Small ruminant lentivirus capsid protein (SRLV-p25) antigenic structural prediction and immunogenicity to recombinant SRLV-rp25-coupled to immunostimulatory complexes based on glycyrrhizinic acid
ABSTRACT Small ruminant lentiviruses (SRLV) infect sheep and goats resulting in significant economic losses. This study evaluated for the first time the predicted conformational structure of the SRLV-capsid-protein 25 (SRLV-p25) and analyzed the antigenicity of recombinant protein (SRLV-rp25) in mic...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2023-02, Vol.87 (3), p.267-278 |
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| creator | Castañeda-Montes, María Azucena Cuevas-Romero, Julieta Sandra Cerriteño-Sánchez, José Luis de María Ávila-De la Vega, Lucero García-Cambrón, José Bryan Ramírez-Álvarez, Hugo |
| description | ABSTRACT
Small ruminant lentiviruses (SRLV) infect sheep and goats resulting in significant economic losses. This study evaluated for the first time the predicted conformational structure of the SRLV-capsid-protein 25 (SRLV-p25) and analyzed the antigenicity of recombinant protein (SRLV-rp25) in mice by coupling to an immunostimulatory complexes based on glycyrrhizinic acid liposomes (GAL) and tested plasma from goats and sheep naturally infected. Analysis in silico and conformational structure of SRLV-p25 (genotype B-FESC-752) showed similar characteristics to other lentiviral capsids. The efficient expression of SRLV-rp25 was confirmed by Western blot. The humoral immune responses in mice showed an increased level of antibodies from day 21 to 35 of the SRLV-rp25-GAL and SRLV-rp25-ISCOM® groups and the cellular immune response showed no significant difference in IL-10 levels (P >.05), however, a significant difference (P |
| doi_str_mv | 10.1093/bbb/zbac206 |
| format | Article |
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Small ruminant lentiviruses (SRLV) infect sheep and goats resulting in significant economic losses. This study evaluated for the first time the predicted conformational structure of the SRLV-capsid-protein 25 (SRLV-p25) and analyzed the antigenicity of recombinant protein (SRLV-rp25) in mice by coupling to an immunostimulatory complexes based on glycyrrhizinic acid liposomes (GAL) and tested plasma from goats and sheep naturally infected. Analysis in silico and conformational structure of SRLV-p25 (genotype B-FESC-752) showed similar characteristics to other lentiviral capsids. The efficient expression of SRLV-rp25 was confirmed by Western blot. The humoral immune responses in mice showed an increased level of antibodies from day 21 to 35 of the SRLV-rp25-GAL and SRLV-rp25-ISCOM® groups and the cellular immune response showed no significant difference in IL-10 levels (P >.05), however, a significant difference (P <.001) was observed when comparing SRLV-rp25-GAL with SRLV-rp25 groups. Immunoreactivity toward SRLV-rp25 revealed 61% of positive samples from naturally infected goats and sheep.
Graphical Abstract
Graphical Abstract
Complexes formed by glycyrrhizinic acid liposomes and the recombinant protein SRLV-rp25 obtained from genotype B SRLV (SRLV-rp25-GAL) efficiently increase the humoral response in CF-1 mice.</description><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1093/bbb/zbac206</identifier><identifier>PMID: 36535645</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Animals ; Capsid Proteins - genetics ; Glycyrrhizic Acid ; Goats ; Lentivirus - genetics ; Lentivirus Infections - veterinary ; Mice ; Phylogeny ; Ruminants ; Sheep ; Sheep Diseases</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2023-02, Vol.87 (3), p.267-278</ispartof><rights>The Author(s) 2022. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry. 2022</rights><rights>The Author(s) 2022. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0003-1682-8104</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,1579,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36535645$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Castañeda-Montes, María Azucena</creatorcontrib><creatorcontrib>Cuevas-Romero, Julieta Sandra</creatorcontrib><creatorcontrib>Cerriteño-Sánchez, José Luis</creatorcontrib><creatorcontrib>de María Ávila-De la Vega, Lucero</creatorcontrib><creatorcontrib>García-Cambrón, José Bryan</creatorcontrib><creatorcontrib>Ramírez-Álvarez, Hugo</creatorcontrib><title>Small ruminant lentivirus capsid protein (SRLV-p25) antigenic structural prediction and immunogenicity to recombinant SRLV-rp25-coupled to immunostimulatory complexes based on glycyrrhizinic acid</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>ABSTRACT
Small ruminant lentiviruses (SRLV) infect sheep and goats resulting in significant economic losses. This study evaluated for the first time the predicted conformational structure of the SRLV-capsid-protein 25 (SRLV-p25) and analyzed the antigenicity of recombinant protein (SRLV-rp25) in mice by coupling to an immunostimulatory complexes based on glycyrrhizinic acid liposomes (GAL) and tested plasma from goats and sheep naturally infected. Analysis in silico and conformational structure of SRLV-p25 (genotype B-FESC-752) showed similar characteristics to other lentiviral capsids. The efficient expression of SRLV-rp25 was confirmed by Western blot. The humoral immune responses in mice showed an increased level of antibodies from day 21 to 35 of the SRLV-rp25-GAL and SRLV-rp25-ISCOM® groups and the cellular immune response showed no significant difference in IL-10 levels (P >.05), however, a significant difference (P <.001) was observed when comparing SRLV-rp25-GAL with SRLV-rp25 groups. Immunoreactivity toward SRLV-rp25 revealed 61% of positive samples from naturally infected goats and sheep.
Graphical Abstract
Graphical Abstract
Complexes formed by glycyrrhizinic acid liposomes and the recombinant protein SRLV-rp25 obtained from genotype B SRLV (SRLV-rp25-GAL) efficiently increase the humoral response in CF-1 mice.</description><subject>Animals</subject><subject>Capsid Proteins - genetics</subject><subject>Glycyrrhizic Acid</subject><subject>Goats</subject><subject>Lentivirus - genetics</subject><subject>Lentivirus Infections - veterinary</subject><subject>Mice</subject><subject>Phylogeny</subject><subject>Ruminants</subject><subject>Sheep</subject><subject>Sheep Diseases</subject><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kUtv1DAUhS0kREthxR55hcoi1I_YiZeo4iWNhESBbWRfe4orOwl-INK_xx_D0xlY3cX57rmPg9ALSt5QoviVMebq3mhgRD5C55T3QydVP5yhpznfEUIUFfQJOuNScCF7cY7-3EQdAk41-lnPBQc3F__Lp5ox6DV7i9e0FOdnfHnzZfe9W5l4jRvob93sAeeSKpSadGicsx6KX-amW-xjrPPyQPmy4bLg5GCJ5jjmwSs1sw6WugZnD8CxJRcfa9BlSRtuDU387TI2Ojeoed-GDbaUfvh7f1hAg7fP0OO9Dtk9P9UL9O39u6_XH7vd5w-frt_uujsmZemEFr0QxAAfmRAgjVZ0UGC42TtimeLDODIlpRycsk5YMe57N4LZSwPQK-AX6PLo217ys7pcpugzuBD07JaaJzYISRmjfd_Qlye0mujstCYfddqmf49vwKsj0M7_r1IyHVKcWorTKUX-F5UvlhY</recordid><startdate>20230224</startdate><enddate>20230224</enddate><creator>Castañeda-Montes, María Azucena</creator><creator>Cuevas-Romero, Julieta Sandra</creator><creator>Cerriteño-Sánchez, José Luis</creator><creator>de María Ávila-De la Vega, Lucero</creator><creator>García-Cambrón, José Bryan</creator><creator>Ramírez-Álvarez, Hugo</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1682-8104</orcidid></search><sort><creationdate>20230224</creationdate><title>Small ruminant lentivirus capsid protein (SRLV-p25) antigenic structural prediction and immunogenicity to recombinant SRLV-rp25-coupled to immunostimulatory complexes based on glycyrrhizinic acid</title><author>Castañeda-Montes, María Azucena ; Cuevas-Romero, Julieta Sandra ; Cerriteño-Sánchez, José Luis ; de María Ávila-De la Vega, Lucero ; García-Cambrón, José Bryan ; Ramírez-Álvarez, Hugo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j266t-5a54550bc38255c6ba9179cb3bfe0d293788296667e9de5d58f4e8cbf6bcc49c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Capsid Proteins - genetics</topic><topic>Glycyrrhizic Acid</topic><topic>Goats</topic><topic>Lentivirus - genetics</topic><topic>Lentivirus Infections - veterinary</topic><topic>Mice</topic><topic>Phylogeny</topic><topic>Ruminants</topic><topic>Sheep</topic><topic>Sheep Diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Castañeda-Montes, María Azucena</creatorcontrib><creatorcontrib>Cuevas-Romero, Julieta Sandra</creatorcontrib><creatorcontrib>Cerriteño-Sánchez, José Luis</creatorcontrib><creatorcontrib>de María Ávila-De la Vega, Lucero</creatorcontrib><creatorcontrib>García-Cambrón, José Bryan</creatorcontrib><creatorcontrib>Ramírez-Álvarez, Hugo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Castañeda-Montes, María Azucena</au><au>Cuevas-Romero, Julieta Sandra</au><au>Cerriteño-Sánchez, José Luis</au><au>de María Ávila-De la Vega, Lucero</au><au>García-Cambrón, José Bryan</au><au>Ramírez-Álvarez, Hugo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Small ruminant lentivirus capsid protein (SRLV-p25) antigenic structural prediction and immunogenicity to recombinant SRLV-rp25-coupled to immunostimulatory complexes based on glycyrrhizinic acid</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2023-02-24</date><risdate>2023</risdate><volume>87</volume><issue>3</issue><spage>267</spage><epage>278</epage><pages>267-278</pages><eissn>1347-6947</eissn><abstract>ABSTRACT
Small ruminant lentiviruses (SRLV) infect sheep and goats resulting in significant economic losses. This study evaluated for the first time the predicted conformational structure of the SRLV-capsid-protein 25 (SRLV-p25) and analyzed the antigenicity of recombinant protein (SRLV-rp25) in mice by coupling to an immunostimulatory complexes based on glycyrrhizinic acid liposomes (GAL) and tested plasma from goats and sheep naturally infected. Analysis in silico and conformational structure of SRLV-p25 (genotype B-FESC-752) showed similar characteristics to other lentiviral capsids. The efficient expression of SRLV-rp25 was confirmed by Western blot. The humoral immune responses in mice showed an increased level of antibodies from day 21 to 35 of the SRLV-rp25-GAL and SRLV-rp25-ISCOM® groups and the cellular immune response showed no significant difference in IL-10 levels (P >.05), however, a significant difference (P <.001) was observed when comparing SRLV-rp25-GAL with SRLV-rp25 groups. Immunoreactivity toward SRLV-rp25 revealed 61% of positive samples from naturally infected goats and sheep.
Graphical Abstract
Graphical Abstract
Complexes formed by glycyrrhizinic acid liposomes and the recombinant protein SRLV-rp25 obtained from genotype B SRLV (SRLV-rp25-GAL) efficiently increase the humoral response in CF-1 mice.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>36535645</pmid><doi>10.1093/bbb/zbac206</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-1682-8104</orcidid></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 2023-02, Vol.87 (3), p.267-278 |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Animals Capsid Proteins - genetics Glycyrrhizic Acid Goats Lentivirus - genetics Lentivirus Infections - veterinary Mice Phylogeny Ruminants Sheep Sheep Diseases |
| title | Small ruminant lentivirus capsid protein (SRLV-p25) antigenic structural prediction and immunogenicity to recombinant SRLV-rp25-coupled to immunostimulatory complexes based on glycyrrhizinic acid |
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