Composition characterization and biological activity study of Achillea vermicularis extract and extract‐loaded nanoparticles
Antiproliferative activity of Achillea vermicularis extracts was calculated on glial (C6) and keratinocyte (HaCaT) cell lines using XTT assay. It was observed that all extracts of A. vermicularis at the determined concentration were not cytotoxic in HaCaT cell lines. The nanoparticles (NPs) of the e...
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| Veröffentlicht in: | Biotechnology and applied biochemistry 2023-06, Vol.70 (3), p.1270-1278 |
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| creator | Doğan, Murat Koçyiğit, Ümit M. Çetin, Feyza Nur Yılmaz, Beyza Nur Taşkın, Turgut |
| description | Antiproliferative activity of Achillea vermicularis extracts was calculated on glial (C6) and keratinocyte (HaCaT) cell lines using XTT assay. It was observed that all extracts of A. vermicularis at the determined concentration were not cytotoxic in HaCaT cell lines. The nanoparticles (NPs) of the extract with the best cytotoxic activity was prepared, and necessary characterization studies were performed. Results showed that NP containing the extract has a lower IC50 value and more cytotoxic activity in C6 cells compared to the only extract. Furthermore, the antiepileptic potentials of these substances were explored in this study. The effect of A. vermicularis extracts on the enzyme activities of carbonic anhydrase I and II isoenzymes (hCA I and hCA II) was measured using spectrophotometry to achieve this goal. A. vermicularis extracts demonstrated high inhibitory activities compared to standard inhibitor (acetazolamide, AAZ), with IC50 values in the range of 5.04–10.8 μg/ml for hCA I, and 5.40–9.22 μg/ml for hCA II. High‐performance liquid chromatography diode array detector (HPLC‐DAD) was used in this investigation to assess the main chemicals found in the extract and NPs. The results showed that the ethanol extract (157.636 μg/mg extract) and NPs (4.631 μg/mg extract) had a significant amount of the 8‐hydroxy salvigenin component. |
| doi_str_mv | 10.1002/bab.2437 |
| format | Article |
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It was observed that all extracts of A. vermicularis at the determined concentration were not cytotoxic in HaCaT cell lines. The nanoparticles (NPs) of the extract with the best cytotoxic activity was prepared, and necessary characterization studies were performed. Results showed that NP containing the extract has a lower IC50 value and more cytotoxic activity in C6 cells compared to the only extract. Furthermore, the antiepileptic potentials of these substances were explored in this study. The effect of A. vermicularis extracts on the enzyme activities of carbonic anhydrase I and II isoenzymes (hCA I and hCA II) was measured using spectrophotometry to achieve this goal. A. vermicularis extracts demonstrated high inhibitory activities compared to standard inhibitor (acetazolamide, AAZ), with IC50 values in the range of 5.04–10.8 μg/ml for hCA I, and 5.40–9.22 μg/ml for hCA II. High‐performance liquid chromatography diode array detector (HPLC‐DAD) was used in this investigation to assess the main chemicals found in the extract and NPs. The results showed that the ethanol extract (157.636 μg/mg extract) and NPs (4.631 μg/mg extract) had a significant amount of the 8‐hydroxy salvigenin component.</description><identifier>ISSN: 0885-4513</identifier><identifier>EISSN: 1470-8744</identifier><identifier>DOI: 10.1002/bab.2437</identifier><identifier>PMID: 36594704</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Acetazolamide ; Achillea vermicularis ; Biological activity ; Carbonic anhydrase ; Carbonic anhydrase I ; Carbonic anhydrase II ; Cell lines ; cytotoxic activity ; Cytotoxicity ; encapsulation efficiency ; Enzymatic activity ; enzyme inhibition ; Ethanol ; High performance liquid chromatography ; Isoenzymes ; Liquid chromatography ; Nanoparticles ; Spectrophotometry</subject><ispartof>Biotechnology and applied biochemistry, 2023-06, Vol.70 (3), p.1270-1278</ispartof><rights>2023 International Union of Biochemistry and Molecular Biology, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3447-6aa6fb46a19f681b38a2e2963b7a946a1c74289284283b47af08134f5249ac623</cites><orcidid>0000-0003-2794-0177</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbab.2437$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbab.2437$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36594704$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Doğan, Murat</creatorcontrib><creatorcontrib>Koçyiğit, Ümit M.</creatorcontrib><creatorcontrib>Çetin, Feyza Nur</creatorcontrib><creatorcontrib>Yılmaz, Beyza Nur</creatorcontrib><creatorcontrib>Taşkın, Turgut</creatorcontrib><title>Composition characterization and biological activity study of Achillea vermicularis extract and extract‐loaded nanoparticles</title><title>Biotechnology and applied biochemistry</title><addtitle>Biotechnol Appl Biochem</addtitle><description>Antiproliferative activity of Achillea vermicularis extracts was calculated on glial (C6) and keratinocyte (HaCaT) cell lines using XTT assay. It was observed that all extracts of A. vermicularis at the determined concentration were not cytotoxic in HaCaT cell lines. The nanoparticles (NPs) of the extract with the best cytotoxic activity was prepared, and necessary characterization studies were performed. Results showed that NP containing the extract has a lower IC50 value and more cytotoxic activity in C6 cells compared to the only extract. Furthermore, the antiepileptic potentials of these substances were explored in this study. The effect of A. vermicularis extracts on the enzyme activities of carbonic anhydrase I and II isoenzymes (hCA I and hCA II) was measured using spectrophotometry to achieve this goal. A. vermicularis extracts demonstrated high inhibitory activities compared to standard inhibitor (acetazolamide, AAZ), with IC50 values in the range of 5.04–10.8 μg/ml for hCA I, and 5.40–9.22 μg/ml for hCA II. High‐performance liquid chromatography diode array detector (HPLC‐DAD) was used in this investigation to assess the main chemicals found in the extract and NPs. The results showed that the ethanol extract (157.636 μg/mg extract) and NPs (4.631 μg/mg extract) had a significant amount of the 8‐hydroxy salvigenin component.</description><subject>Acetazolamide</subject><subject>Achillea vermicularis</subject><subject>Biological activity</subject><subject>Carbonic anhydrase</subject><subject>Carbonic anhydrase I</subject><subject>Carbonic anhydrase II</subject><subject>Cell lines</subject><subject>cytotoxic activity</subject><subject>Cytotoxicity</subject><subject>encapsulation efficiency</subject><subject>Enzymatic activity</subject><subject>enzyme inhibition</subject><subject>Ethanol</subject><subject>High performance liquid chromatography</subject><subject>Isoenzymes</subject><subject>Liquid chromatography</subject><subject>Nanoparticles</subject><subject>Spectrophotometry</subject><issn>0885-4513</issn><issn>1470-8744</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp1kc9O3DAQh60K1N1CJZ6gssSFS8Cxndg5LisKSEi9lLM1cRzWKyfe2gmwHBCP0GfkSer901aq1IvHmvn8aeQfQic5Oc8JoRc11OeUM_EBTXMuSCYF5wdoSqQsMl7kbII-xbgkhEgh6Uc0YWVRJY5P0evcdysf7WB9j_UCAujBBPsC2wb0Da6td_7BanA4zeyjHdY4DmOzxr7FM72wzhnAjyZ0Vo8Ogo3YPA8bz_b5_v7-9tN5aEyDe-j9CsJgtTPxGB224KL5vK9H6P7r1ff5TXb37fp2PrvLNONcZCVA2da8hLxqS5nXTAI1tCpZLaDatLXgVFZUppPVXEBLZM54W1BegS4pO0JnO-8q-B-jiYPqbNTGOeiNH6OioiQFT99GEnr6D7r0Y-jTdopKKliiCPsr1MHHGEyrVsF2ENYqJ2qTiUqZqE0mCf2yF451Z5o_4O8QEpDtgCfrzPq_InU5u9wKfwFeh5dc</recordid><startdate>202306</startdate><enddate>202306</enddate><creator>Doğan, Murat</creator><creator>Koçyiğit, Ümit M.</creator><creator>Çetin, Feyza Nur</creator><creator>Yılmaz, Beyza Nur</creator><creator>Taşkın, Turgut</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TB</scope><scope>7TK</scope><scope>7U5</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>L7M</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2794-0177</orcidid></search><sort><creationdate>202306</creationdate><title>Composition characterization and biological activity study of Achillea vermicularis extract and extract‐loaded nanoparticles</title><author>Doğan, Murat ; Koçyiğit, Ümit M. ; Çetin, Feyza Nur ; Yılmaz, Beyza Nur ; Taşkın, Turgut</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3447-6aa6fb46a19f681b38a2e2963b7a946a1c74289284283b47af08134f5249ac623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Acetazolamide</topic><topic>Achillea vermicularis</topic><topic>Biological activity</topic><topic>Carbonic anhydrase</topic><topic>Carbonic anhydrase I</topic><topic>Carbonic anhydrase II</topic><topic>Cell lines</topic><topic>cytotoxic activity</topic><topic>Cytotoxicity</topic><topic>encapsulation efficiency</topic><topic>Enzymatic activity</topic><topic>enzyme inhibition</topic><topic>Ethanol</topic><topic>High performance liquid chromatography</topic><topic>Isoenzymes</topic><topic>Liquid chromatography</topic><topic>Nanoparticles</topic><topic>Spectrophotometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Doğan, Murat</creatorcontrib><creatorcontrib>Koçyiğit, Ümit M.</creatorcontrib><creatorcontrib>Çetin, Feyza Nur</creatorcontrib><creatorcontrib>Yılmaz, Beyza Nur</creatorcontrib><creatorcontrib>Taşkın, Turgut</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and applied biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Doğan, Murat</au><au>Koçyiğit, Ümit M.</au><au>Çetin, Feyza Nur</au><au>Yılmaz, Beyza Nur</au><au>Taşkın, Turgut</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Composition characterization and biological activity study of Achillea vermicularis extract and extract‐loaded nanoparticles</atitle><jtitle>Biotechnology and applied biochemistry</jtitle><addtitle>Biotechnol Appl Biochem</addtitle><date>2023-06</date><risdate>2023</risdate><volume>70</volume><issue>3</issue><spage>1270</spage><epage>1278</epage><pages>1270-1278</pages><issn>0885-4513</issn><eissn>1470-8744</eissn><abstract>Antiproliferative activity of Achillea vermicularis extracts was calculated on glial (C6) and keratinocyte (HaCaT) cell lines using XTT assay. It was observed that all extracts of A. vermicularis at the determined concentration were not cytotoxic in HaCaT cell lines. The nanoparticles (NPs) of the extract with the best cytotoxic activity was prepared, and necessary characterization studies were performed. Results showed that NP containing the extract has a lower IC50 value and more cytotoxic activity in C6 cells compared to the only extract. Furthermore, the antiepileptic potentials of these substances were explored in this study. The effect of A. vermicularis extracts on the enzyme activities of carbonic anhydrase I and II isoenzymes (hCA I and hCA II) was measured using spectrophotometry to achieve this goal. A. vermicularis extracts demonstrated high inhibitory activities compared to standard inhibitor (acetazolamide, AAZ), with IC50 values in the range of 5.04–10.8 μg/ml for hCA I, and 5.40–9.22 μg/ml for hCA II. High‐performance liquid chromatography diode array detector (HPLC‐DAD) was used in this investigation to assess the main chemicals found in the extract and NPs. The results showed that the ethanol extract (157.636 μg/mg extract) and NPs (4.631 μg/mg extract) had a significant amount of the 8‐hydroxy salvigenin component.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>36594704</pmid><doi>10.1002/bab.2437</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-2794-0177</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acetazolamide Achillea vermicularis Biological activity Carbonic anhydrase Carbonic anhydrase I Carbonic anhydrase II Cell lines cytotoxic activity Cytotoxicity encapsulation efficiency Enzymatic activity enzyme inhibition Ethanol High performance liquid chromatography Isoenzymes Liquid chromatography Nanoparticles Spectrophotometry |
| title | Composition characterization and biological activity study of Achillea vermicularis extract and extract‐loaded nanoparticles |
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