A preliminary study on placental damage associated to experimental neosporosis in BALB/c mice
Neospora caninum is a protozoan parasite which can infect a range of animals, including dogs, cattle, and sheep. Bovine neosporosis, which mainly causes abortion in cattle, results in substantial economic losses worldwide. To study the effects of N. caninum infection on the placenta, a pregnant mous...
Gespeichert in:
| Veröffentlicht in: | Parasitology research (1987) 2023-03, Vol.122 (3), p.781-788 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 788 |
|---|---|
| container_issue | 3 |
| container_start_page | 781 |
| container_title | Parasitology research (1987) |
| container_volume | 122 |
| creator | Tang, Zeyu Li, Hang Xie, Suzhu Zhao, Shaowei Zhang, Shuang Wang, Hao Li, Nanli Zhang, Xuancheng Zhao, Fanglin Jia, Lijun |
| description | Neospora caninum
is a protozoan parasite which can infect a range of animals, including dogs, cattle, and sheep. Bovine neosporosis, which mainly causes abortion in cattle, results in substantial economic losses worldwide. To study the effects of
N. caninum
infection on the placenta, a pregnant mouse model for
N. caninum
infection was established. The litter size (8.6 ± 1.5) and the number of live pups (6.4 ± 1.8) of infected dams were significantly lower compared with those of non-infected dams. Trophoblast cell shrinkage and a large number of apoptosomes were detected in the placentas of the infected group. The parasite load in the placental tissue was significantly higher with time after infection. Likewise, apoptosis of placental trophoblast cells significantly increased with time after infection. Among the 66 apoptotic genes detected in this study, eight genes, including
Bcl
-
2
, were significantly differentially expressed by about > tenfold in infected and uninfected mice. The expression of BAX and tumor necrosis factor-alpha (TNF-α) was upregulated in the placental cells of the infected mice, whereas the expression of BCL-2 was downregulated. Enzyme-linked immunosorbent assays (ELISAs) showed that apoptotic protease caspase-3 level was significantly increased in placental cell suspension, and insulin-like growth factor (IGF)-2 level was significantly reduced. Acetylcholine (ACH) and placental prolactin (PL) levels were initially decreased but eventually increased. In summary, infection of mice with
N. caninum
caused apoptotic damage to the placental tissues, cells, and genes and affected the normal physiological functions of placenta, which may largely explain the adverse pregnancy outcomes caused by
N. caninum
infection in mice. |
| doi_str_mv | 10.1007/s00436-022-07771-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2760817201</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2760817201</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-292f7702c35480a4ef8161bc4de6c0d27034f6fd31269f2f8e6f877ec910db53</originalsourceid><addsrcrecordid>eNp9kE1LAzEQhoMoWqt_wIMEvHhZO0l2k91jW_yCgpdeJaTZSVnZLze7YP-9qVsVPHjKQJ55Z-Yh5IrBHQNQMw8QCxkB5xEopVgkj8iExYJHLEuSYzKBLNTAmDgj596_ATAl4_iUnAmZZDIDPiGvc9p2WBZVUZtuR30_5Dva1LQtjcW6NyXNTWW2SI33jS1MjzntG4ofLXZFNRI1Nr5tusYXnhY1XcxXi5mlVWHxgpw4U3q8PLxTsn64Xy-fotXL4_NyvoqsUEkf8Yw7pYBbkcQpmBhdyiTb2DhHaSHnCkTspMsF4zJz3KUoXaoU2oxBvknElNyOsW3XvA_oe10V3mJZmrDa4DVXElKmOLCA3vxB35qhq8NygUpFmqUA-0A-UjZc5Tt0ug3XBkGagd6716N7HdzrL_dahqbrQ_SwqTD_afmWHQAxAj581Vvsfmf_E_sJYDiOOw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2783898005</pqid></control><display><type>article</type><title>A preliminary study on placental damage associated to experimental neosporosis in BALB/c mice</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Tang, Zeyu ; Li, Hang ; Xie, Suzhu ; Zhao, Shaowei ; Zhang, Shuang ; Wang, Hao ; Li, Nanli ; Zhang, Xuancheng ; Zhao, Fanglin ; Jia, Lijun</creator><creatorcontrib>Tang, Zeyu ; Li, Hang ; Xie, Suzhu ; Zhao, Shaowei ; Zhang, Shuang ; Wang, Hao ; Li, Nanli ; Zhang, Xuancheng ; Zhao, Fanglin ; Jia, Lijun</creatorcontrib><description>Neospora caninum
is a protozoan parasite which can infect a range of animals, including dogs, cattle, and sheep. Bovine neosporosis, which mainly causes abortion in cattle, results in substantial economic losses worldwide. To study the effects of
N. caninum
infection on the placenta, a pregnant mouse model for
N. caninum
infection was established. The litter size (8.6 ± 1.5) and the number of live pups (6.4 ± 1.8) of infected dams were significantly lower compared with those of non-infected dams. Trophoblast cell shrinkage and a large number of apoptosomes were detected in the placentas of the infected group. The parasite load in the placental tissue was significantly higher with time after infection. Likewise, apoptosis of placental trophoblast cells significantly increased with time after infection. Among the 66 apoptotic genes detected in this study, eight genes, including
Bcl
-
2
, were significantly differentially expressed by about > tenfold in infected and uninfected mice. The expression of BAX and tumor necrosis factor-alpha (TNF-α) was upregulated in the placental cells of the infected mice, whereas the expression of BCL-2 was downregulated. Enzyme-linked immunosorbent assays (ELISAs) showed that apoptotic protease caspase-3 level was significantly increased in placental cell suspension, and insulin-like growth factor (IGF)-2 level was significantly reduced. Acetylcholine (ACH) and placental prolactin (PL) levels were initially decreased but eventually increased. In summary, infection of mice with
N. caninum
caused apoptotic damage to the placental tissues, cells, and genes and affected the normal physiological functions of placenta, which may largely explain the adverse pregnancy outcomes caused by
N. caninum
infection in mice.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-022-07771-6</identifier><identifier>PMID: 36596902</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Apoptosis ; Atrophy ; Bcl-2 protein ; Biomedical and Life Sciences ; Biomedicine ; Caspase-3 ; Cattle ; Cattle Diseases - parasitology ; Coccidiosis - veterinary ; Dogs ; Female ; Immunology ; Infections ; Insulin ; Insulin-like growth factors ; Medical Microbiology ; Mice ; Mice, Inbred BALB C ; Microbiology ; Neospora - genetics ; Neosporosis ; Placenta ; Placenta - parasitology ; Pregnancy ; Prolactin ; Proto-Oncogene Proteins c-bcl-2 ; Sheep ; Trophoblasts ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α</subject><ispartof>Parasitology research (1987), 2023-03, Vol.122 (3), p.781-788</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-292f7702c35480a4ef8161bc4de6c0d27034f6fd31269f2f8e6f877ec910db53</citedby><cites>FETCH-LOGICAL-c375t-292f7702c35480a4ef8161bc4de6c0d27034f6fd31269f2f8e6f877ec910db53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-022-07771-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-022-07771-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36596902$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tang, Zeyu</creatorcontrib><creatorcontrib>Li, Hang</creatorcontrib><creatorcontrib>Xie, Suzhu</creatorcontrib><creatorcontrib>Zhao, Shaowei</creatorcontrib><creatorcontrib>Zhang, Shuang</creatorcontrib><creatorcontrib>Wang, Hao</creatorcontrib><creatorcontrib>Li, Nanli</creatorcontrib><creatorcontrib>Zhang, Xuancheng</creatorcontrib><creatorcontrib>Zhao, Fanglin</creatorcontrib><creatorcontrib>Jia, Lijun</creatorcontrib><title>A preliminary study on placental damage associated to experimental neosporosis in BALB/c mice</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>Neospora caninum
is a protozoan parasite which can infect a range of animals, including dogs, cattle, and sheep. Bovine neosporosis, which mainly causes abortion in cattle, results in substantial economic losses worldwide. To study the effects of
N. caninum
infection on the placenta, a pregnant mouse model for
N. caninum
infection was established. The litter size (8.6 ± 1.5) and the number of live pups (6.4 ± 1.8) of infected dams were significantly lower compared with those of non-infected dams. Trophoblast cell shrinkage and a large number of apoptosomes were detected in the placentas of the infected group. The parasite load in the placental tissue was significantly higher with time after infection. Likewise, apoptosis of placental trophoblast cells significantly increased with time after infection. Among the 66 apoptotic genes detected in this study, eight genes, including
Bcl
-
2
, were significantly differentially expressed by about > tenfold in infected and uninfected mice. The expression of BAX and tumor necrosis factor-alpha (TNF-α) was upregulated in the placental cells of the infected mice, whereas the expression of BCL-2 was downregulated. Enzyme-linked immunosorbent assays (ELISAs) showed that apoptotic protease caspase-3 level was significantly increased in placental cell suspension, and insulin-like growth factor (IGF)-2 level was significantly reduced. Acetylcholine (ACH) and placental prolactin (PL) levels were initially decreased but eventually increased. In summary, infection of mice with
N. caninum
caused apoptotic damage to the placental tissues, cells, and genes and affected the normal physiological functions of placenta, which may largely explain the adverse pregnancy outcomes caused by
N. caninum
infection in mice.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Atrophy</subject><subject>Bcl-2 protein</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Caspase-3</subject><subject>Cattle</subject><subject>Cattle Diseases - parasitology</subject><subject>Coccidiosis - veterinary</subject><subject>Dogs</subject><subject>Female</subject><subject>Immunology</subject><subject>Infections</subject><subject>Insulin</subject><subject>Insulin-like growth factors</subject><subject>Medical Microbiology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Neospora - genetics</subject><subject>Neosporosis</subject><subject>Placenta</subject><subject>Placenta - parasitology</subject><subject>Pregnancy</subject><subject>Prolactin</subject><subject>Proto-Oncogene Proteins c-bcl-2</subject><subject>Sheep</subject><subject>Trophoblasts</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis factor-α</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMoWqt_wIMEvHhZO0l2k91jW_yCgpdeJaTZSVnZLze7YP-9qVsVPHjKQJ55Z-Yh5IrBHQNQMw8QCxkB5xEopVgkj8iExYJHLEuSYzKBLNTAmDgj596_ATAl4_iUnAmZZDIDPiGvc9p2WBZVUZtuR30_5Dva1LQtjcW6NyXNTWW2SI33jS1MjzntG4ofLXZFNRI1Nr5tusYXnhY1XcxXi5mlVWHxgpw4U3q8PLxTsn64Xy-fotXL4_NyvoqsUEkf8Yw7pYBbkcQpmBhdyiTb2DhHaSHnCkTspMsF4zJz3KUoXaoU2oxBvknElNyOsW3XvA_oe10V3mJZmrDa4DVXElKmOLCA3vxB35qhq8NygUpFmqUA-0A-UjZc5Tt0ug3XBkGagd6716N7HdzrL_dahqbrQ_SwqTD_afmWHQAxAj581Vvsfmf_E_sJYDiOOw</recordid><startdate>20230301</startdate><enddate>20230301</enddate><creator>Tang, Zeyu</creator><creator>Li, Hang</creator><creator>Xie, Suzhu</creator><creator>Zhao, Shaowei</creator><creator>Zhang, Shuang</creator><creator>Wang, Hao</creator><creator>Li, Nanli</creator><creator>Zhang, Xuancheng</creator><creator>Zhao, Fanglin</creator><creator>Jia, Lijun</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20230301</creationdate><title>A preliminary study on placental damage associated to experimental neosporosis in BALB/c mice</title><author>Tang, Zeyu ; Li, Hang ; Xie, Suzhu ; Zhao, Shaowei ; Zhang, Shuang ; Wang, Hao ; Li, Nanli ; Zhang, Xuancheng ; Zhao, Fanglin ; Jia, Lijun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-292f7702c35480a4ef8161bc4de6c0d27034f6fd31269f2f8e6f877ec910db53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Atrophy</topic><topic>Bcl-2 protein</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Caspase-3</topic><topic>Cattle</topic><topic>Cattle Diseases - parasitology</topic><topic>Coccidiosis - veterinary</topic><topic>Dogs</topic><topic>Female</topic><topic>Immunology</topic><topic>Infections</topic><topic>Insulin</topic><topic>Insulin-like growth factors</topic><topic>Medical Microbiology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Neospora - genetics</topic><topic>Neosporosis</topic><topic>Placenta</topic><topic>Placenta - parasitology</topic><topic>Pregnancy</topic><topic>Prolactin</topic><topic>Proto-Oncogene Proteins c-bcl-2</topic><topic>Sheep</topic><topic>Trophoblasts</topic><topic>Tumor necrosis factor-TNF</topic><topic>Tumor necrosis factor-α</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tang, Zeyu</creatorcontrib><creatorcontrib>Li, Hang</creatorcontrib><creatorcontrib>Xie, Suzhu</creatorcontrib><creatorcontrib>Zhao, Shaowei</creatorcontrib><creatorcontrib>Zhang, Shuang</creatorcontrib><creatorcontrib>Wang, Hao</creatorcontrib><creatorcontrib>Li, Nanli</creatorcontrib><creatorcontrib>Zhang, Xuancheng</creatorcontrib><creatorcontrib>Zhao, Fanglin</creatorcontrib><creatorcontrib>Jia, Lijun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tang, Zeyu</au><au>Li, Hang</au><au>Xie, Suzhu</au><au>Zhao, Shaowei</au><au>Zhang, Shuang</au><au>Wang, Hao</au><au>Li, Nanli</au><au>Zhang, Xuancheng</au><au>Zhao, Fanglin</au><au>Jia, Lijun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A preliminary study on placental damage associated to experimental neosporosis in BALB/c mice</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2023-03-01</date><risdate>2023</risdate><volume>122</volume><issue>3</issue><spage>781</spage><epage>788</epage><pages>781-788</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><abstract>Neospora caninum
is a protozoan parasite which can infect a range of animals, including dogs, cattle, and sheep. Bovine neosporosis, which mainly causes abortion in cattle, results in substantial economic losses worldwide. To study the effects of
N. caninum
infection on the placenta, a pregnant mouse model for
N. caninum
infection was established. The litter size (8.6 ± 1.5) and the number of live pups (6.4 ± 1.8) of infected dams were significantly lower compared with those of non-infected dams. Trophoblast cell shrinkage and a large number of apoptosomes were detected in the placentas of the infected group. The parasite load in the placental tissue was significantly higher with time after infection. Likewise, apoptosis of placental trophoblast cells significantly increased with time after infection. Among the 66 apoptotic genes detected in this study, eight genes, including
Bcl
-
2
, were significantly differentially expressed by about > tenfold in infected and uninfected mice. The expression of BAX and tumor necrosis factor-alpha (TNF-α) was upregulated in the placental cells of the infected mice, whereas the expression of BCL-2 was downregulated. Enzyme-linked immunosorbent assays (ELISAs) showed that apoptotic protease caspase-3 level was significantly increased in placental cell suspension, and insulin-like growth factor (IGF)-2 level was significantly reduced. Acetylcholine (ACH) and placental prolactin (PL) levels were initially decreased but eventually increased. In summary, infection of mice with
N. caninum
caused apoptotic damage to the placental tissues, cells, and genes and affected the normal physiological functions of placenta, which may largely explain the adverse pregnancy outcomes caused by
N. caninum
infection in mice.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>36596902</pmid><doi>10.1007/s00436-022-07771-6</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0113 |
| ispartof | Parasitology research (1987), 2023-03, Vol.122 (3), p.781-788 |
| issn | 0932-0113 1432-1955 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2760817201 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Apoptosis Atrophy Bcl-2 protein Biomedical and Life Sciences Biomedicine Caspase-3 Cattle Cattle Diseases - parasitology Coccidiosis - veterinary Dogs Female Immunology Infections Insulin Insulin-like growth factors Medical Microbiology Mice Mice, Inbred BALB C Microbiology Neospora - genetics Neosporosis Placenta Placenta - parasitology Pregnancy Prolactin Proto-Oncogene Proteins c-bcl-2 Sheep Trophoblasts Tumor necrosis factor-TNF Tumor necrosis factor-α |
| title | A preliminary study on placental damage associated to experimental neosporosis in BALB/c mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T00%3A30%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20preliminary%20study%20on%20placental%20damage%20associated%20to%20experimental%20neosporosis%20in%20BALB/c%20mice&rft.jtitle=Parasitology%20research%20(1987)&rft.au=Tang,%20Zeyu&rft.date=2023-03-01&rft.volume=122&rft.issue=3&rft.spage=781&rft.epage=788&rft.pages=781-788&rft.issn=0932-0113&rft.eissn=1432-1955&rft_id=info:doi/10.1007/s00436-022-07771-6&rft_dat=%3Cproquest_cross%3E2760817201%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2783898005&rft_id=info:pmid/36596902&rfr_iscdi=true |