Circular RNA hsa_circ_0000519 contributes to angiogenesis and tumor progression in hepatocellular carcinoma through the miR-1296/E2F7 axis
Hepatocellular carcinoma (HCC) is the most common primary liver malignancy. Uncontrolled angiogenesis plays a critical role in hepatocellular tumor growth and metastasis. In this study, we aimed to investigate the effects of circular RNA hsa_circ_0000519 and the potential involvement of microRNA (mi...
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| Veröffentlicht in: | Human cell : official journal of Human Cell Research Society 2023-03, Vol.36 (2), p.738-751 |
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| creator | Liu, Yi Tang, Hui Zhang, Yaling Wang, Qian Li, Shiying Wang, Zhiyi Shi, Xiaofeng |
| description | Hepatocellular carcinoma (HCC) is the most common primary liver malignancy. Uncontrolled angiogenesis plays a critical role in hepatocellular tumor growth and metastasis. In this study, we aimed to investigate the effects of circular RNA hsa_circ_0000519 and the potential involvement of microRNA (miR)-1296 and E2F transcription factor 7 (E2F7) in HCC development. Hsa_circ_0000519 was highly expressed in HCC cells and hepatocellular tumor tissues, and correlated with poor prognosis of HCC patients. Knockdown of hsa_circ_0000519 significantly reduced HCC cell viability, suppressed cell proliferation, and induced cell cycle arrest in G0/G1. Downregulation of hsa_circ_0000519 also inhibited formation of capillary-like endothelial structures in vitro and impeded microvessel formation in mice bearing HCC tumors. The migration and invasive capacities of HCC cells were markedly reduced by hsa_circ_0000519 knockdown. Hsa_circ_0000519 possessed a binding site for microRNA (miR)-1296. Upregulation of hsa_circ_0000519 significantly decreased the miR-1296 expression in both HCC cells and mouse xenografts. Furthermore, E2F7 was a target of miR-1296. Hsa_circ_0000519 positively regulated E2F7 via acting as a miR-1296 sponge. Upregulation of E2F7 abolished the inhibitory effects of hsa_circ_0000519 knockdown on HCC cell proliferation and angiogenesis. In conclusion, hsa_circ_0000519 promoted tumor progression and angiogenesis in HCC through the miR-1296/E2F7 axis. These data suggest the potential clinical application of hsa_circ_0000519 in HCC treatment. |
| doi_str_mv | 10.1007/s13577-022-00854-7 |
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Uncontrolled angiogenesis plays a critical role in hepatocellular tumor growth and metastasis. In this study, we aimed to investigate the effects of circular RNA hsa_circ_0000519 and the potential involvement of microRNA (miR)-1296 and E2F transcription factor 7 (E2F7) in HCC development. Hsa_circ_0000519 was highly expressed in HCC cells and hepatocellular tumor tissues, and correlated with poor prognosis of HCC patients. Knockdown of hsa_circ_0000519 significantly reduced HCC cell viability, suppressed cell proliferation, and induced cell cycle arrest in G0/G1. Downregulation of hsa_circ_0000519 also inhibited formation of capillary-like endothelial structures in vitro and impeded microvessel formation in mice bearing HCC tumors. The migration and invasive capacities of HCC cells were markedly reduced by hsa_circ_0000519 knockdown. Hsa_circ_0000519 possessed a binding site for microRNA (miR)-1296. Upregulation of hsa_circ_0000519 significantly decreased the miR-1296 expression in both HCC cells and mouse xenografts. Furthermore, E2F7 was a target of miR-1296. Hsa_circ_0000519 positively regulated E2F7 via acting as a miR-1296 sponge. Upregulation of E2F7 abolished the inhibitory effects of hsa_circ_0000519 knockdown on HCC cell proliferation and angiogenesis. In conclusion, hsa_circ_0000519 promoted tumor progression and angiogenesis in HCC through the miR-1296/E2F7 axis. These data suggest the potential clinical application of hsa_circ_0000519 in HCC treatment.</description><identifier>ISSN: 1749-0774</identifier><identifier>ISSN: 0914-7470</identifier><identifier>EISSN: 1749-0774</identifier><identifier>DOI: 10.1007/s13577-022-00854-7</identifier><identifier>PMID: 36627545</identifier><language>eng</language><publisher>Singapore: Springer Nature Singapore</publisher><subject>Angiogenesis ; Animals ; Biomedical and Life Sciences ; Carcinoma, Hepatocellular - genetics ; Carcinoma, Hepatocellular - pathology ; Cell Biology ; Cell cycle ; Cell growth ; Cell Line, Tumor ; Cell proliferation ; Cell Proliferation - genetics ; Cell viability ; Circular RNA ; E2F protein ; E2F7 Transcription Factor - genetics ; E2F7 Transcription Factor - metabolism ; Gene Expression Regulation, Neoplastic ; Gynecology ; Hepatocellular carcinoma ; Humans ; Life Sciences ; Liver cancer ; Liver Neoplasms - genetics ; Liver Neoplasms - pathology ; Malignancy ; Metastases ; Mice ; MicroRNAs ; MicroRNAs - genetics ; miRNA ; Neovascularization, Pathologic - genetics ; Oncology ; Reproductive Medicine ; Research Article ; RNA, Circular - genetics ; Stem Cells ; Surgery ; Xenografts</subject><ispartof>Human cell : official journal of Human Cell Research Society, 2023-03, Vol.36 (2), p.738-751</ispartof><rights>The Author(s) under exclusive licence to Japan Human Cell Society 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s) under exclusive licence to Japan Human Cell Society.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-5880220e47b0b7864dc2957b3e952db32e636d7e8dd15d7ff60fdd40d124ad7b3</citedby><cites>FETCH-LOGICAL-c399t-5880220e47b0b7864dc2957b3e952db32e636d7e8dd15d7ff60fdd40d124ad7b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13577-022-00854-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13577-022-00854-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36627545$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Yi</creatorcontrib><creatorcontrib>Tang, Hui</creatorcontrib><creatorcontrib>Zhang, Yaling</creatorcontrib><creatorcontrib>Wang, Qian</creatorcontrib><creatorcontrib>Li, Shiying</creatorcontrib><creatorcontrib>Wang, Zhiyi</creatorcontrib><creatorcontrib>Shi, Xiaofeng</creatorcontrib><title>Circular RNA hsa_circ_0000519 contributes to angiogenesis and tumor progression in hepatocellular carcinoma through the miR-1296/E2F7 axis</title><title>Human cell : official journal of Human Cell Research Society</title><addtitle>Human Cell</addtitle><addtitle>Hum Cell</addtitle><description>Hepatocellular carcinoma (HCC) is the most common primary liver malignancy. Uncontrolled angiogenesis plays a critical role in hepatocellular tumor growth and metastasis. In this study, we aimed to investigate the effects of circular RNA hsa_circ_0000519 and the potential involvement of microRNA (miR)-1296 and E2F transcription factor 7 (E2F7) in HCC development. Hsa_circ_0000519 was highly expressed in HCC cells and hepatocellular tumor tissues, and correlated with poor prognosis of HCC patients. Knockdown of hsa_circ_0000519 significantly reduced HCC cell viability, suppressed cell proliferation, and induced cell cycle arrest in G0/G1. Downregulation of hsa_circ_0000519 also inhibited formation of capillary-like endothelial structures in vitro and impeded microvessel formation in mice bearing HCC tumors. The migration and invasive capacities of HCC cells were markedly reduced by hsa_circ_0000519 knockdown. Hsa_circ_0000519 possessed a binding site for microRNA (miR)-1296. Upregulation of hsa_circ_0000519 significantly decreased the miR-1296 expression in both HCC cells and mouse xenografts. Furthermore, E2F7 was a target of miR-1296. Hsa_circ_0000519 positively regulated E2F7 via acting as a miR-1296 sponge. Upregulation of E2F7 abolished the inhibitory effects of hsa_circ_0000519 knockdown on HCC cell proliferation and angiogenesis. In conclusion, hsa_circ_0000519 promoted tumor progression and angiogenesis in HCC through the miR-1296/E2F7 axis. These data suggest the potential clinical application of hsa_circ_0000519 in HCC treatment.</description><subject>Angiogenesis</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Carcinoma, Hepatocellular - genetics</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Cell Biology</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - genetics</subject><subject>Cell viability</subject><subject>Circular RNA</subject><subject>E2F protein</subject><subject>E2F7 Transcription Factor - genetics</subject><subject>E2F7 Transcription Factor - metabolism</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gynecology</subject><subject>Hepatocellular carcinoma</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Liver cancer</subject><subject>Liver Neoplasms - genetics</subject><subject>Liver Neoplasms - pathology</subject><subject>Malignancy</subject><subject>Metastases</subject><subject>Mice</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>miRNA</subject><subject>Neovascularization, Pathologic - genetics</subject><subject>Oncology</subject><subject>Reproductive Medicine</subject><subject>Research Article</subject><subject>RNA, Circular - genetics</subject><subject>Stem Cells</subject><subject>Surgery</subject><subject>Xenografts</subject><issn>1749-0774</issn><issn>0914-7470</issn><issn>1749-0774</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9uFSEUxonR2Nr6Ai4MiRs3Y4EBzsyyuWnVpLFJY9eEAWYuzR24cmYSfQWfWtpb_8RF2RwO_M7HRz5C3nD2gTMGZ8hbBdAwIRrGOiUbeEaOOci-YQDy-T_7I_IK8Y4xqaQWL8lRq7UAJdUx-bmJxa07W-jNl3O6RWtcPTCsLsV76nJaShzWJSBdMrVpinkKKWDE2ni6rHMudF_yVAJizInGRLdhb5fswm73IOxscTHl2dJlW_I6bWsNdI43DRe9PrsQl0Dt94in5MVodxheP9YTcnt58XXzqbm6_vh5c37VuLbvl0Z1Xf0xCxIGNkCnpXeiVzC0oVfCD60IutUeQuc9Vx7GUbPRe8k8F9L6yp2Q9wfdavvbGnAxc8R7tzaFvKIRoKWUAqSq6Lv_0Lu8llTdVQp6LrlgbaXEgXIlI5Ywmn2Jsy0_DGfmPilzSMpU3-YhKQN16O2j9DrMwf8Z-R1NBdoDgPUqTaH8ffsJ2V_ypp3g</recordid><startdate>20230301</startdate><enddate>20230301</enddate><creator>Liu, Yi</creator><creator>Tang, Hui</creator><creator>Zhang, Yaling</creator><creator>Wang, Qian</creator><creator>Li, Shiying</creator><creator>Wang, Zhiyi</creator><creator>Shi, Xiaofeng</creator><general>Springer Nature Singapore</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20230301</creationdate><title>Circular RNA hsa_circ_0000519 contributes to angiogenesis and tumor progression in hepatocellular carcinoma through the miR-1296/E2F7 axis</title><author>Liu, Yi ; Tang, Hui ; Zhang, Yaling ; Wang, Qian ; Li, Shiying ; Wang, Zhiyi ; Shi, Xiaofeng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-5880220e47b0b7864dc2957b3e952db32e636d7e8dd15d7ff60fdd40d124ad7b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Angiogenesis</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Carcinoma, Hepatocellular - genetics</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Cell Biology</topic><topic>Cell cycle</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - genetics</topic><topic>Cell viability</topic><topic>Circular RNA</topic><topic>E2F protein</topic><topic>E2F7 Transcription Factor - genetics</topic><topic>E2F7 Transcription Factor - metabolism</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gynecology</topic><topic>Hepatocellular carcinoma</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Liver cancer</topic><topic>Liver Neoplasms - genetics</topic><topic>Liver Neoplasms - pathology</topic><topic>Malignancy</topic><topic>Metastases</topic><topic>Mice</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>miRNA</topic><topic>Neovascularization, Pathologic - genetics</topic><topic>Oncology</topic><topic>Reproductive Medicine</topic><topic>Research Article</topic><topic>RNA, Circular - genetics</topic><topic>Stem Cells</topic><topic>Surgery</topic><topic>Xenografts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Yi</creatorcontrib><creatorcontrib>Tang, Hui</creatorcontrib><creatorcontrib>Zhang, Yaling</creatorcontrib><creatorcontrib>Wang, Qian</creatorcontrib><creatorcontrib>Li, Shiying</creatorcontrib><creatorcontrib>Wang, Zhiyi</creatorcontrib><creatorcontrib>Shi, Xiaofeng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Human cell : official journal of Human Cell Research Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Yi</au><au>Tang, Hui</au><au>Zhang, Yaling</au><au>Wang, Qian</au><au>Li, Shiying</au><au>Wang, Zhiyi</au><au>Shi, Xiaofeng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Circular RNA hsa_circ_0000519 contributes to angiogenesis and tumor progression in hepatocellular carcinoma through the miR-1296/E2F7 axis</atitle><jtitle>Human cell : official journal of Human Cell Research Society</jtitle><stitle>Human Cell</stitle><addtitle>Hum Cell</addtitle><date>2023-03-01</date><risdate>2023</risdate><volume>36</volume><issue>2</issue><spage>738</spage><epage>751</epage><pages>738-751</pages><issn>1749-0774</issn><issn>0914-7470</issn><eissn>1749-0774</eissn><abstract>Hepatocellular carcinoma (HCC) is the most common primary liver malignancy. Uncontrolled angiogenesis plays a critical role in hepatocellular tumor growth and metastasis. In this study, we aimed to investigate the effects of circular RNA hsa_circ_0000519 and the potential involvement of microRNA (miR)-1296 and E2F transcription factor 7 (E2F7) in HCC development. Hsa_circ_0000519 was highly expressed in HCC cells and hepatocellular tumor tissues, and correlated with poor prognosis of HCC patients. Knockdown of hsa_circ_0000519 significantly reduced HCC cell viability, suppressed cell proliferation, and induced cell cycle arrest in G0/G1. Downregulation of hsa_circ_0000519 also inhibited formation of capillary-like endothelial structures in vitro and impeded microvessel formation in mice bearing HCC tumors. The migration and invasive capacities of HCC cells were markedly reduced by hsa_circ_0000519 knockdown. Hsa_circ_0000519 possessed a binding site for microRNA (miR)-1296. Upregulation of hsa_circ_0000519 significantly decreased the miR-1296 expression in both HCC cells and mouse xenografts. Furthermore, E2F7 was a target of miR-1296. Hsa_circ_0000519 positively regulated E2F7 via acting as a miR-1296 sponge. Upregulation of E2F7 abolished the inhibitory effects of hsa_circ_0000519 knockdown on HCC cell proliferation and angiogenesis. In conclusion, hsa_circ_0000519 promoted tumor progression and angiogenesis in HCC through the miR-1296/E2F7 axis. These data suggest the potential clinical application of hsa_circ_0000519 in HCC treatment.</abstract><cop>Singapore</cop><pub>Springer Nature Singapore</pub><pmid>36627545</pmid><doi>10.1007/s13577-022-00854-7</doi><tpages>14</tpages></addata></record> |
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| subjects | Angiogenesis Animals Biomedical and Life Sciences Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - pathology Cell Biology Cell cycle Cell growth Cell Line, Tumor Cell proliferation Cell Proliferation - genetics Cell viability Circular RNA E2F protein E2F7 Transcription Factor - genetics E2F7 Transcription Factor - metabolism Gene Expression Regulation, Neoplastic Gynecology Hepatocellular carcinoma Humans Life Sciences Liver cancer Liver Neoplasms - genetics Liver Neoplasms - pathology Malignancy Metastases Mice MicroRNAs MicroRNAs - genetics miRNA Neovascularization, Pathologic - genetics Oncology Reproductive Medicine Research Article RNA, Circular - genetics Stem Cells Surgery Xenografts |
| title | Circular RNA hsa_circ_0000519 contributes to angiogenesis and tumor progression in hepatocellular carcinoma through the miR-1296/E2F7 axis |
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