Delayed hair cycle in mnd2 mutant mice lacking HtrA2 serine protease activity

The premature death and degeneration of striatal neurons are typical hallmarks of HtrA2-inactivated motor neuron degeneration 2 (mnd2) mice. Although HtrA2 has been extensively studied in relation to the regulation of apoptosis using mnd2 mice, little is known about the other physiological functions...

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Veröffentlicht in:Biochemical and biophysical research communications 2023-01, Vol.641, p.102-109
Hauptverfasser: Nam, Min-Kyung, Shin, Hyun-Ah, Yun, HeeJae, Yoo, Seung-Ah, Rhim, Hyangshuk
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container_title Biochemical and biophysical research communications
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creator Nam, Min-Kyung
Shin, Hyun-Ah
Yun, HeeJae
Yoo, Seung-Ah
Rhim, Hyangshuk
description The premature death and degeneration of striatal neurons are typical hallmarks of HtrA2-inactivated motor neuron degeneration 2 (mnd2) mice. Although HtrA2 has been extensively studied in relation to the regulation of apoptosis using mnd2 mice, little is known about the other physiological functions of HtrA2. In this study, we found that the skin color of wild-type (WT) and mnd2 mice was black and pink on postnatal day 32. Using histological and molecular assays (i.e., assessing the activation of MAPK and expression patterns of PCNA), we demonstrated that this differential skin color change is consistent with the delay in the telogen − to − anagen phase of the hair cycle in mnd2 mice. We also examined adipocytes in the subcutaneous skin layer, finding that HtrA2 inactivation leads to the growth retardation of adipocytes, thereby delaying the hair cycle of mnd2 mice. Collectively, these findings show for the first time that HtrA2 plays an essential role in regulating the adipogenesis-associated hair cycle. [Display omitted] •The hair cycle is delayed in mnd2 mice.•Delayed hair cycle of mnd2 is verified by MAPK activation and PCNA level.•The thickness of the subcutaneous layer is thinned in mnd2 mice.•Increase of the genes involved in adipocyte maturation is delayed in mnd2 mice.•The activity of HtrA2 is linked to regulating the adipogenesis-associated hair cycle.
doi_str_mv 10.1016/j.bbrc.2022.11.034
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[Display omitted] •The hair cycle is delayed in mnd2 mice.•Delayed hair cycle of mnd2 is verified by MAPK activation and PCNA level.•The thickness of the subcutaneous layer is thinned in mnd2 mice.•Increase of the genes involved in adipocyte maturation is delayed in mnd2 mice.•The activity of HtrA2 is linked to regulating the adipogenesis-associated hair cycle.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2022.11.034</identifier><identifier>PMID: 36525924</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adipocyte differentiation ; Animals ; Apoptosis ; Hair - metabolism ; Hair follicle ; High-Temperature Requirement A Serine Peptidase 2 - genetics ; HtrA2 ; Mice ; Mitochondrial Proteins - metabolism ; Serine Endopeptidases - genetics ; Serine Endopeptidases - metabolism ; Serine protease</subject><ispartof>Biochemical and biophysical research communications, 2023-01, Vol.641, p.102-109</ispartof><rights>2022 The Authors</rights><rights>Copyright © 2022 The Authors. 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subjects Adipocyte differentiation
Animals
Apoptosis
Hair - metabolism
Hair follicle
High-Temperature Requirement A Serine Peptidase 2 - genetics
HtrA2
Mice
Mitochondrial Proteins - metabolism
Serine Endopeptidases - genetics
Serine Endopeptidases - metabolism
Serine protease
title Delayed hair cycle in mnd2 mutant mice lacking HtrA2 serine protease activity
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