Determination of steroid hormones in grey seal (Halichoerus grypus) blood plasma using convergence chromatography tandem mass spectrometry
A hybrid solid phase extraction (HybridSPE) protocol tailored to ultra-performance convergence chromatography tandem mass spectrometry (UPC2-MS/MS) was developed for the determination of 19 steroid hormones in grey seal (Halichoerus grypus) blood plasma. In this study, the protocol demonstrated acce...
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| Veröffentlicht in: | Talanta (Oxford) 2023-03, Vol.254, p.124109-124109, Article 124109 |
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| creator | Sait, Shannen Thora Lea Survilienė, Vaida Jüssi, Mart Gonzalez, Susana V. Ciesielski, Tomasz Maciej Jenssen, Bjørn Munro Asimakopoulos, Alexandros G. |
| description | A hybrid solid phase extraction (HybridSPE) protocol tailored to ultra-performance convergence chromatography tandem mass spectrometry (UPC2-MS/MS) was developed for the determination of 19 steroid hormones in grey seal (Halichoerus grypus) blood plasma. In this study, the protocol demonstrated acceptable absolute recoveries ranging from 33 to 90%. The chromatographic separation was carried out using a gradient elution program with a total run time of 5 min. For most target analytes, the method repeatability ranged from 1.9 to 24% and the method limits of quantification (mLOQs) ranged from 0.03 to 1.67 ng/mL. A total of 9 plasma samples were analysed to demonstrate the applicability of the developed method, and 13 steroid hormones were quantified in grey seal pup plasma. The most prevalent steroids: cortisol, cortisone, corticosterone, 11-deoxycortisol, progesterone and 17α-hydroxyprogesterone were detected at concentrations in the range of 12.6–40.1, 7.10–24.2, 0.74–10.7, 1.06–5.72, 0.38–4.38 and |
| doi_str_mv | 10.1016/j.talanta.2022.124109 |
| format | Article |
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[Display omitted]
•First study to determine steroid hormones in the plasma of pinnipeds using convergence chromatography.•Occurrence profiles of steroid hormones in grey seal pup plasma were presented.•Rapid chromatographic separation of 19 steroid hormones was achieved within 5 min.•Rapid HybridSPE protocol was developed for the extraction of the steroids from plasma.•Testosterone and 17β-estradiol concentrations were cross-array compared with radioimmunoassays.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2022.124109</identifier><identifier>PMID: 36535212</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Chromatography, High Pressure Liquid - methods ; Chromatography, Liquid - methods ; Convergence chromatography ; Cortisol ; Hydrocortisone ; Limit of Detection ; Marine mammals ; Pinnipeds ; Plasma - chemistry ; Seals, Earless ; Steroids - analysis ; Tandem Mass Spectrometry - methods</subject><ispartof>Talanta (Oxford), 2023-03, Vol.254, p.124109-124109, Article 124109</ispartof><rights>2022 The Authors</rights><rights>Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-e661286650755123d2fab17b356dc99ec2d7128d160793c2e6cffe084f8a54a03</citedby><cites>FETCH-LOGICAL-c412t-e661286650755123d2fab17b356dc99ec2d7128d160793c2e6cffe084f8a54a03</cites><orcidid>0000-0001-5241-9290</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.talanta.2022.124109$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36535212$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sait, Shannen Thora Lea</creatorcontrib><creatorcontrib>Survilienė, Vaida</creatorcontrib><creatorcontrib>Jüssi, Mart</creatorcontrib><creatorcontrib>Gonzalez, Susana V.</creatorcontrib><creatorcontrib>Ciesielski, Tomasz Maciej</creatorcontrib><creatorcontrib>Jenssen, Bjørn Munro</creatorcontrib><creatorcontrib>Asimakopoulos, Alexandros G.</creatorcontrib><title>Determination of steroid hormones in grey seal (Halichoerus grypus) blood plasma using convergence chromatography tandem mass spectrometry</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>A hybrid solid phase extraction (HybridSPE) protocol tailored to ultra-performance convergence chromatography tandem mass spectrometry (UPC2-MS/MS) was developed for the determination of 19 steroid hormones in grey seal (Halichoerus grypus) blood plasma. In this study, the protocol demonstrated acceptable absolute recoveries ranging from 33 to 90%. The chromatographic separation was carried out using a gradient elution program with a total run time of 5 min. For most target analytes, the method repeatability ranged from 1.9 to 24% and the method limits of quantification (mLOQs) ranged from 0.03 to 1.67 ng/mL. A total of 9 plasma samples were analysed to demonstrate the applicability of the developed method, and 13 steroid hormones were quantified in grey seal pup plasma. The most prevalent steroids: cortisol, cortisone, corticosterone, 11-deoxycortisol, progesterone and 17α-hydroxyprogesterone were detected at concentrations in the range of 12.6–40.1, 7.10–24.2, 0.74–10.7, 1.06–5.72, 0.38–4.38 and <mLOQ - 1.01 ng/mL, respectively. To our knowledge, this is the first study to determine steroid hormones in the plasma of pinnipeds using convergence chromatography.
[Display omitted]
•First study to determine steroid hormones in the plasma of pinnipeds using convergence chromatography.•Occurrence profiles of steroid hormones in grey seal pup plasma were presented.•Rapid chromatographic separation of 19 steroid hormones was achieved within 5 min.•Rapid HybridSPE protocol was developed for the extraction of the steroids from plasma.•Testosterone and 17β-estradiol concentrations were cross-array compared with radioimmunoassays.</description><subject>Animals</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Chromatography, Liquid - methods</subject><subject>Convergence chromatography</subject><subject>Cortisol</subject><subject>Hydrocortisone</subject><subject>Limit of Detection</subject><subject>Marine mammals</subject><subject>Pinnipeds</subject><subject>Plasma - chemistry</subject><subject>Seals, Earless</subject><subject>Steroids - analysis</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctuFDEQRS0EIkPgE0BehkUPfrT7sUIogQQpEhtYWx67esajdrtxuSP1L_DVeDQD26xKVT5VV76XkPecbTnjzafjNpvRTNlsBRNiy0XNWf-CbHjXykqqVr4kG8ZkX_W8ZlfkDeKRMSYkk6_JlWyUVIKLDflzBxlS8JPJPk40DhRLH72jh5hCnACpn-g-wUoRzEhvHszo7SFCWrCM13nBj3Q3xujoPBoMhi7opz21cXqCtIfJArWHFIPJcZ_MfFhpNpODQINBpDiDzeUVclrfkleDGRHeXeo1-fXt68_bh-rxx_332y-Pla25yBU0DRdd0yjWKsWFdGIwO97upGqc7XuwwrUFcLxhbS-tgMYOA7CuHjqjasPkNbk5351T_L0AZh08WhiLmxAX1KJVRUGIriuoOqM2RcQEg56TDyatmjN9ikEf9SUGfYpBn2Moex8uEssugPu_9c_3Anw-A1A--uQhabT-ZJbzqTiiXfTPSPwFk-yeag</recordid><startdate>20230301</startdate><enddate>20230301</enddate><creator>Sait, Shannen Thora Lea</creator><creator>Survilienė, Vaida</creator><creator>Jüssi, Mart</creator><creator>Gonzalez, Susana V.</creator><creator>Ciesielski, Tomasz Maciej</creator><creator>Jenssen, Bjørn Munro</creator><creator>Asimakopoulos, Alexandros G.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5241-9290</orcidid></search><sort><creationdate>20230301</creationdate><title>Determination of steroid hormones in grey seal (Halichoerus grypus) blood plasma using convergence chromatography tandem mass spectrometry</title><author>Sait, Shannen Thora Lea ; Survilienė, Vaida ; Jüssi, Mart ; Gonzalez, Susana V. ; Ciesielski, Tomasz Maciej ; Jenssen, Bjørn Munro ; Asimakopoulos, Alexandros G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-e661286650755123d2fab17b356dc99ec2d7128d160793c2e6cffe084f8a54a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Chromatography, Liquid - methods</topic><topic>Convergence chromatography</topic><topic>Cortisol</topic><topic>Hydrocortisone</topic><topic>Limit of Detection</topic><topic>Marine mammals</topic><topic>Pinnipeds</topic><topic>Plasma - chemistry</topic><topic>Seals, Earless</topic><topic>Steroids - analysis</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sait, Shannen Thora Lea</creatorcontrib><creatorcontrib>Survilienė, Vaida</creatorcontrib><creatorcontrib>Jüssi, Mart</creatorcontrib><creatorcontrib>Gonzalez, Susana V.</creatorcontrib><creatorcontrib>Ciesielski, Tomasz Maciej</creatorcontrib><creatorcontrib>Jenssen, Bjørn Munro</creatorcontrib><creatorcontrib>Asimakopoulos, Alexandros G.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sait, Shannen Thora Lea</au><au>Survilienė, Vaida</au><au>Jüssi, Mart</au><au>Gonzalez, Susana V.</au><au>Ciesielski, Tomasz Maciej</au><au>Jenssen, Bjørn Munro</au><au>Asimakopoulos, Alexandros G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of steroid hormones in grey seal (Halichoerus grypus) blood plasma using convergence chromatography tandem mass spectrometry</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2023-03-01</date><risdate>2023</risdate><volume>254</volume><spage>124109</spage><epage>124109</epage><pages>124109-124109</pages><artnum>124109</artnum><issn>0039-9140</issn><eissn>1873-3573</eissn><abstract>A hybrid solid phase extraction (HybridSPE) protocol tailored to ultra-performance convergence chromatography tandem mass spectrometry (UPC2-MS/MS) was developed for the determination of 19 steroid hormones in grey seal (Halichoerus grypus) blood plasma. In this study, the protocol demonstrated acceptable absolute recoveries ranging from 33 to 90%. The chromatographic separation was carried out using a gradient elution program with a total run time of 5 min. For most target analytes, the method repeatability ranged from 1.9 to 24% and the method limits of quantification (mLOQs) ranged from 0.03 to 1.67 ng/mL. A total of 9 plasma samples were analysed to demonstrate the applicability of the developed method, and 13 steroid hormones were quantified in grey seal pup plasma. The most prevalent steroids: cortisol, cortisone, corticosterone, 11-deoxycortisol, progesterone and 17α-hydroxyprogesterone were detected at concentrations in the range of 12.6–40.1, 7.10–24.2, 0.74–10.7, 1.06–5.72, 0.38–4.38 and <mLOQ - 1.01 ng/mL, respectively. To our knowledge, this is the first study to determine steroid hormones in the plasma of pinnipeds using convergence chromatography.
[Display omitted]
•First study to determine steroid hormones in the plasma of pinnipeds using convergence chromatography.•Occurrence profiles of steroid hormones in grey seal pup plasma were presented.•Rapid chromatographic separation of 19 steroid hormones was achieved within 5 min.•Rapid HybridSPE protocol was developed for the extraction of the steroids from plasma.•Testosterone and 17β-estradiol concentrations were cross-array compared with radioimmunoassays.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>36535212</pmid><doi>10.1016/j.talanta.2022.124109</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-5241-9290</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Talanta (Oxford), 2023-03, Vol.254, p.124109-124109, Article 124109 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Chromatography, High Pressure Liquid - methods Chromatography, Liquid - methods Convergence chromatography Cortisol Hydrocortisone Limit of Detection Marine mammals Pinnipeds Plasma - chemistry Seals, Earless Steroids - analysis Tandem Mass Spectrometry - methods |
| title | Determination of steroid hormones in grey seal (Halichoerus grypus) blood plasma using convergence chromatography tandem mass spectrometry |
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