.113, p.52-58 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 58 |
|---|---|
| container_issue | |
| container_start_page | 52 |
| container_title | Clinical biochemistry |
| container_volume | 113 |
| creator | Won, Eun Jeong Yi, Ahram Ko, Young Jin Kim, Serim Kang, Seong-Ho Park, Geon Jang, Sook-Jin |
| description | •First established reference intervals for estradiol using a UHPLC-MS/MS-based method in South Korea.•Serum samples from 634 pediatric patients, including neonates, infants, children, and adolescents.•The highly analytical sensitive UHPLC-MS/MS method was useful for estradiol determination in children.
Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS) is a reliable and accurate method for measuring steroid hormone levels. There is an increasing need for sensitive and precise methods to measure estradiol in pediatric patients. Here, we established reference intervals for estradiol in healthy children using a UHPLC–MS/MS-based method for the first time in South Korea.
Serum estradiol was measured using a Sciex Triple QuadTM 6500 + UHPLC–MS/MS (Sciex, Framingham, MA, USA). Reference intervals for estradiol were established according to the CLSI document EP28-A3c:2008. The reference intervals were validated using serum samples from 634 pediatric patients, including neonates, children, and adolescents. Among them, 389 specimens were used in analysis of the specimen acceptance time. Statistical analysis was performed using MedCalc (MedCalc, Ostend, Belgium) and Analyse–it (Analyse–it Software Ltd., Leeds, United Kingdom) software.
Reference intervals for boys (n = 297) were |
| doi_str_mv | 10.1016/j.clinbiochem.2023.01.004 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2765772033</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0009912023000048</els_id><sourcerecordid>2765772033</sourcerecordid><originalsourceid>FETCH-LOGICAL-c377t-463daae1c07beaad5236889aeb023354287f64319fb6e2284a4c4230bfc841763</originalsourceid><addsrcrecordid>eNqNkc1u1DAUhS0EokPhFZDZsUnw39jJEo1aWjGICtq15dg3E4-SeGo7leYpeGU8moJYsrKufM49uudD6AMlNSVUftrXdvRz54MdYKoZYbwmtCZEvEAr2ihesZbzl2hFCGmrljJygd6ktC8jE418jS64lEwRSlfo11XKpht9GiaYMw49_hoimBnfgfMmR2_xD-ghwmwB384Z4pMZE-5DxMUZjfNhxEvy8w4_jGWubvxuqO4gFsVkTqatf1y8w5shhsnksIvmMByrezM7mPA3kxL-eQCbyy_keHyLXvUlAN49v5fo4frqfnNTbb9_ud183laWK5UrIbkzBqglqgNj3Jpx2TStga6UwdeCNaqXgtO27yQw1ggjrGCcdL1tBFWSX6KP572HGB4XSFlPPlkYRzNDWJJmSq6VYoTzIm3PUhtDShF6fYh-MvGoKdEnHnqv_-GhTzw0obrwKN73zzFLN4H76_wDoAg2ZwGUY588RJ2sP5XtfCytaBf8f8T8BuqQpLU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2765772033</pqid></control><display><type>article</type><title>Establishment of Korean Pediatric Reference Intervals for Estradiol using Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Won, Eun Jeong ; Yi, Ahram ; Ko, Young Jin ; Kim, Serim ; Kang, Seong-Ho ; Park, Geon ; Jang, Sook-Jin</creator><creatorcontrib>Won, Eun Jeong ; Yi, Ahram ; Ko, Young Jin ; Kim, Serim ; Kang, Seong-Ho ; Park, Geon ; Jang, Sook-Jin</creatorcontrib><description><![CDATA[•First established reference intervals for estradiol using a UHPLC-MS/MS-based method in South Korea.•Serum samples from 634 pediatric patients, including neonates, infants, children, and adolescents.•The highly analytical sensitive UHPLC-MS/MS method was useful for estradiol determination in children.
Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS) is a reliable and accurate method for measuring steroid hormone levels. There is an increasing need for sensitive and precise methods to measure estradiol in pediatric patients. Here, we established reference intervals for estradiol in healthy children using a UHPLC–MS/MS-based method for the first time in South Korea.
Serum estradiol was measured using a Sciex Triple QuadTM 6500 + UHPLC–MS/MS (Sciex, Framingham, MA, USA). Reference intervals for estradiol were established according to the CLSI document EP28-A3c:2008. The reference intervals were validated using serum samples from 634 pediatric patients, including neonates, children, and adolescents. Among them, 389 specimens were used in analysis of the specimen acceptance time. Statistical analysis was performed using MedCalc (MedCalc, Ostend, Belgium) and Analyse–it (Analyse–it Software Ltd., Leeds, United Kingdom) software.
Reference intervals for boys (n = 297) were <16.6, <7.3, <19.0, <30.5, 7.6–96.5, and 10.6–134.4 pmol/L among those aged <1, 1–5, 6–9, 10–11, 12–14, and 15–17 years, respectively. Reference intervals for girls (n = 337) were <114.7, <24.2, <34.8, 8.0–177.0, 10.4–480.5, and 9.1–486.7 pmol/L among those aged <1, 1–5, 6–9, 10–11, 12–14, and 15–17 years, respectively. Overall, there was no effect of specimen acceptance time on estradiol measurements in boys or girls, except for that in the group aged 10–11 years.
The reference intervals for healthy children were validated using a UHPLC–MS/MS-based method. The highly analytical sensitive UHPLC–MS/MS method may be useful for estradiol determination in pediatric patients.]]></description><identifier>ISSN: 0009-9120</identifier><identifier>EISSN: 1873-2933</identifier><identifier>DOI: 10.1016/j.clinbiochem.2023.01.004</identifier><identifier>PMID: 36627011</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adolescent ; Child ; Chromatography, High Pressure Liquid ; Estradiol ; Female ; High-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS) ; Humans ; Infant, Newborn ; Male ; Reference intervals ; Reference Values ; Software ; Tandem Mass Spectrometry - methods</subject><ispartof>Clinical biochemistry, 2023-03, Vol.113, p.52-58</ispartof><rights>2023 The Canadian Society of Clinical Chemists</rights><rights>Copyright © 2023 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-463daae1c07beaad5236889aeb023354287f64319fb6e2284a4c4230bfc841763</citedby><cites>FETCH-LOGICAL-c377t-463daae1c07beaad5236889aeb023354287f64319fb6e2284a4c4230bfc841763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.clinbiochem.2023.01.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36627011$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Won, Eun Jeong</creatorcontrib><creatorcontrib>Yi, Ahram</creatorcontrib><creatorcontrib>Ko, Young Jin</creatorcontrib><creatorcontrib>Kim, Serim</creatorcontrib><creatorcontrib>Kang, Seong-Ho</creatorcontrib><creatorcontrib>Park, Geon</creatorcontrib><creatorcontrib>Jang, Sook-Jin</creatorcontrib><title>Establishment of Korean Pediatric Reference Intervals for Estradiol using Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry</title><title>Clinical biochemistry</title><addtitle>Clin Biochem</addtitle><description><![CDATA[•First established reference intervals for estradiol using a UHPLC-MS/MS-based method in South Korea.•Serum samples from 634 pediatric patients, including neonates, infants, children, and adolescents.•The highly analytical sensitive UHPLC-MS/MS method was useful for estradiol determination in children.
Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS) is a reliable and accurate method for measuring steroid hormone levels. There is an increasing need for sensitive and precise methods to measure estradiol in pediatric patients. Here, we established reference intervals for estradiol in healthy children using a UHPLC–MS/MS-based method for the first time in South Korea.
Serum estradiol was measured using a Sciex Triple QuadTM 6500 + UHPLC–MS/MS (Sciex, Framingham, MA, USA). Reference intervals for estradiol were established according to the CLSI document EP28-A3c:2008. The reference intervals were validated using serum samples from 634 pediatric patients, including neonates, children, and adolescents. Among them, 389 specimens were used in analysis of the specimen acceptance time. Statistical analysis was performed using MedCalc (MedCalc, Ostend, Belgium) and Analyse–it (Analyse–it Software Ltd., Leeds, United Kingdom) software.
Reference intervals for boys (n = 297) were <16.6, <7.3, <19.0, <30.5, 7.6–96.5, and 10.6–134.4 pmol/L among those aged <1, 1–5, 6–9, 10–11, 12–14, and 15–17 years, respectively. Reference intervals for girls (n = 337) were <114.7, <24.2, <34.8, 8.0–177.0, 10.4–480.5, and 9.1–486.7 pmol/L among those aged <1, 1–5, 6–9, 10–11, 12–14, and 15–17 years, respectively. Overall, there was no effect of specimen acceptance time on estradiol measurements in boys or girls, except for that in the group aged 10–11 years.
The reference intervals for healthy children were validated using a UHPLC–MS/MS-based method. The highly analytical sensitive UHPLC–MS/MS method may be useful for estradiol determination in pediatric patients.]]></description><subject>Adolescent</subject><subject>Child</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Estradiol</subject><subject>Female</subject><subject>High-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS)</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Male</subject><subject>Reference intervals</subject><subject>Reference Values</subject><subject>Software</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>0009-9120</issn><issn>1873-2933</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EokPhFZDZsUnw39jJEo1aWjGICtq15dg3E4-SeGo7leYpeGU8moJYsrKufM49uudD6AMlNSVUftrXdvRz54MdYKoZYbwmtCZEvEAr2ihesZbzl2hFCGmrljJygd6ktC8jE418jS64lEwRSlfo11XKpht9GiaYMw49_hoimBnfgfMmR2_xD-ghwmwB384Z4pMZE-5DxMUZjfNhxEvy8w4_jGWubvxuqO4gFsVkTqatf1y8w5shhsnksIvmMByrezM7mPA3kxL-eQCbyy_keHyLXvUlAN49v5fo4frqfnNTbb9_ud183laWK5UrIbkzBqglqgNj3Jpx2TStga6UwdeCNaqXgtO27yQw1ggjrGCcdL1tBFWSX6KP572HGB4XSFlPPlkYRzNDWJJmSq6VYoTzIm3PUhtDShF6fYh-MvGoKdEnHnqv_-GhTzw0obrwKN73zzFLN4H76_wDoAg2ZwGUY588RJ2sP5XtfCytaBf8f8T8BuqQpLU</recordid><startdate>202303</startdate><enddate>202303</enddate><creator>Won, Eun Jeong</creator><creator>Yi, Ahram</creator><creator>Ko, Young Jin</creator><creator>Kim, Serim</creator><creator>Kang, Seong-Ho</creator><creator>Park, Geon</creator><creator>Jang, Sook-Jin</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202303</creationdate><title>Establishment of Korean Pediatric Reference Intervals for Estradiol using Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry</title><author>Won, Eun Jeong ; Yi, Ahram ; Ko, Young Jin ; Kim, Serim ; Kang, Seong-Ho ; Park, Geon ; Jang, Sook-Jin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-463daae1c07beaad5236889aeb023354287f64319fb6e2284a4c4230bfc841763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Adolescent</topic><topic>Child</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Estradiol</topic><topic>Female</topic><topic>High-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS)</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Male</topic><topic>Reference intervals</topic><topic>Reference Values</topic><topic>Software</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Won, Eun Jeong</creatorcontrib><creatorcontrib>Yi, Ahram</creatorcontrib><creatorcontrib>Ko, Young Jin</creatorcontrib><creatorcontrib>Kim, Serim</creatorcontrib><creatorcontrib>Kang, Seong-Ho</creatorcontrib><creatorcontrib>Park, Geon</creatorcontrib><creatorcontrib>Jang, Sook-Jin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Won, Eun Jeong</au><au>Yi, Ahram</au><au>Ko, Young Jin</au><au>Kim, Serim</au><au>Kang, Seong-Ho</au><au>Park, Geon</au><au>Jang, Sook-Jin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of Korean Pediatric Reference Intervals for Estradiol using Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry</atitle><jtitle>Clinical biochemistry</jtitle><addtitle>Clin Biochem</addtitle><date>2023-03</date><risdate>2023</risdate><volume>113</volume><spage>52</spage><epage>58</epage><pages>52-58</pages><issn>0009-9120</issn><eissn>1873-2933</eissn><abstract><![CDATA[•First established reference intervals for estradiol using a UHPLC-MS/MS-based method in South Korea.•Serum samples from 634 pediatric patients, including neonates, infants, children, and adolescents.•The highly analytical sensitive UHPLC-MS/MS method was useful for estradiol determination in children.
Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS) is a reliable and accurate method for measuring steroid hormone levels. There is an increasing need for sensitive and precise methods to measure estradiol in pediatric patients. Here, we established reference intervals for estradiol in healthy children using a UHPLC–MS/MS-based method for the first time in South Korea.
Serum estradiol was measured using a Sciex Triple QuadTM 6500 + UHPLC–MS/MS (Sciex, Framingham, MA, USA). Reference intervals for estradiol were established according to the CLSI document EP28-A3c:2008. The reference intervals were validated using serum samples from 634 pediatric patients, including neonates, children, and adolescents. Among them, 389 specimens were used in analysis of the specimen acceptance time. Statistical analysis was performed using MedCalc (MedCalc, Ostend, Belgium) and Analyse–it (Analyse–it Software Ltd., Leeds, United Kingdom) software.
Reference intervals for boys (n = 297) were <16.6, <7.3, <19.0, <30.5, 7.6–96.5, and 10.6–134.4 pmol/L among those aged <1, 1–5, 6–9, 10–11, 12–14, and 15–17 years, respectively. Reference intervals for girls (n = 337) were <114.7, <24.2, <34.8, 8.0–177.0, 10.4–480.5, and 9.1–486.7 pmol/L among those aged <1, 1–5, 6–9, 10–11, 12–14, and 15–17 years, respectively. Overall, there was no effect of specimen acceptance time on estradiol measurements in boys or girls, except for that in the group aged 10–11 years.
The reference intervals for healthy children were validated using a UHPLC–MS/MS-based method. The highly analytical sensitive UHPLC–MS/MS method may be useful for estradiol determination in pediatric patients.]]></abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>36627011</pmid><doi>10.1016/j.clinbiochem.2023.01.004</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-9120 |
| ispartof | Clinical biochemistry, 2023-03, Vol.113, p.52-58 |
| issn | 0009-9120 1873-2933 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2765772033 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adolescent Child Chromatography, High Pressure Liquid Estradiol Female High-performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS) Humans Infant, Newborn Male Reference intervals Reference Values Software Tandem Mass Spectrometry - methods |
| title | Establishment of Korean Pediatric Reference Intervals for Estradiol using Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T00%3A38%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Establishment%20of%20Korean%20Pediatric%20Reference%20Intervals%20for%20Estradiol%20using%20Ultra-High-Performance%20Liquid%20Chromatography-Tandem%20Mass%20Spectrometry&rft.jtitle=Clinical%20biochemistry&rft.au=Won,%20Eun%20Jeong&rft.date=2023-03&rft.volume=113&rft.spage=52&rft.epage=58&rft.pages=52-58&rft.issn=0009-9120&rft.eissn=1873-2933&rft_id=info:doi/10.1016/j.clinbiochem.2023.01.004&rft_dat=%3Cproquest_cross%3E2765772033%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2765772033&rft_id=info:pmid/36627011&rft_els_id=S0009912023000048&rfr_iscdi=true |