Fabrication and biological evaluation of three-dimensional (3D) Mg substituted bi-phasic calcium phosphate porous scaffolds for hard tissue engineering
This work reports on the fabrication of three-dimensional (3D) magnesium substituted bi-phasic calcium phosphate (Mg-BCP) scaffolds by gel-casting, their structural and physico-chemical characterization, and on the assessment of their in vitro and in vivo performances. The crystalline phase assembla...
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| Veröffentlicht in: | RSC advances 2022-11, Vol.12 (52), p.3376-33715 |
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| creator | Ramadas, Munusamy Abimanyu, Ravichandran Ferreira, José M. F Ballamurugan, Anbalagan M |
| description | This work reports on the fabrication of three-dimensional (3D) magnesium substituted bi-phasic calcium phosphate (Mg-BCP) scaffolds by gel-casting, their structural and physico-chemical characterization, and on the assessment of their
in vitro
and
in vivo
performances. The crystalline phase assemblage, chemical functional groups and porous morphology features of the scaffolds were evaluated by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR) and field emission scanning electron microscopy (FE-SEM), respectively. The sintered scaffolds revealed an interconnected porosity with pore sizes ranging from 4.3 to 7.28 μm. The scaffolds exhibited good biomineralization activity upon immersion in simulated body fluid (SBF), while an
in vitro
study using MG-63 cell line cultures confirmed their improved biocompatibility, cell proliferation and bioactivity. Bone grafting of 3D scaffolds was performed in non-load bearing bone defects surgically created in tibia of rabbits, used as animal model. Histological and radiological observations indicated the successful restoration of bone defects. The overall results confirmed the suitability of the scaffolds to be further tested as synthetic bone grafts in bone regeneration surgeries and in bone tissue engineering applications.
This work reports on the fabrication of three-dimensional (3D) magnesium substituted bi-phasic calcium phosphate (Mg-BCP) scaffolds by gel-casting, their structural and physico-chemical characterization, and on the assessment of their
in vitro
and
in vivo
performances. |
| doi_str_mv | 10.1039/d2ra04009c |
| format | Article |
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in vitro
and
in vivo
performances. The crystalline phase assemblage, chemical functional groups and porous morphology features of the scaffolds were evaluated by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR) and field emission scanning electron microscopy (FE-SEM), respectively. The sintered scaffolds revealed an interconnected porosity with pore sizes ranging from 4.3 to 7.28 μm. The scaffolds exhibited good biomineralization activity upon immersion in simulated body fluid (SBF), while an
in vitro
study using MG-63 cell line cultures confirmed their improved biocompatibility, cell proliferation and bioactivity. Bone grafting of 3D scaffolds was performed in non-load bearing bone defects surgically created in tibia of rabbits, used as animal model. Histological and radiological observations indicated the successful restoration of bone defects. The overall results confirmed the suitability of the scaffolds to be further tested as synthetic bone grafts in bone regeneration surgeries and in bone tissue engineering applications.
This work reports on the fabrication of three-dimensional (3D) magnesium substituted bi-phasic calcium phosphate (Mg-BCP) scaffolds by gel-casting, their structural and physico-chemical characterization, and on the assessment of their
in vitro
and
in vivo
performances.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/d2ra04009c</identifier><identifier>PMID: 36505699</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Biocompatibility ; Biomedical materials ; Body fluids ; Calcium phosphates ; Chemistry ; Crystal defects ; Evaluation ; Field emission microscopy ; Field emission spectroscopy ; Fourier transforms ; Functional groups ; Gelcasting ; In vitro methods and tests ; Infrared spectroscopy ; Magnesium ; Phase assemblages ; Rabbits ; Regeneration (physiology) ; Scaffolds ; Sintering (powder metallurgy) ; Structural analysis ; Substitute bone ; Tibia ; Tissue engineering</subject><ispartof>RSC advances, 2022-11, Vol.12 (52), p.3376-33715</ispartof><rights>This journal is © The Royal Society of Chemistry.</rights><rights>Copyright Royal Society of Chemistry 2022</rights><rights>This journal is © The Royal Society of Chemistry 2022 The Royal Society of Chemistry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c387t-a7757a51d11a520a63501d1d54fce48c144ff4bdd97e93f98281fb45688ce2163</cites><orcidid>0000-0002-5059-587X ; 0000-0002-7520-2809</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9685373/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9685373/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36505699$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramadas, Munusamy</creatorcontrib><creatorcontrib>Abimanyu, Ravichandran</creatorcontrib><creatorcontrib>Ferreira, José M. F</creatorcontrib><creatorcontrib>Ballamurugan, Anbalagan M</creatorcontrib><title>Fabrication and biological evaluation of three-dimensional (3D) Mg substituted bi-phasic calcium phosphate porous scaffolds for hard tissue engineering</title><title>RSC advances</title><addtitle>RSC Adv</addtitle><description>This work reports on the fabrication of three-dimensional (3D) magnesium substituted bi-phasic calcium phosphate (Mg-BCP) scaffolds by gel-casting, their structural and physico-chemical characterization, and on the assessment of their
in vitro
and
in vivo
performances. The crystalline phase assemblage, chemical functional groups and porous morphology features of the scaffolds were evaluated by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR) and field emission scanning electron microscopy (FE-SEM), respectively. The sintered scaffolds revealed an interconnected porosity with pore sizes ranging from 4.3 to 7.28 μm. The scaffolds exhibited good biomineralization activity upon immersion in simulated body fluid (SBF), while an
in vitro
study using MG-63 cell line cultures confirmed their improved biocompatibility, cell proliferation and bioactivity. Bone grafting of 3D scaffolds was performed in non-load bearing bone defects surgically created in tibia of rabbits, used as animal model. Histological and radiological observations indicated the successful restoration of bone defects. The overall results confirmed the suitability of the scaffolds to be further tested as synthetic bone grafts in bone regeneration surgeries and in bone tissue engineering applications.
This work reports on the fabrication of three-dimensional (3D) magnesium substituted bi-phasic calcium phosphate (Mg-BCP) scaffolds by gel-casting, their structural and physico-chemical characterization, and on the assessment of their
in vitro
and
in vivo
performances.</description><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Body fluids</subject><subject>Calcium phosphates</subject><subject>Chemistry</subject><subject>Crystal defects</subject><subject>Evaluation</subject><subject>Field emission microscopy</subject><subject>Field emission spectroscopy</subject><subject>Fourier transforms</subject><subject>Functional groups</subject><subject>Gelcasting</subject><subject>In vitro methods and tests</subject><subject>Infrared spectroscopy</subject><subject>Magnesium</subject><subject>Phase assemblages</subject><subject>Rabbits</subject><subject>Regeneration (physiology)</subject><subject>Scaffolds</subject><subject>Sintering (powder metallurgy)</subject><subject>Structural analysis</subject><subject>Substitute bone</subject><subject>Tibia</subject><subject>Tissue engineering</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpdkt1r1TAYxosobszdeK8EvNmEzny3vRHG2YfCRBC9Lmnyps1om5qkA_8S_11zPPNsLjdJ3ueXhzd5UhSvCT4jmDUfDA0Kc4wb_aw4pJjLkmLZPH-0PiiOY7zFeUhBqCQviwMmBRayaQ6L31eqC06r5PyM1GxQ5_zo-1wZEdypcd0p3qI0BIDSuAnmmEtZP2EXp-hLj-LaxeTSmmB7vFwGFZ1G2UG7dULL4GMuJUCLD36NKGplrR9NRNYHNKhgUHIxroBg7t0MENzcvypeWDVGOL6fj4ofV5ffN5_Km6_XnzfnN6VmdZVKVVWiUoIYQpSgWEkmcN4Ywa0GXmvCubW8M6apoGG2qWlNbMeFrGsNlEh2VHzc-S5rN4HRMKegxnYJblLhV-uVa_9XZje0vb9rG1kLVrFscHJvEPzPFWJqJxc1jKOaId-2pZVgUnKJcUbfPUFv_RryS24pThihVc0z9X5H6eBjDGD3zRDcbiNvL-i387-RbzL89nH7e_RfwBl4swNC1Hv14c-wPwRhs1U</recordid><startdate>20221122</startdate><enddate>20221122</enddate><creator>Ramadas, Munusamy</creator><creator>Abimanyu, Ravichandran</creator><creator>Ferreira, José M. F</creator><creator>Ballamurugan, Anbalagan M</creator><general>Royal Society of Chemistry</general><general>The Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-5059-587X</orcidid><orcidid>https://orcid.org/0000-0002-7520-2809</orcidid></search><sort><creationdate>20221122</creationdate><title>Fabrication and biological evaluation of three-dimensional (3D) Mg substituted bi-phasic calcium phosphate porous scaffolds for hard tissue engineering</title><author>Ramadas, Munusamy ; Abimanyu, Ravichandran ; Ferreira, José M. F ; Ballamurugan, Anbalagan M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-a7757a51d11a520a63501d1d54fce48c144ff4bdd97e93f98281fb45688ce2163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biocompatibility</topic><topic>Biomedical materials</topic><topic>Body fluids</topic><topic>Calcium phosphates</topic><topic>Chemistry</topic><topic>Crystal defects</topic><topic>Evaluation</topic><topic>Field emission microscopy</topic><topic>Field emission spectroscopy</topic><topic>Fourier transforms</topic><topic>Functional groups</topic><topic>Gelcasting</topic><topic>In vitro methods and tests</topic><topic>Infrared spectroscopy</topic><topic>Magnesium</topic><topic>Phase assemblages</topic><topic>Rabbits</topic><topic>Regeneration (physiology)</topic><topic>Scaffolds</topic><topic>Sintering (powder metallurgy)</topic><topic>Structural analysis</topic><topic>Substitute bone</topic><topic>Tibia</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramadas, Munusamy</creatorcontrib><creatorcontrib>Abimanyu, Ravichandran</creatorcontrib><creatorcontrib>Ferreira, José M. F</creatorcontrib><creatorcontrib>Ballamurugan, Anbalagan M</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramadas, Munusamy</au><au>Abimanyu, Ravichandran</au><au>Ferreira, José M. F</au><au>Ballamurugan, Anbalagan M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fabrication and biological evaluation of three-dimensional (3D) Mg substituted bi-phasic calcium phosphate porous scaffolds for hard tissue engineering</atitle><jtitle>RSC advances</jtitle><addtitle>RSC Adv</addtitle><date>2022-11-22</date><risdate>2022</risdate><volume>12</volume><issue>52</issue><spage>3376</spage><epage>33715</epage><pages>3376-33715</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>This work reports on the fabrication of three-dimensional (3D) magnesium substituted bi-phasic calcium phosphate (Mg-BCP) scaffolds by gel-casting, their structural and physico-chemical characterization, and on the assessment of their
in vitro
and
in vivo
performances. The crystalline phase assemblage, chemical functional groups and porous morphology features of the scaffolds were evaluated by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR) and field emission scanning electron microscopy (FE-SEM), respectively. The sintered scaffolds revealed an interconnected porosity with pore sizes ranging from 4.3 to 7.28 μm. The scaffolds exhibited good biomineralization activity upon immersion in simulated body fluid (SBF), while an
in vitro
study using MG-63 cell line cultures confirmed their improved biocompatibility, cell proliferation and bioactivity. Bone grafting of 3D scaffolds was performed in non-load bearing bone defects surgically created in tibia of rabbits, used as animal model. Histological and radiological observations indicated the successful restoration of bone defects. The overall results confirmed the suitability of the scaffolds to be further tested as synthetic bone grafts in bone regeneration surgeries and in bone tissue engineering applications.
This work reports on the fabrication of three-dimensional (3D) magnesium substituted bi-phasic calcium phosphate (Mg-BCP) scaffolds by gel-casting, their structural and physico-chemical characterization, and on the assessment of their
in vitro
and
in vivo
performances.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>36505699</pmid><doi>10.1039/d2ra04009c</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-5059-587X</orcidid><orcidid>https://orcid.org/0000-0002-7520-2809</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Biocompatibility Biomedical materials Body fluids Calcium phosphates Chemistry Crystal defects Evaluation Field emission microscopy Field emission spectroscopy Fourier transforms Functional groups Gelcasting In vitro methods and tests Infrared spectroscopy Magnesium Phase assemblages Rabbits Regeneration (physiology) Scaffolds Sintering (powder metallurgy) Structural analysis Substitute bone Tibia Tissue engineering |
| title | Fabrication and biological evaluation of three-dimensional (3D) Mg substituted bi-phasic calcium phosphate porous scaffolds for hard tissue engineering |
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