Lipase-catalysed synthesis of natural ethanol esters: effect of water removal on enzyme reutilisation
Lipase‐catalysed synthesis of ethanol esters using natural substrates is presented. Initial substrate concentrations, optimised through an experimental design, were 0.8 mol dm−3 lauric acid and 0.58 mol dm−3 ethanol, with an initial esterification rate (r0) of 17.13 mmol dm−3 min−1. Two different wa...
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| Veröffentlicht in: | Journal of chemical technology and biotechnology (1986) 2000-11, Vol.75 (11), p.991-996 |
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| container_issue | 11 |
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| container_title | Journal of chemical technology and biotechnology (1986) |
| container_volume | 75 |
| creator | Río, José L Del Caminal, Gloria Fité, Mercé Faus, Ignacio Bladé, Joan Solà, Carles |
| description | Lipase‐catalysed synthesis of ethanol esters using natural substrates is presented. Initial substrate concentrations, optimised through an experimental design, were 0.8 mol dm−3 lauric acid and 0.58 mol dm−3 ethanol, with an initial esterification rate (r0) of 17.13 mmol dm−3 min−1. Two different water removal systems were compared: a cooling exchanger in the reactor headspace and a vacuum‐based system. The best results were obtained with the second system. After six consecutive operations with the same enzyme, no loss of activity was observed. The yields obtained in all the runs were greater than 90%. Using the same approach three different natural esters (ethyl laureate, ethyl myristate and ethyl palmitate) were synthesised at 0.9 dm3 scale. Similar results were obtained in all three cases, with chemical yields always being greater than 90%.
© 2000 Society of Chemical Industry |
| doi_str_mv | 10.1002/1097-4660(200011)75:11<991::AID-JCTB322>3.0.CO;2-Q |
| format | Article |
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© 2000 Society of Chemical Industry</description><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>catalyst reusability</subject><subject>ethanol ester synthesis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>lipase</subject><subject>Methods. Procedures. Technologies</subject><subject>natural esters</subject><subject>water removal</subject><issn>0268-2575</issn><issn>1097-4660</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqVkV1v0zAUhiMEEmXwH3KBEFyk-Cu2E9CkEVg3VFGNDXp55LonmiFNip2yZb9-rlLGDTfc-EivHj_Hep0kJSVTSgh7S0mhMiElec0IIZS-UXlJ6fuioGV5cv4x-1xdfeCMHfMpmVaLdyy7eJRMHi49TiaESZ2xXOVPk2ch_IgSqZmcJDh3WxMws6Y3zRBwnYah7a8xuJB2ddqafudNk2J_bdouztCjD2WKdY223xM3Jiapx033O3Jdm2J7N2wwJrveNS6Y3nXt8-RJbZqALw7zKPl2-umqOsvmi9l5dTLPrBCcZVRYY7XiRktDpeBSFbYodK5VsdYrIYxUa76iWqNggiMKZaVeSc4iqFdr5EfJq9G79d2vXXwsbFyw2DSmxW4XgKmcc6aKCH4dQeu7EDzWsPVuY_wAlMC-cdiXB_vyYGwcVA7xjI0DxMbh0DhwIFAtgMFFlL48bDfBmqb2prUu_DXnnGiuI_Z9xG5cg8P_LP733j9RFGej2MVfun0QG_8TpOJRs_wyg2p5tryklzOY8Xve367G</recordid><startdate>200011</startdate><enddate>200011</enddate><creator>Río, José L Del</creator><creator>Caminal, Gloria</creator><creator>Fité, Mercé</creator><creator>Faus, Ignacio</creator><creator>Bladé, Joan</creator><creator>Solà, Carles</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope></search><sort><creationdate>200011</creationdate><title>Lipase-catalysed synthesis of natural ethanol esters: effect of water removal on enzyme reutilisation</title><author>Río, José L Del ; Caminal, Gloria ; Fité, Mercé ; Faus, Ignacio ; Bladé, Joan ; Solà, Carles</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4432-14cac873a86a1643679c9985879d8b44a67d3b188e4243ee47c68b6326798bde3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Bioconversions. Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>catalyst reusability</topic><topic>ethanol ester synthesis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>lipase</topic><topic>Methods. Procedures. Technologies</topic><topic>natural esters</topic><topic>water removal</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Río, José L Del</creatorcontrib><creatorcontrib>Caminal, Gloria</creatorcontrib><creatorcontrib>Fité, Mercé</creatorcontrib><creatorcontrib>Faus, Ignacio</creatorcontrib><creatorcontrib>Bladé, Joan</creatorcontrib><creatorcontrib>Solà, Carles</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Río, José L Del</au><au>Caminal, Gloria</au><au>Fité, Mercé</au><au>Faus, Ignacio</au><au>Bladé, Joan</au><au>Solà, Carles</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lipase-catalysed synthesis of natural ethanol esters: effect of water removal on enzyme reutilisation</atitle><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle><addtitle>J. Chem. Technol. Biotechnol</addtitle><date>2000-11</date><risdate>2000</risdate><volume>75</volume><issue>11</issue><spage>991</spage><epage>996</epage><pages>991-996</pages><issn>0268-2575</issn><eissn>1097-4660</eissn><coden>JCTBDC</coden><abstract>Lipase‐catalysed synthesis of ethanol esters using natural substrates is presented. Initial substrate concentrations, optimised through an experimental design, were 0.8 mol dm−3 lauric acid and 0.58 mol dm−3 ethanol, with an initial esterification rate (r0) of 17.13 mmol dm−3 min−1. Two different water removal systems were compared: a cooling exchanger in the reactor headspace and a vacuum‐based system. The best results were obtained with the second system. After six consecutive operations with the same enzyme, no loss of activity was observed. The yields obtained in all the runs were greater than 90%. Using the same approach three different natural esters (ethyl laureate, ethyl myristate and ethyl palmitate) were synthesised at 0.9 dm3 scale. Similar results were obtained in all three cases, with chemical yields always being greater than 90%.
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| ispartof | Journal of chemical technology and biotechnology (1986), 2000-11, Vol.75 (11), p.991-996 |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology catalyst reusability ethanol ester synthesis Fundamental and applied biological sciences. Psychology lipase Methods. Procedures. Technologies natural esters water removal |
| title | Lipase-catalysed synthesis of natural ethanol esters: effect of water removal on enzyme reutilisation |
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