Message to researchers: the characteristic absence of a posterior communicating artery is easily lost in the gerbil
The Mongolian gerbil has historically been useful for brain ischemia experiments, owing to the gerbil’s uniquely underdeveloped circle of Willis (CoW). This led to a gerbil model of cochlear ischemia being generated in our unit. However, we have found that the usual severe hearing loss seen in this...
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| Veröffentlicht in: | Anatomical science international 2023-07, Vol.98 (3), p.426-433 |
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| creator | Abe, Yasunori Toyama, Kensuke Shinohara, Akio Nagura-Kato, Goro A. Ikai, Yuki Koshimoto, Chihiro Spin, Joshua M. Hato, Naohito |
| description | The Mongolian gerbil has historically been useful for brain ischemia experiments, owing to the gerbil’s uniquely underdeveloped circle of Willis (CoW). This led to a gerbil model of cochlear ischemia being generated in our unit. However, we have found that the usual severe hearing loss seen in this model was not being induced consistently in recent experiments using the MON/Jms/GbsSlc gerbil (the sole commercially available gerbil in Japan). We set out to evaluate the posterior communicating artery (PcomA) in MON/Jms/GbsSlc, to re-establish whether this strain is appropriate for ischemia models. Having found that this unique feature is often lost, we then attempted to breed for the characteristic absent PcomA. India-ink perfusion revealed that the percentage of intact bilateral PcomA (“communicating type”) in the MON/Jms/GbsSlc gerbil was 57%; unilateral only (“unilateral communicating type”) was 39%; and completely absent PcomA (“non-communicating type”) was 4%. We were able to obtain few examples of the indigenous old aged Japanese UNG/Mz gerbil strain (at University of Miyazaki). Unfortunately, the pure UNG/Mz female was too elderly for mating. Therefore, selective breeding crosses between MON/Jms/GbsSlc and male UNG/Mz were carried out. After five generations of selective breeding, the percentage of non-communicating type gerbils was significantly higher in the newly generated strain, MON/Jms/SlcMz (F6 generation; 63%) than in the MON/Jms/GbsSlc gerbil. Bilateral common carotid artery occlusion surgery demonstrated that the cerebral blood flow was significantly reduced in MON/Jms/SlcMz compared with MON/Jms/GbsSlc (
p
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| doi_str_mv | 10.1007/s12565-022-00698-z |
| format | Article |
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p
< 0.0001) and induced more hippocampal injuries in MON/Jms/SlcMz than in MON/Jms/GbsSlc (
p
< 0.01). In conclusion, the commercially available MON/Jms/GbsSlc gerbil can easily regain PcomA, and we established a new gerbil strain (MON/Jms/SlcMz) displaying non-PcomA.</description><identifier>ISSN: 1447-6959</identifier><identifier>EISSN: 1447-073X</identifier><identifier>DOI: 10.1007/s12565-022-00698-z</identifier><identifier>PMID: 36472757</identifier><language>eng</language><publisher>Singapore: Springer Nature Singapore</publisher><subject>Anatomy ; Animal Anatomy ; Animal Physiology ; Blood flow ; Brain injury ; Breeding ; Carotid artery ; Cell Biology ; Cerebral blood flow ; Cochlea ; Hearing loss ; Hippocampus ; Histology ; Human Physiology ; Ischemia ; Medicine ; Medicine & Public Health ; Morphology ; Neurosciences ; Original Article ; Veins & arteries</subject><ispartof>Anatomical science international, 2023-07, Vol.98 (3), p.426-433</ispartof><rights>The Author(s), under exclusive licence to Japanese Association of Anatomists 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2022. The Author(s), under exclusive licence to Japanese Association of Anatomists.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-d4634046e56bc20866136b0cbfd9edb7f4aa1c20e089d86faf7f16f58aa9cfa93</citedby><cites>FETCH-LOGICAL-c466t-d4634046e56bc20866136b0cbfd9edb7f4aa1c20e089d86faf7f16f58aa9cfa93</cites><orcidid>0000-0002-3959-4737</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12565-022-00698-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12565-022-00698-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36472757$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abe, Yasunori</creatorcontrib><creatorcontrib>Toyama, Kensuke</creatorcontrib><creatorcontrib>Shinohara, Akio</creatorcontrib><creatorcontrib>Nagura-Kato, Goro A.</creatorcontrib><creatorcontrib>Ikai, Yuki</creatorcontrib><creatorcontrib>Koshimoto, Chihiro</creatorcontrib><creatorcontrib>Spin, Joshua M.</creatorcontrib><creatorcontrib>Hato, Naohito</creatorcontrib><title>Message to researchers: the characteristic absence of a posterior communicating artery is easily lost in the gerbil</title><title>Anatomical science international</title><addtitle>Anat Sci Int</addtitle><addtitle>Anat Sci Int</addtitle><description>The Mongolian gerbil has historically been useful for brain ischemia experiments, owing to the gerbil’s uniquely underdeveloped circle of Willis (CoW). This led to a gerbil model of cochlear ischemia being generated in our unit. However, we have found that the usual severe hearing loss seen in this model was not being induced consistently in recent experiments using the MON/Jms/GbsSlc gerbil (the sole commercially available gerbil in Japan). We set out to evaluate the posterior communicating artery (PcomA) in MON/Jms/GbsSlc, to re-establish whether this strain is appropriate for ischemia models. Having found that this unique feature is often lost, we then attempted to breed for the characteristic absent PcomA. India-ink perfusion revealed that the percentage of intact bilateral PcomA (“communicating type”) in the MON/Jms/GbsSlc gerbil was 57%; unilateral only (“unilateral communicating type”) was 39%; and completely absent PcomA (“non-communicating type”) was 4%. We were able to obtain few examples of the indigenous old aged Japanese UNG/Mz gerbil strain (at University of Miyazaki). Unfortunately, the pure UNG/Mz female was too elderly for mating. Therefore, selective breeding crosses between MON/Jms/GbsSlc and male UNG/Mz were carried out. After five generations of selective breeding, the percentage of non-communicating type gerbils was significantly higher in the newly generated strain, MON/Jms/SlcMz (F6 generation; 63%) than in the MON/Jms/GbsSlc gerbil. Bilateral common carotid artery occlusion surgery demonstrated that the cerebral blood flow was significantly reduced in MON/Jms/SlcMz compared with MON/Jms/GbsSlc (
p
< 0.0001) and induced more hippocampal injuries in MON/Jms/SlcMz than in MON/Jms/GbsSlc (
p
< 0.01). In conclusion, the commercially available MON/Jms/GbsSlc gerbil can easily regain PcomA, and we established a new gerbil strain (MON/Jms/SlcMz) displaying non-PcomA.</description><subject>Anatomy</subject><subject>Animal Anatomy</subject><subject>Animal Physiology</subject><subject>Blood flow</subject><subject>Brain injury</subject><subject>Breeding</subject><subject>Carotid artery</subject><subject>Cell Biology</subject><subject>Cerebral blood flow</subject><subject>Cochlea</subject><subject>Hearing loss</subject><subject>Hippocampus</subject><subject>Histology</subject><subject>Human Physiology</subject><subject>Ischemia</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Morphology</subject><subject>Neurosciences</subject><subject>Original Article</subject><subject>Veins & arteries</subject><issn>1447-6959</issn><issn>1447-073X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kU9v1DAQxS0EoqXwBTggS1y4BPwvdswNVbQgFXFpJW6W44x3XSXx4kkO20-Pu7uAxIHTWPN-88aaR8hrzt5zxswH5KLVbcOEaBjTtmsenpBzrpRpmJE_np7e2rb2jLxAvGeM25bL5-RMamWEac05wW-A6DdAl0wLIPgStlDwI122QMPWFx8WKAmXFKjvEeYANEfq6S7jo5ALDXma1jkFv6R5Q32p7T1NSMFjGvd0rCBN88FwA6VP40vyLPoR4dWpXpC7q8-3l1-am-_XXy8_3TRBab00g9JSMaWh1X0QrNOaS92z0MfBwtCbqLznVQDW2aHT0UcTuY5t570N0Vt5Qd4dfXcl_1wBFzclDDCOfoa8ohPmcAUrREXf_oPe57XM9XdOdEJJabRhlRJHKpSMWCC6XUmTL3vHmXuMxB0jcTUSd4jEPdShNyfrtZ9g-DPyO4MKyCOAVZrrif7u_o_tL7O5mWk</recordid><startdate>20230701</startdate><enddate>20230701</enddate><creator>Abe, Yasunori</creator><creator>Toyama, Kensuke</creator><creator>Shinohara, Akio</creator><creator>Nagura-Kato, Goro A.</creator><creator>Ikai, Yuki</creator><creator>Koshimoto, Chihiro</creator><creator>Spin, Joshua M.</creator><creator>Hato, Naohito</creator><general>Springer Nature Singapore</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3959-4737</orcidid></search><sort><creationdate>20230701</creationdate><title>Message to researchers: the characteristic absence of a posterior communicating artery is easily lost in the gerbil</title><author>Abe, Yasunori ; Toyama, Kensuke ; Shinohara, Akio ; Nagura-Kato, Goro A. ; Ikai, Yuki ; Koshimoto, Chihiro ; Spin, Joshua M. ; Hato, Naohito</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-d4634046e56bc20866136b0cbfd9edb7f4aa1c20e089d86faf7f16f58aa9cfa93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Anatomy</topic><topic>Animal Anatomy</topic><topic>Animal Physiology</topic><topic>Blood flow</topic><topic>Brain injury</topic><topic>Breeding</topic><topic>Carotid artery</topic><topic>Cell Biology</topic><topic>Cerebral blood flow</topic><topic>Cochlea</topic><topic>Hearing loss</topic><topic>Hippocampus</topic><topic>Histology</topic><topic>Human Physiology</topic><topic>Ischemia</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Morphology</topic><topic>Neurosciences</topic><topic>Original Article</topic><topic>Veins & arteries</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abe, Yasunori</creatorcontrib><creatorcontrib>Toyama, Kensuke</creatorcontrib><creatorcontrib>Shinohara, Akio</creatorcontrib><creatorcontrib>Nagura-Kato, Goro A.</creatorcontrib><creatorcontrib>Ikai, Yuki</creatorcontrib><creatorcontrib>Koshimoto, Chihiro</creatorcontrib><creatorcontrib>Spin, Joshua M.</creatorcontrib><creatorcontrib>Hato, Naohito</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Anatomical science international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abe, Yasunori</au><au>Toyama, Kensuke</au><au>Shinohara, Akio</au><au>Nagura-Kato, Goro A.</au><au>Ikai, Yuki</au><au>Koshimoto, Chihiro</au><au>Spin, Joshua M.</au><au>Hato, Naohito</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Message to researchers: the characteristic absence of a posterior communicating artery is easily lost in the gerbil</atitle><jtitle>Anatomical science international</jtitle><stitle>Anat Sci Int</stitle><addtitle>Anat Sci Int</addtitle><date>2023-07-01</date><risdate>2023</risdate><volume>98</volume><issue>3</issue><spage>426</spage><epage>433</epage><pages>426-433</pages><issn>1447-6959</issn><eissn>1447-073X</eissn><abstract>The Mongolian gerbil has historically been useful for brain ischemia experiments, owing to the gerbil’s uniquely underdeveloped circle of Willis (CoW). This led to a gerbil model of cochlear ischemia being generated in our unit. However, we have found that the usual severe hearing loss seen in this model was not being induced consistently in recent experiments using the MON/Jms/GbsSlc gerbil (the sole commercially available gerbil in Japan). We set out to evaluate the posterior communicating artery (PcomA) in MON/Jms/GbsSlc, to re-establish whether this strain is appropriate for ischemia models. Having found that this unique feature is often lost, we then attempted to breed for the characteristic absent PcomA. India-ink perfusion revealed that the percentage of intact bilateral PcomA (“communicating type”) in the MON/Jms/GbsSlc gerbil was 57%; unilateral only (“unilateral communicating type”) was 39%; and completely absent PcomA (“non-communicating type”) was 4%. We were able to obtain few examples of the indigenous old aged Japanese UNG/Mz gerbil strain (at University of Miyazaki). Unfortunately, the pure UNG/Mz female was too elderly for mating. Therefore, selective breeding crosses between MON/Jms/GbsSlc and male UNG/Mz were carried out. After five generations of selective breeding, the percentage of non-communicating type gerbils was significantly higher in the newly generated strain, MON/Jms/SlcMz (F6 generation; 63%) than in the MON/Jms/GbsSlc gerbil. Bilateral common carotid artery occlusion surgery demonstrated that the cerebral blood flow was significantly reduced in MON/Jms/SlcMz compared with MON/Jms/GbsSlc (
p
< 0.0001) and induced more hippocampal injuries in MON/Jms/SlcMz than in MON/Jms/GbsSlc (
p
< 0.01). In conclusion, the commercially available MON/Jms/GbsSlc gerbil can easily regain PcomA, and we established a new gerbil strain (MON/Jms/SlcMz) displaying non-PcomA.</abstract><cop>Singapore</cop><pub>Springer Nature Singapore</pub><pmid>36472757</pmid><doi>10.1007/s12565-022-00698-z</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-3959-4737</orcidid></addata></record> |
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| subjects | Anatomy Animal Anatomy Animal Physiology Blood flow Brain injury Breeding Carotid artery Cell Biology Cerebral blood flow Cochlea Hearing loss Hippocampus Histology Human Physiology Ischemia Medicine Medicine & Public Health Morphology Neurosciences Original Article Veins & arteries |
| title | Message to researchers: the characteristic absence of a posterior communicating artery is easily lost in the gerbil |
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