Molecular characterization of hexokinase (HK) in Haemaphysalis longicornis and evaluation of HK protein‐ and DNA‐based vaccines against adult ticks
Background Haemaphysalis longicornis is an obligate hematophagous ectoparasite, which transmits various pathogens to humans, livestock and wild animals. Hexokinase (HK) is a key regulatory enzyme of the glycolytic pathway in the organisms. However, little is known about hexokinase and its functions...
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| Veröffentlicht in: | Pest management science 2023-05, Vol.79 (5), p.1721-1730 |
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| creator | Wang, Duo Li, Ru Wu, Ya‐Xue Fan, Xiang‐Yuan Liu, Xiao‐Ya Yang, Feng Zhang, Tian‐Tian Ma, Jing‐Yi Hu, Yong‐Hong |
| description | Background
Haemaphysalis longicornis is an obligate hematophagous ectoparasite, which transmits various pathogens to humans, livestock and wild animals. Hexokinase (HK) is a key regulatory enzyme of the glycolytic pathway in the organisms. However, little is known about hexokinase and its functions in ticks.
Results
The open reading frame of the H. longicornis HK (HlHK) gene was 1425 bp and encoded a protein of 474 amino acids, containing conserved domains for glucose, glucose 6‐phosphate, and adenosine triphosphate. The expression of HlHK gene was detected at different developmental stages and in different tissues of unfed female ticks. Enzyme‐linked immunosorbent assay revealed that both HK protein‐ and DNA‐based vaccines increased the antibody levels of the immunized animals. A vaccination trail on rabbits against H. longicornis infestation indicated that the rHlHK protein and HlHK DNA vaccines reduced the number of attached female ticks by 9% and 12%, egg mass weight by 36% and 34%, and egg hatching rate by 41% and 17%, respectively. Overall, protein vaccination conferred 65.6% protection against adult female ticks, whereas the DNA vaccine conferred 51.8% protection.
Conclusion
This is the first report of the molecular characterization of the HK protein and sequencing of the HK gene from H. longicornis. Positive results from vaccination trials on rabbits of the recombinant HK protein and HK DNA suggest that these novel anti‐tick vaccines potentially can be used as viable tick control tools for the management of the Asian longhorned tick. Additionally, inhibition of glucose metabolism may be a new strategy for tick control. © 2023 Society of Chemical Industry.
This is the first report of the molecular characterization of the HK protein from Haemaphysali longicornis. Results of vaccination trials on rabbits suggest that HK protein‐ and DNA‐based vaccines, through inhibiting glucose metabolism, potentially can be used as viable tick control tools for the management of the Asian longhorned tick. |
| doi_str_mv | 10.1002/ps.7346 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2761987396</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2793373052</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3456-62486c54af7825183bcd977fba36cbb8ef0537c064e75e25d6b0c1f2922f7823</originalsourceid><addsrcrecordid>eNp1kctu1DAUhi0EoqUg3gBZYkERmuLYiR0vq3KZquUi0QW76MQ56bj12KmdFIYVj8CO9-NJ6umUWSCx8i_5-z-do0PI04IdFIzx10M6UKKU98huUXE5K7Wu729z_XWHPErpgjGmteYPyY6QksmSyV3y-0NwaCYHkZoFRDAjRvsDRhs8DT1d4PdwaT0kpPvzk5fUejoHXMKwWCVwNlEX_Lk1IfqcwXcUr8FN2_r8hA4xjGj9n5-_bv_ffDzMsc3Cjl6DMdZjLp6D9Wmk0E1upKM1l-kxedCDS_jk7t0jZ-_enh3NZ6ef3h8fHZ7OjCgrOZO8rKWpSuhVzauiFq3ptFJ9C0Katq2xZ5VQJi-LqkJedbJlpui55nzdEHtkf6PNY15NmMZmaZNB58BjmFLDlSx0rYSWGX3-D3oRpujzcJnSQijBqrXwxYYyMaQUsW-GaJcQV03BmvWpmiE161Nl8tmdb2qX2G25v7fJwKsN8M06XP3P03z-cqu7AdcRnvI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2793373052</pqid></control><display><type>article</type><title>Molecular characterization of hexokinase (HK) in Haemaphysalis longicornis and evaluation of HK protein‐ and DNA‐based vaccines against adult ticks</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Wang, Duo ; Li, Ru ; Wu, Ya‐Xue ; Fan, Xiang‐Yuan ; Liu, Xiao‐Ya ; Yang, Feng ; Zhang, Tian‐Tian ; Ma, Jing‐Yi ; Hu, Yong‐Hong</creator><creatorcontrib>Wang, Duo ; Li, Ru ; Wu, Ya‐Xue ; Fan, Xiang‐Yuan ; Liu, Xiao‐Ya ; Yang, Feng ; Zhang, Tian‐Tian ; Ma, Jing‐Yi ; Hu, Yong‐Hong</creatorcontrib><description>Background
Haemaphysalis longicornis is an obligate hematophagous ectoparasite, which transmits various pathogens to humans, livestock and wild animals. Hexokinase (HK) is a key regulatory enzyme of the glycolytic pathway in the organisms. However, little is known about hexokinase and its functions in ticks.
Results
The open reading frame of the H. longicornis HK (HlHK) gene was 1425 bp and encoded a protein of 474 amino acids, containing conserved domains for glucose, glucose 6‐phosphate, and adenosine triphosphate. The expression of HlHK gene was detected at different developmental stages and in different tissues of unfed female ticks. Enzyme‐linked immunosorbent assay revealed that both HK protein‐ and DNA‐based vaccines increased the antibody levels of the immunized animals. A vaccination trail on rabbits against H. longicornis infestation indicated that the rHlHK protein and HlHK DNA vaccines reduced the number of attached female ticks by 9% and 12%, egg mass weight by 36% and 34%, and egg hatching rate by 41% and 17%, respectively. Overall, protein vaccination conferred 65.6% protection against adult female ticks, whereas the DNA vaccine conferred 51.8% protection.
Conclusion
This is the first report of the molecular characterization of the HK protein and sequencing of the HK gene from H. longicornis. Positive results from vaccination trials on rabbits of the recombinant HK protein and HK DNA suggest that these novel anti‐tick vaccines potentially can be used as viable tick control tools for the management of the Asian longhorned tick. Additionally, inhibition of glucose metabolism may be a new strategy for tick control. © 2023 Society of Chemical Industry.
This is the first report of the molecular characterization of the HK protein from Haemaphysali longicornis. Results of vaccination trials on rabbits suggest that HK protein‐ and DNA‐based vaccines, through inhibiting glucose metabolism, potentially can be used as viable tick control tools for the management of the Asian longhorned tick.</description><identifier>ISSN: 1526-498X</identifier><identifier>EISSN: 1526-4998</identifier><identifier>DOI: 10.1002/ps.7346</identifier><identifier>PMID: 36606406</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Adenosine triphosphate ; Amino acids ; Animals ; Antibodies ; ATP ; Deoxyribonucleic acid ; Developmental stages ; DNA ; DNA vaccine ; DNA vaccines ; Ectoparasites ; Eggs ; Enzymes ; Female ; Females ; Gene expression ; Glucose ; Glucose metabolism ; Glycolysis ; Haemaphysalis longicornis ; Hatching ; Hexokinase ; Hexokinase - genetics ; Hexokinase - metabolism ; Hk gene ; Humans ; Immunization ; Ixodidae - genetics ; Livestock ; protein vaccine ; Proteins ; Rabbits ; Ticks ; Vaccines ; Vaccines, DNA - metabolism ; Wild animals</subject><ispartof>Pest management science, 2023-05, Vol.79 (5), p.1721-1730</ispartof><rights>2023 Society of Chemical Industry.</rights><rights>Copyright © 2023 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3456-62486c54af7825183bcd977fba36cbb8ef0537c064e75e25d6b0c1f2922f7823</citedby><cites>FETCH-LOGICAL-c3456-62486c54af7825183bcd977fba36cbb8ef0537c064e75e25d6b0c1f2922f7823</cites><orcidid>0000-0002-1924-1002 ; 0000-0001-5506-072X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fps.7346$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fps.7346$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36606406$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Duo</creatorcontrib><creatorcontrib>Li, Ru</creatorcontrib><creatorcontrib>Wu, Ya‐Xue</creatorcontrib><creatorcontrib>Fan, Xiang‐Yuan</creatorcontrib><creatorcontrib>Liu, Xiao‐Ya</creatorcontrib><creatorcontrib>Yang, Feng</creatorcontrib><creatorcontrib>Zhang, Tian‐Tian</creatorcontrib><creatorcontrib>Ma, Jing‐Yi</creatorcontrib><creatorcontrib>Hu, Yong‐Hong</creatorcontrib><title>Molecular characterization of hexokinase (HK) in Haemaphysalis longicornis and evaluation of HK protein‐ and DNA‐based vaccines against adult ticks</title><title>Pest management science</title><addtitle>Pest Manag Sci</addtitle><description>Background
Haemaphysalis longicornis is an obligate hematophagous ectoparasite, which transmits various pathogens to humans, livestock and wild animals. Hexokinase (HK) is a key regulatory enzyme of the glycolytic pathway in the organisms. However, little is known about hexokinase and its functions in ticks.
Results
The open reading frame of the H. longicornis HK (HlHK) gene was 1425 bp and encoded a protein of 474 amino acids, containing conserved domains for glucose, glucose 6‐phosphate, and adenosine triphosphate. The expression of HlHK gene was detected at different developmental stages and in different tissues of unfed female ticks. Enzyme‐linked immunosorbent assay revealed that both HK protein‐ and DNA‐based vaccines increased the antibody levels of the immunized animals. A vaccination trail on rabbits against H. longicornis infestation indicated that the rHlHK protein and HlHK DNA vaccines reduced the number of attached female ticks by 9% and 12%, egg mass weight by 36% and 34%, and egg hatching rate by 41% and 17%, respectively. Overall, protein vaccination conferred 65.6% protection against adult female ticks, whereas the DNA vaccine conferred 51.8% protection.
Conclusion
This is the first report of the molecular characterization of the HK protein and sequencing of the HK gene from H. longicornis. Positive results from vaccination trials on rabbits of the recombinant HK protein and HK DNA suggest that these novel anti‐tick vaccines potentially can be used as viable tick control tools for the management of the Asian longhorned tick. Additionally, inhibition of glucose metabolism may be a new strategy for tick control. © 2023 Society of Chemical Industry.
This is the first report of the molecular characterization of the HK protein from Haemaphysali longicornis. Results of vaccination trials on rabbits suggest that HK protein‐ and DNA‐based vaccines, through inhibiting glucose metabolism, potentially can be used as viable tick control tools for the management of the Asian longhorned tick.</description><subject>Adenosine triphosphate</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Antibodies</subject><subject>ATP</subject><subject>Deoxyribonucleic acid</subject><subject>Developmental stages</subject><subject>DNA</subject><subject>DNA vaccine</subject><subject>DNA vaccines</subject><subject>Ectoparasites</subject><subject>Eggs</subject><subject>Enzymes</subject><subject>Female</subject><subject>Females</subject><subject>Gene expression</subject><subject>Glucose</subject><subject>Glucose metabolism</subject><subject>Glycolysis</subject><subject>Haemaphysalis longicornis</subject><subject>Hatching</subject><subject>Hexokinase</subject><subject>Hexokinase - genetics</subject><subject>Hexokinase - metabolism</subject><subject>Hk gene</subject><subject>Humans</subject><subject>Immunization</subject><subject>Ixodidae - genetics</subject><subject>Livestock</subject><subject>protein vaccine</subject><subject>Proteins</subject><subject>Rabbits</subject><subject>Ticks</subject><subject>Vaccines</subject><subject>Vaccines, DNA - metabolism</subject><subject>Wild animals</subject><issn>1526-498X</issn><issn>1526-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctu1DAUhi0EoqUg3gBZYkERmuLYiR0vq3KZquUi0QW76MQ56bj12KmdFIYVj8CO9-NJ6umUWSCx8i_5-z-do0PI04IdFIzx10M6UKKU98huUXE5K7Wu729z_XWHPErpgjGmteYPyY6QksmSyV3y-0NwaCYHkZoFRDAjRvsDRhs8DT1d4PdwaT0kpPvzk5fUejoHXMKwWCVwNlEX_Lk1IfqcwXcUr8FN2_r8hA4xjGj9n5-_bv_ffDzMsc3Cjl6DMdZjLp6D9Wmk0E1upKM1l-kxedCDS_jk7t0jZ-_enh3NZ6ef3h8fHZ7OjCgrOZO8rKWpSuhVzauiFq3ptFJ9C0Katq2xZ5VQJi-LqkJedbJlpui55nzdEHtkf6PNY15NmMZmaZNB58BjmFLDlSx0rYSWGX3-D3oRpujzcJnSQijBqrXwxYYyMaQUsW-GaJcQV03BmvWpmiE161Nl8tmdb2qX2G25v7fJwKsN8M06XP3P03z-cqu7AdcRnvI</recordid><startdate>202305</startdate><enddate>202305</enddate><creator>Wang, Duo</creator><creator>Li, Ru</creator><creator>Wu, Ya‐Xue</creator><creator>Fan, Xiang‐Yuan</creator><creator>Liu, Xiao‐Ya</creator><creator>Yang, Feng</creator><creator>Zhang, Tian‐Tian</creator><creator>Ma, Jing‐Yi</creator><creator>Hu, Yong‐Hong</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1924-1002</orcidid><orcidid>https://orcid.org/0000-0001-5506-072X</orcidid></search><sort><creationdate>202305</creationdate><title>Molecular characterization of hexokinase (HK) in Haemaphysalis longicornis and evaluation of HK protein‐ and DNA‐based vaccines against adult ticks</title><author>Wang, Duo ; Li, Ru ; Wu, Ya‐Xue ; Fan, Xiang‐Yuan ; Liu, Xiao‐Ya ; Yang, Feng ; Zhang, Tian‐Tian ; Ma, Jing‐Yi ; Hu, Yong‐Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3456-62486c54af7825183bcd977fba36cbb8ef0537c064e75e25d6b0c1f2922f7823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Adenosine triphosphate</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Antibodies</topic><topic>ATP</topic><topic>Deoxyribonucleic acid</topic><topic>Developmental stages</topic><topic>DNA</topic><topic>DNA vaccine</topic><topic>DNA vaccines</topic><topic>Ectoparasites</topic><topic>Eggs</topic><topic>Enzymes</topic><topic>Female</topic><topic>Females</topic><topic>Gene expression</topic><topic>Glucose</topic><topic>Glucose metabolism</topic><topic>Glycolysis</topic><topic>Haemaphysalis longicornis</topic><topic>Hatching</topic><topic>Hexokinase</topic><topic>Hexokinase - genetics</topic><topic>Hexokinase - metabolism</topic><topic>Hk gene</topic><topic>Humans</topic><topic>Immunization</topic><topic>Ixodidae - genetics</topic><topic>Livestock</topic><topic>protein vaccine</topic><topic>Proteins</topic><topic>Rabbits</topic><topic>Ticks</topic><topic>Vaccines</topic><topic>Vaccines, DNA - metabolism</topic><topic>Wild animals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Duo</creatorcontrib><creatorcontrib>Li, Ru</creatorcontrib><creatorcontrib>Wu, Ya‐Xue</creatorcontrib><creatorcontrib>Fan, Xiang‐Yuan</creatorcontrib><creatorcontrib>Liu, Xiao‐Ya</creatorcontrib><creatorcontrib>Yang, Feng</creatorcontrib><creatorcontrib>Zhang, Tian‐Tian</creatorcontrib><creatorcontrib>Ma, Jing‐Yi</creatorcontrib><creatorcontrib>Hu, Yong‐Hong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Pest management science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Duo</au><au>Li, Ru</au><au>Wu, Ya‐Xue</au><au>Fan, Xiang‐Yuan</au><au>Liu, Xiao‐Ya</au><au>Yang, Feng</au><au>Zhang, Tian‐Tian</au><au>Ma, Jing‐Yi</au><au>Hu, Yong‐Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of hexokinase (HK) in Haemaphysalis longicornis and evaluation of HK protein‐ and DNA‐based vaccines against adult ticks</atitle><jtitle>Pest management science</jtitle><addtitle>Pest Manag Sci</addtitle><date>2023-05</date><risdate>2023</risdate><volume>79</volume><issue>5</issue><spage>1721</spage><epage>1730</epage><pages>1721-1730</pages><issn>1526-498X</issn><eissn>1526-4998</eissn><abstract>Background
Haemaphysalis longicornis is an obligate hematophagous ectoparasite, which transmits various pathogens to humans, livestock and wild animals. Hexokinase (HK) is a key regulatory enzyme of the glycolytic pathway in the organisms. However, little is known about hexokinase and its functions in ticks.
Results
The open reading frame of the H. longicornis HK (HlHK) gene was 1425 bp and encoded a protein of 474 amino acids, containing conserved domains for glucose, glucose 6‐phosphate, and adenosine triphosphate. The expression of HlHK gene was detected at different developmental stages and in different tissues of unfed female ticks. Enzyme‐linked immunosorbent assay revealed that both HK protein‐ and DNA‐based vaccines increased the antibody levels of the immunized animals. A vaccination trail on rabbits against H. longicornis infestation indicated that the rHlHK protein and HlHK DNA vaccines reduced the number of attached female ticks by 9% and 12%, egg mass weight by 36% and 34%, and egg hatching rate by 41% and 17%, respectively. Overall, protein vaccination conferred 65.6% protection against adult female ticks, whereas the DNA vaccine conferred 51.8% protection.
Conclusion
This is the first report of the molecular characterization of the HK protein and sequencing of the HK gene from H. longicornis. Positive results from vaccination trials on rabbits of the recombinant HK protein and HK DNA suggest that these novel anti‐tick vaccines potentially can be used as viable tick control tools for the management of the Asian longhorned tick. Additionally, inhibition of glucose metabolism may be a new strategy for tick control. © 2023 Society of Chemical Industry.
This is the first report of the molecular characterization of the HK protein from Haemaphysali longicornis. Results of vaccination trials on rabbits suggest that HK protein‐ and DNA‐based vaccines, through inhibiting glucose metabolism, potentially can be used as viable tick control tools for the management of the Asian longhorned tick.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>36606406</pmid><doi>10.1002/ps.7346</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-1924-1002</orcidid><orcidid>https://orcid.org/0000-0001-5506-072X</orcidid></addata></record> |
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| subjects | Adenosine triphosphate Amino acids Animals Antibodies ATP Deoxyribonucleic acid Developmental stages DNA DNA vaccine DNA vaccines Ectoparasites Eggs Enzymes Female Females Gene expression Glucose Glucose metabolism Glycolysis Haemaphysalis longicornis Hatching Hexokinase Hexokinase - genetics Hexokinase - metabolism Hk gene Humans Immunization Ixodidae - genetics Livestock protein vaccine Proteins Rabbits Ticks Vaccines Vaccines, DNA - metabolism Wild animals |
| title | Molecular characterization of hexokinase (HK) in Haemaphysalis longicornis and evaluation of HK protein‐ and DNA‐based vaccines against adult ticks |
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