Enhancing CHO cell productivity through a dual selection system using Aspg and Gs in glutamine free medium

The dominant method for generating Chinese hamster ovary (CHO) cell lines that produce high titers of biotherapeutic proteins utilizes selectable markers such as dihydrofolate reductase (Dhfr) or glutamine synthetase (Gs), alongside inhibitory compounds like methotrexate or methionine sulfoximine, r...

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Veröffentlicht in:	Biotechnology and bioengineering 2023-04, Vol.120 (4), p.1159-1166
Hauptverfasser:	Ha, Tae Kwang, Òdena, Andreu, Karottki, Karen Julie la Cour, Kim, Che Lin, Hefzi, Hooman, Lee, Gyun Min, Faustrup Kildegaard, Helene, Nielsen, Lars K., Grav, Lise Marie, Lewis, Nathan E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Asparaginase biotherapeutic production Cell culture Cell lines Chinese hamster ovary cells CHO Cells Cricetinae Cricetulus Dihydrofolate reductase Etanercept Glutamate-ammonia ligase Glutamate-Ammonia Ligase - genetics Glutamate-Ammonia Ligase - metabolism Glutamine Glutamine - metabolism Glutamine - pharmacology glutamine synthetase Growth rate Industrial production mammalian cell line Methionine Methotrexate Optimization Plasmids Productivity Recombinant Proteins - genetics Reductases selection system Transfection
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