Protective effects of different doses of MitoQ separately and combined with trehalose on oxidative stress and sperm function of cryopreserved Markhoz goat semen
The mitochondria-targeted antioxidant MitoQ has been regarded as an effective antioxidant agent against cryo-induced oxidative cellular damage. This study aimed to evaluate the use of different doses of MitoQ combined with trehalose to minimize mitochondrial impairment and oxidative stress during sp...
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| Veröffentlicht in: | Cryobiology 2023-03, Vol.110, p.36-43 |
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| creator | Rezaei, Ako Bahmani, Hamid Reza Mafakheri, Shiva Farshad, Abbas Nazari, Parisa Masoudi, Reza |
| description | The mitochondria-targeted antioxidant MitoQ has been regarded as an effective antioxidant agent against cryo-induced oxidative cellular damage. This study aimed to evaluate the use of different doses of MitoQ combined with trehalose to minimize mitochondrial impairment and oxidative stress during sperm cryopreservation of Markhoz goat. For this, semen samples (n = 50) were collected by electroejaculation every 5 days from 5 bucks in 10 replicates. On each collection day, 5 ejaculates (one ejaculate for each buck) were pooled and then diluted in eight different Tris-based extenders as follows: no additives (control), 20, 200, 2000 nM of MitoQ (MT20, MT200, MT 2000, respectively), 150 mM of trehalose (Tr), MT20+Tr, MT200+Tr, MT2000+Tr. The semen samples were frozen using a standard protocol, and sperm function and oxidative stress were evaluated after thawing. The semen extender supplemented with MT200+Tr had higher (P |
| doi_str_mv | 10.1016/j.cryobiol.2022.12.019 |
| format | Article |
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This study aimed to evaluate the use of different doses of MitoQ combined with trehalose to minimize mitochondrial impairment and oxidative stress during sperm cryopreservation of Markhoz goat. For this, semen samples (n = 50) were collected by electroejaculation every 5 days from 5 bucks in 10 replicates. On each collection day, 5 ejaculates (one ejaculate for each buck) were pooled and then diluted in eight different Tris-based extenders as follows: no additives (control), 20, 200, 2000 nM of MitoQ (MT20, MT200, MT 2000, respectively), 150 mM of trehalose (Tr), MT20+Tr, MT200+Tr, MT2000+Tr. The semen samples were frozen using a standard protocol, and sperm function and oxidative stress were evaluated after thawing. The semen extender supplemented with MT200+Tr had higher (P < 0.05) total and progressive motility, acrosome and membrane integrity, superoxide dismutase, glutathione peroxidase, total antioxidant capacity, and lower (P < 0.05) DNA fragmentation, malondialdehyde and intracellular hydrogen peroxide levels than the all other groups except MT200; meanwhile, MT200 was also improved (P < 0.05) in these parameters than in the control group. Furthermore, MT200 and MT200+Tr showed higher (P < 0.05) percentages of live cryopreserved sperm with high mitochondrial activity than other groups. However, abnormality percentage and catalase activity of frozen-thawed sperm were not affected by treatments (P > 0.05). To conclude, we have found that supplementation of 200 nM MitoQ alone or in combination with 150 mM trehalose to semen extender improved the quality of cryopreserved sperm in goats, which is associated with enhanced antioxidant enzymatic defense and mitochondrial activity and reduced DNA fragmentation.</description><identifier>ISSN: 0011-2240</identifier><identifier>EISSN: 1090-2392</identifier><identifier>DOI: 10.1016/j.cryobiol.2022.12.019</identifier><identifier>PMID: 36581061</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Antioxidants - pharmacology ; Cryopreservation - methods ; Goat ; Goats - metabolism ; Male ; Mitochondrial activity ; MitoQ ; Oxidative Stress ; Semen ; Semen Analysis - veterinary ; Semen cryopreservation ; Semen Preservation - methods ; Sperm Motility ; Spermatozoa ; Trehalose - pharmacology</subject><ispartof>Cryobiology, 2023-03, Vol.110, p.36-43</ispartof><rights>2022 Elsevier Inc.</rights><rights>Copyright © 2022 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-17105f184bf47f5e25fee69b8d25fc598a07adb3974004ed40d6f06cc17011053</citedby><cites>FETCH-LOGICAL-c368t-17105f184bf47f5e25fee69b8d25fc598a07adb3974004ed40d6f06cc17011053</cites><orcidid>0000-0002-1502-7797 ; 0000-0001-9467-4353 ; 0000-0001-7899-7654</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cryobiol.2022.12.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36581061$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rezaei, Ako</creatorcontrib><creatorcontrib>Bahmani, Hamid Reza</creatorcontrib><creatorcontrib>Mafakheri, Shiva</creatorcontrib><creatorcontrib>Farshad, Abbas</creatorcontrib><creatorcontrib>Nazari, Parisa</creatorcontrib><creatorcontrib>Masoudi, Reza</creatorcontrib><title>Protective effects of different doses of MitoQ separately and combined with trehalose on oxidative stress and sperm function of cryopreserved Markhoz goat semen</title><title>Cryobiology</title><addtitle>Cryobiology</addtitle><description>The mitochondria-targeted antioxidant MitoQ has been regarded as an effective antioxidant agent against cryo-induced oxidative cellular damage. This study aimed to evaluate the use of different doses of MitoQ combined with trehalose to minimize mitochondrial impairment and oxidative stress during sperm cryopreservation of Markhoz goat. For this, semen samples (n = 50) were collected by electroejaculation every 5 days from 5 bucks in 10 replicates. On each collection day, 5 ejaculates (one ejaculate for each buck) were pooled and then diluted in eight different Tris-based extenders as follows: no additives (control), 20, 200, 2000 nM of MitoQ (MT20, MT200, MT 2000, respectively), 150 mM of trehalose (Tr), MT20+Tr, MT200+Tr, MT2000+Tr. The semen samples were frozen using a standard protocol, and sperm function and oxidative stress were evaluated after thawing. The semen extender supplemented with MT200+Tr had higher (P < 0.05) total and progressive motility, acrosome and membrane integrity, superoxide dismutase, glutathione peroxidase, total antioxidant capacity, and lower (P < 0.05) DNA fragmentation, malondialdehyde and intracellular hydrogen peroxide levels than the all other groups except MT200; meanwhile, MT200 was also improved (P < 0.05) in these parameters than in the control group. Furthermore, MT200 and MT200+Tr showed higher (P < 0.05) percentages of live cryopreserved sperm with high mitochondrial activity than other groups. However, abnormality percentage and catalase activity of frozen-thawed sperm were not affected by treatments (P > 0.05). To conclude, we have found that supplementation of 200 nM MitoQ alone or in combination with 150 mM trehalose to semen extender improved the quality of cryopreserved sperm in goats, which is associated with enhanced antioxidant enzymatic defense and mitochondrial activity and reduced DNA fragmentation.</description><subject>Animals</subject><subject>Antioxidants - pharmacology</subject><subject>Cryopreservation - methods</subject><subject>Goat</subject><subject>Goats - metabolism</subject><subject>Male</subject><subject>Mitochondrial activity</subject><subject>MitoQ</subject><subject>Oxidative Stress</subject><subject>Semen</subject><subject>Semen Analysis - veterinary</subject><subject>Semen cryopreservation</subject><subject>Semen Preservation - methods</subject><subject>Sperm Motility</subject><subject>Spermatozoa</subject><subject>Trehalose - pharmacology</subject><issn>0011-2240</issn><issn>1090-2392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUctuEzEUtRCIhsIvVF6ymeHa8_QOVNEWqRVFgrXlsa-Jw8x4sJ1A-Bo-tU7SsmXlo3vPQ9eHkAsGJQPWvtuUOuz94PxYcuC8ZLwEJp6RFQMBBa8Ef05WAIwVnNdwRl7FuAGAtqvql-SsapueQctW5O998Al1cjukaG1GkXpLjcs44Jyo8RGPozuX_BcacVFBJRz3VM2Gaj8NbkZDf7m0pingWo1ZQP1M_W9n1NE35nmMR35cMEzUbueceOBYejhjyXsMu2xzp8KPtf9Dv3uVctaE82vywqox4pvH95x8u_r49fKmuP18_enyw22hq7ZPBesYNJb19WDrzjbIG4vYiqE3GelG9Ao6ZYZKdDVAjaYG01potWZd_iRoqnPy9uS7BP9zizHJyUWN46hm9NsoedcI0fSC80xtT1QdfIwBrVyCm1TYSwby0I7cyKd25KEdybjM7WThxWPGdpjQ_JM91ZEJ708EzJfuHAYZtcNZo3EhVyONd__LeACs8ag1</recordid><startdate>202303</startdate><enddate>202303</enddate><creator>Rezaei, Ako</creator><creator>Bahmani, Hamid Reza</creator><creator>Mafakheri, Shiva</creator><creator>Farshad, Abbas</creator><creator>Nazari, Parisa</creator><creator>Masoudi, Reza</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1502-7797</orcidid><orcidid>https://orcid.org/0000-0001-9467-4353</orcidid><orcidid>https://orcid.org/0000-0001-7899-7654</orcidid></search><sort><creationdate>202303</creationdate><title>Protective effects of different doses of MitoQ separately and combined with trehalose on oxidative stress and sperm function of cryopreserved Markhoz goat semen</title><author>Rezaei, Ako ; Bahmani, Hamid Reza ; Mafakheri, Shiva ; Farshad, Abbas ; Nazari, Parisa ; Masoudi, Reza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-17105f184bf47f5e25fee69b8d25fc598a07adb3974004ed40d6f06cc17011053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Antioxidants - pharmacology</topic><topic>Cryopreservation - methods</topic><topic>Goat</topic><topic>Goats - metabolism</topic><topic>Male</topic><topic>Mitochondrial activity</topic><topic>MitoQ</topic><topic>Oxidative Stress</topic><topic>Semen</topic><topic>Semen Analysis - veterinary</topic><topic>Semen cryopreservation</topic><topic>Semen Preservation - methods</topic><topic>Sperm Motility</topic><topic>Spermatozoa</topic><topic>Trehalose - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rezaei, Ako</creatorcontrib><creatorcontrib>Bahmani, Hamid Reza</creatorcontrib><creatorcontrib>Mafakheri, Shiva</creatorcontrib><creatorcontrib>Farshad, Abbas</creatorcontrib><creatorcontrib>Nazari, Parisa</creatorcontrib><creatorcontrib>Masoudi, Reza</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cryobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rezaei, Ako</au><au>Bahmani, Hamid Reza</au><au>Mafakheri, Shiva</au><au>Farshad, Abbas</au><au>Nazari, Parisa</au><au>Masoudi, Reza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective effects of different doses of MitoQ separately and combined with trehalose on oxidative stress and sperm function of cryopreserved Markhoz goat semen</atitle><jtitle>Cryobiology</jtitle><addtitle>Cryobiology</addtitle><date>2023-03</date><risdate>2023</risdate><volume>110</volume><spage>36</spage><epage>43</epage><pages>36-43</pages><issn>0011-2240</issn><eissn>1090-2392</eissn><abstract>The mitochondria-targeted antioxidant MitoQ has been regarded as an effective antioxidant agent against cryo-induced oxidative cellular damage. This study aimed to evaluate the use of different doses of MitoQ combined with trehalose to minimize mitochondrial impairment and oxidative stress during sperm cryopreservation of Markhoz goat. For this, semen samples (n = 50) were collected by electroejaculation every 5 days from 5 bucks in 10 replicates. On each collection day, 5 ejaculates (one ejaculate for each buck) were pooled and then diluted in eight different Tris-based extenders as follows: no additives (control), 20, 200, 2000 nM of MitoQ (MT20, MT200, MT 2000, respectively), 150 mM of trehalose (Tr), MT20+Tr, MT200+Tr, MT2000+Tr. The semen samples were frozen using a standard protocol, and sperm function and oxidative stress were evaluated after thawing. The semen extender supplemented with MT200+Tr had higher (P < 0.05) total and progressive motility, acrosome and membrane integrity, superoxide dismutase, glutathione peroxidase, total antioxidant capacity, and lower (P < 0.05) DNA fragmentation, malondialdehyde and intracellular hydrogen peroxide levels than the all other groups except MT200; meanwhile, MT200 was also improved (P < 0.05) in these parameters than in the control group. Furthermore, MT200 and MT200+Tr showed higher (P < 0.05) percentages of live cryopreserved sperm with high mitochondrial activity than other groups. However, abnormality percentage and catalase activity of frozen-thawed sperm were not affected by treatments (P > 0.05). To conclude, we have found that supplementation of 200 nM MitoQ alone or in combination with 150 mM trehalose to semen extender improved the quality of cryopreserved sperm in goats, which is associated with enhanced antioxidant enzymatic defense and mitochondrial activity and reduced DNA fragmentation.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>36581061</pmid><doi>10.1016/j.cryobiol.2022.12.019</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-1502-7797</orcidid><orcidid>https://orcid.org/0000-0001-9467-4353</orcidid><orcidid>https://orcid.org/0000-0001-7899-7654</orcidid></addata></record> |
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| ispartof | Cryobiology, 2023-03, Vol.110, p.36-43 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Antioxidants - pharmacology Cryopreservation - methods Goat Goats - metabolism Male Mitochondrial activity MitoQ Oxidative Stress Semen Semen Analysis - veterinary Semen cryopreservation Semen Preservation - methods Sperm Motility Spermatozoa Trehalose - pharmacology |
| title | Protective effects of different doses of MitoQ separately and combined with trehalose on oxidative stress and sperm function of cryopreserved Markhoz goat semen |
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