Rapid detection of monkeypox virus by multiple cross displacement amplification combined with nanoparticle‐based biosensor platform
The current outbreak of monkeypox virus (MPXV) has become a public health emergency of international concern that highlights the need for rapid, sensitive MPXV diagnostic assays. Here, we combined isothermal multiple cross displacement amplification (MCDA) with nanoparticle‐based lateral flow biosen...
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| Veröffentlicht in: | Journal of medical virology 2023-02, Vol.95 (2), p.e28479-n/a |
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| creator | Zhou, Juan Xiao, Fei Fu, Jin Jia, Nan Huang, Xiaolan Sun, Chunrong Liu, Chunyan Huan, Hui Wang, Yi |
| description | The current outbreak of monkeypox virus (MPXV) has become a public health emergency of international concern that highlights the need for rapid, sensitive MPXV diagnostic assays. Here, we combined isothermal multiple cross displacement amplification (MCDA) with nanoparticle‐based lateral flow biosensor (LFB) to devise a diagnostic test for the diagnosis of MPXV infection (called MPXV‐MCDA‐LFB) and differentiation of West and Central African MPXV isolates. The MPXV‐MCDA‐LFB protocol conducts isothermal MCDA reaction for DNA templates followed by LFB detection of preamplification target sequences. Two MCDA primer sets were designed targeting the D41L and ATI genes of Central and West African MPXV isolates, respectively, and the optimal condition of two MCDA reactions was 64°C for 30 min. The two MCDA reactions were decoded by LFB, which was devised for detecting three targets, including two amplicons yielded from two MCDA reactions and a chromatography control. Thus, the MPXV‐MCDA‐LFB assay could be completed within 50 min including rapid template preparation (15 min), MCDA reaction (30 min) and reporting of result ( |
| doi_str_mv | 10.1002/jmv.28479 |
| format | Article |
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Here, we combined isothermal multiple cross displacement amplification (MCDA) with nanoparticle‐based lateral flow biosensor (LFB) to devise a diagnostic test for the diagnosis of MPXV infection (called MPXV‐MCDA‐LFB) and differentiation of West and Central African MPXV isolates. The MPXV‐MCDA‐LFB protocol conducts isothermal MCDA reaction for DNA templates followed by LFB detection of preamplification target sequences. Two MCDA primer sets were designed targeting the D41L and ATI genes of Central and West African MPXV isolates, respectively, and the optimal condition of two MCDA reactions was 64°C for 30 min. The two MCDA reactions were decoded by LFB, which was devised for detecting three targets, including two amplicons yielded from two MCDA reactions and a chromatography control. Thus, the MPXV‐MCDA‐LFB assay could be completed within 50 min including rapid template preparation (15 min), MCDA reaction (30 min) and reporting of result (<5 min). The MPXV‐MCDA‐LFB method is very sensitive and can detect the target genes (D14L and ATI) with as low as five copies of plasmid template per reaction and 12.5 copies of pseudotyped virus in human blood samples. The MPXV‐MCDA‐LFB assay does not cross‐react with non‐MPXV templates, validating its specificity. Therefore, the MPXV‐MCDA‐LFB assay developed here is a useful tool for rapid and reliable diagnosis of MPXV infection.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.28479</identifier><identifier>PMID: 36609918</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Amplification ; Assaying ; Biosensing Techniques - methods ; biosensor ; Biosensors ; Deoxyribonucleic acid ; Diagnosis ; DNA ; Genes ; Humans ; Infections ; lateral flow platform ; Monkeypox ; Monkeypox virus ; Mpox (monkeypox) ; MPXV infection ; multiple cross displacement amplification ; Nanoparticles ; Nanoparticles - chemistry ; Nucleic Acid Amplification Techniques - methods ; Nucleotide sequence ; Public health ; Sensitivity and Specificity ; Target detection ; Virology ; Viruses</subject><ispartof>Journal of medical virology, 2023-02, Vol.95 (2), p.e28479-n/a</ispartof><rights>2023 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3889-7815c1f5f035610a6b5846c6cd57e26df19a3d3bda4dd69ba664a6db33b1a0c43</citedby><cites>FETCH-LOGICAL-c3889-7815c1f5f035610a6b5846c6cd57e26df19a3d3bda4dd69ba664a6db33b1a0c43</cites><orcidid>0000-0002-2031-087X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmv.28479$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmv.28479$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36609918$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Juan</creatorcontrib><creatorcontrib>Xiao, Fei</creatorcontrib><creatorcontrib>Fu, Jin</creatorcontrib><creatorcontrib>Jia, Nan</creatorcontrib><creatorcontrib>Huang, Xiaolan</creatorcontrib><creatorcontrib>Sun, Chunrong</creatorcontrib><creatorcontrib>Liu, Chunyan</creatorcontrib><creatorcontrib>Huan, Hui</creatorcontrib><creatorcontrib>Wang, Yi</creatorcontrib><title>Rapid detection of monkeypox virus by multiple cross displacement amplification combined with nanoparticle‐based biosensor platform</title><title>Journal of medical virology</title><addtitle>J Med Virol</addtitle><description>The current outbreak of monkeypox virus (MPXV) has become a public health emergency of international concern that highlights the need for rapid, sensitive MPXV diagnostic assays. Here, we combined isothermal multiple cross displacement amplification (MCDA) with nanoparticle‐based lateral flow biosensor (LFB) to devise a diagnostic test for the diagnosis of MPXV infection (called MPXV‐MCDA‐LFB) and differentiation of West and Central African MPXV isolates. The MPXV‐MCDA‐LFB protocol conducts isothermal MCDA reaction for DNA templates followed by LFB detection of preamplification target sequences. Two MCDA primer sets were designed targeting the D41L and ATI genes of Central and West African MPXV isolates, respectively, and the optimal condition of two MCDA reactions was 64°C for 30 min. The two MCDA reactions were decoded by LFB, which was devised for detecting three targets, including two amplicons yielded from two MCDA reactions and a chromatography control. Thus, the MPXV‐MCDA‐LFB assay could be completed within 50 min including rapid template preparation (15 min), MCDA reaction (30 min) and reporting of result (<5 min). The MPXV‐MCDA‐LFB method is very sensitive and can detect the target genes (D14L and ATI) with as low as five copies of plasmid template per reaction and 12.5 copies of pseudotyped virus in human blood samples. The MPXV‐MCDA‐LFB assay does not cross‐react with non‐MPXV templates, validating its specificity. Therefore, the MPXV‐MCDA‐LFB assay developed here is a useful tool for rapid and reliable diagnosis of MPXV infection.</description><subject>Amplification</subject><subject>Assaying</subject><subject>Biosensing Techniques - methods</subject><subject>biosensor</subject><subject>Biosensors</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnosis</subject><subject>DNA</subject><subject>Genes</subject><subject>Humans</subject><subject>Infections</subject><subject>lateral flow platform</subject><subject>Monkeypox</subject><subject>Monkeypox virus</subject><subject>Mpox (monkeypox)</subject><subject>MPXV infection</subject><subject>multiple cross displacement amplification</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Nucleic Acid Amplification Techniques - methods</subject><subject>Nucleotide sequence</subject><subject>Public health</subject><subject>Sensitivity and Specificity</subject><subject>Target detection</subject><subject>Virology</subject><subject>Viruses</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kT1v1TAUQK0KRB-FoX8AWWKhQ1o7cRx7RBUUUFGlClgjf9wIv_ojtZOWt7Gw8xv5JaTvtR2QmDzcc4-ufBA6pOSYElKfrMPNcS1YJ_fQihLJK0k6-gStCGW84py2--h5KWtCiJB1_QztN5wTKalYoV-XanQWW5jATC5FnAYcUryCzZh-4BuX54L1BofZT270gE1OpWDryuiVgQBxwiqM3g3OqO2-SUG7CBbfuuk7jiqmUeXJGQ9_fv7WqiwT7VKBWFLGi2QaUg4v0NNB-QIv798D9PX9uy-nH6rzi7OPp2_PK9MIIatO0NbQoR1I03JKFNetYNxwY9sOam4HKlVjG20Vs5ZLrThnilvdNJoqYlhzgN7svGNO1zOUqQ-uGPBeRUhz6euOUylYS-_Q1_-g6zTnuFy3UJ1kdc1Et1BHO2r7LxmGfswuqLzpKenv2vRLm37bZmFf3RtnHcA-kg8xFuBkB9w6D5v_m_pPn7_tlH8BBAGc_g</recordid><startdate>202302</startdate><enddate>202302</enddate><creator>Zhou, Juan</creator><creator>Xiao, Fei</creator><creator>Fu, Jin</creator><creator>Jia, Nan</creator><creator>Huang, Xiaolan</creator><creator>Sun, Chunrong</creator><creator>Liu, Chunyan</creator><creator>Huan, Hui</creator><creator>Wang, Yi</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2031-087X</orcidid></search><sort><creationdate>202302</creationdate><title>Rapid detection of monkeypox virus by multiple cross displacement amplification combined with nanoparticle‐based biosensor platform</title><author>Zhou, Juan ; Xiao, Fei ; Fu, Jin ; Jia, Nan ; Huang, Xiaolan ; Sun, Chunrong ; Liu, Chunyan ; Huan, Hui ; Wang, Yi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3889-7815c1f5f035610a6b5846c6cd57e26df19a3d3bda4dd69ba664a6db33b1a0c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Amplification</topic><topic>Assaying</topic><topic>Biosensing Techniques - methods</topic><topic>biosensor</topic><topic>Biosensors</topic><topic>Deoxyribonucleic acid</topic><topic>Diagnosis</topic><topic>DNA</topic><topic>Genes</topic><topic>Humans</topic><topic>Infections</topic><topic>lateral flow platform</topic><topic>Monkeypox</topic><topic>Monkeypox virus</topic><topic>Mpox (monkeypox)</topic><topic>MPXV infection</topic><topic>multiple cross displacement amplification</topic><topic>Nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>Nucleic Acid Amplification Techniques - methods</topic><topic>Nucleotide sequence</topic><topic>Public health</topic><topic>Sensitivity and Specificity</topic><topic>Target detection</topic><topic>Virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Juan</creatorcontrib><creatorcontrib>Xiao, Fei</creatorcontrib><creatorcontrib>Fu, Jin</creatorcontrib><creatorcontrib>Jia, Nan</creatorcontrib><creatorcontrib>Huang, Xiaolan</creatorcontrib><creatorcontrib>Sun, Chunrong</creatorcontrib><creatorcontrib>Liu, Chunyan</creatorcontrib><creatorcontrib>Huan, Hui</creatorcontrib><creatorcontrib>Wang, Yi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Juan</au><au>Xiao, Fei</au><au>Fu, Jin</au><au>Jia, Nan</au><au>Huang, Xiaolan</au><au>Sun, Chunrong</au><au>Liu, Chunyan</au><au>Huan, Hui</au><au>Wang, Yi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid detection of monkeypox virus by multiple cross displacement amplification combined with nanoparticle‐based biosensor platform</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J Med Virol</addtitle><date>2023-02</date><risdate>2023</risdate><volume>95</volume><issue>2</issue><spage>e28479</spage><epage>n/a</epage><pages>e28479-n/a</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><abstract>The current outbreak of monkeypox virus (MPXV) has become a public health emergency of international concern that highlights the need for rapid, sensitive MPXV diagnostic assays. Here, we combined isothermal multiple cross displacement amplification (MCDA) with nanoparticle‐based lateral flow biosensor (LFB) to devise a diagnostic test for the diagnosis of MPXV infection (called MPXV‐MCDA‐LFB) and differentiation of West and Central African MPXV isolates. The MPXV‐MCDA‐LFB protocol conducts isothermal MCDA reaction for DNA templates followed by LFB detection of preamplification target sequences. Two MCDA primer sets were designed targeting the D41L and ATI genes of Central and West African MPXV isolates, respectively, and the optimal condition of two MCDA reactions was 64°C for 30 min. The two MCDA reactions were decoded by LFB, which was devised for detecting three targets, including two amplicons yielded from two MCDA reactions and a chromatography control. Thus, the MPXV‐MCDA‐LFB assay could be completed within 50 min including rapid template preparation (15 min), MCDA reaction (30 min) and reporting of result (<5 min). The MPXV‐MCDA‐LFB method is very sensitive and can detect the target genes (D14L and ATI) with as low as five copies of plasmid template per reaction and 12.5 copies of pseudotyped virus in human blood samples. The MPXV‐MCDA‐LFB assay does not cross‐react with non‐MPXV templates, validating its specificity. Therefore, the MPXV‐MCDA‐LFB assay developed here is a useful tool for rapid and reliable diagnosis of MPXV infection.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>36609918</pmid><doi>10.1002/jmv.28479</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-2031-087X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Amplification Assaying Biosensing Techniques - methods biosensor Biosensors Deoxyribonucleic acid Diagnosis DNA Genes Humans Infections lateral flow platform Monkeypox Monkeypox virus Mpox (monkeypox) MPXV infection multiple cross displacement amplification Nanoparticles Nanoparticles - chemistry Nucleic Acid Amplification Techniques - methods Nucleotide sequence Public health Sensitivity and Specificity Target detection Virology Viruses |
| title | Rapid detection of monkeypox virus by multiple cross displacement amplification combined with nanoparticle‐based biosensor platform |
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