A sandwich-type electrochemical immunosensor based on spherical nucleic acids-templated Ag nanoclusters for ultrasensitive detection of tumor biomarker
The accurate determination of tumor biomarkers in blood is of vital significance in the diagnosis and therapy of tumor disease. In this research, an innovative sandwich-type electrochemical immunosensor is designed for the ultrasensitive determination of tumor biomarker AFP using spherical nucleic a...
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| Veröffentlicht in: | Biosensors & bioelectronics 2023-03, Vol.223, p.115029-115029, Article 115029 |
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| container_title | Biosensors & bioelectronics |
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| creator | Chen, Huinan Li, Yuanyuan Song, Yuchen Liu, Fujing Deng, Dongmei Zhu, Xiaoli He, Haibo Yan, Xiaoxia Luo, Liqiang |
| description | The accurate determination of tumor biomarkers in blood is of vital significance in the diagnosis and therapy of tumor disease. In this research, an innovative sandwich-type electrochemical immunosensor is designed for the ultrasensitive determination of tumor biomarker AFP using spherical nucleic acids-templated silver nanoclusters (AgNCs) sensing platform. For this purpose, on one hand, DNA functionalized gold nanoparticles (AuNPs@DNA) is selected not only as the cross-linker to immobilize the primary antibody (anti-AFP antibody 1, Ab1) to obtain AuNPs@DNA-Ab1, but also as the template for synthesizing AgNCs on AuNPs to form AuNPs@DNA-AgNCs. On the other hand, p-sulfonated calix[4]arene (pSC4) modified Au is chosen to immobilize the secondary antibody (anti-AFP antibody 2, Ab2) through host-guest recognition between Ab2 and pSC4. When AFP is encountered, the immunoreaction signal can be significantly amplified by the electrochemical reduction of AgNCs. Under optimal circumstances, the sandwich-type electrochemical immunosensor exhibits broad limit of linearity from 0.001 to 100 ng mL−1 (R2 = 0.997) and low detection limit of 7.74 fg mL−1 (S/N = 3). The immunosensor possesses excellent repeatability and selectivity, offering a novel method for sensitive clinical diagnosis of tumor markers in human hepatocellular carcinoma.
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| doi_str_mv | 10.1016/j.bios.2022.115029 |
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[Display omitted]</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2022.115029</identifier><identifier>PMID: 36580814</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Ag nanoclusters ; Antibodies ; Biomarkers, Tumor ; Biosensing Techniques - methods ; Electrochemical Techniques - methods ; Gold ; Graphite ; Humans ; Immunoassay - methods ; Immunosensor ; Limit of Detection ; Metal Nanoparticles ; Nucleic Acids ; Signal amplifying ; Spherical nucleic acids ; Tumor biomarker</subject><ispartof>Biosensors & bioelectronics, 2023-03, Vol.223, p.115029-115029, Article 115029</ispartof><rights>2022 Elsevier B.V.</rights><rights>Copyright © 2022 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-ddf9fe41a3ed39e0f86309ca8e6e825207adc3e39108f63701f72771ef07e2233</citedby><cites>FETCH-LOGICAL-c356t-ddf9fe41a3ed39e0f86309ca8e6e825207adc3e39108f63701f72771ef07e2233</cites><orcidid>0000-0002-5551-3861 ; 0000-0001-6212-4138</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2022.115029$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36580814$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Huinan</creatorcontrib><creatorcontrib>Li, Yuanyuan</creatorcontrib><creatorcontrib>Song, Yuchen</creatorcontrib><creatorcontrib>Liu, Fujing</creatorcontrib><creatorcontrib>Deng, Dongmei</creatorcontrib><creatorcontrib>Zhu, Xiaoli</creatorcontrib><creatorcontrib>He, Haibo</creatorcontrib><creatorcontrib>Yan, Xiaoxia</creatorcontrib><creatorcontrib>Luo, Liqiang</creatorcontrib><title>A sandwich-type electrochemical immunosensor based on spherical nucleic acids-templated Ag nanoclusters for ultrasensitive detection of tumor biomarker</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>The accurate determination of tumor biomarkers in blood is of vital significance in the diagnosis and therapy of tumor disease. In this research, an innovative sandwich-type electrochemical immunosensor is designed for the ultrasensitive determination of tumor biomarker AFP using spherical nucleic acids-templated silver nanoclusters (AgNCs) sensing platform. For this purpose, on one hand, DNA functionalized gold nanoparticles (AuNPs@DNA) is selected not only as the cross-linker to immobilize the primary antibody (anti-AFP antibody 1, Ab1) to obtain AuNPs@DNA-Ab1, but also as the template for synthesizing AgNCs on AuNPs to form AuNPs@DNA-AgNCs. On the other hand, p-sulfonated calix[4]arene (pSC4) modified Au is chosen to immobilize the secondary antibody (anti-AFP antibody 2, Ab2) through host-guest recognition between Ab2 and pSC4. When AFP is encountered, the immunoreaction signal can be significantly amplified by the electrochemical reduction of AgNCs. Under optimal circumstances, the sandwich-type electrochemical immunosensor exhibits broad limit of linearity from 0.001 to 100 ng mL−1 (R2 = 0.997) and low detection limit of 7.74 fg mL−1 (S/N = 3). The immunosensor possesses excellent repeatability and selectivity, offering a novel method for sensitive clinical diagnosis of tumor markers in human hepatocellular carcinoma.
[Display omitted]</description><subject>Ag nanoclusters</subject><subject>Antibodies</subject><subject>Biomarkers, Tumor</subject><subject>Biosensing Techniques - methods</subject><subject>Electrochemical Techniques - methods</subject><subject>Gold</subject><subject>Graphite</subject><subject>Humans</subject><subject>Immunoassay - methods</subject><subject>Immunosensor</subject><subject>Limit of Detection</subject><subject>Metal Nanoparticles</subject><subject>Nucleic Acids</subject><subject>Signal amplifying</subject><subject>Spherical nucleic acids</subject><subject>Tumor biomarker</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuOFCEUholx4rQz8wIuDEs31XJpqCJx05l4SyZx46wJDQebtgpKoMbMk_i6Uvbo0hULvv8_HD6EXlGypYTKt6ftIaSyZYSxLaWCMPUMbejQ827HuHiONkQJ2Qkp-SV6WcqJENJTRV6gSy7FQAa626Bfe1xMdD-DPXb1cQYMI9iakz3CFKwZcZimJaYCsaSMD6aAwyniMh8h_7mPix0hWGxscKWrMM2jqQ3af8PRxGTHpVTIBfsWX8aazVoVangA7KC2WaHVJY_rMq0DQppM_g75Gl14Mxa4eTqv0P2H919vP3V3Xz5-vt3fdZYLWTvnvPKwo4aD4wqIHyQnypoBJAxMMNIbZzlwRcngJe8J9T3rewqe9MAY51fozbl3zunHAqXqKRQL42gipKVo1gulhCKSNZSdUZtTKRm8nnNor33UlOhViD7pVYheheizkBZ6_dS_HCZw_yJ_DTTg3RmAtuVDgKyLDRAtuJDb72iXwv_6fwPBMKCB</recordid><startdate>20230301</startdate><enddate>20230301</enddate><creator>Chen, Huinan</creator><creator>Li, Yuanyuan</creator><creator>Song, Yuchen</creator><creator>Liu, Fujing</creator><creator>Deng, Dongmei</creator><creator>Zhu, Xiaoli</creator><creator>He, Haibo</creator><creator>Yan, Xiaoxia</creator><creator>Luo, Liqiang</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5551-3861</orcidid><orcidid>https://orcid.org/0000-0001-6212-4138</orcidid></search><sort><creationdate>20230301</creationdate><title>A sandwich-type electrochemical immunosensor based on spherical nucleic acids-templated Ag nanoclusters for ultrasensitive detection of tumor biomarker</title><author>Chen, Huinan ; Li, Yuanyuan ; Song, Yuchen ; Liu, Fujing ; Deng, Dongmei ; Zhu, Xiaoli ; He, Haibo ; Yan, Xiaoxia ; Luo, Liqiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-ddf9fe41a3ed39e0f86309ca8e6e825207adc3e39108f63701f72771ef07e2233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Ag nanoclusters</topic><topic>Antibodies</topic><topic>Biomarkers, Tumor</topic><topic>Biosensing Techniques - methods</topic><topic>Electrochemical Techniques - methods</topic><topic>Gold</topic><topic>Graphite</topic><topic>Humans</topic><topic>Immunoassay - methods</topic><topic>Immunosensor</topic><topic>Limit of Detection</topic><topic>Metal Nanoparticles</topic><topic>Nucleic Acids</topic><topic>Signal amplifying</topic><topic>Spherical nucleic acids</topic><topic>Tumor biomarker</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Huinan</creatorcontrib><creatorcontrib>Li, Yuanyuan</creatorcontrib><creatorcontrib>Song, Yuchen</creatorcontrib><creatorcontrib>Liu, Fujing</creatorcontrib><creatorcontrib>Deng, Dongmei</creatorcontrib><creatorcontrib>Zhu, Xiaoli</creatorcontrib><creatorcontrib>He, Haibo</creatorcontrib><creatorcontrib>Yan, Xiaoxia</creatorcontrib><creatorcontrib>Luo, Liqiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Huinan</au><au>Li, Yuanyuan</au><au>Song, Yuchen</au><au>Liu, Fujing</au><au>Deng, Dongmei</au><au>Zhu, Xiaoli</au><au>He, Haibo</au><au>Yan, Xiaoxia</au><au>Luo, Liqiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A sandwich-type electrochemical immunosensor based on spherical nucleic acids-templated Ag nanoclusters for ultrasensitive detection of tumor biomarker</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2023-03-01</date><risdate>2023</risdate><volume>223</volume><spage>115029</spage><epage>115029</epage><pages>115029-115029</pages><artnum>115029</artnum><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>The accurate determination of tumor biomarkers in blood is of vital significance in the diagnosis and therapy of tumor disease. In this research, an innovative sandwich-type electrochemical immunosensor is designed for the ultrasensitive determination of tumor biomarker AFP using spherical nucleic acids-templated silver nanoclusters (AgNCs) sensing platform. For this purpose, on one hand, DNA functionalized gold nanoparticles (AuNPs@DNA) is selected not only as the cross-linker to immobilize the primary antibody (anti-AFP antibody 1, Ab1) to obtain AuNPs@DNA-Ab1, but also as the template for synthesizing AgNCs on AuNPs to form AuNPs@DNA-AgNCs. On the other hand, p-sulfonated calix[4]arene (pSC4) modified Au is chosen to immobilize the secondary antibody (anti-AFP antibody 2, Ab2) through host-guest recognition between Ab2 and pSC4. When AFP is encountered, the immunoreaction signal can be significantly amplified by the electrochemical reduction of AgNCs. Under optimal circumstances, the sandwich-type electrochemical immunosensor exhibits broad limit of linearity from 0.001 to 100 ng mL−1 (R2 = 0.997) and low detection limit of 7.74 fg mL−1 (S/N = 3). The immunosensor possesses excellent repeatability and selectivity, offering a novel method for sensitive clinical diagnosis of tumor markers in human hepatocellular carcinoma.
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| subjects | Ag nanoclusters Antibodies Biomarkers, Tumor Biosensing Techniques - methods Electrochemical Techniques - methods Gold Graphite Humans Immunoassay - methods Immunosensor Limit of Detection Metal Nanoparticles Nucleic Acids Signal amplifying Spherical nucleic acids Tumor biomarker |
| title | A sandwich-type electrochemical immunosensor based on spherical nucleic acids-templated Ag nanoclusters for ultrasensitive detection of tumor biomarker |
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