Exploring the mechanism of MP gel against skin photoaging based on network pharmacology, molecular docking, and experimental validation
Objective Long‐term and high exposure to UV radiation can lead to the development of skin photoaging diseases. Therefore, there is an ongoing need for more natural and safe drugs to prevent or treat skin photoaging diseases. Methods The Traditional Chinese Medicine Systems Pharmacology Database and...
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| Veröffentlicht in: | Journal of cosmetic dermatology 2023-03, Vol.22 (3), p.1108-1123 |
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| container_title | Journal of cosmetic dermatology |
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| creator | Xu, Jinfan Wu, Guodong Yu, Xianglin Dong, Zhiheng Yan, Jibiao Wu, Lan Bao, Lidao Liu, Quanli |
| description | Objective
Long‐term and high exposure to UV radiation can lead to the development of skin photoaging diseases. Therefore, there is an ongoing need for more natural and safe drugs to prevent or treat skin photoaging diseases.
Methods
The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform database were used to collect the active compounds and corresponding targets of Cnidii Fructus, Arnebiae Radix, Angelicae Sinensis Radix, Poria, and Borneolum. The GeneCards database and the NCBI Gene database were used to collect the targets of skin photoaging diseases. The STRING database was used to construct a protein–protein interaction network formed by the intersecting targets of drugs and diseases. The Metascape database was applied for Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of the targets. Molecular docking between active compounds and targets was verified by Autodock. After that, the skin photoaging model of mice was established and treated with MP gel. The skin characterization on the back of mice was observed, and the ameliorative effect of MP gel on skin photoaging was evaluated by histological and epidermal thickness assays. The MDA content and SOD activity were measured. Caspase‐3 expression in mouse skin tissues was detected by immunohistochemistry, quantitative real‐time polymerase chain reaction assay, and Western blot.
Results
The results of network pharmacology experiments showed that the natural drugs have multi‐component, multi‐target therapeutic disease characteristics. The results of animal studies showed that MP gel improved the health of photoaged skin, promoted skin structural integrity, had antioxidant properties and significantly inhibited caspase‐3 expression.
Conclusion
The experimental validation of the results of the preliminary network pharmacology analysis was carried out in animal experiments, which confirmed part of the mechanism of action of MP gel in the prevention and treatment of skin photoaging. |
| doi_str_mv | 10.1111/jocd.15542 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2747007903</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3090613081</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3932-526e645270a84472f2823c72cbff30a8abd575744150d95aca2987a3d45c6cb93</originalsourceid><addsrcrecordid>eNp9kU1v1DAQhi1ERUvhwg9AlrhUVbf4M94c0bZ8VEXlAGdr4jhZbx072Enb_QX8bbxs6YEDvow188yrmXkRekPJOS3v_Saa9pxKKdgzdESF4gtGK_n86c_JIXqZ84YQqmoqX6BDXolKEi6O0K_Lh9HH5EKPp7XFgzVrCC4POHb46zfcW4-hBxfyhPOtC3hcxylCv-MbyLbFMeBgp_uYbksN0gAm-thvz_AQvTWzh4TbaEprf4YhtNg-jDa5wYYJPL4D71qYXAyv0EEHPtvXj_EY_fh4-X31eXF98-nL6sP1wvCas4Vkla2EZIrAUgjFOrZk3Chmmq7jJQdNK5VUQlBJ2lqCAVYvFfBWSFOZpubH6GSvO6b4c7Z50oPLxnoPwcY5a6aEIkTVhBf03T_oJs4plOk0JzWpyl2XtFCne8qkmHOynR7LepC2mhK9s0fv7NF_7Cnw20fJuRls-4T-9aMAdA_cO2-3_5HSVzeri73obyGxmxo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3090613081</pqid></control><display><type>article</type><title>Exploring the mechanism of MP gel against skin photoaging based on network pharmacology, molecular docking, and experimental validation</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Wiley-Blackwell Open Access Titles</source><creator>Xu, Jinfan ; Wu, Guodong ; Yu, Xianglin ; Dong, Zhiheng ; Yan, Jibiao ; Wu, Lan ; Bao, Lidao ; Liu, Quanli</creator><creatorcontrib>Xu, Jinfan ; Wu, Guodong ; Yu, Xianglin ; Dong, Zhiheng ; Yan, Jibiao ; Wu, Lan ; Bao, Lidao ; Liu, Quanli</creatorcontrib><description>Objective
Long‐term and high exposure to UV radiation can lead to the development of skin photoaging diseases. Therefore, there is an ongoing need for more natural and safe drugs to prevent or treat skin photoaging diseases.
Methods
The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform database were used to collect the active compounds and corresponding targets of Cnidii Fructus, Arnebiae Radix, Angelicae Sinensis Radix, Poria, and Borneolum. The GeneCards database and the NCBI Gene database were used to collect the targets of skin photoaging diseases. The STRING database was used to construct a protein–protein interaction network formed by the intersecting targets of drugs and diseases. The Metascape database was applied for Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of the targets. Molecular docking between active compounds and targets was verified by Autodock. After that, the skin photoaging model of mice was established and treated with MP gel. The skin characterization on the back of mice was observed, and the ameliorative effect of MP gel on skin photoaging was evaluated by histological and epidermal thickness assays. The MDA content and SOD activity were measured. Caspase‐3 expression in mouse skin tissues was detected by immunohistochemistry, quantitative real‐time polymerase chain reaction assay, and Western blot.
Results
The results of network pharmacology experiments showed that the natural drugs have multi‐component, multi‐target therapeutic disease characteristics. The results of animal studies showed that MP gel improved the health of photoaged skin, promoted skin structural integrity, had antioxidant properties and significantly inhibited caspase‐3 expression.
Conclusion
The experimental validation of the results of the preliminary network pharmacology analysis was carried out in animal experiments, which confirmed part of the mechanism of action of MP gel in the prevention and treatment of skin photoaging.</description><identifier>ISSN: 1473-2130</identifier><identifier>EISSN: 1473-2165</identifier><identifier>DOI: 10.1111/jocd.15542</identifier><identifier>PMID: 36465034</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Aging ; Animal research ; Animals ; Cancer therapies ; Caspase 3 ; Chinese medicine ; Extracellular matrix ; Fermentation ; Genes ; Herbs ; Mice ; Molecular Docking Simulation ; Network Pharmacology ; Open source software ; Oxidative stress ; Pharmacology ; Probiotics ; Proteins ; Radiation ; Skin ; Skin Aging ; Skin diseases ; skin photoaging ; Transcription factors ; ultraviolet</subject><ispartof>Journal of cosmetic dermatology, 2023-03, Vol.22 (3), p.1108-1123</ispartof><rights>2022 The Authors. published by Wiley Periodicals LLC.</rights><rights>2022 The Authors. Journal of Cosmetic Dermatology published by Wiley Periodicals LLC.</rights><rights>2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3932-526e645270a84472f2823c72cbff30a8abd575744150d95aca2987a3d45c6cb93</citedby><cites>FETCH-LOGICAL-c3932-526e645270a84472f2823c72cbff30a8abd575744150d95aca2987a3d45c6cb93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjocd.15542$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjocd.15542$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1416,11560,27922,27923,45572,45573,46050,46474</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36465034$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Jinfan</creatorcontrib><creatorcontrib>Wu, Guodong</creatorcontrib><creatorcontrib>Yu, Xianglin</creatorcontrib><creatorcontrib>Dong, Zhiheng</creatorcontrib><creatorcontrib>Yan, Jibiao</creatorcontrib><creatorcontrib>Wu, Lan</creatorcontrib><creatorcontrib>Bao, Lidao</creatorcontrib><creatorcontrib>Liu, Quanli</creatorcontrib><title>Exploring the mechanism of MP gel against skin photoaging based on network pharmacology, molecular docking, and experimental validation</title><title>Journal of cosmetic dermatology</title><addtitle>J Cosmet Dermatol</addtitle><description>Objective
Long‐term and high exposure to UV radiation can lead to the development of skin photoaging diseases. Therefore, there is an ongoing need for more natural and safe drugs to prevent or treat skin photoaging diseases.
Methods
The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform database were used to collect the active compounds and corresponding targets of Cnidii Fructus, Arnebiae Radix, Angelicae Sinensis Radix, Poria, and Borneolum. The GeneCards database and the NCBI Gene database were used to collect the targets of skin photoaging diseases. The STRING database was used to construct a protein–protein interaction network formed by the intersecting targets of drugs and diseases. The Metascape database was applied for Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of the targets. Molecular docking between active compounds and targets was verified by Autodock. After that, the skin photoaging model of mice was established and treated with MP gel. The skin characterization on the back of mice was observed, and the ameliorative effect of MP gel on skin photoaging was evaluated by histological and epidermal thickness assays. The MDA content and SOD activity were measured. Caspase‐3 expression in mouse skin tissues was detected by immunohistochemistry, quantitative real‐time polymerase chain reaction assay, and Western blot.
Results
The results of network pharmacology experiments showed that the natural drugs have multi‐component, multi‐target therapeutic disease characteristics. The results of animal studies showed that MP gel improved the health of photoaged skin, promoted skin structural integrity, had antioxidant properties and significantly inhibited caspase‐3 expression.
Conclusion
The experimental validation of the results of the preliminary network pharmacology analysis was carried out in animal experiments, which confirmed part of the mechanism of action of MP gel in the prevention and treatment of skin photoaging.</description><subject>Aging</subject><subject>Animal research</subject><subject>Animals</subject><subject>Cancer therapies</subject><subject>Caspase 3</subject><subject>Chinese medicine</subject><subject>Extracellular matrix</subject><subject>Fermentation</subject><subject>Genes</subject><subject>Herbs</subject><subject>Mice</subject><subject>Molecular Docking Simulation</subject><subject>Network Pharmacology</subject><subject>Open source software</subject><subject>Oxidative stress</subject><subject>Pharmacology</subject><subject>Probiotics</subject><subject>Proteins</subject><subject>Radiation</subject><subject>Skin</subject><subject>Skin Aging</subject><subject>Skin diseases</subject><subject>skin photoaging</subject><subject>Transcription factors</subject><subject>ultraviolet</subject><issn>1473-2130</issn><issn>1473-2165</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp9kU1v1DAQhi1ERUvhwg9AlrhUVbf4M94c0bZ8VEXlAGdr4jhZbx072Enb_QX8bbxs6YEDvow188yrmXkRekPJOS3v_Saa9pxKKdgzdESF4gtGK_n86c_JIXqZ84YQqmoqX6BDXolKEi6O0K_Lh9HH5EKPp7XFgzVrCC4POHb46zfcW4-hBxfyhPOtC3hcxylCv-MbyLbFMeBgp_uYbksN0gAm-thvz_AQvTWzh4TbaEprf4YhtNg-jDa5wYYJPL4D71qYXAyv0EEHPtvXj_EY_fh4-X31eXF98-nL6sP1wvCas4Vkla2EZIrAUgjFOrZk3Chmmq7jJQdNK5VUQlBJ2lqCAVYvFfBWSFOZpubH6GSvO6b4c7Z50oPLxnoPwcY5a6aEIkTVhBf03T_oJs4plOk0JzWpyl2XtFCne8qkmHOynR7LepC2mhK9s0fv7NF_7Cnw20fJuRls-4T-9aMAdA_cO2-3_5HSVzeri73obyGxmxo</recordid><startdate>202303</startdate><enddate>202303</enddate><creator>Xu, Jinfan</creator><creator>Wu, Guodong</creator><creator>Yu, Xianglin</creator><creator>Dong, Zhiheng</creator><creator>Yan, Jibiao</creator><creator>Wu, Lan</creator><creator>Bao, Lidao</creator><creator>Liu, Quanli</creator><general>John Wiley & Sons, Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>202303</creationdate><title>Exploring the mechanism of MP gel against skin photoaging based on network pharmacology, molecular docking, and experimental validation</title><author>Xu, Jinfan ; Wu, Guodong ; Yu, Xianglin ; Dong, Zhiheng ; Yan, Jibiao ; Wu, Lan ; Bao, Lidao ; Liu, Quanli</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3932-526e645270a84472f2823c72cbff30a8abd575744150d95aca2987a3d45c6cb93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Aging</topic><topic>Animal research</topic><topic>Animals</topic><topic>Cancer therapies</topic><topic>Caspase 3</topic><topic>Chinese medicine</topic><topic>Extracellular matrix</topic><topic>Fermentation</topic><topic>Genes</topic><topic>Herbs</topic><topic>Mice</topic><topic>Molecular Docking Simulation</topic><topic>Network Pharmacology</topic><topic>Open source software</topic><topic>Oxidative stress</topic><topic>Pharmacology</topic><topic>Probiotics</topic><topic>Proteins</topic><topic>Radiation</topic><topic>Skin</topic><topic>Skin Aging</topic><topic>Skin diseases</topic><topic>skin photoaging</topic><topic>Transcription factors</topic><topic>ultraviolet</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Jinfan</creatorcontrib><creatorcontrib>Wu, Guodong</creatorcontrib><creatorcontrib>Yu, Xianglin</creatorcontrib><creatorcontrib>Dong, Zhiheng</creatorcontrib><creatorcontrib>Yan, Jibiao</creatorcontrib><creatorcontrib>Wu, Lan</creatorcontrib><creatorcontrib>Bao, Lidao</creatorcontrib><creatorcontrib>Liu, Quanli</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cosmetic dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Jinfan</au><au>Wu, Guodong</au><au>Yu, Xianglin</au><au>Dong, Zhiheng</au><au>Yan, Jibiao</au><au>Wu, Lan</au><au>Bao, Lidao</au><au>Liu, Quanli</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exploring the mechanism of MP gel against skin photoaging based on network pharmacology, molecular docking, and experimental validation</atitle><jtitle>Journal of cosmetic dermatology</jtitle><addtitle>J Cosmet Dermatol</addtitle><date>2023-03</date><risdate>2023</risdate><volume>22</volume><issue>3</issue><spage>1108</spage><epage>1123</epage><pages>1108-1123</pages><issn>1473-2130</issn><eissn>1473-2165</eissn><abstract>Objective
Long‐term and high exposure to UV radiation can lead to the development of skin photoaging diseases. Therefore, there is an ongoing need for more natural and safe drugs to prevent or treat skin photoaging diseases.
Methods
The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform database were used to collect the active compounds and corresponding targets of Cnidii Fructus, Arnebiae Radix, Angelicae Sinensis Radix, Poria, and Borneolum. The GeneCards database and the NCBI Gene database were used to collect the targets of skin photoaging diseases. The STRING database was used to construct a protein–protein interaction network formed by the intersecting targets of drugs and diseases. The Metascape database was applied for Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of the targets. Molecular docking between active compounds and targets was verified by Autodock. After that, the skin photoaging model of mice was established and treated with MP gel. The skin characterization on the back of mice was observed, and the ameliorative effect of MP gel on skin photoaging was evaluated by histological and epidermal thickness assays. The MDA content and SOD activity were measured. Caspase‐3 expression in mouse skin tissues was detected by immunohistochemistry, quantitative real‐time polymerase chain reaction assay, and Western blot.
Results
The results of network pharmacology experiments showed that the natural drugs have multi‐component, multi‐target therapeutic disease characteristics. The results of animal studies showed that MP gel improved the health of photoaged skin, promoted skin structural integrity, had antioxidant properties and significantly inhibited caspase‐3 expression.
Conclusion
The experimental validation of the results of the preliminary network pharmacology analysis was carried out in animal experiments, which confirmed part of the mechanism of action of MP gel in the prevention and treatment of skin photoaging.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>36465034</pmid><doi>10.1111/jocd.15542</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aging Animal research Animals Cancer therapies Caspase 3 Chinese medicine Extracellular matrix Fermentation Genes Herbs Mice Molecular Docking Simulation Network Pharmacology Open source software Oxidative stress Pharmacology Probiotics Proteins Radiation Skin Skin Aging Skin diseases skin photoaging Transcription factors ultraviolet |
| title | Exploring the mechanism of MP gel against skin photoaging based on network pharmacology, molecular docking, and experimental validation |
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