Empowering gene delivery with protein engineering platforms
The repertoire of therapeutic proteins has been substantially augmented by molecular engineering approaches, which have seen remarkable advancement in recent years. In particular, advances in directed evolution technologies have empowered the development of custom-designed proteins with novel and di...
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| Veröffentlicht in: | Gene therapy 2023-12, Vol.30 (12), p.775-782 |
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| creator | Kizerwetter, Monika Pietz, Kevin Tomasovic, Luke M. Spangler, Jamie B. |
| description | The repertoire of therapeutic proteins has been substantially augmented by molecular engineering approaches, which have seen remarkable advancement in recent years. In particular, advances in directed evolution technologies have empowered the development of custom-designed proteins with novel and disease-relevant functions. Whereas engineered proteins have typically been administered through systemic injection of the purified molecule, exciting progress in gene delivery affords the opportunity to elicit sustained production of the engineered proteins by targeted cells in the host organism. Combining developments at the leading edge of protein engineering and gene delivery has catapulted a new wave of molecular and cellular therapy approaches, which harbor great promise for personalized and precision medicine. This mini-review outlines currently used display platforms for protein evolution and describes recent examples of how the resulting engineered proteins have been incorporated into DNA- and cell-based therapeutic platforms, both in vitro and in vivo. Collectively, the strategies detailed herein provide a framework for synthesizing molecular engineering workflows with gene therapy systems for a breadth of applications in research and medicine. |
| doi_str_mv | 10.1038/s41434-022-00379-6 |
| format | Article |
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| subjects | 13 13/21 42 42/41 631/154/51 631/154/51/1844 631/250/2520 631/61/350 Biomedical and Life Sciences Biomedicine Cell Biology Directed evolution Engineering Gene Expression Gene Therapy Gene transfer Gene Transfer Techniques Genetic Therapy Human Genetics Nanotechnology Power, Psychological Precision Medicine Protein Engineering Protein synthesis Proteins Review Article Therapeutic communication |
| title | Empowering gene delivery with protein engineering platforms |
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