Effect of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia in vitro
The bone destruction in persistent apical periodontitis associated with infection and a periapical hypoxic microenvironment is not well known. Thus, we aimed to investigate the effects of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia. Mouse bone marrow-derived macrophages...
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| Veröffentlicht in: | Microbial pathogenesis 2023-02, Vol.175, p.105964-105964, Article 105964 |
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| creator | Zhou, Fengyi Li, Xin Chang, Xiaochi Geng, Zhihao Hao, Wenjing Deng, Jing Wong, Hai Ming Wang, Shuai |
| description | The bone destruction in persistent apical periodontitis associated with infection and a periapical hypoxic microenvironment is not well known. Thus, we aimed to investigate the effects of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia.
Mouse bone marrow-derived macrophages (BMMs) were isolated as osteoclast precursors and stimulated by heat-killed E. faecalis in an environment of cobalt-mimicked hypoxia environment. The cell proliferation and apoptosis were detected using CCK-8 and flow cytometry, respectively. Osteoclast differentiation was determined via tartrate-resistant acid phosphatase staining (TRAP) and immunofluorescence staining. The osteoclastogenic protein and gene expressions were measured by western blotting and real-time PCR.
Under cobalt-mimicked hypoxia, E. faecalis markedly inhibited the proliferation of the BMMs and significantly promoted the apoptosis of the BMMs. The differentiation of the BMMs into osteoclasts was enhanced in the presence of the E. faecalis under hypoxia, and the expression of Blimp, c-Fos, and NFATc1 was up-regulated, while the expression of RBP-J was inhibited.
E. faecalis markedly promotes osteoclast differentiation under cobalt-mimicked hypoxia in vitro.
•E. faecalis inhibited cell proliferation of osteoclast precursors under hypoxia.•E. faecalis promoted apoptosis of osteoclast precursors under hypoxia.•E. faecalis promoted osteoclast differentiation under hypoxia. |
| doi_str_mv | 10.1016/j.micpath.2022.105964 |
| format | Article |
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Mouse bone marrow-derived macrophages (BMMs) were isolated as osteoclast precursors and stimulated by heat-killed E. faecalis in an environment of cobalt-mimicked hypoxia environment. The cell proliferation and apoptosis were detected using CCK-8 and flow cytometry, respectively. Osteoclast differentiation was determined via tartrate-resistant acid phosphatase staining (TRAP) and immunofluorescence staining. The osteoclastogenic protein and gene expressions were measured by western blotting and real-time PCR.
Under cobalt-mimicked hypoxia, E. faecalis markedly inhibited the proliferation of the BMMs and significantly promoted the apoptosis of the BMMs. The differentiation of the BMMs into osteoclasts was enhanced in the presence of the E. faecalis under hypoxia, and the expression of Blimp, c-Fos, and NFATc1 was up-regulated, while the expression of RBP-J was inhibited.
E. faecalis markedly promotes osteoclast differentiation under cobalt-mimicked hypoxia in vitro.
•E. faecalis inhibited cell proliferation of osteoclast precursors under hypoxia.•E. faecalis promoted apoptosis of osteoclast precursors under hypoxia.•E. faecalis promoted osteoclast differentiation under hypoxia.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2022.105964</identifier><identifier>PMID: 36587925</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Apoptosis ; Bone marrow-derived macrophages ; Cell Differentiation ; Enterococcus faecalis ; Hypoxia ; Mice ; Osteoclasts ; Osteogenesis ; Proto-Oncogene Proteins c-fos</subject><ispartof>Microbial pathogenesis, 2023-02, Vol.175, p.105964-105964, Article 105964</ispartof><rights>2022 Elsevier Ltd</rights><rights>Copyright © 2022 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c313t-4fefe542f1a8e50f8b19b7e749f4324d56356d02f8ddfd19b49b599b6674cdc13</cites><orcidid>0000-0002-1760-6856</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micpath.2022.105964$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36587925$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Fengyi</creatorcontrib><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Chang, Xiaochi</creatorcontrib><creatorcontrib>Geng, Zhihao</creatorcontrib><creatorcontrib>Hao, Wenjing</creatorcontrib><creatorcontrib>Deng, Jing</creatorcontrib><creatorcontrib>Wong, Hai Ming</creatorcontrib><creatorcontrib>Wang, Shuai</creatorcontrib><title>Effect of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia in vitro</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>The bone destruction in persistent apical periodontitis associated with infection and a periapical hypoxic microenvironment is not well known. Thus, we aimed to investigate the effects of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia.
Mouse bone marrow-derived macrophages (BMMs) were isolated as osteoclast precursors and stimulated by heat-killed E. faecalis in an environment of cobalt-mimicked hypoxia environment. The cell proliferation and apoptosis were detected using CCK-8 and flow cytometry, respectively. Osteoclast differentiation was determined via tartrate-resistant acid phosphatase staining (TRAP) and immunofluorescence staining. The osteoclastogenic protein and gene expressions were measured by western blotting and real-time PCR.
Under cobalt-mimicked hypoxia, E. faecalis markedly inhibited the proliferation of the BMMs and significantly promoted the apoptosis of the BMMs. The differentiation of the BMMs into osteoclasts was enhanced in the presence of the E. faecalis under hypoxia, and the expression of Blimp, c-Fos, and NFATc1 was up-regulated, while the expression of RBP-J was inhibited.
E. faecalis markedly promotes osteoclast differentiation under cobalt-mimicked hypoxia in vitro.
•E. faecalis inhibited cell proliferation of osteoclast precursors under hypoxia.•E. faecalis promoted apoptosis of osteoclast precursors under hypoxia.•E. faecalis promoted osteoclast differentiation under hypoxia.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Bone marrow-derived macrophages</subject><subject>Cell Differentiation</subject><subject>Enterococcus faecalis</subject><subject>Hypoxia</subject><subject>Mice</subject><subject>Osteoclasts</subject><subject>Osteogenesis</subject><subject>Proto-Oncogene Proteins c-fos</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1O3DAUha0KVAboI1B5ySZT27GdeFUhNG2RkLopOyTLsa-LhySe2s4I3r5GM2Xb1ZXOPef-fAhdUbKmhMov2_UU7M6UpzUjjFVNKMk_oBUlSjaUkf4ErUjfs4YTSs7Qec5bQojirfqIzlop-k4xsUKPG-_BFhw93swFUrTR2iVjb8CaMWQcZxxzgWhHk0v8DTPkqi6zg4RtHMxYminUU57B4afXXXwJBocZ70NJ8RKdejNm-HSsF-jh2-bX7Y_m_uf3u9ub-8a2tC0N9-BBcOap6UEQ3w9UDR10XHneMu6EbIV0hPneOe9qj6tBKDVI2XHrLG0v0PVh7i7FPwvkoqeQLYyjmSEuWbNOEtox1opqFQerTTHnBF7vUphMetWU6DewequPYPUbWH0AW3OfjyuWYQL3nvpHshq-HgxQH90HSDrbALMFF1IFrF0M_1nxF8_Djao</recordid><startdate>202302</startdate><enddate>202302</enddate><creator>Zhou, Fengyi</creator><creator>Li, Xin</creator><creator>Chang, Xiaochi</creator><creator>Geng, Zhihao</creator><creator>Hao, Wenjing</creator><creator>Deng, Jing</creator><creator>Wong, Hai Ming</creator><creator>Wang, Shuai</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1760-6856</orcidid></search><sort><creationdate>202302</creationdate><title>Effect of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia in vitro</title><author>Zhou, Fengyi ; Li, Xin ; Chang, Xiaochi ; Geng, Zhihao ; Hao, Wenjing ; Deng, Jing ; Wong, Hai Ming ; Wang, Shuai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c313t-4fefe542f1a8e50f8b19b7e749f4324d56356d02f8ddfd19b49b599b6674cdc13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Bone marrow-derived macrophages</topic><topic>Cell Differentiation</topic><topic>Enterococcus faecalis</topic><topic>Hypoxia</topic><topic>Mice</topic><topic>Osteoclasts</topic><topic>Osteogenesis</topic><topic>Proto-Oncogene Proteins c-fos</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Fengyi</creatorcontrib><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Chang, Xiaochi</creatorcontrib><creatorcontrib>Geng, Zhihao</creatorcontrib><creatorcontrib>Hao, Wenjing</creatorcontrib><creatorcontrib>Deng, Jing</creatorcontrib><creatorcontrib>Wong, Hai Ming</creatorcontrib><creatorcontrib>Wang, Shuai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Fengyi</au><au>Li, Xin</au><au>Chang, Xiaochi</au><au>Geng, Zhihao</au><au>Hao, Wenjing</au><au>Deng, Jing</au><au>Wong, Hai Ming</au><au>Wang, Shuai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia in vitro</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2023-02</date><risdate>2023</risdate><volume>175</volume><spage>105964</spage><epage>105964</epage><pages>105964-105964</pages><artnum>105964</artnum><issn>0882-4010</issn><eissn>1096-1208</eissn><abstract>The bone destruction in persistent apical periodontitis associated with infection and a periapical hypoxic microenvironment is not well known. Thus, we aimed to investigate the effects of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia.
Mouse bone marrow-derived macrophages (BMMs) were isolated as osteoclast precursors and stimulated by heat-killed E. faecalis in an environment of cobalt-mimicked hypoxia environment. The cell proliferation and apoptosis were detected using CCK-8 and flow cytometry, respectively. Osteoclast differentiation was determined via tartrate-resistant acid phosphatase staining (TRAP) and immunofluorescence staining. The osteoclastogenic protein and gene expressions were measured by western blotting and real-time PCR.
Under cobalt-mimicked hypoxia, E. faecalis markedly inhibited the proliferation of the BMMs and significantly promoted the apoptosis of the BMMs. The differentiation of the BMMs into osteoclasts was enhanced in the presence of the E. faecalis under hypoxia, and the expression of Blimp, c-Fos, and NFATc1 was up-regulated, while the expression of RBP-J was inhibited.
E. faecalis markedly promotes osteoclast differentiation under cobalt-mimicked hypoxia in vitro.
•E. faecalis inhibited cell proliferation of osteoclast precursors under hypoxia.•E. faecalis promoted apoptosis of osteoclast precursors under hypoxia.•E. faecalis promoted osteoclast differentiation under hypoxia.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>36587925</pmid><doi>10.1016/j.micpath.2022.105964</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-1760-6856</orcidid></addata></record> |
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| subjects | Animals Apoptosis Bone marrow-derived macrophages Cell Differentiation Enterococcus faecalis Hypoxia Mice Osteoclasts Osteogenesis Proto-Oncogene Proteins c-fos |
| title | Effect of Enterococcus faecalis on osteoclastogenesis under cobalt-mimicked hypoxia in vitro |
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