Electrochemical immunosensor based on AuNPs/ERGO@CNT nanocomposites by one-step electrochemical co-reduction for sensitive detection of P-glycoprotein in serum
P-glycoprotein (P-gp), a transmembrane glycoprotein widely expressed on the surface of various cells, is highly associated with multidrug resistance (MDR) that heralds the malignant progress of disease after drug treatment. Notably, there have been reported that serum P-gp is a potential marker for...
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| Veröffentlicht in: | Biosensors & bioelectronics 2023-02, Vol.222, p.115001-115001, Article 115001 |
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| container_title | Biosensors & bioelectronics |
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| creator | Lu, Tai-Cheng Sun, Yi-Ming Zhong, Yu Lin, Xin-Hua Lei, Yun Liu, Ai-Lin |
| description | P-glycoprotein (P-gp), a transmembrane glycoprotein widely expressed on the surface of various cells, is highly associated with multidrug resistance (MDR) that heralds the malignant progress of disease after drug treatment. Notably, there have been reported that serum P-gp is a potential marker for assessing the progression of disease resistance. Currently, there are few methods for point-of-care serum P-gp detection. In this study, we proposed a gold nanoparticles/electrochemically reduced graphene oxide@carbon nanotube (AuNPs/ERGO@CNT) modified immunosensor based on a one-step electrochemical co-reduction method. The limit of detection (LOD) of our constructed electrochemical immunosensor for P-gp detection reached 0.13 ng/mL, and the detection results in serum were consistent with ELISA. The developed immunosensor is expected to provide a scientific basis for the clinical application of serum P-gp monitoring and integrated medicine. |
| doi_str_mv | 10.1016/j.bios.2022.115001 |
| format | Article |
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Notably, there have been reported that serum P-gp is a potential marker for assessing the progression of disease resistance. Currently, there are few methods for point-of-care serum P-gp detection. In this study, we proposed a gold nanoparticles/electrochemically reduced graphene oxide@carbon nanotube (AuNPs/ERGO@CNT) modified immunosensor based on a one-step electrochemical co-reduction method. The limit of detection (LOD) of our constructed electrochemical immunosensor for P-gp detection reached 0.13 ng/mL, and the detection results in serum were consistent with ELISA. The developed immunosensor is expected to provide a scientific basis for the clinical application of serum P-gp monitoring and integrated medicine.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2022.115001</identifier><identifier>PMID: 36516634</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>ATP Binding Cassette Transporter, Subfamily B ; ATP Binding Cassette Transporter, Subfamily B, Member 1 ; Biosensing Techniques - methods ; Carbon nanotubes ; Electrochemical immunosensor ; Electrochemical Techniques - methods ; Gold ; Gold nanoparticles ; Graphene oxide ; Graphite ; Immunoassay - methods ; Limit of Detection ; Metal Nanoparticles ; Nanocomposites ; P-glycoprotein</subject><ispartof>Biosensors & bioelectronics, 2023-02, Vol.222, p.115001-115001, Article 115001</ispartof><rights>2022 Elsevier B.V.</rights><rights>Copyright © 2022 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-bc79b8e67ac66f9f434fb1948d9f17c4eecdaceae473b1ef4504634b88b832a23</citedby><cites>FETCH-LOGICAL-c356t-bc79b8e67ac66f9f434fb1948d9f17c4eecdaceae473b1ef4504634b88b832a23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0956566322010417$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36516634$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lu, Tai-Cheng</creatorcontrib><creatorcontrib>Sun, Yi-Ming</creatorcontrib><creatorcontrib>Zhong, Yu</creatorcontrib><creatorcontrib>Lin, Xin-Hua</creatorcontrib><creatorcontrib>Lei, Yun</creatorcontrib><creatorcontrib>Liu, Ai-Lin</creatorcontrib><title>Electrochemical immunosensor based on AuNPs/ERGO@CNT nanocomposites by one-step electrochemical co-reduction for sensitive detection of P-glycoprotein in serum</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>P-glycoprotein (P-gp), a transmembrane glycoprotein widely expressed on the surface of various cells, is highly associated with multidrug resistance (MDR) that heralds the malignant progress of disease after drug treatment. 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The developed immunosensor is expected to provide a scientific basis for the clinical application of serum P-gp monitoring and integrated medicine.</description><subject>ATP Binding Cassette Transporter, Subfamily B</subject><subject>ATP Binding Cassette Transporter, Subfamily B, Member 1</subject><subject>Biosensing Techniques - methods</subject><subject>Carbon nanotubes</subject><subject>Electrochemical immunosensor</subject><subject>Electrochemical Techniques - methods</subject><subject>Gold</subject><subject>Gold nanoparticles</subject><subject>Graphene oxide</subject><subject>Graphite</subject><subject>Immunoassay - methods</subject><subject>Limit of Detection</subject><subject>Metal Nanoparticles</subject><subject>Nanocomposites</subject><subject>P-glycoprotein</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9q3DAQh0VpaLZJX6CHomMv3uifZRt6aFi2aSAkIaRnIcmjVottbSU5sE_TV60Wpzn0EBgQDN98zOiH0EdK1pRQebFbGx_SmhHG1pTWhNA3aEXbhleC8fotWpGullUtJT9F71PaEUIa2pF36JTLmpa2WKE_2wFsjsH-gtFbPWA_jvMUEkwpRGx0gh6HCV_Ot_fpYvtwdfd1c_uIJz0FG8Z9SD5DwuZQGKhShj2G_3w2VBH62WZfNK44j2qf_RPgHjIs_eDwffVzONiwjyGDn3CpBHEez9GJ00OCD8_vGfrxbfu4-V7d3F1dby5vKstrmStjm860IBttpXSdE1w4QzvR9p2jjRUAttcWNIiGGwpO1ESU-03bmpYzzfgZ-rx4ywK_Z0hZjT5ZGAY9QZiTYk0tWkkJ4wVlC2pjSCmCU_voRx0PihJ1DEbt1DEYdQxGLcGUoU_P_tmM0L-M_EuiAF8WAMqVTx6iStbDZKH3sfyS6oN_zf8XaoWjDg</recordid><startdate>20230215</startdate><enddate>20230215</enddate><creator>Lu, Tai-Cheng</creator><creator>Sun, Yi-Ming</creator><creator>Zhong, Yu</creator><creator>Lin, Xin-Hua</creator><creator>Lei, Yun</creator><creator>Liu, Ai-Lin</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20230215</creationdate><title>Electrochemical immunosensor based on AuNPs/ERGO@CNT nanocomposites by one-step electrochemical co-reduction for sensitive detection of P-glycoprotein in serum</title><author>Lu, Tai-Cheng ; Sun, Yi-Ming ; Zhong, Yu ; Lin, Xin-Hua ; Lei, Yun ; Liu, Ai-Lin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-bc79b8e67ac66f9f434fb1948d9f17c4eecdaceae473b1ef4504634b88b832a23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>ATP Binding Cassette Transporter, Subfamily B</topic><topic>ATP Binding Cassette Transporter, Subfamily B, Member 1</topic><topic>Biosensing Techniques - methods</topic><topic>Carbon nanotubes</topic><topic>Electrochemical immunosensor</topic><topic>Electrochemical Techniques - methods</topic><topic>Gold</topic><topic>Gold nanoparticles</topic><topic>Graphene oxide</topic><topic>Graphite</topic><topic>Immunoassay - methods</topic><topic>Limit of Detection</topic><topic>Metal Nanoparticles</topic><topic>Nanocomposites</topic><topic>P-glycoprotein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Tai-Cheng</creatorcontrib><creatorcontrib>Sun, Yi-Ming</creatorcontrib><creatorcontrib>Zhong, Yu</creatorcontrib><creatorcontrib>Lin, Xin-Hua</creatorcontrib><creatorcontrib>Lei, Yun</creatorcontrib><creatorcontrib>Liu, Ai-Lin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Tai-Cheng</au><au>Sun, Yi-Ming</au><au>Zhong, Yu</au><au>Lin, Xin-Hua</au><au>Lei, Yun</au><au>Liu, Ai-Lin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrochemical immunosensor based on AuNPs/ERGO@CNT nanocomposites by one-step electrochemical co-reduction for sensitive detection of P-glycoprotein in serum</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2023-02-15</date><risdate>2023</risdate><volume>222</volume><spage>115001</spage><epage>115001</epage><pages>115001-115001</pages><artnum>115001</artnum><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>P-glycoprotein (P-gp), a transmembrane glycoprotein widely expressed on the surface of various cells, is highly associated with multidrug resistance (MDR) that heralds the malignant progress of disease after drug treatment. 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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | ATP Binding Cassette Transporter, Subfamily B ATP Binding Cassette Transporter, Subfamily B, Member 1 Biosensing Techniques - methods Carbon nanotubes Electrochemical immunosensor Electrochemical Techniques - methods Gold Gold nanoparticles Graphene oxide Graphite Immunoassay - methods Limit of Detection Metal Nanoparticles Nanocomposites P-glycoprotein |
| title | Electrochemical immunosensor based on AuNPs/ERGO@CNT nanocomposites by one-step electrochemical co-reduction for sensitive detection of P-glycoprotein in serum |
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