Fluorometric Protocol for Estimating Peroxiredoxin Activity in Biological Tissues

This protocol describes a detailed fluorometric method for measuring peroxiredoxin (Prx) enzyme activity in vitro. Peroxide dissociation is the rate-limiting step in the Prx-controlled enzymatic reaction. To prevent interference by the catalase enzyme, we developed a peroxiredoxin assay that measure...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of fluorescence 2023-03, Vol.33 (2), p.721-730
Hauptverfasser:	Hussein, Marwah Jaber, Hadwan, Mahmoud Hussein
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical Chemistry Assaying Biochemistry Biological activity Biological and Medical Physics Biomedical and Life Sciences Biomedicine Biophysics Biotechnology Butyl hydroperoxide Catalase Correlation coefficients Design optimization Enzyme activity Enzymes Fluorescence Fluorimetry Measurement methods Reagents Sodium azides Substrates Tissues
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!