Better Cryo-EM Specimen Preparation: How to Deal with the Air–Water Interface?

[Display omitted] •We outline the nature and impacts of air–water interface (AWI) during cryo-EM specimen preparation.•AWI poses risks of unpredictable particle distribution, protein denaturation, complex dissociation and preferential orientation.•The review overviews various strategies to deal with...

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Veröffentlicht in:	Journal of molecular biology 2023-05, Vol.435 (9), p.167926-167926, Article 167926
Hauptverfasser:	Liu, Nan, Wang, Hong-Wei
Format:	Artikel
Sprache:	eng
Schlagworte:	air–water interface Cryo-EM Cryoelectron Microscopy graphene Graphite - chemistry Microscopy, Electron preferential orientation protein denaturation Specimen Handling Water - chemistry
Online-Zugang:	Volltext
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