GC/MS and LC/MS Phytochemical Analysis of Vigna unguiculata L. Walp Pod
Vigna unguiculata (L. Walp) or Cowpea pod methanolic extracts phytochemical analysis, total phenolic content (TPC), and secondary metabolite profiling were determined using gas chromatography‐mass spectrometry (GC/MS) and liquid chromatography‐mass spectrometry (LC/MS) analysis. GC/MS analysis revea...
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| Veröffentlicht in: | Chemistry & biodiversity 2023-02, Vol.20 (2), p.e202200048-n/a |
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| creator | Mahavirsing Dinore, Jaysing Shivaji Patil, Harshal Farooqui, Samreen Pradhan, Vidya Farooqui, Mazahar |
| description | Vigna unguiculata (L. Walp) or Cowpea pod methanolic extracts phytochemical analysis, total phenolic content (TPC), and secondary metabolite profiling were determined using gas chromatography‐mass spectrometry (GC/MS) and liquid chromatography‐mass spectrometry (LC/MS) analysis. GC/MS analysis revealed twenty compounds in the extract, while LC/MS analysis identified twenty‐four compounds. GC/MS chromatogram analysis suggested the presence of opioid α‐N‐Normethadol a major constituent found in methanolic extract and fatty acid esters carotenoid is found second major constituent. LC/MS chromatogram and the mass spectral analysis demonstrated the presence of flavonoids, carotenoids, and alkaloids as major phytochemicals. We investigated the antibacterial, anti‐fungal, and anti‐oxidant activity of pod methanolic extract. The extract was found equally effective against E. coli, S. pyogenes, and P. aeruginosa with MIC 100 μg/mL similar to the standard Ampicillin (MIC 100 μg/mL). C. albicans were found to be most susceptible to Vign unguiculata pods methanolic extract with a MIC of 250 μg/mL. The pod extract showed significant DPPH scavenging activity (IC50=78.38±0.15) which suggests its antioxidant potential. |
| doi_str_mv | 10.1002/cbdv.202200048 |
| format | Article |
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Walp Pod</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Mahavirsing Dinore, Jaysing ; Shivaji Patil, Harshal ; Farooqui, Samreen ; Pradhan, Vidya ; Farooqui, Mazahar</creator><creatorcontrib>Mahavirsing Dinore, Jaysing ; Shivaji Patil, Harshal ; Farooqui, Samreen ; Pradhan, Vidya ; Farooqui, Mazahar</creatorcontrib><description>Vigna unguiculata (L. Walp) or Cowpea pod methanolic extracts phytochemical analysis, total phenolic content (TPC), and secondary metabolite profiling were determined using gas chromatography‐mass spectrometry (GC/MS) and liquid chromatography‐mass spectrometry (LC/MS) analysis. GC/MS analysis revealed twenty compounds in the extract, while LC/MS analysis identified twenty‐four compounds. GC/MS chromatogram analysis suggested the presence of opioid α‐N‐Normethadol a major constituent found in methanolic extract and fatty acid esters carotenoid is found second major constituent. LC/MS chromatogram and the mass spectral analysis demonstrated the presence of flavonoids, carotenoids, and alkaloids as major phytochemicals. We investigated the antibacterial, anti‐fungal, and anti‐oxidant activity of pod methanolic extract. The extract was found equally effective against E. coli, S. pyogenes, and P. aeruginosa with MIC 100 μg/mL similar to the standard Ampicillin (MIC 100 μg/mL). C. albicans were found to be most susceptible to Vign unguiculata pods methanolic extract with a MIC of 250 μg/mL. The pod extract showed significant DPPH scavenging activity (IC50=78.38±0.15) which suggests its antioxidant potential.</description><identifier>ISSN: 1612-1872</identifier><identifier>EISSN: 1612-1880</identifier><identifier>DOI: 10.1002/cbdv.202200048</identifier><identifier>PMID: 36576750</identifier><language>eng</language><publisher>Switzerland: Wiley Subscription Services, Inc</publisher><subject>Ampicillin ; antimicrobial activity ; Antimicrobial agents ; antioxidant activity ; Antioxidants - chemistry ; Carotenoids ; Chromatography ; Chromatography, Liquid ; Constituents ; cowpea ; Cowpeas ; E coli ; Escherichia coli ; Esters ; Fatty acids ; Flavonoids ; Gas chromatography ; Gas Chromatography-Mass Spectrometry ; GC/MS ; LC/MS ; Liquid chromatography ; Mass Spectrometry ; Mass spectroscopy ; Metabolites ; Methanol ; Minimum inhibitory concentration ; Oxidants ; Oxidizing agents ; Phenolic compounds ; Phenols ; Phytochemicals ; Phytochemicals - chemistry ; Plant Extracts - chemistry ; Scavenging ; Scientific imaging ; Spectral analysis ; Spectrum analysis ; Vigna - chemistry ; Vigna unguiculata</subject><ispartof>Chemistry & biodiversity, 2023-02, Vol.20 (2), p.e202200048-n/a</ispartof><rights>2022 Wiley‐VHCA AG, Zurich, Switzerland</rights><rights>2022 Wiley-VHCA AG, Zurich, Switzerland.</rights><rights>2023 Wiley‐VHCA AG, Zurich, Switzerland</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3038-11a42dffbf5cbb43aa5a0bc1454755051017f421cf1e8b0419253f1e5207fcad3</citedby><cites>FETCH-LOGICAL-c3038-11a42dffbf5cbb43aa5a0bc1454755051017f421cf1e8b0419253f1e5207fcad3</cites><orcidid>0000-0002-6388-8663 ; 0000-0002-0819-4984</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbdv.202200048$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbdv.202200048$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27907,27908,45557,45558</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36576750$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mahavirsing Dinore, Jaysing</creatorcontrib><creatorcontrib>Shivaji Patil, Harshal</creatorcontrib><creatorcontrib>Farooqui, Samreen</creatorcontrib><creatorcontrib>Pradhan, Vidya</creatorcontrib><creatorcontrib>Farooqui, Mazahar</creatorcontrib><title>GC/MS and LC/MS Phytochemical Analysis of Vigna unguiculata L. Walp Pod</title><title>Chemistry & biodiversity</title><addtitle>Chem Biodivers</addtitle><description>Vigna unguiculata (L. Walp) or Cowpea pod methanolic extracts phytochemical analysis, total phenolic content (TPC), and secondary metabolite profiling were determined using gas chromatography‐mass spectrometry (GC/MS) and liquid chromatography‐mass spectrometry (LC/MS) analysis. GC/MS analysis revealed twenty compounds in the extract, while LC/MS analysis identified twenty‐four compounds. GC/MS chromatogram analysis suggested the presence of opioid α‐N‐Normethadol a major constituent found in methanolic extract and fatty acid esters carotenoid is found second major constituent. LC/MS chromatogram and the mass spectral analysis demonstrated the presence of flavonoids, carotenoids, and alkaloids as major phytochemicals. We investigated the antibacterial, anti‐fungal, and anti‐oxidant activity of pod methanolic extract. The extract was found equally effective against E. coli, S. pyogenes, and P. aeruginosa with MIC 100 μg/mL similar to the standard Ampicillin (MIC 100 μg/mL). C. albicans were found to be most susceptible to Vign unguiculata pods methanolic extract with a MIC of 250 μg/mL. The pod extract showed significant DPPH scavenging activity (IC50=78.38±0.15) which suggests its antioxidant potential.</description><subject>Ampicillin</subject><subject>antimicrobial activity</subject><subject>Antimicrobial agents</subject><subject>antioxidant activity</subject><subject>Antioxidants - chemistry</subject><subject>Carotenoids</subject><subject>Chromatography</subject><subject>Chromatography, Liquid</subject><subject>Constituents</subject><subject>cowpea</subject><subject>Cowpeas</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Esters</subject><subject>Fatty acids</subject><subject>Flavonoids</subject><subject>Gas chromatography</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>GC/MS</subject><subject>LC/MS</subject><subject>Liquid chromatography</subject><subject>Mass Spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metabolites</subject><subject>Methanol</subject><subject>Minimum inhibitory concentration</subject><subject>Oxidants</subject><subject>Oxidizing agents</subject><subject>Phenolic compounds</subject><subject>Phenols</subject><subject>Phytochemicals</subject><subject>Phytochemicals - chemistry</subject><subject>Plant Extracts - chemistry</subject><subject>Scavenging</subject><subject>Scientific imaging</subject><subject>Spectral analysis</subject><subject>Spectrum analysis</subject><subject>Vigna - chemistry</subject><subject>Vigna unguiculata</subject><issn>1612-1872</issn><issn>1612-1880</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFLwzAUh4Mobk6vHiXgxUu7JG2W9jirTqHiQJ3HkKbJ1pG2s1mV_vdmbk7w4un9eHzvB-8D4BwjHyNEhjLLP3yCCEEIhdEB6OMRJh6OInS4z4z0wIm1S8e7fXQMesGIshGjqA8mk2T4-AxFlcP0O00X3bqWC1UWUhg4roTpbGFhreGsmFcCttW8LWRrxFrA1IdvwqzgtM5PwZEWxqqz3RyA17vbl-TeS58mD8k49WSAgsjDWIQk1zrTVGZZGAhBBcokDmnIKEUUI8x0SLDUWEUZCnFMaOAyJYhpKfJgAK62vaumfm-VXfOysFIZIypVt5YTRmOnIgqYQy__oMu6bdxDG4qxOI5ZSB3lbynZ1NY2SvNVU5Si6ThGfKOYbxTzvWJ3cLGrbbNS5Xv8x6kD4i3wWRjV_VPHk-ub2W_5F2gthGs</recordid><startdate>202302</startdate><enddate>202302</enddate><creator>Mahavirsing Dinore, Jaysing</creator><creator>Shivaji Patil, Harshal</creator><creator>Farooqui, Samreen</creator><creator>Pradhan, Vidya</creator><creator>Farooqui, Mazahar</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6388-8663</orcidid><orcidid>https://orcid.org/0000-0002-0819-4984</orcidid></search><sort><creationdate>202302</creationdate><title>GC/MS and LC/MS Phytochemical Analysis of Vigna unguiculata L. Walp Pod</title><author>Mahavirsing Dinore, Jaysing ; Shivaji Patil, Harshal ; Farooqui, Samreen ; Pradhan, Vidya ; Farooqui, Mazahar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3038-11a42dffbf5cbb43aa5a0bc1454755051017f421cf1e8b0419253f1e5207fcad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Ampicillin</topic><topic>antimicrobial activity</topic><topic>Antimicrobial agents</topic><topic>antioxidant activity</topic><topic>Antioxidants - chemistry</topic><topic>Carotenoids</topic><topic>Chromatography</topic><topic>Chromatography, Liquid</topic><topic>Constituents</topic><topic>cowpea</topic><topic>Cowpeas</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Esters</topic><topic>Fatty acids</topic><topic>Flavonoids</topic><topic>Gas chromatography</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>GC/MS</topic><topic>LC/MS</topic><topic>Liquid chromatography</topic><topic>Mass Spectrometry</topic><topic>Mass spectroscopy</topic><topic>Metabolites</topic><topic>Methanol</topic><topic>Minimum inhibitory concentration</topic><topic>Oxidants</topic><topic>Oxidizing agents</topic><topic>Phenolic compounds</topic><topic>Phenols</topic><topic>Phytochemicals</topic><topic>Phytochemicals - chemistry</topic><topic>Plant Extracts - chemistry</topic><topic>Scavenging</topic><topic>Scientific imaging</topic><topic>Spectral analysis</topic><topic>Spectrum analysis</topic><topic>Vigna - chemistry</topic><topic>Vigna unguiculata</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mahavirsing Dinore, Jaysing</creatorcontrib><creatorcontrib>Shivaji Patil, Harshal</creatorcontrib><creatorcontrib>Farooqui, Samreen</creatorcontrib><creatorcontrib>Pradhan, Vidya</creatorcontrib><creatorcontrib>Farooqui, Mazahar</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chemistry & biodiversity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mahavirsing Dinore, Jaysing</au><au>Shivaji Patil, Harshal</au><au>Farooqui, Samreen</au><au>Pradhan, Vidya</au><au>Farooqui, Mazahar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GC/MS and LC/MS Phytochemical Analysis of Vigna unguiculata L. Walp Pod</atitle><jtitle>Chemistry & biodiversity</jtitle><addtitle>Chem Biodivers</addtitle><date>2023-02</date><risdate>2023</risdate><volume>20</volume><issue>2</issue><spage>e202200048</spage><epage>n/a</epage><pages>e202200048-n/a</pages><issn>1612-1872</issn><eissn>1612-1880</eissn><abstract>Vigna unguiculata (L. Walp) or Cowpea pod methanolic extracts phytochemical analysis, total phenolic content (TPC), and secondary metabolite profiling were determined using gas chromatography‐mass spectrometry (GC/MS) and liquid chromatography‐mass spectrometry (LC/MS) analysis. GC/MS analysis revealed twenty compounds in the extract, while LC/MS analysis identified twenty‐four compounds. GC/MS chromatogram analysis suggested the presence of opioid α‐N‐Normethadol a major constituent found in methanolic extract and fatty acid esters carotenoid is found second major constituent. LC/MS chromatogram and the mass spectral analysis demonstrated the presence of flavonoids, carotenoids, and alkaloids as major phytochemicals. We investigated the antibacterial, anti‐fungal, and anti‐oxidant activity of pod methanolic extract. The extract was found equally effective against E. coli, S. pyogenes, and P. aeruginosa with MIC 100 μg/mL similar to the standard Ampicillin (MIC 100 μg/mL). C. albicans were found to be most susceptible to Vign unguiculata pods methanolic extract with a MIC of 250 μg/mL. The pod extract showed significant DPPH scavenging activity (IC50=78.38±0.15) which suggests its antioxidant potential.</abstract><cop>Switzerland</cop><pub>Wiley Subscription Services, Inc</pub><pmid>36576750</pmid><doi>10.1002/cbdv.202200048</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-6388-8663</orcidid><orcidid>https://orcid.org/0000-0002-0819-4984</orcidid></addata></record> |
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| subjects | Ampicillin antimicrobial activity Antimicrobial agents antioxidant activity Antioxidants - chemistry Carotenoids Chromatography Chromatography, Liquid Constituents cowpea Cowpeas E coli Escherichia coli Esters Fatty acids Flavonoids Gas chromatography Gas Chromatography-Mass Spectrometry GC/MS LC/MS Liquid chromatography Mass Spectrometry Mass spectroscopy Metabolites Methanol Minimum inhibitory concentration Oxidants Oxidizing agents Phenolic compounds Phenols Phytochemicals Phytochemicals - chemistry Plant Extracts - chemistry Scavenging Scientific imaging Spectral analysis Spectrum analysis Vigna - chemistry Vigna unguiculata |
| title | GC/MS and LC/MS Phytochemical Analysis of Vigna unguiculata L. Walp Pod |
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