Identification of cuticle and midgut fungal microflora of phlebotomine sandflies collected in Tunisia
Phlebotomine sand flies (Diptera: Psychodidae) are the proven vectors of Leishmaniases which are widespread parasitosis in many tropical and subtropical countries. The development of infective metacyclic Leishmania (Kinetoplastida: Trypanosomatidae) promastigotes stage is restricted to the vector mi...
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| Veröffentlicht in: | Archives of microbiology 2023-02, Vol.205 (2), p.64-64, Article 64 |
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| creator | Layouni, Samia Remadi, Latifa Chaâbane-Banaoues, Raja Haouas, Najoua Babba, Hamouda |
| description | Phlebotomine sand flies (Diptera: Psychodidae) are the proven vectors of Leishmaniases which are widespread parasitosis in many tropical and subtropical countries. The development of infective metacyclic
Leishmania
(Kinetoplastida: Trypanosomatidae) promastigotes stage is restricted to the vector midgut. Recently, several studies have assessed the influence of the sand fly midgut fungal microflora on the development of invective
Leishmania
stage. The aim of this study was to identify the fungal microflora from the cuticle and midgut of wild caught sandflies. A total of 50 sandflies were caught in two different leishmaniasis foci of center Tunisia and analyzed using an
in vitro
isolation of fungi followed by a morphological and molecular identification of fungal isolates. The morphological identification of sandflies specimens revealed five Species: Phlebotomus (
P.) papatasi
(
n
= 25),
P. perniciosus
(
n
= 15)
P. riouxi
(
n
= 6),
P. longicuspis
(
n
= 3) and
P. sergenti
(
n
= 1). Forty positive fungal cultures were isolated from 34 sand flies (19 males and 15 females) distributed as following:
P. papatasi
(
n
= 16),
P. perniciosus
(
n
= 11),
P. riouxi
(
n
= 4),
P. longicuspis
(
n
= 2) and
P. sergenti
(
n
= 1). Thirty-five cultures were isolated from the cuticles and five from the guts. A total of 15 fungi genera belonging to 8 families were identified with the predominance of
Aspergillus
genus followed by
Penicillium
genus. Among the 15 fungi genera, five were common between males and females specimens.
Lecytophora canina
and
Leishmania major
co-infection was detected in the gut of a female
P. papatasi
. Our preliminary findings highlight the high diversity of fungal microflora from the sand flies midguts. |
| doi_str_mv | 10.1007/s00203-022-03386-8 |
| format | Article |
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Leishmania
(Kinetoplastida: Trypanosomatidae) promastigotes stage is restricted to the vector midgut. Recently, several studies have assessed the influence of the sand fly midgut fungal microflora on the development of invective
Leishmania
stage. The aim of this study was to identify the fungal microflora from the cuticle and midgut of wild caught sandflies. A total of 50 sandflies were caught in two different leishmaniasis foci of center Tunisia and analyzed using an
in vitro
isolation of fungi followed by a morphological and molecular identification of fungal isolates. The morphological identification of sandflies specimens revealed five Species: Phlebotomus (
P.) papatasi
(
n
= 25),
P. perniciosus
(
n
= 15)
P. riouxi
(
n
= 6),
P. longicuspis
(
n
= 3) and
P. sergenti
(
n
= 1). Forty positive fungal cultures were isolated from 34 sand flies (19 males and 15 females) distributed as following:
P. papatasi
(
n
= 16),
P. perniciosus
(
n
= 11),
P. riouxi
(
n
= 4),
P. longicuspis
(
n
= 2) and
P. sergenti
(
n
= 1). Thirty-five cultures were isolated from the cuticles and five from the guts. A total of 15 fungi genera belonging to 8 families were identified with the predominance of
Aspergillus
genus followed by
Penicillium
genus. Among the 15 fungi genera, five were common between males and females specimens.
Lecytophora canina
and
Leishmania major
co-infection was detected in the gut of a female
P. papatasi
. Our preliminary findings highlight the high diversity of fungal microflora from the sand flies midguts.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-022-03386-8</identifier><identifier>PMID: 36633698</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; Ecology ; Epicuticle ; Female ; Females ; Flies ; Fungi ; Leishmania ; Leishmaniasis ; Life Sciences ; Male ; Males ; Microbial Ecology ; Microbiology ; Microflora ; Midgut ; Morphology ; Original Paper ; Phlebotomus ; Promastigotes ; Psychodidae ; Tunisia ; Vector-borne diseases ; Vectors</subject><ispartof>Archives of microbiology, 2023-02, Vol.205 (2), p.64-64, Article 64</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c305t-eee1b01a9b827e3fcc8880e564c27129e731e1ead5fe69f8c8db546ad2fde5983</citedby><cites>FETCH-LOGICAL-c305t-eee1b01a9b827e3fcc8880e564c27129e731e1ead5fe69f8c8db546ad2fde5983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-022-03386-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-022-03386-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36633698$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Layouni, Samia</creatorcontrib><creatorcontrib>Remadi, Latifa</creatorcontrib><creatorcontrib>Chaâbane-Banaoues, Raja</creatorcontrib><creatorcontrib>Haouas, Najoua</creatorcontrib><creatorcontrib>Babba, Hamouda</creatorcontrib><title>Identification of cuticle and midgut fungal microflora of phlebotomine sandflies collected in Tunisia</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><addtitle>Arch Microbiol</addtitle><description>Phlebotomine sand flies (Diptera: Psychodidae) are the proven vectors of Leishmaniases which are widespread parasitosis in many tropical and subtropical countries. The development of infective metacyclic
Leishmania
(Kinetoplastida: Trypanosomatidae) promastigotes stage is restricted to the vector midgut. Recently, several studies have assessed the influence of the sand fly midgut fungal microflora on the development of invective
Leishmania
stage. The aim of this study was to identify the fungal microflora from the cuticle and midgut of wild caught sandflies. A total of 50 sandflies were caught in two different leishmaniasis foci of center Tunisia and analyzed using an
in vitro
isolation of fungi followed by a morphological and molecular identification of fungal isolates. The morphological identification of sandflies specimens revealed five Species: Phlebotomus (
P.) papatasi
(
n
= 25),
P. perniciosus
(
n
= 15)
P. riouxi
(
n
= 6),
P. longicuspis
(
n
= 3) and
P. sergenti
(
n
= 1). Forty positive fungal cultures were isolated from 34 sand flies (19 males and 15 females) distributed as following:
P. papatasi
(
n
= 16),
P. perniciosus
(
n
= 11),
P. riouxi
(
n
= 4),
P. longicuspis
(
n
= 2) and
P. sergenti
(
n
= 1). Thirty-five cultures were isolated from the cuticles and five from the guts. A total of 15 fungi genera belonging to 8 families were identified with the predominance of
Aspergillus
genus followed by
Penicillium
genus. Among the 15 fungi genera, five were common between males and females specimens.
Lecytophora canina
and
Leishmania major
co-infection was detected in the gut of a female
P. papatasi
. Our preliminary findings highlight the high diversity of fungal microflora from the sand flies midguts.</description><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Ecology</subject><subject>Epicuticle</subject><subject>Female</subject><subject>Females</subject><subject>Flies</subject><subject>Fungi</subject><subject>Leishmania</subject><subject>Leishmaniasis</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Males</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Microflora</subject><subject>Midgut</subject><subject>Morphology</subject><subject>Original Paper</subject><subject>Phlebotomus</subject><subject>Promastigotes</subject><subject>Psychodidae</subject><subject>Tunisia</subject><subject>Vector-borne diseases</subject><subject>Vectors</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUFPHSEUhYnR6NP2D3TRkLjpZvQCbxhm2RhbTUzcaOKOMHB5xTDwHGYW_ffyfLYmXXR1c3I_DgcOIV8YXDCA7rIAcBANcN6AEEo26oCs2FpU2fGnQ7ICAbxRvRAn5LSUZwDGlVLH5ERIKYTs1YrgrcM0Bx-smUNONHtqlznYiNQkR8fgNstM_ZI2JlZlp-xjnsyO2_6KOOQ5jyEhLZX2MWChNseIdkZHQ6IPSwolmE_kyJtY8PP7PCOPP64frm6au_uft1ff7xoroJ0bRGQDMNMPincovLU1LmAr15Z3jPfYCYYMjWs9yt4rq9zQrqVx3DtseyXOyLe973bKLwuWWY-hWIzRJMxL0byTLXTQtbyi5_-gz3mZUk23o9ZKMMmgUnxP1YeXMqHX2ymMZvqtGehdCXpfgq4l6LcS9C7F13frZRjR_T3y59crIPZAqau0wenj7v_YvgK8OZN6</recordid><startdate>20230201</startdate><enddate>20230201</enddate><creator>Layouni, Samia</creator><creator>Remadi, Latifa</creator><creator>Chaâbane-Banaoues, Raja</creator><creator>Haouas, Najoua</creator><creator>Babba, Hamouda</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20230201</creationdate><title>Identification of cuticle and midgut fungal microflora of phlebotomine sandflies collected in Tunisia</title><author>Layouni, Samia ; Remadi, Latifa ; Chaâbane-Banaoues, Raja ; Haouas, Najoua ; Babba, Hamouda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c305t-eee1b01a9b827e3fcc8880e564c27129e731e1ead5fe69f8c8db546ad2fde5983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Ecology</topic><topic>Epicuticle</topic><topic>Female</topic><topic>Females</topic><topic>Flies</topic><topic>Fungi</topic><topic>Leishmania</topic><topic>Leishmaniasis</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Males</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Microflora</topic><topic>Midgut</topic><topic>Morphology</topic><topic>Original Paper</topic><topic>Phlebotomus</topic><topic>Promastigotes</topic><topic>Psychodidae</topic><topic>Tunisia</topic><topic>Vector-borne diseases</topic><topic>Vectors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Layouni, Samia</creatorcontrib><creatorcontrib>Remadi, Latifa</creatorcontrib><creatorcontrib>Chaâbane-Banaoues, Raja</creatorcontrib><creatorcontrib>Haouas, Najoua</creatorcontrib><creatorcontrib>Babba, Hamouda</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Layouni, Samia</au><au>Remadi, Latifa</au><au>Chaâbane-Banaoues, Raja</au><au>Haouas, Najoua</au><au>Babba, Hamouda</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of cuticle and midgut fungal microflora of phlebotomine sandflies collected in Tunisia</atitle><jtitle>Archives of microbiology</jtitle><stitle>Arch Microbiol</stitle><addtitle>Arch Microbiol</addtitle><date>2023-02-01</date><risdate>2023</risdate><volume>205</volume><issue>2</issue><spage>64</spage><epage>64</epage><pages>64-64</pages><artnum>64</artnum><issn>0302-8933</issn><eissn>1432-072X</eissn><abstract>Phlebotomine sand flies (Diptera: Psychodidae) are the proven vectors of Leishmaniases which are widespread parasitosis in many tropical and subtropical countries. The development of infective metacyclic
Leishmania
(Kinetoplastida: Trypanosomatidae) promastigotes stage is restricted to the vector midgut. Recently, several studies have assessed the influence of the sand fly midgut fungal microflora on the development of invective
Leishmania
stage. The aim of this study was to identify the fungal microflora from the cuticle and midgut of wild caught sandflies. A total of 50 sandflies were caught in two different leishmaniasis foci of center Tunisia and analyzed using an
in vitro
isolation of fungi followed by a morphological and molecular identification of fungal isolates. The morphological identification of sandflies specimens revealed five Species: Phlebotomus (
P.) papatasi
(
n
= 25),
P. perniciosus
(
n
= 15)
P. riouxi
(
n
= 6),
P. longicuspis
(
n
= 3) and
P. sergenti
(
n
= 1). Forty positive fungal cultures were isolated from 34 sand flies (19 males and 15 females) distributed as following:
P. papatasi
(
n
= 16),
P. perniciosus
(
n
= 11),
P. riouxi
(
n
= 4),
P. longicuspis
(
n
= 2) and
P. sergenti
(
n
= 1). Thirty-five cultures were isolated from the cuticles and five from the guts. A total of 15 fungi genera belonging to 8 families were identified with the predominance of
Aspergillus
genus followed by
Penicillium
genus. Among the 15 fungi genera, five were common between males and females specimens.
Lecytophora canina
and
Leishmania major
co-infection was detected in the gut of a female
P. papatasi
. Our preliminary findings highlight the high diversity of fungal microflora from the sand flies midguts.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>36633698</pmid><doi>10.1007/s00203-022-03386-8</doi><tpages>1</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Archives of microbiology, 2023-02, Vol.205 (2), p.64-64, Article 64 |
| issn | 0302-8933 1432-072X |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Biochemistry Biomedical and Life Sciences Biotechnology Cell Biology Ecology Epicuticle Female Females Flies Fungi Leishmania Leishmaniasis Life Sciences Male Males Microbial Ecology Microbiology Microflora Midgut Morphology Original Paper Phlebotomus Promastigotes Psychodidae Tunisia Vector-borne diseases Vectors |
| title | Identification of cuticle and midgut fungal microflora of phlebotomine sandflies collected in Tunisia |
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