A simple LC-MS/MS method for the simultaneous quantification of drug metabolic enzymes

•A targeted proteomics method to quantify the abundance of drug-related metabolizing enzymes by UPLC-MS/MS.•Ethylene glycol is used to enhance the mass spectral response of surrogate peptides.•Needle washing method and needle washing solution are optimized to avoid serious crossover. The aim of this...

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Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2023-01, Vol.1214, p.123536-123536, Article 123536
Hauptverfasser:	Guo, Xuan, Zhang, Lei, Lei, Zihan, Hou, Zhe, Li, Hui, Li, Xiaodong, Dong, Jing, Song, Ling, Chen, Dingding, Liu, Dongyang
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, Liquid - methods CYP450 enzymes Cytochrome P-450 CYP3A - metabolism Cytochrome P-450 Enzyme System - metabolism Humans LC-MS/MS Microsomes, Liver - metabolism Proteomics Tandem Mass Spectrometry - methods
Online-Zugang:	Volltext
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