Detection of digitalis compounds using a surface plasmon resonance-based biosensor
An automated surface plasmon resonance-based biosensor system has been used to detect endogenous and exogenous digitalis-like factors (EDLF) in the pmolar range in real time. EDLF was purified from umbilical cord blood. EDLF has been suggested to play a role in hypertension and in perinatal adaptati...
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| Veröffentlicht in: | Biosensors & bioelectronics 1998-11, Vol.13 (10), p.1055-1060 |
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| container_title | Biosensors & bioelectronics |
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| creator | Laricchia-Robbio, Leopoldo Balzan, Silvana Ghione, Sergio Montali, Umberto Revoltella, Roberto P |
| description | An automated surface plasmon resonance-based biosensor system has been used to detect endogenous and exogenous digitalis-like factors (EDLF) in the pmolar range in real time. EDLF was purified from umbilical cord blood. EDLF has been suggested to play a role in hypertension and in perinatal adaptation. Highly specific polyclonal anti-ouabain antibodies showed a high affinity binding capacity for ouabain, ouabagenin and strophantidin with an IC
50 value of 5×10
−10 M, 7.0×10
−10 M and 2×10
−8 M, respectively. EDLF cross-reacted with antibodies and its concentration in plasma at IC
50 was around 50 pmol ouabain equivalent. This study shows the potential usefulness of the biosensor technology for biomolecular interaction analysis. The features of this technology (fully automated, measured in real time, sharpened response) offer several advantages compared with a traditional immunoassay like radioimmunoassay (RIA) in the detection of digitalis compounds in human fluids. |
| doi_str_mv | 10.1016/S0956-5663(98)00065-7 |
| format | Article |
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50 value of 5×10
−10 M, 7.0×10
−10 M and 2×10
−8 M, respectively. EDLF cross-reacted with antibodies and its concentration in plasma at IC
50 was around 50 pmol ouabain equivalent. This study shows the potential usefulness of the biosensor technology for biomolecular interaction analysis. The features of this technology (fully automated, measured in real time, sharpened response) offer several advantages compared with a traditional immunoassay like radioimmunoassay (RIA) in the detection of digitalis compounds in human fluids.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/S0956-5663(98)00065-7</identifier><identifier>PMID: 9842700</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Analytical biochemistry: general aspects, technics, instrumentation ; Analytical, structural and metabolic biochemistry ; Antibodies - blood ; antiouabain antibodies ; BIA technology ; Biological and medical sciences ; Biosensors ; Biotechnology ; Cardenolides ; Digoxin ; endogenous digitalis-like factor ; Enzyme Inhibitors - analysis ; Fundamental and applied biological sciences. Psychology ; Humans ; Infant, Newborn ; Methods. Procedures. Technologies ; Ouabain - immunology ; Radioimmunoassay ; RIA ; Saponins - analysis ; Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors ; Surface Plasmon Resonance ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 1998-11, Vol.13 (10), p.1055-1060</ispartof><rights>1998 Elsevier Science Ltd</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-5b99fb3a0b80eacd475d7cdefaeb8c351515ed9608d80fa4df0802862388cfa33</citedby><cites>FETCH-LOGICAL-c420t-5b99fb3a0b80eacd475d7cdefaeb8c351515ed9608d80fa4df0802862388cfa33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0956566398000657$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1697206$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9842700$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Laricchia-Robbio, Leopoldo</creatorcontrib><creatorcontrib>Balzan, Silvana</creatorcontrib><creatorcontrib>Ghione, Sergio</creatorcontrib><creatorcontrib>Montali, Umberto</creatorcontrib><creatorcontrib>Revoltella, Roberto P</creatorcontrib><title>Detection of digitalis compounds using a surface plasmon resonance-based biosensor</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>An automated surface plasmon resonance-based biosensor system has been used to detect endogenous and exogenous digitalis-like factors (EDLF) in the pmolar range in real time. EDLF was purified from umbilical cord blood. EDLF has been suggested to play a role in hypertension and in perinatal adaptation. Highly specific polyclonal anti-ouabain antibodies showed a high affinity binding capacity for ouabain, ouabagenin and strophantidin with an IC
50 value of 5×10
−10 M, 7.0×10
−10 M and 2×10
−8 M, respectively. EDLF cross-reacted with antibodies and its concentration in plasma at IC
50 was around 50 pmol ouabain equivalent. This study shows the potential usefulness of the biosensor technology for biomolecular interaction analysis. The features of this technology (fully automated, measured in real time, sharpened response) offer several advantages compared with a traditional immunoassay like radioimmunoassay (RIA) in the detection of digitalis compounds in human fluids.</description><subject>Analytical biochemistry: general aspects, technics, instrumentation</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Antibodies - blood</subject><subject>antiouabain antibodies</subject><subject>BIA technology</subject><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Cardenolides</subject><subject>Digoxin</subject><subject>endogenous digitalis-like factor</subject><subject>Enzyme Inhibitors - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Methods. Procedures. Technologies</subject><subject>Ouabain - immunology</subject><subject>Radioimmunoassay</subject><subject>RIA</subject><subject>Saponins - analysis</subject><subject>Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors</subject><subject>Surface Plasmon Resonance</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtrHTEMRk1pSG7T_oTALEJpF5NqHn6tSsijDQQKfayNx5aDw8z4xpoJ9N_Xyb0ky6KFFjqfJA5jJw2cNdCIL79Ac1FzIbpPWn0GAMFr-YZtGiW7um87_pZtXpAj9o7ovkCy0XDIDrXqWwmwYT8vcUG3xDRXKVQ-3sXFjpEql6ZtWmdP1UpxvqtsRWsO1mG1HS1NBc9Iabazw3qwhL4aYiKcKeX37CDYkfDDvh-zP9dXvy--17c_vt1cnN_Wrm9hqfmgdRg6C4MCtM73knvpPAaLg3Idb0qh1wKUVxBs7wMoaJVoO6VcsF13zD7u9m5zeliRFjNFcjiOdsa0kmllr7lseQH5DnQ5EWUMZpvjZPNf04B5cmmeXZonUUYr8-zSyJI72R9Yhwn9S2ovr8xP93NLzo4hFxuRXpcLLVsQBfu6w7DIeIyYDbmIRZyPuag3PsX_PPIPE-yR0g</recordid><startdate>19981101</startdate><enddate>19981101</enddate><creator>Laricchia-Robbio, Leopoldo</creator><creator>Balzan, Silvana</creator><creator>Ghione, Sergio</creator><creator>Montali, Umberto</creator><creator>Revoltella, Roberto P</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope></search><sort><creationdate>19981101</creationdate><title>Detection of digitalis compounds using a surface plasmon resonance-based biosensor</title><author>Laricchia-Robbio, Leopoldo ; Balzan, Silvana ; Ghione, Sergio ; Montali, Umberto ; Revoltella, Roberto P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-5b99fb3a0b80eacd475d7cdefaeb8c351515ed9608d80fa4df0802862388cfa33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Analytical biochemistry: general aspects, technics, instrumentation</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Antibodies - blood</topic><topic>antiouabain antibodies</topic><topic>BIA technology</topic><topic>Biological and medical sciences</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Cardenolides</topic><topic>Digoxin</topic><topic>endogenous digitalis-like factor</topic><topic>Enzyme Inhibitors - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Methods. Procedures. Technologies</topic><topic>Ouabain - immunology</topic><topic>Radioimmunoassay</topic><topic>RIA</topic><topic>Saponins - analysis</topic><topic>Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors</topic><topic>Surface Plasmon Resonance</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laricchia-Robbio, Leopoldo</creatorcontrib><creatorcontrib>Balzan, Silvana</creatorcontrib><creatorcontrib>Ghione, Sergio</creatorcontrib><creatorcontrib>Montali, Umberto</creatorcontrib><creatorcontrib>Revoltella, Roberto P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laricchia-Robbio, Leopoldo</au><au>Balzan, Silvana</au><au>Ghione, Sergio</au><au>Montali, Umberto</au><au>Revoltella, Roberto P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of digitalis compounds using a surface plasmon resonance-based biosensor</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>1998-11-01</date><risdate>1998</risdate><volume>13</volume><issue>10</issue><spage>1055</spage><epage>1060</epage><pages>1055-1060</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>An automated surface plasmon resonance-based biosensor system has been used to detect endogenous and exogenous digitalis-like factors (EDLF) in the pmolar range in real time. EDLF was purified from umbilical cord blood. EDLF has been suggested to play a role in hypertension and in perinatal adaptation. Highly specific polyclonal anti-ouabain antibodies showed a high affinity binding capacity for ouabain, ouabagenin and strophantidin with an IC
50 value of 5×10
−10 M, 7.0×10
−10 M and 2×10
−8 M, respectively. EDLF cross-reacted with antibodies and its concentration in plasma at IC
50 was around 50 pmol ouabain equivalent. This study shows the potential usefulness of the biosensor technology for biomolecular interaction analysis. The features of this technology (fully automated, measured in real time, sharpened response) offer several advantages compared with a traditional immunoassay like radioimmunoassay (RIA) in the detection of digitalis compounds in human fluids.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><pmid>9842700</pmid><doi>10.1016/S0956-5663(98)00065-7</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Antibodies - blood antiouabain antibodies BIA technology Biological and medical sciences Biosensors Biotechnology Cardenolides Digoxin endogenous digitalis-like factor Enzyme Inhibitors - analysis Fundamental and applied biological sciences. Psychology Humans Infant, Newborn Methods. Procedures. Technologies Ouabain - immunology Radioimmunoassay RIA Saponins - analysis Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors Surface Plasmon Resonance Various methods and equipments |
| title | Detection of digitalis compounds using a surface plasmon resonance-based biosensor |
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