Fertility results after exocervical insemination using goat semen cryopreserved with extenders based on egg yolk, skim milk, or soybean lecithin
To evaluate the effects of four extenders on the post‐thaw quality and fertility of goat semen, six Yunshang Black bucks' semen was collected, pooled, diluted with Andromed® (Andr®), Optidyl® (Opt®), P3644 Sigma l‐phosphatidylcholine (l‐α SL), and skim milk‐based (Milk) extenders, and then cryo...
Gespeichert in:
| Veröffentlicht in: | Reproduction in domestic animals 2023-03, Vol.58 (3), p.431-442 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 442 |
|---|---|
| container_issue | 3 |
| container_start_page | 431 |
| container_title | Reproduction in domestic animals |
| container_volume | 58 |
| creator | Liang, Jiachong Larbi, Allai Lv, Chunrong Ali, Sikandar Wu, Guoquan Quan, Guobo |
| description | To evaluate the effects of four extenders on the post‐thaw quality and fertility of goat semen, six Yunshang Black bucks' semen was collected, pooled, diluted with Andromed® (Andr®), Optidyl® (Opt®), P3644 Sigma l‐phosphatidylcholine (l‐α SL), and skim milk‐based (Milk) extenders, and then cryopreserved. The sperm motilities, abnormalities, membrane and acrosome integrity, mitochondrial activity, apoptosis, and reactive oxygen species (ROS) production were evaluated after thawing. After exocervical insemination with the thawed semen, the pregnancy, lambing, and twinning rates were recorded and compared. The results showed that sperm motilities, membrane integrity, acrosome integrity, mitochondrial activity, and viable spermatozoa were significantly higher in the Andr® and Opt® groups than those in the l‐α SL and Milk groups (p .05). The sperm abnormality was lower in semen frozen with the Andr® or Opt® extenders, as compared to the l‐α SL or Milk extender (p .05). In conclusion, egg yolk or skim milk can be substituted by soybean lecithin during cryopreservation of goat semen. |
| doi_str_mv | 10.1111/rda.14304 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2754050038</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2754050038</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3534-ab296289ba47b4a9f9a5919840da5d5a065baf6f18c53f1bc828f1df3527d7a63</originalsourceid><addsrcrecordid>eNp1kUFrFTEUhYMo9lld-Ack4EbBaZNJMsksS7VaKAii6yGTuXmmzSTPJNM6_6I_2dTXdiF4N_dy-M7hwkHoNSVHtM5xmvQR5YzwJ2hTd98QwehTtCE965pOduoAvcj5khAqlJTP0QHrBCWSkw26PYNUnHdlxQny4kvG2hZIGH5HA-naGe2xCxlmF3RxMeAlu7DF26gLrioEbNIad9VcaZjwjSs_q7lAmCBlPOpcxWqD7Rav0V99wPnKzXh2d2dMOMd1BB2wB1OdLrxEz6z2GV7d70P04-zT99MvzcXXz-enJxeNYYLxRo9t37WqHzWXI9e97bXoaa84mbSYhCadGLXtLFVGMEtHo1pl6WSZaOUkdccO0bt97i7FXwvkMswuG_BeB4hLHlopOBGEMFXRt_-gl3FJoX5XKUUJ41SKSr3fUybFnBPYYZfcrNM6UDLc1TTUmoa_NVX2zX3iMs4wPZIPvVTgeA_cOA_r_5OGbx9P9pF_ABxinls</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2781034175</pqid></control><display><type>article</type><title>Fertility results after exocervical insemination using goat semen cryopreserved with extenders based on egg yolk, skim milk, or soybean lecithin</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Liang, Jiachong ; Larbi, Allai ; Lv, Chunrong ; Ali, Sikandar ; Wu, Guoquan ; Quan, Guobo</creator><creatorcontrib>Liang, Jiachong ; Larbi, Allai ; Lv, Chunrong ; Ali, Sikandar ; Wu, Guoquan ; Quan, Guobo</creatorcontrib><description>To evaluate the effects of four extenders on the post‐thaw quality and fertility of goat semen, six Yunshang Black bucks' semen was collected, pooled, diluted with Andromed® (Andr®), Optidyl® (Opt®), P3644 Sigma l‐phosphatidylcholine (l‐α SL), and skim milk‐based (Milk) extenders, and then cryopreserved. The sperm motilities, abnormalities, membrane and acrosome integrity, mitochondrial activity, apoptosis, and reactive oxygen species (ROS) production were evaluated after thawing. After exocervical insemination with the thawed semen, the pregnancy, lambing, and twinning rates were recorded and compared. The results showed that sperm motilities, membrane integrity, acrosome integrity, mitochondrial activity, and viable spermatozoa were significantly higher in the Andr® and Opt® groups than those in the l‐α SL and Milk groups (p < .05). Furthermore, there was no difference between Andr® and Opt® (p > .05). The sperm abnormality was lower in semen frozen with the Andr® or Opt® extenders, as compared to the l‐α SL or Milk extender (p < .05). Regarding, the viable cells with low ROS production, the optimal results were obtained in the semen frozen with Andr® and Opt® extenders. Following exocervical insemination, the pregnancy and lambing rates in the Milk group were significantly lower than those in the other groups (p < .05). No difference was found in the pregnancy and lambing rates between Andr®, Opt®, and l‐α SL (p > .05). Furthermore, the twinning rates were similar between these four groups (p > .05). In conclusion, egg yolk or skim milk can be substituted by soybean lecithin during cryopreservation of goat semen.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.14304</identifier><identifier>PMID: 36510740</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Abnormalities ; Animals ; Apoptosis ; artificial insemination ; Cryopreservation ; Cryopreservation - methods ; Cryopreservation - veterinary ; Cryoprotective Agents - pharmacology ; Egg Yolk ; Eggs ; Female ; Fertility ; Glycine max ; goat semen ; Goats ; Insemination, Artificial - veterinary ; Integrity ; Lecithin ; Lecithins - pharmacology ; Male ; Membranes ; Milk ; Mitochondria ; Phosphatidylcholine ; Pregnancy ; Reactive Oxygen Species ; Seeds ; Semen ; Semen Preservation - methods ; Semen Preservation - veterinary ; Sheep ; Skim milk ; soybean lecithin ; Soybeans ; Sperm ; Spermatozoa ; Thawing ; Yolk</subject><ispartof>Reproduction in domestic animals, 2023-03, Vol.58 (3), p.431-442</ispartof><rights>2022 Wiley‐VCH GmbH.</rights><rights>2022 Wiley-VCH GmbH.</rights><rights>Copyright © 2023 Wiley‐VCH GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3534-ab296289ba47b4a9f9a5919840da5d5a065baf6f18c53f1bc828f1df3527d7a63</citedby><cites>FETCH-LOGICAL-c3534-ab296289ba47b4a9f9a5919840da5d5a065baf6f18c53f1bc828f1df3527d7a63</cites><orcidid>0000-0002-8167-3623 ; 0000-0002-4727-6990</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Frda.14304$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Frda.14304$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36510740$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liang, Jiachong</creatorcontrib><creatorcontrib>Larbi, Allai</creatorcontrib><creatorcontrib>Lv, Chunrong</creatorcontrib><creatorcontrib>Ali, Sikandar</creatorcontrib><creatorcontrib>Wu, Guoquan</creatorcontrib><creatorcontrib>Quan, Guobo</creatorcontrib><title>Fertility results after exocervical insemination using goat semen cryopreserved with extenders based on egg yolk, skim milk, or soybean lecithin</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>To evaluate the effects of four extenders on the post‐thaw quality and fertility of goat semen, six Yunshang Black bucks' semen was collected, pooled, diluted with Andromed® (Andr®), Optidyl® (Opt®), P3644 Sigma l‐phosphatidylcholine (l‐α SL), and skim milk‐based (Milk) extenders, and then cryopreserved. The sperm motilities, abnormalities, membrane and acrosome integrity, mitochondrial activity, apoptosis, and reactive oxygen species (ROS) production were evaluated after thawing. After exocervical insemination with the thawed semen, the pregnancy, lambing, and twinning rates were recorded and compared. The results showed that sperm motilities, membrane integrity, acrosome integrity, mitochondrial activity, and viable spermatozoa were significantly higher in the Andr® and Opt® groups than those in the l‐α SL and Milk groups (p < .05). Furthermore, there was no difference between Andr® and Opt® (p > .05). The sperm abnormality was lower in semen frozen with the Andr® or Opt® extenders, as compared to the l‐α SL or Milk extender (p < .05). Regarding, the viable cells with low ROS production, the optimal results were obtained in the semen frozen with Andr® and Opt® extenders. Following exocervical insemination, the pregnancy and lambing rates in the Milk group were significantly lower than those in the other groups (p < .05). No difference was found in the pregnancy and lambing rates between Andr®, Opt®, and l‐α SL (p > .05). Furthermore, the twinning rates were similar between these four groups (p > .05). In conclusion, egg yolk or skim milk can be substituted by soybean lecithin during cryopreservation of goat semen.</description><subject>Abnormalities</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>artificial insemination</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Cryopreservation - veterinary</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Egg Yolk</subject><subject>Eggs</subject><subject>Female</subject><subject>Fertility</subject><subject>Glycine max</subject><subject>goat semen</subject><subject>Goats</subject><subject>Insemination, Artificial - veterinary</subject><subject>Integrity</subject><subject>Lecithin</subject><subject>Lecithins - pharmacology</subject><subject>Male</subject><subject>Membranes</subject><subject>Milk</subject><subject>Mitochondria</subject><subject>Phosphatidylcholine</subject><subject>Pregnancy</subject><subject>Reactive Oxygen Species</subject><subject>Seeds</subject><subject>Semen</subject><subject>Semen Preservation - methods</subject><subject>Semen Preservation - veterinary</subject><subject>Sheep</subject><subject>Skim milk</subject><subject>soybean lecithin</subject><subject>Soybeans</subject><subject>Sperm</subject><subject>Spermatozoa</subject><subject>Thawing</subject><subject>Yolk</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUFrFTEUhYMo9lld-Ack4EbBaZNJMsksS7VaKAii6yGTuXmmzSTPJNM6_6I_2dTXdiF4N_dy-M7hwkHoNSVHtM5xmvQR5YzwJ2hTd98QwehTtCE965pOduoAvcj5khAqlJTP0QHrBCWSkw26PYNUnHdlxQny4kvG2hZIGH5HA-naGe2xCxlmF3RxMeAlu7DF26gLrioEbNIad9VcaZjwjSs_q7lAmCBlPOpcxWqD7Rav0V99wPnKzXh2d2dMOMd1BB2wB1OdLrxEz6z2GV7d70P04-zT99MvzcXXz-enJxeNYYLxRo9t37WqHzWXI9e97bXoaa84mbSYhCadGLXtLFVGMEtHo1pl6WSZaOUkdccO0bt97i7FXwvkMswuG_BeB4hLHlopOBGEMFXRt_-gl3FJoX5XKUUJ41SKSr3fUybFnBPYYZfcrNM6UDLc1TTUmoa_NVX2zX3iMs4wPZIPvVTgeA_cOA_r_5OGbx9P9pF_ABxinls</recordid><startdate>202303</startdate><enddate>202303</enddate><creator>Liang, Jiachong</creator><creator>Larbi, Allai</creator><creator>Lv, Chunrong</creator><creator>Ali, Sikandar</creator><creator>Wu, Guoquan</creator><creator>Quan, Guobo</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8167-3623</orcidid><orcidid>https://orcid.org/0000-0002-4727-6990</orcidid></search><sort><creationdate>202303</creationdate><title>Fertility results after exocervical insemination using goat semen cryopreserved with extenders based on egg yolk, skim milk, or soybean lecithin</title><author>Liang, Jiachong ; Larbi, Allai ; Lv, Chunrong ; Ali, Sikandar ; Wu, Guoquan ; Quan, Guobo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3534-ab296289ba47b4a9f9a5919840da5d5a065baf6f18c53f1bc828f1df3527d7a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Abnormalities</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>artificial insemination</topic><topic>Cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Cryopreservation - veterinary</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Egg Yolk</topic><topic>Eggs</topic><topic>Female</topic><topic>Fertility</topic><topic>Glycine max</topic><topic>goat semen</topic><topic>Goats</topic><topic>Insemination, Artificial - veterinary</topic><topic>Integrity</topic><topic>Lecithin</topic><topic>Lecithins - pharmacology</topic><topic>Male</topic><topic>Membranes</topic><topic>Milk</topic><topic>Mitochondria</topic><topic>Phosphatidylcholine</topic><topic>Pregnancy</topic><topic>Reactive Oxygen Species</topic><topic>Seeds</topic><topic>Semen</topic><topic>Semen Preservation - methods</topic><topic>Semen Preservation - veterinary</topic><topic>Sheep</topic><topic>Skim milk</topic><topic>soybean lecithin</topic><topic>Soybeans</topic><topic>Sperm</topic><topic>Spermatozoa</topic><topic>Thawing</topic><topic>Yolk</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liang, Jiachong</creatorcontrib><creatorcontrib>Larbi, Allai</creatorcontrib><creatorcontrib>Lv, Chunrong</creatorcontrib><creatorcontrib>Ali, Sikandar</creatorcontrib><creatorcontrib>Wu, Guoquan</creatorcontrib><creatorcontrib>Quan, Guobo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liang, Jiachong</au><au>Larbi, Allai</au><au>Lv, Chunrong</au><au>Ali, Sikandar</au><au>Wu, Guoquan</au><au>Quan, Guobo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fertility results after exocervical insemination using goat semen cryopreserved with extenders based on egg yolk, skim milk, or soybean lecithin</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2023-03</date><risdate>2023</risdate><volume>58</volume><issue>3</issue><spage>431</spage><epage>442</epage><pages>431-442</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>To evaluate the effects of four extenders on the post‐thaw quality and fertility of goat semen, six Yunshang Black bucks' semen was collected, pooled, diluted with Andromed® (Andr®), Optidyl® (Opt®), P3644 Sigma l‐phosphatidylcholine (l‐α SL), and skim milk‐based (Milk) extenders, and then cryopreserved. The sperm motilities, abnormalities, membrane and acrosome integrity, mitochondrial activity, apoptosis, and reactive oxygen species (ROS) production were evaluated after thawing. After exocervical insemination with the thawed semen, the pregnancy, lambing, and twinning rates were recorded and compared. The results showed that sperm motilities, membrane integrity, acrosome integrity, mitochondrial activity, and viable spermatozoa were significantly higher in the Andr® and Opt® groups than those in the l‐α SL and Milk groups (p < .05). Furthermore, there was no difference between Andr® and Opt® (p > .05). The sperm abnormality was lower in semen frozen with the Andr® or Opt® extenders, as compared to the l‐α SL or Milk extender (p < .05). Regarding, the viable cells with low ROS production, the optimal results were obtained in the semen frozen with Andr® and Opt® extenders. Following exocervical insemination, the pregnancy and lambing rates in the Milk group were significantly lower than those in the other groups (p < .05). No difference was found in the pregnancy and lambing rates between Andr®, Opt®, and l‐α SL (p > .05). Furthermore, the twinning rates were similar between these four groups (p > .05). In conclusion, egg yolk or skim milk can be substituted by soybean lecithin during cryopreservation of goat semen.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>36510740</pmid><doi>10.1111/rda.14304</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-8167-3623</orcidid><orcidid>https://orcid.org/0000-0002-4727-6990</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0936-6768 |
| ispartof | Reproduction in domestic animals, 2023-03, Vol.58 (3), p.431-442 |
| issn | 0936-6768 1439-0531 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2754050038 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Abnormalities Animals Apoptosis artificial insemination Cryopreservation Cryopreservation - methods Cryopreservation - veterinary Cryoprotective Agents - pharmacology Egg Yolk Eggs Female Fertility Glycine max goat semen Goats Insemination, Artificial - veterinary Integrity Lecithin Lecithins - pharmacology Male Membranes Milk Mitochondria Phosphatidylcholine Pregnancy Reactive Oxygen Species Seeds Semen Semen Preservation - methods Semen Preservation - veterinary Sheep Skim milk soybean lecithin Soybeans Sperm Spermatozoa Thawing Yolk |
| title | Fertility results after exocervical insemination using goat semen cryopreserved with extenders based on egg yolk, skim milk, or soybean lecithin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T16%3A49%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Fertility%20results%20after%20exocervical%20insemination%20using%20goat%20semen%20cryopreserved%20with%20extenders%20based%20on%20egg%20yolk,%20skim%20milk,%20or%20soybean%20lecithin&rft.jtitle=Reproduction%20in%20domestic%20animals&rft.au=Liang,%20Jiachong&rft.date=2023-03&rft.volume=58&rft.issue=3&rft.spage=431&rft.epage=442&rft.pages=431-442&rft.issn=0936-6768&rft.eissn=1439-0531&rft_id=info:doi/10.1111/rda.14304&rft_dat=%3Cproquest_cross%3E2754050038%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2781034175&rft_id=info:pmid/36510740&rfr_iscdi=true |