Pyrogallol downregulates the expression of virulence-associated proteins in Acinetobacter baumannii and showing anti-infection activity by improving non-specific immune response in zebrafish model
Acinetobacter baumannii, a virulent uropathogen with widespread antibiotic resistance, has arisen as a critical scientific challenge, necessitating the development of innovative therapeutic agents. This is the first study reveal the proteomic changes in A. baumannii upon pyrogallol treatment for und...
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| Veröffentlicht in: | International journal of biological macromolecules 2023-01, Vol.226, p.853-869 |
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| creator | Abirami, Gurusamy Alexpandi, Rajaiah Sudhin, Sadasivan Durgadevi, Ravindran Roshni, Prithiviraj Swasthikka Kumar, Ponnuchamy Veera Ravi, Arumugam |
| description | Acinetobacter baumannii, a virulent uropathogen with widespread antibiotic resistance, has arisen as a critical scientific challenge, necessitating the development of innovative therapeutic agents. This is the first study reveal the proteomic changes in A. baumannii upon pyrogallol treatment for understanding the mechanisms using nano-LC-MS/MS-based quantitative proteomics and qPCR analysis. The obtained results found that pyrogallol treatment dramatically downregulated the expression level of several key proteins such as GroEL, DnaK, ClpB, SodB, KatE, Bap, CsuA/B, PgaA, PgaC, BfmR, OmpA, and SecA in A. baumannii, which are involved in chaperone-mediated oxidative stress responses, antioxidant defence system, biofilm formation, virulence enzyme production, bacterial adhesion, capsule formation, and antibiotic resistance. Accordingly, the pyrogallol dramatically enhanced the lifespan of A. baumannii-infected zebrafish by inhibiting bacterial colonization, demonstrating the anti-infective potential of pyrogallol against A. baumannii. Further, the histopathological results also demonstrated the disease protection efficacy of pyrogallol against the pathognomonic sign of A. baumannii infection. In addition, the pyrogallol treatment effectively improved the immune parameters such as serum myeloperoxidase activity, leukocyte respiratory burst activity, and serum lysozyme activity in zebrafish against A. baumannii infection. Based on the results, the present study strongly proposes pyrogallol as a promising therapeutic agent for treating A. baumannii infection.
•First study on proteomic changes in A. baumannii upon pyrogallol treatment.•Pyrogallol downregulated the expression level of several key proteins in A. baumannii.•Proteomic results were further validated by qPCR analysis and bioassays.•First study on anti-infective potential of pyrogallol against A. baumannii in zebrafish model.•Pyrogallol treatment improved the immune parameters in zebrafish. |
| doi_str_mv | 10.1016/j.ijbiomac.2022.12.045 |
| format | Article |
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•First study on proteomic changes in A. baumannii upon pyrogallol treatment.•Pyrogallol downregulated the expression level of several key proteins in A. baumannii.•Proteomic results were further validated by qPCR analysis and bioassays.•First study on anti-infective potential of pyrogallol against A. baumannii in zebrafish model.•Pyrogallol treatment improved the immune parameters in zebrafish.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2022.12.045</identifier><identifier>PMID: 36526063</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Acinetobacter baumannii - metabolism ; Animals ; Anti-Bacterial Agents - metabolism ; Anti-Bacterial Agents - pharmacology ; Anti-infective activity ; Anti-Infective Agents - metabolism ; Antivirulence activity ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biofilms ; Immunity ; Proteomics ; Pyrogallol - metabolism ; Pyrogallol - pharmacology ; Quantitative proteomics ; Tandem Mass Spectrometry ; Virulence ; Zebrafish - metabolism</subject><ispartof>International journal of biological macromolecules, 2023-01, Vol.226, p.853-869</ispartof><rights>2022</rights><rights>Copyright © 2022. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-dd80d7f30c45a21e66c8135df1b81a40b705fed58643c03fdd36eb55223117b03</citedby><cites>FETCH-LOGICAL-c368t-dd80d7f30c45a21e66c8135df1b81a40b705fed58643c03fdd36eb55223117b03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijbiomac.2022.12.045$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36526063$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abirami, Gurusamy</creatorcontrib><creatorcontrib>Alexpandi, Rajaiah</creatorcontrib><creatorcontrib>Sudhin, Sadasivan</creatorcontrib><creatorcontrib>Durgadevi, Ravindran</creatorcontrib><creatorcontrib>Roshni, Prithiviraj Swasthikka</creatorcontrib><creatorcontrib>Kumar, Ponnuchamy</creatorcontrib><creatorcontrib>Veera Ravi, Arumugam</creatorcontrib><title>Pyrogallol downregulates the expression of virulence-associated proteins in Acinetobacter baumannii and showing anti-infection activity by improving non-specific immune response in zebrafish model</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Acinetobacter baumannii, a virulent uropathogen with widespread antibiotic resistance, has arisen as a critical scientific challenge, necessitating the development of innovative therapeutic agents. This is the first study reveal the proteomic changes in A. baumannii upon pyrogallol treatment for understanding the mechanisms using nano-LC-MS/MS-based quantitative proteomics and qPCR analysis. The obtained results found that pyrogallol treatment dramatically downregulated the expression level of several key proteins such as GroEL, DnaK, ClpB, SodB, KatE, Bap, CsuA/B, PgaA, PgaC, BfmR, OmpA, and SecA in A. baumannii, which are involved in chaperone-mediated oxidative stress responses, antioxidant defence system, biofilm formation, virulence enzyme production, bacterial adhesion, capsule formation, and antibiotic resistance. Accordingly, the pyrogallol dramatically enhanced the lifespan of A. baumannii-infected zebrafish by inhibiting bacterial colonization, demonstrating the anti-infective potential of pyrogallol against A. baumannii. Further, the histopathological results also demonstrated the disease protection efficacy of pyrogallol against the pathognomonic sign of A. baumannii infection. In addition, the pyrogallol treatment effectively improved the immune parameters such as serum myeloperoxidase activity, leukocyte respiratory burst activity, and serum lysozyme activity in zebrafish against A. baumannii infection. Based on the results, the present study strongly proposes pyrogallol as a promising therapeutic agent for treating A. baumannii infection.
•First study on proteomic changes in A. baumannii upon pyrogallol treatment.•Pyrogallol downregulated the expression level of several key proteins in A. baumannii.•Proteomic results were further validated by qPCR analysis and bioassays.•First study on anti-infective potential of pyrogallol against A. baumannii in zebrafish model.•Pyrogallol treatment improved the immune parameters in zebrafish.</description><subject>Acinetobacter baumannii - metabolism</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - metabolism</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Anti-infective activity</subject><subject>Anti-Infective Agents - metabolism</subject><subject>Antivirulence activity</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biofilms</subject><subject>Immunity</subject><subject>Proteomics</subject><subject>Pyrogallol - metabolism</subject><subject>Pyrogallol - pharmacology</subject><subject>Quantitative proteomics</subject><subject>Tandem Mass Spectrometry</subject><subject>Virulence</subject><subject>Zebrafish - metabolism</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuO1DAQRS0EYpqGXxh5ySbBj9gJO0YjXtJIsIC15diV7moldmMnPdN8Hx-Go55hy8pW6da9VXUIueas5ozrd4caDz3GybpaMCFqLmrWqGdkw7v2fcUYk8_JhvGGVx2X7Iq8yvlQqlrx7iW5kloJzbTckD_fzynu7DjGkfp4HxLsltHOkOm8BwoPxwQ5Yww0DvSEaRkhOKhsztFhkXl6THEGDJlioDcOA8yxt26GRHu7TDYERGqDp3kf7zHsyn_GCsMAbl5tixRPOJ9pf6Y4FbPTKgoxVPkIDgd0pTwtAWgZ5BhDhjXoN_TJDpj3dIoextfkxWDHDG8e3y35-enjj9sv1d23z19vb-4qJ3U3V953zLeDZK5RVnDQ2pXjKD_wvuO2YX3L1ABedbqRjsnBe6mhV0oIyXnbM7klby--Zc5fC-TZTJgdjKMNEJdsRKuUarUol90SfZG6FHNOMJhjwsmms-HMrATNwTwRNCtBw4UpBEvj9WPG0k_g_7U9ISuCDxcBlE1PCMlkhysVj6kc1fiI_8v4C-fStto</recordid><startdate>20230131</startdate><enddate>20230131</enddate><creator>Abirami, Gurusamy</creator><creator>Alexpandi, Rajaiah</creator><creator>Sudhin, Sadasivan</creator><creator>Durgadevi, Ravindran</creator><creator>Roshni, Prithiviraj Swasthikka</creator><creator>Kumar, Ponnuchamy</creator><creator>Veera Ravi, Arumugam</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20230131</creationdate><title>Pyrogallol downregulates the expression of virulence-associated proteins in Acinetobacter baumannii and showing anti-infection activity by improving non-specific immune response in zebrafish model</title><author>Abirami, Gurusamy ; Alexpandi, Rajaiah ; Sudhin, Sadasivan ; Durgadevi, Ravindran ; Roshni, Prithiviraj Swasthikka ; Kumar, Ponnuchamy ; Veera Ravi, Arumugam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-dd80d7f30c45a21e66c8135df1b81a40b705fed58643c03fdd36eb55223117b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Acinetobacter baumannii - metabolism</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - metabolism</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Anti-infective activity</topic><topic>Anti-Infective Agents - metabolism</topic><topic>Antivirulence activity</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biofilms</topic><topic>Immunity</topic><topic>Proteomics</topic><topic>Pyrogallol - metabolism</topic><topic>Pyrogallol - pharmacology</topic><topic>Quantitative proteomics</topic><topic>Tandem Mass Spectrometry</topic><topic>Virulence</topic><topic>Zebrafish - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abirami, Gurusamy</creatorcontrib><creatorcontrib>Alexpandi, Rajaiah</creatorcontrib><creatorcontrib>Sudhin, Sadasivan</creatorcontrib><creatorcontrib>Durgadevi, Ravindran</creatorcontrib><creatorcontrib>Roshni, Prithiviraj Swasthikka</creatorcontrib><creatorcontrib>Kumar, Ponnuchamy</creatorcontrib><creatorcontrib>Veera Ravi, Arumugam</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abirami, Gurusamy</au><au>Alexpandi, Rajaiah</au><au>Sudhin, Sadasivan</au><au>Durgadevi, Ravindran</au><au>Roshni, Prithiviraj Swasthikka</au><au>Kumar, Ponnuchamy</au><au>Veera Ravi, Arumugam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pyrogallol downregulates the expression of virulence-associated proteins in Acinetobacter baumannii and showing anti-infection activity by improving non-specific immune response in zebrafish model</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2023-01-31</date><risdate>2023</risdate><volume>226</volume><spage>853</spage><epage>869</epage><pages>853-869</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>Acinetobacter baumannii, a virulent uropathogen with widespread antibiotic resistance, has arisen as a critical scientific challenge, necessitating the development of innovative therapeutic agents. This is the first study reveal the proteomic changes in A. baumannii upon pyrogallol treatment for understanding the mechanisms using nano-LC-MS/MS-based quantitative proteomics and qPCR analysis. The obtained results found that pyrogallol treatment dramatically downregulated the expression level of several key proteins such as GroEL, DnaK, ClpB, SodB, KatE, Bap, CsuA/B, PgaA, PgaC, BfmR, OmpA, and SecA in A. baumannii, which are involved in chaperone-mediated oxidative stress responses, antioxidant defence system, biofilm formation, virulence enzyme production, bacterial adhesion, capsule formation, and antibiotic resistance. Accordingly, the pyrogallol dramatically enhanced the lifespan of A. baumannii-infected zebrafish by inhibiting bacterial colonization, demonstrating the anti-infective potential of pyrogallol against A. baumannii. Further, the histopathological results also demonstrated the disease protection efficacy of pyrogallol against the pathognomonic sign of A. baumannii infection. In addition, the pyrogallol treatment effectively improved the immune parameters such as serum myeloperoxidase activity, leukocyte respiratory burst activity, and serum lysozyme activity in zebrafish against A. baumannii infection. Based on the results, the present study strongly proposes pyrogallol as a promising therapeutic agent for treating A. baumannii infection.
•First study on proteomic changes in A. baumannii upon pyrogallol treatment.•Pyrogallol downregulated the expression level of several key proteins in A. baumannii.•Proteomic results were further validated by qPCR analysis and bioassays.•First study on anti-infective potential of pyrogallol against A. baumannii in zebrafish model.•Pyrogallol treatment improved the immune parameters in zebrafish.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>36526063</pmid><doi>10.1016/j.ijbiomac.2022.12.045</doi><tpages>17</tpages></addata></record> |
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| subjects | Acinetobacter baumannii - metabolism Animals Anti-Bacterial Agents - metabolism Anti-Bacterial Agents - pharmacology Anti-infective activity Anti-Infective Agents - metabolism Antivirulence activity Bacterial Proteins - genetics Bacterial Proteins - metabolism Biofilms Immunity Proteomics Pyrogallol - metabolism Pyrogallol - pharmacology Quantitative proteomics Tandem Mass Spectrometry Virulence Zebrafish - metabolism |
| title | Pyrogallol downregulates the expression of virulence-associated proteins in Acinetobacter baumannii and showing anti-infection activity by improving non-specific immune response in zebrafish model |
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